The quality of traditional Chinese medicine (TCM) prescriptions (TCMPs) is critical to clinical efficacy; however, evaluating their consistency and identifying sources of variability remain challenging. This study proposes an integrated strategy to assess the quality of 100 widely sold TCMPs. A "one-for-all" chromatographic method was employed to analyze 645 sample batches. This large-scale data collection enabled statistical evaluations, such as hierarchical cluster analysis (HCA) and similarity heatmap, to identify quality inconsistencies. The introduction of a TCM-specific mass spectrometry (MS) database allowed for rapid, automated annotation of chemicals across 100 prescriptions and facilitated the tracing of raw material sources. Results indicate that 19% of prescriptions exhibited chemical inconsistencies, which are associated with high market value, low pricing, and substantial price disparities. The MS database allowed rapid annotation of 761 and 673 compounds in positive and negative modes, respectively, in 100 TCMPs, with 73 prescriptions reported for the first time. The tracing efforts succeeded in identifying >40% of the raw material sources for 51 prescriptions. P93 (Yinianjin (YNJ)) is a case in which the chromatographic profiles from three manufacturers displayed inconsistencies. Analysis using the database traced divergent peaks to Rhei Radix et R hizoma (RRER). Verification with self-prepared samples confirmed that manufacturers utilized three distinct botanical sources. This integrated strategy provides a scalable framework for quality control in TCMPs.

Objective To investigate the differences of condylar position in patients with temporomandibular disorders(TMD),and to further explore the effects of condylar position displacement and malocclusion factors on TMD.Methods According to the inclusion criteria,normal occlusion subjects without TMD(group A,n=10),normal occlusion subjects with TMD(group B,n=8),malocclu-sion subjects without TMD(group C,n=9)and malocclusion subjects with TMD(group D,n=20)were collected.Condylar position measurement(CPM)and Cadiax Ⅳ were used to locate the position of condyle in RP and ICP,and the displacement and direction of condyle in three-dimensional direction from RP to ICP were measured by vernier caliper.All the data were averaged three times and an-alyzed by SPSS26.0 statistical software.Results In the sagittal direction,the condylar displacement of group A and group B,group C and group D,and group A and group D had statistical differences(P＜0.01).The displacement of the right condyle in the vertical direc-tion of group D was greater than that of group A,and the difference was statistically significant(P＜0.05).The difference among other groups was not statistically significant.There was no significant difference in the lateral displacement of condyle between the four groups.In group A and group D,the largest proportion of condyle deviation was anterior up,while in group B and group C,the largest proportion of condyle deviation was posterior up.Conclusion RP-ICP condylar sagittal displacement is common in people with TMD.The condyle displacement of RP-ICP in malocclusion patients without TMD is not significantly different from that in normal occlusion patients without TMD.Patients with TMD malocclusion are more likely to have condylar displacement in sagittal direction and vertical direction than normal occlusion without TMD.

Objective To investigate the differences of condylar position in patients with temporomandibular disorders(TMD),and to further explore the effects of condylar position displacement and malocclusion factors on TMD.Methods According to the inclusion criteria,normal occlusion subjects without TMD(group A,n=10),normal occlusion subjects with TMD(group B,n=8),malocclu-sion subjects without TMD(group C,n=9)and malocclusion subjects with TMD(group D,n=20)were collected.Condylar position measurement(CPM)and Cadiax Ⅳ were used to locate the position of condyle in RP and ICP,and the displacement and direction of condyle in three-dimensional direction from RP to ICP were measured by vernier caliper.All the data were averaged three times and an-alyzed by SPSS26.0 statistical software.Results In the sagittal direction,the condylar displacement of group A and group B,group C and group D,and group A and group D had statistical differences(P＜0.01).The displacement of the right condyle in the vertical direc-tion of group D was greater than that of group A,and the difference was statistically significant(P＜0.05).The difference among other groups was not statistically significant.There was no significant difference in the lateral displacement of condyle between the four groups.In group A and group D,the largest proportion of condyle deviation was anterior up,while in group B and group C,the largest proportion of condyle deviation was posterior up.Conclusion RP-ICP condylar sagittal displacement is common in people with TMD.The condyle displacement of RP-ICP in malocclusion patients without TMD is not significantly different from that in normal occlusion patients without TMD.Patients with TMD malocclusion are more likely to have condylar displacement in sagittal direction and vertical direction than normal occlusion without TMD.

