Objective　To investigate the distribution and antimicrobial resistance of bacteria isolated from celiac specimens in Sichuan region, and to provide a reference for the selection of clinical antibiotics. Methods　According to the technical protocol of the Sichuan Provincial Antimicrobial Resistance Technical Program, the data of bacteria isolated from celiac specimens and drug sensitivity results reported by members of Sichuan bacterial resistance monitoring network from 2019-2023 were statistically analyzed by WHONET 5.6 software, and only the first strain of the same bacteria from the same patient was included. Results　A total of 22 204 non-repetitive bacterial isolates were obtained from celiac specimens in Sichuan region from 2019 to 2023, including 6 023 Gram-positive bacteria (27.1%) and 16 181 Gram-negative bacteria (72.9%). The top 10 bacteria in celiac specimens were Escherichia coli 45.8% (10 179/22 204), Klebsiella pneumoniae 8.1% (1 788/22 204), Enterococcus faecium 6.1% (1 361/22 204), Pseudomonas aeruginosa 5.8% (1 289/22 204), Enterococcus faecalis 3.0% (676/22 204), Streptococcus anginosus 2.8% (624/22 204), Staphylococcus aureus 2.2% (484/22 204), Staphylococcus epidermidis 2.1% (473/22 204), Enterobacter cloacae 2.0% (439/22 204), and Streptococcus constellatus 1.8% (399/22 204). The results of in vitro antimicrobial susceptibility testing showed that from 2019 to 2023, the resistance rates of Escherichia coli(E.coli) to ciprofloxacin and levofloxacin remained stable (average resistance rates were 42.5% and 38.8% respectively), with a decreasing trend in the rate of their resistance to third-generation cephalosporins, and carbapenem-resistant Escherichia coli was 0.7%-0.8%. Klebsiella pneumoniae had five-year average resistance rates of 24.2%, 28.3%, and 17.8% to ceftriaxone, cefotaxime, and ceftazidime, respectively, with 8.2%-9.9% carbapenem-resistant Klebsiella pneumoniae. The 5-year average resistance rate of Pseudomonas aeruginosa showed average resistance rates over five years not exceeding 5.0% to carbapenems, quinolones, ceftazidime, and piperacillin/tazobactam. Enterococcus faecium had average resistance rates over 80.0% to ciprofloxacin, levofloxacin, and ampicillin, while Enterococcus faecalis showed average resistance rates below 24.0% to these three antimicrobial drugs. The average detection rate of methicillin-resistant Staphylococcus aureus was 33.9%, while the average detection rates of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis were 0.8% and 0.1%, respectively. Conclusions　Among the bacteria isolated from celiac specimens in Sichuan region, the ratio of Gram-negative to Gram-positive bacteria is about 7∶3, mainly including E.coli, K. pneumoniae, E. faecium, P. aeruginosa, and E.faecalis. In addition, carbapenems still maintain a high antibacterial activity in vitro against Enterobacterales and P. aeruginosa.