Objective:To explore the static and dynamic improvement effects of dual nerve transfer (hypoglossal nerve-temporal bone trunk end-to-side anastomosis, masseteric nerve-buccal branch end-to-end anastomosis) in the treatment of peripheral facial paralysis after acoustic neuroma resection surgery.Methods:A retrospective analysis was conducted on the clinical data of patients with peripheral facial paralysis after acoustic neuroma surgery who underwent dual nerve transfer surgery in the Department of Plastic Surgery of China-Japan Friendship Hospital from January 2022 to January 2024. The clinical data included standardized photographs and videos before and 1 year after surgery. House-Brackmann (H-B) grading of facial paralysis (Grade Ⅰ to Ⅵ, higher grade indicates worse facial nerve function), electronic facial assessment by computer evaluation (eFACE) (0 to 100 points, higher scores indicate better facial function), facial disability index-physical (FDIP) (-25 to 100 points, higher scores indicate better facial function), facial clinimetric evaluation (FaCE) (0 to 100 points, higher scores indicate better facial function) were used as the effectiveness evaluation indicator, and safety data were collected through patient reports. The measurement data of normal distribution were expressed as Mean±SD and the paired t-test was used. The grade data were analyzed using the Wilcoxon signed-rank test. When conducting correlation tests, the Pearson correlation test was used for normally distributed data. Results:A total of 20 patients were enrolled, including 7 males and 13 females, with an average age of (33.4 ± 6.5) years (22 to 44 years). The duration of facial paralysis was (10.3 ± 4.5) months (1 to 20 months). Before the operation, the H-B grades of all patients were grade Ⅵ. At the 1-year follow-up after the operation, all patients showed significant improvement in facial static tension and smile movement. The H-B grades of the affected sides of all patients improved. Among them, 18 cases decreased from grade Ⅵ to grade Ⅲ, and 2 cases decreased from grade Ⅵ to grade Ⅱ. The differences in H-B grades of the affected sides before and after the operation were statistically significant ( P < 0.01). Compared with those before the operation, the static eFACE scores [(64.55±12.62) points vs. (84.25±9.08) points], dynamic eFACE scores [(34.85±9.31) points vs. (68.70±5.36) points], FDIP scores [(28.50±8.13) points vs. (59.50±5.36) points], and FaCE scores [(23.33±9.23) points vs. (61.92±9.65) points] of the affected sides significantly increased, and the differences were statistically significant (all P<0.01). Correlation analysis showed that there was a negative correlation between age and the postoperative eFACE static score ( r=-0.61, P < 0.01). Five patients complained of mild chewing weakness and bilateral chewing asymmetry, which did not affect their daily lives, no patients had tongue atrophy, tongue extension weakness, speech or swallowing disorders. Conclusion:Dual nerve transfer surgery provides both facial static tension and smile dynamics, and has remarkable clinical efficacy in treating peripheral facial paralysis after acoustic neuroma surgery.

Accurate prediction of drug-target interactions (DTIs) plays a pivotal role in drug discovery, facilitating optimization of lead compounds, drug repurposing and elucidation of drug side effects. However, traditional DTI prediction methods are often limited by incomplete biological data and insufficient representation of protein features. In this study, we proposed KG-CNNDTI, a novel knowledge graph-enhanced framework for DTI prediction, which integrates heterogeneous biological information to improve model generalizability and predictive performance. The proposed model utilized protein embeddings derived from a biomedical knowledge graph via the Node2Vec algorithm, which were further enriched with contextualized sequence representations obtained from ProteinBERT. For compound representation, multiple molecular fingerprint schemes alongside the Uni-Mol pre-trained model were evaluated. The fused representations served as inputs to both classical machine learning models and a convolutional neural network-based predictor. Experimental evaluations across benchmark datasets demonstrated that KG-CNNDTI achieved superior performance compared to state-of-the-art methods, particularly in terms of Precision, Recall, F1-Score and area under the precision-recall curve (AUPR). Ablation analysis highlighted the substantial contribution of knowledge graph-derived features. Moreover, KG-CNNDTI was employed for virtual screening of natural products against Alzheimer's disease, resulting in 40 candidate compounds. 5 were supported by literature evidence, among which 3 were further validated in vitro assays.
Alzheimer Disease/drug therapy* ; Biological Products/therapeutic use* ; Humans ; Neural Networks, Computer ; Machine Learning ; Drug Discovery/methods* ; Algorithms ; Drug Evaluation, Preclinical/methods*