Objective To investigate the impacts of percutaneous endoscopic interlaminar discectomy(PEID)on pain level and lumbar function in patients with lumbar disc herniation(LDH).Methods From January 2022 to August 2023,84 patients were diagnosed with L4/5 LDH based on clinical data and imaging information were selected as the study subjects.And they were assigned into 42 cases in experimental group and 42 cases in traditional groups complying with treatment methods.The traditional group underwent traditional open approach treatment,while the experimental group underwent PEID.The surgical indicators(including hospitalization time,surgical time,bed rest time,and intraoperative fluoroscopy frequency),the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were compared between the two groups,the visual analogue scale(VAS)score and Oswestry disability index(ODI)were used to quantify the pain level of waist and lower limbs and lumbar function,and the excellent and good rate of clinical satisfaction and the incidence of complications were compared.Results The hospitalization time and bed rest time of the experimental group were obviously shorter than those of the traditional group,while the fluoroscopy frequency was less than that of the traditional group,and surgical time was longer than that of the traditional group,the differences were statistically significant(P＜0.05).After treatment,the pain VAS score and ODI of waist and lower limbs,the IL-6,and TNF-α in the two groups were lower than those before treatment,the experimental group was lower than the traditional group,the differences were statistically significant(P＜0.05).The excellent and good rate of the experimental group and the traditional group were 88.10％and 83.33％,respectively,with no statistically significant difference(P＞0.05).There was no statistically significant difference in the total incidence of complications between the two groups(P＞0.05).Conclusion PEID surgery can effectively alleviate nerve compression,alleviate pain of waist and lower limbs,promote the recovering of lumbar function,and facilitate postoperative recovery in LDH patients.

Objective To investigate the impacts of percutaneous endoscopic interlaminar discectomy(PEID)on pain level and lumbar function in patients with lumbar disc herniation(LDH).Methods From January 2022 to August 2023,84 patients were diagnosed with L4/5 LDH based on clinical data and imaging information were selected as the study subjects.And they were assigned into 42 cases in experimental group and 42 cases in traditional groups complying with treatment methods.The traditional group underwent traditional open approach treatment,while the experimental group underwent PEID.The surgical indicators(including hospitalization time,surgical time,bed rest time,and intraoperative fluoroscopy frequency),the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were compared between the two groups,the visual analogue scale(VAS)score and Oswestry disability index(ODI)were used to quantify the pain level of waist and lower limbs and lumbar function,and the excellent and good rate of clinical satisfaction and the incidence of complications were compared.Results The hospitalization time and bed rest time of the experimental group were obviously shorter than those of the traditional group,while the fluoroscopy frequency was less than that of the traditional group,and surgical time was longer than that of the traditional group,the differences were statistically significant(P＜0.05).After treatment,the pain VAS score and ODI of waist and lower limbs,the IL-6,and TNF-α in the two groups were lower than those before treatment,the experimental group was lower than the traditional group,the differences were statistically significant(P＜0.05).The excellent and good rate of the experimental group and the traditional group were 88.10％and 83.33％,respectively,with no statistically significant difference(P＞0.05).There was no statistically significant difference in the total incidence of complications between the two groups(P＞0.05).Conclusion PEID surgery can effectively alleviate nerve compression,alleviate pain of waist and lower limbs,promote the recovering of lumbar function,and facilitate postoperative recovery in LDH patients.

