1.Preparation and application of monoclonal antibodies to recombinant human IFN alpha.
Meiying WU ; Yanping AI ; Ren REN ; Yixin LIANG ; Jie LI ; Wenguang SONG ; Dajun YANG ; Jianbo SUN ; Yongqing CHENG
Chinese Journal of Experimental and Clinical Virology 2002;16(3):261-263
OBJECTIVETo prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha).
METHODSFive cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique.
RESULTSAll the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%.
CONCLUSIONSThe highest purification efficiency was obtained by using 4G10 column.
Animals ; Antibodies, Monoclonal ; biosynthesis ; Antibody Affinity ; Antibody Specificity ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Hybridomas ; secretion ; Interferon Type I ; immunology ; isolation & purification ; Male ; Mice ; Mice, Inbred BALB C ; Recombinant Proteins
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