AIM:This study aimed to confirm the glycolytic inhibitory activity of 3-bromopyruvic acid(3BP)and to assess whether this inhibition could ameliorate hypoxia-induced pulmonary hypertension in rats.METHODS:PAH model rats were generated from normal SD rats via exposure to normal pressure and hypoxia.Intervention groups I and II(6 rats per group)were then intraperitoneally injected with 3BP(15 mg/kg),and the normal and hypoxia groups(6 rats per group)were given the same amount of normal saline for a total of 21 d.The average pulmonary artery pressure of the rats in each group was measured via right heart catheterisation,and hilar tissue measurements.The right ventricle(RV),left ventricle,and interventricular septum(LV+S)were weighed,and the ratio of RV/(LV+S)was calculated as an index of right ventricular hypertrophy.Right lower lung tissues were fixed in 4%paraformaldehyde-PBS buffer,sec-tioned in conventional paraffin(5 μm thick),stained with HE and Masson,photographed under a microscope.Then the thickness ratio of the tunica media and the area ratio of collagen fibres were calculated.The expression of pyruvate kinase isozyme type M2(PKM2),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),and pyruvate de-hydrogenase(PDH)proteins in the hilar tissues of each group were detected by western blot,whereas interleukin-1β(IL-1β)and IL-18 contents were detected using ELISA,and lactic acid content was detected using a lactic acid kit.RE-SULTS:The results showed that 3-brpa effectively inhibited glycolysis and significantly improved hypoxia-induced pulmo-nary hypertension in rats.Compared with the hypoxia group,in intervention group II,PKM2 expression was decreased(P<0.05),PDH expression increased significantly(P<0.01),and NLRP3 expression was decreased(P<0.05).The IL-18 and IL-1β contents decreased(P<0.05 or P<0.01,respectively).Pulmonary hemodynamic indexes showed that the pro-portion of the right ventricle and the mean pressure of the pulmonary artery decreased(P<0.05 or P<0.01,respectively).The HE and Masson staining results showed that the thickness ratio of the tunica media and the area ratio of collagen fibres decreased significantly(P<0.01).Lactic acid content was significantly decreased(P<0.01).CONCLUSION:This study showed that 3BP can inhibit glycolysis and alleviate hypoxia-induced pulmonary hypertension in rats.

Objective To address the challenges of accurately capturing critical features in small-sample diabetic retinopathy(DR)recognition models in real-world applications,and the overly smooth distribution of true and false feature coefficients,we propose an enhanced small-sample DR recognition method based on an improved capsule network.Methods Firstly,the method enhances image feature representation by removing redundant boundary information and employing discrete wavelet transform based on the Haar wavelet function,thereby high-lighting critical pathological features.Secondly,the convolutional layer of the capsule network is optimized through a multi-branch architecture to extract multi-scale features from retinal images,while incorporating a convolutional block attention module that is subsequently fed into the capsule layer.Finally,the sigmoid function replaces the soft-max function in dynamic routing,thereby improving the model's robustness.Result The enhanced neural network model achieved an accuracy of 98.62%on the Kaggle public dataset following a rigorous selection and preprocessing procedure.Conclusion The enhanced capsule network demonstrated superior precision in identifying diabetic reti-nopathy within small sample sizes compared to other state-of-the-art algorithms currently available.

[Abtract]Nursing safety management is crucial to patient health outcomes,and real-time monitoring and early warning systems play a vital role in enhancing nursing safety and reducing medical incidents.Wearable flexible devices enable non-invasive,continuous monitoring of various physiological parameters and provide timely alerts.These devices not only improve the efficiency and quality of nursing safety management but also offer patients more convenient,accurate,and personalized care services.This article provides a comprehensive review of the application of wearable flexible devices in nursing safety management,examining their application scenarios,advantages,and challenges,and offering insights to facilitate the further integration of this technology into nursing safety practices.

[Abtract]Nursing safety management is crucial to patient health outcomes,and real-time monitoring and early warning systems play a vital role in enhancing nursing safety and reducing medical incidents.Wearable flexible devices enable non-invasive,continuous monitoring of various physiological parameters and provide timely alerts.These devices not only improve the efficiency and quality of nursing safety management but also offer patients more convenient,accurate,and personalized care services.This article provides a comprehensive review of the application of wearable flexible devices in nursing safety management,examining their application scenarios,advantages,and challenges,and offering insights to facilitate the further integration of this technology into nursing safety practices.

AIM:This study aimed to confirm the glycolytic inhibitory activity of 3-bromopyruvic acid(3BP)and to assess whether this inhibition could ameliorate hypoxia-induced pulmonary hypertension in rats.METHODS:PAH model rats were generated from normal SD rats via exposure to normal pressure and hypoxia.Intervention groups I and II(6 rats per group)were then intraperitoneally injected with 3BP(15 mg/kg),and the normal and hypoxia groups(6 rats per group)were given the same amount of normal saline for a total of 21 d.The average pulmonary artery pressure of the rats in each group was measured via right heart catheterisation,and hilar tissue measurements.The right ventricle(RV),left ventricle,and interventricular septum(LV+S)were weighed,and the ratio of RV/(LV+S)was calculated as an index of right ventricular hypertrophy.Right lower lung tissues were fixed in 4%paraformaldehyde-PBS buffer,sec-tioned in conventional paraffin(5 μm thick),stained with HE and Masson,photographed under a microscope.Then the thickness ratio of the tunica media and the area ratio of collagen fibres were calculated.The expression of pyruvate kinase isozyme type M2(PKM2),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),and pyruvate de-hydrogenase(PDH)proteins in the hilar tissues of each group were detected by western blot,whereas interleukin-1β(IL-1β)and IL-18 contents were detected using ELISA,and lactic acid content was detected using a lactic acid kit.RE-SULTS:The results showed that 3-brpa effectively inhibited glycolysis and significantly improved hypoxia-induced pulmo-nary hypertension in rats.Compared with the hypoxia group,in intervention group II,PKM2 expression was decreased(P<0.05),PDH expression increased significantly(P<0.01),and NLRP3 expression was decreased(P<0.05).The IL-18 and IL-1β contents decreased(P<0.05 or P<0.01,respectively).Pulmonary hemodynamic indexes showed that the pro-portion of the right ventricle and the mean pressure of the pulmonary artery decreased(P<0.05 or P<0.01,respectively).The HE and Masson staining results showed that the thickness ratio of the tunica media and the area ratio of collagen fibres decreased significantly(P<0.01).Lactic acid content was significantly decreased(P<0.01).CONCLUSION:This study showed that 3BP can inhibit glycolysis and alleviate hypoxia-induced pulmonary hypertension in rats.