Objective Stereotactic body radiotherapy(SBRT)and radiofrequency ablation(RFA)are primary treatments for hepatocellular carcinoma(HCC)at present.However,the effect of these treatments in clinical trails are rather controversial.The purpose of this paper is to conduct a meta-analysis on the clinical effect and related complications of SBRT and RFA for HCC.Methods A computerized retrieval of academic papers concerning the treatment effect of SBRT and RFA for HCC from the databases of PubMed,Embase,Cochrane Library,Scopus,Web of Science,CBM,CNKI,Wanfang database,VIP was conducted.The retrieval time period was from the establishment of the database to June 2022.Stata14.0 software was used to make meta-analysis.Results A total of 14 retrospective studies including 6 806 patients were included in this analysis.The results of combined hazard ratio(HR)based on overall survival(OS)showed that the OS rate of SBRT was lower than that of RFA(HR=1.25,95％CI=1.10-1.43,12=0％,P=0.000 9),while the results of combined HR of local control(LC)rate indicated that SBRT had a better therapeutic effect(HR=0.61,95％CI=0.47-0.78,I2=0％,P=0.000 1).Subgroup analysis revealed that the combined HR of LC rate favored the performance of SBRT for patients with tumor diameter larger than 2 cm(HR=2.64,95％CI=1.56-4.48,I=0％,P=0.000 3).No statistically significant difference in the incidence of late serious adverse reactions existed between SBRTgroup and RFA group(OR=1.01,95％CI=0.59-1.73,I2=30％,P=0.97).Conclusion SBRT is superior to RFA in controlling local HCC lesions,especially in patients whose tumor diameter is larger than 2 cm,although it does not show certain advantages in the survival benefit.(J Intervent Radiol,2023,32:1233-1241)

Objective:To explore the effect of scarred uterus and cesarean scar defect (CSD) on the reproductive outcomes of patients with previous cesarean delivery undergoing single embryo transfer (SET).Methods:A retrospective cohort study was conducted to analyze the data of patients with a history of cesarean delivery (CD) who accepted SET between January 2016 and April 2018 in Reproductive Medicine Center of the First Affiliated Hospital, Nanjing Medical University. According to the previous mode of delivery, patients were divided into CD group and vaginal delivery (VD) group. The pregnancy outcomes were analyzed in each embryo stage respectively. Furthermore, according to embryonic development stage, patients were divided into cleavage-stage embryo group (CD1 group, n=928 and VD1 group, n=928) and blastocyst-stage embryo group (CD2 group, n=570 and VD2 group, n=480). The CD population were further divided into two groups according to the presence of CSD to investigate the effect of CSD on pregnancy outcomes. Results:Regardless of women undergoing transfer of a cleavage-stage embryo or a blastocyst-stage embryo, there were no statistical differences in live birth rate, pregnancy rate, implantation rate, abortion rate and premature birth rate between CD group and VD group (all P>0.05). Compared with VD group, the risk of gestational complications such as placenta previa, postpartum hemorrhage, gestational diabetes, and gestational hypertension were not significantly increased in CD group (all P>0.05). The live birth rate was lower in CSD group than in non-CSD group in women undergoing the transfer of blastocyst-stage embryo [30.00% (12/40) vs. 51.32% (272/530), P=0.013]. The pregnancy rate and the implantation rate were lower in CSD group than in non-CSD group in women undergoing the transfer of the cleavage-stage embryo [18.97% (11/58) vs. 32.30% (281/870), P=0.034; 18.97% (11/58) vs. 31.26% (272/870), P=0.049]. Conclusion:With SET strategy, patients with a history of cesarean section have similar pregnancy outcomes to those with VD, and there is no increased risk of perinatal complications. However, the pregnancy outcomes of in vitro fertilization-embryo transfer are hampered in women with CSD. Single blastocyst-stage embryo transfer strategy is recommended for patients with scarred uterus.

