BackgroundAttention deficit hyperactivity disorder （ADHD） is a neurodevelopmental disorder characterized by age-inappropriate inattention， excessive activities incongruous with setting， and emotional impulsivity. Subthreshold ADHD （sADHD） is clinically defined as the presence of ADHD symptoms that do not meet the full diagnostic criteria for ADHD. Children with sADHD exhibit deficits in executive function， demonstrate more conduct， learning， and anxiety-related problems compared to typically developing children， and show even poorer working memory performance than children diagnosed with ADHD. Currently， there is limited epidemiological research on sADHD in China， with few studies simultaneously investigating the prevalence of both ADHD and sADHD in children. ObjectiveTo investigate the prevalence of ADHD and sADHD among children aged 6–13 years in Chongqing， analyzing their distribution characteristics within this population， with the aim of providing references for developing preventive measures against both ADHD and sADHD. MethodsFrom October to November 2023， a total of 3 398 students in grades 1–6 from six primary schools in Jiangbei District， Chongqing were selected using a stratified cluster random sampling method. The occurrence of ADHD and sADHD was evaluated by using the short version （18-item version） of the Swanson， Nolan， and Pelham IV rating scales （SNAP-IV） and the Chinese vision of Schedule for Affective Disorder and Schizophrenia for School-aged Children-Present and Lifetime Version （K-SADS-PL）. ResultsThe ADHD detection rate among children in Chongqing was 1.90% （95% CI： 0.014–0.024）. Boys showed a significantly higher ADHD detection rate than girls （χ²=7.733， P=0.005）. No statistically significant differences were found in ADHD detection rates across different grades or age groups （χ²=7.347， 12.362， P>0.05）. The sADHD detection rate was 6.32% （95% CI： 0.054–0.072）. Similarly， boys exhibited significantly higher sADHD detection rates than girls （χ²=21.005， P<0.01）. Significant differences emerged across different grades （χ²=20.559， P=0.001）， while no statistically significant difference was observed in age groups （χ²=12.070， P=0.060）. ConclusionThe ADHD detection rates were comparable across all grade levels and age groups from 6–13 years old. Second-grade children demonstrated notably higher sADHD rates compared to other grades， while boys demonstrated higher prevalence rates than girls for both ADHD and sADHD. ［Funded by Science and Health Joint Medical Research Project in Jiangbei District， Chongqing City in the Second Half of 2023 （number， 2023JBKWLH022）］

Objective To retrieve,evaluate and summarize the related evidences on non-pharmacological management of sarcopenia in community-dwelling older adults to provide an evidence-based basis for formula-ting a scientific and effective health management program for sarcopenia in older adults.Methods According to the"6S"evidence resource pyramid model,the UpToDate,JBI Center for Evidence-based Health Care,Co-chrane Library,DynaMed,Guidelines International Network,Registered Nurses'Association of Ontario,Med-Sci,Yimaitong,PubMed,CINAHL,Web of Science,CNKI,Wanfang,and CBM for evidence related to the non-pharmacological management of sarcopenia in older adults.The retrival time was from January 1,2018 to No-vember 1,2023.Results A total of 20 literatures were included,including 1 clinical decision,2 guidelines,7 expert consensuses and 10 systematic evaluations or meta analysis.Forty-two pieces of evidence were summa-rized in 4 aspects including screening and assessment,dietary and nutritional management,exercise guidance and health education,in which there were 25 recommendations for the level A and 17 recommendations for Level B.Conclusion Medical staff should select the best evidence by combining with the actual situation,medical conditions and individual factors of the patients,and guide elderly patients with sarcopenia to improve their lifestyle and quality of life safely and effectively.

Objective To understand the current situation of satisfaction and self-efficacy of nursing students in clinical practice and to explore the correlation between the two. Method A total of 120 nursing students in clinical practice were investigated using the general nursing student satisfaction scale and the general self-efficacy scale (GSES). Results The satisfaction score of nursing students was(151.89 ± 22.36)points and the score of nursing students self-efficacy was(29.06 ± 6.70)points.Nursing students self-efficacy was positively correlated with their job satisfaction (r=0.390,P＜0.01).Regression analysis showed that nursing students self-efficacy had a significant impact on job satisfaction (t=3.363,P＜0.01). Conclusion According to the characteristics of nursing students,clinical nursing education administrators should formulate and implement targeted interventions to deepen their love of nurses and improve their sense of self-efficacy,so as to enhance job satisfaction of nursing students.

