Objective To evaluate the therapeutic effect of antimicrobial photodynamic therapy(aPDT)combined with subgingival scaling and root planing(SRP)on chronic periodontitis.Methods A total of 198 patients with chronic periodontitis who were diagnosed and treated in the Department of Stomatology of the hospital from August 2023 to April 2024 were selected as the study subjects.A randomized block design was used to divide the patients into a control group(n=98)and an experimental group(n=98).The control group received oral education and SRP treatment,while the experimental group received oral education and aPDT+SRP treatment.The depth of periodontal pocket probing(PD),positive rate of bleeding probing(BOP),bleeding index(BI),and plaque index(PLI)were compared of patients before and after 6 weeks of treatment,and the levels of tumor necrosis factor-α(TNF-α)and IL-6 in gingival crevicular fluid were meas-ured.Results After treatment,the proportion of patients with PLI grade 0 and 1 in the experimental group was higher than the control group(x2=30.640,P＜0.001).The PD of the experimental group was lower than that of the control group(t=9.420,P＜0.001).The AL of the experimental group was lower than that of the control group(t=4.495,P＜0.001).The proportion of patients with grade 0 and grade 1 of BI index in the experimental group was higher(x2=48.839,P＜0.001).The average levels of IL-6 and TNF-α in the ex-perimental group were lower than those in the control group(P＜0.001).Conclusion SRP+aPDT therapy not only effectively improves periodontal clinical indicators in patients with chronic periodontitis,but also sig-nificantly reduces the levels of local inflammatory factors,which is worthy of promotion and application.

Objective To investigate the angiogenesis effects of Salvia miltiorrhiza in heterotopically grafted frozen-thawed mouse ovaries. Methods The ovaries thawed after cryopreservation were xenografted into the donated kidney capsules of 8- to 12-week adult male mice. The mice were divided into two groups, saline and Salvia miltiorrhiza groups, the mice either in the saline or in Salvia miltiorrhiza groups were administered I.p. Daily either saline(0.5ml) or Salvia miltiorrhiza(0.5g)respectively, from the day prior transplantation. The two groups were sacrificed 1 day,2 days and 7 days after transplantation respectively, the grafts from thawed,1 day,2 days,7 days were removed for follicle counting, immunohistochemical studying and detecting of the mRNA expression of vascular endothelial growth factor(VEGF). Results The number of follicles and survival rates in grafts after transplantation of Salvia miltiorrhiza group were more than that of saline group (P<0.05);the expression of VEGF increased after transplantation,peaked on day 7,there was no difference between the two groups (P>0.05);the apoptosis index of Salvia miltiorrhiza group was less than that of saline group (P<0.05), the mRNA expression of VEGF188 and VEGF164 of Salvia miltiorrhiza group was more than that of saline group on 48 hours after transplantation(P<0.05). Conclusion Salvia miltiorrhiza may provide benefits for folliculogensis and decreasing the apoptosis index of follicles. Nevertheless,a increase in the VEGF188 and VEGF164 isoform in the Salvia miltiorrhiza group may suggest the positive effect of exogenous Salvia miltiorrhiza therapy in the early stage of angiogenesis.