Objective:To explore the effect of scarred uterus and cesarean scar defect (CSD) on the reproductive outcomes of patients with previous cesarean delivery undergoing single embryo transfer (SET).Methods:A retrospective cohort study was conducted to analyze the data of patients with a history of cesarean delivery (CD) who accepted SET between January 2016 and April 2018 in Reproductive Medicine Center of the First Affiliated Hospital, Nanjing Medical University. According to the previous mode of delivery, patients were divided into CD group and vaginal delivery (VD) group. The pregnancy outcomes were analyzed in each embryo stage respectively. Furthermore, according to embryonic development stage, patients were divided into cleavage-stage embryo group (CD1 group, n=928 and VD1 group, n=928) and blastocyst-stage embryo group (CD2 group, n=570 and VD2 group, n=480). The CD population were further divided into two groups according to the presence of CSD to investigate the effect of CSD on pregnancy outcomes. Results:Regardless of women undergoing transfer of a cleavage-stage embryo or a blastocyst-stage embryo, there were no statistical differences in live birth rate, pregnancy rate, implantation rate, abortion rate and premature birth rate between CD group and VD group (all P>0.05). Compared with VD group, the risk of gestational complications such as placenta previa, postpartum hemorrhage, gestational diabetes, and gestational hypertension were not significantly increased in CD group (all P>0.05). The live birth rate was lower in CSD group than in non-CSD group in women undergoing the transfer of blastocyst-stage embryo [30.00% (12/40) vs. 51.32% (272/530), P=0.013]. The pregnancy rate and the implantation rate were lower in CSD group than in non-CSD group in women undergoing the transfer of the cleavage-stage embryo [18.97% (11/58) vs. 32.30% (281/870), P=0.034; 18.97% (11/58) vs. 31.26% (272/870), P=0.049]. Conclusion:With SET strategy, patients with a history of cesarean section have similar pregnancy outcomes to those with VD, and there is no increased risk of perinatal complications. However, the pregnancy outcomes of in vitro fertilization-embryo transfer are hampered in women with CSD. Single blastocyst-stage embryo transfer strategy is recommended for patients with scarred uterus.

Objective:To analyze the relationship of hepatic transit time and portal vein pressure in the patients with portal hypertension and esophagogastric varices, and to clarify its clinical significance.Methods:A total of50hospitalized patients underwent esophagogastric venous embolization due to portal hypertension and esophagogastric varices were selected as observation group, and 50patients without history of liver disease and clinical manifestations of liver disease (hospitalized or out-patient) were selected as control group, All patients underwent liver contrast enhanced ultrasound.The free portal pressure (FPP) was measured by ultrasound guided portal vein puncture for operating pathways in the patients in observation group.The hepatic artery-hepatic vein transit time (HA-HVTT) , the hepatic portal vein-hepatic vein transit time (PV-HVTT) , the liver parenchymahepatic vein transit time (PA-HVTT) of the patients were compared between two groups.The correlations between HA-HVTT, PV-HVTT, PA-HVTT and FPP of the patients in observation group were analyzed.Results:The HA-HVTT, PV-HVTT and PA-HVTT of the patients in observation group were significantly shorter than those in control group (t=5.078, P＜0.01;t=12.163, P＜0.01;t=2.649, P＜0.01) .The HA-HVTT, PV-HVTT and PA-HVTT had negative correlations with FPP in observation group (r=-0.799, P＜0.01;r=-0.554, P＜0.01;r=-0.735, P＜0.01) .The linear relationship between HA-HVTT and FPP was Y=-0.410X+7.254;the linear relationship between PV-HVTT and FPP was Y=-0.355X+4.983;the linear relationship between PA-HVTT and FPP was Y=-0.566X+4.997.Conclusion:Liver transit time can be used as an effective index to judge the portal vein pressure, and it can provide the theroretical basis for the diagnosis and treatment of portal hypertension in the patients.

Objective To observe the curative effect of 17β-estradiol on idiopathic ventricular arrhythmia (IVA) during peri-menopausal period and explore the association with IP3/Ryn expression of receptor-regulated calcium pool. Methods 32 cases IVA during peri-menopausal period (IVA group) and 32 cases healthy women during peri-menopausal period of the same levels of age (normal control group) were selected. The IVA group received oral 17β-estradiol for 2 months. The count of arrhythmia in IVA group was recorded, the IP3/Ryn 2 expression pre- and post-treatment in IVA group and normal control group were detected. Results Compared with pre-treatment, 17β-estradiol significantly reduced the occurrence of IVA (P<0.05). Compared with pre-treatment, 17β-estradiol significantly reduced IP3 expression(P<0.05), while there was no significant difference compared with normal control group. 17β-estradiol had no significant effect on Ryn 2 expression. Conclusion The anti-IVA effect of 17β-estradiol may be associated with the IP3 receptor gene expression.