Objective To investigate the expression of FHIT,WWOX and MDR1 gene in nasopharyngeal carcinoma and FHIT and WWOX mechanism of inactivation.Methods Real-time PCR was used to test FHIT,WWOX and MDR1 gene′s mRNA expression in 89 nasopharyngeal carcinoma patients (experimental group) and 61 inflammatory patients (control group).Q-MSP was used to test the FHIT and WWOX promoter methylation status.Denatured polyacrylamide gel electrophoresis was used to test the LOH of FHIT and WWOX gene.Results (1)The three genes′ mRNA expression were different between experimental group and control group (P<0.05).After grouped according to the histological type and clinical stages,the expression of FHIT and WWOX mRNA between the patients with serious illness or poorly differentiated squamous cell carcinoma and mild cases or highly differentiated squamous cell carcinoma were significantly different in the experimental group,the difference is statistically significant (P<0.05)Meanwhile,the FHIT and WWOX mRNA expression had statistical association with the clinical stage and histological type(r=-0.731,P=0.000;r=-0.816,P=0.000;r=-0.626,P=0.000;r=-0.536,P=0.001).The MDR1 mRNA expression was different between poorly and highly differentiated squamous cell carcinoma (P=0.021),which was statistical associated with the histological type (r=-0.697,P<0.001).(2)The degrees of FHIT and WWOX promoter methylation in the experimental group was higher than those in the control group,the difference was statistically significant (P<0.05);Also,the expression of FHIT and WWOX mRNA were closely related to the degree of promoter methylation(r=-0.689,P=0.000;r=-0.594,P=0.000).(3) In the experimental group,there were 39 cases (43.8%) of LOH in the FHIT gene,and 42 cases (47.2%)of the WWOX genes were significantly higher than those in the control group (4.9% and 3.3%),the difference was statistically significant (P<0.05).The FHIT and WWOX gene mRNA were negatively correlated with the loss of heterozygosity(r=-0.239,P=0.049;r=-0.364,P=0.013).Conclusion Promoter methylation is the main reason for the down-regulation of FHIT gene and WWOX gene expression in nasopharyngeal carcinoma patients,which may be the main reason for the occurrence and development of nasopharyngeal carcinoma.The higher expression of MDR 1 mRNA is statistical association with the histological type.

To improve the effect of teaching practice of blood cell morphology,typical cases of peripheral blood erythrocyte,granulocyte and platelet were chosen,case show and problem-based teaching way were applied to encourage students to find information of autonomous learning.Then teacher organized the class discussion,and the information was analyzed and summarized.In the implementation of the case teaching,we should pay attention to improving students' learning initiative.At the same time,teachers need to have knowledge,comprehensive ability and ability in organization and leadership,to promote students' active participation in discussion in the teaching,pay attention to the information feedback,and improve the teaching details

Objective To explore the effects of low density lipoprotein(LDL) containing immune complexes(IC) on the mRNA expression of matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-1(TIMP-1) in U937 cells.Methods U937 cells were incubated with native LDL,oxidized LDL or LDL-IC,respectively.mRNA expression of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in U937 cells were measured by reverse transcriptase polymerase chain reaction(PT-PCR).Results Normal U937 cells hardly expressed MMP-1,but highly expressed TIMP-1.N-LDL had no effect on the expression of MMP-1 and TIMP-1 mRNA.Ox-LDL can induce slightly expression of MMP-1 and markedly down-regulate expression of TIMP-1.Similarly,nature and oxidized LDL-IC had significantly more effect on the mRNA expressions of MMP-1 and TIMP-1 than that of control(P

Objective To study the value of hysteroscopy in the diagnosis and treatment of infertility caused by intrauterine lesions. Methods Hysteroscopic examinations and operations were performed in 82 cases of infertility caused by intrauterine lesions. Results No abnormal findings were found in 4 cases,while the other 78 cases of intrauterine abnormity comprised 23 cases of endometrial polyp, 29 cases of adhesive uteritis,18 cases of submucous myoma or adenomyosis, and 6 cases of uterine deformity. After hysteroscopic surgery and related treatment, the postoperative pregnancy rate reached 62 32% (43 of 69). Conclusions Hysteroscopy may be effectively used in the diagnosis and treatment of infertility caused by intrauterine factors.