Objective To address the challenges of accurately capturing critical features in small-sample diabetic retinopathy(DR)recognition models in real-world applications,and the overly smooth distribution of true and false feature coefficients,we propose an enhanced small-sample DR recognition method based on an improved capsule network.Methods Firstly,the method enhances image feature representation by removing redundant boundary information and employing discrete wavelet transform based on the Haar wavelet function,thereby high-lighting critical pathological features.Secondly,the convolutional layer of the capsule network is optimized through a multi-branch architecture to extract multi-scale features from retinal images,while incorporating a convolutional block attention module that is subsequently fed into the capsule layer.Finally,the sigmoid function replaces the soft-max function in dynamic routing,thereby improving the model's robustness.Result The enhanced neural network model achieved an accuracy of 98.62%on the Kaggle public dataset following a rigorous selection and preprocessing procedure.Conclusion The enhanced capsule network demonstrated superior precision in identifying diabetic reti-nopathy within small sample sizes compared to other state-of-the-art algorithms currently available.

Objective:To explore the static and dynamic improvement effects of dual nerve transfer (hypoglossal nerve-temporal bone trunk end-to-side anastomosis, masseteric nerve-buccal branch end-to-end anastomosis) in the treatment of peripheral facial paralysis after acoustic neuroma resection surgery.Methods:A retrospective analysis was conducted on the clinical data of patients with peripheral facial paralysis after acoustic neuroma surgery who underwent dual nerve transfer surgery in the Department of Plastic Surgery of China-Japan Friendship Hospital from January 2022 to January 2024. The clinical data included standardized photographs and videos before and 1 year after surgery. House-Brackmann (H-B) grading of facial paralysis (Grade Ⅰ to Ⅵ, higher grade indicates worse facial nerve function), electronic facial assessment by computer evaluation (eFACE) (0 to 100 points, higher scores indicate better facial function), facial disability index-physical (FDIP) (-25 to 100 points, higher scores indicate better facial function), facial clinimetric evaluation (FaCE) (0 to 100 points, higher scores indicate better facial function) were used as the effectiveness evaluation indicator, and safety data were collected through patient reports. The measurement data of normal distribution were expressed as Mean±SD and the paired t-test was used. The grade data were analyzed using the Wilcoxon signed-rank test. When conducting correlation tests, the Pearson correlation test was used for normally distributed data. Results:A total of 20 patients were enrolled, including 7 males and 13 females, with an average age of (33.4 ± 6.5) years (22 to 44 years). The duration of facial paralysis was (10.3 ± 4.5) months (1 to 20 months). Before the operation, the H-B grades of all patients were grade Ⅵ. At the 1-year follow-up after the operation, all patients showed significant improvement in facial static tension and smile movement. The H-B grades of the affected sides of all patients improved. Among them, 18 cases decreased from grade Ⅵ to grade Ⅲ, and 2 cases decreased from grade Ⅵ to grade Ⅱ. The differences in H-B grades of the affected sides before and after the operation were statistically significant ( P < 0.01). Compared with those before the operation, the static eFACE scores [(64.55±12.62) points vs. (84.25±9.08) points], dynamic eFACE scores [(34.85±9.31) points vs. (68.70±5.36) points], FDIP scores [(28.50±8.13) points vs. (59.50±5.36) points], and FaCE scores [(23.33±9.23) points vs. (61.92±9.65) points] of the affected sides significantly increased, and the differences were statistically significant (all P<0.01). Correlation analysis showed that there was a negative correlation between age and the postoperative eFACE static score ( r=-0.61, P < 0.01). Five patients complained of mild chewing weakness and bilateral chewing asymmetry, which did not affect their daily lives, no patients had tongue atrophy, tongue extension weakness, speech or swallowing disorders. Conclusion:Dual nerve transfer surgery provides both facial static tension and smile dynamics, and has remarkable clinical efficacy in treating peripheral facial paralysis after acoustic neuroma surgery.