Objective To construct a patient-derived pancreatic tumor organoid(PDO)and evaluate its effectiveness.Methods We collected fresh surgical specimens from pancreatic cancer patients for PDO culture and compared the pathological and genetic characteristics of the PDO model with those of primary tumors.The PDO model was used to evaluate the efficacy of clinical chemotherapy drugs,and the effectiveness of the model was assessed.Results A PDO model of pancreatic cancer was successfully established.Histomorphological analysis indicated that the PDO model maintained the basic pathological characteristics of the primary tumor.Whole-exon sequencing showed that both the organoids and original tumor tissue remained consistent in their gene mutation type and characteristics.Drug screening tests revealed that the PDO model had good sensitivity to gemcitabine and irinotecan.Conclusions A pancreatic cancer PDO was successfully constructed that reflected the histological and genetic characteristics of the original tumor.The model was shown to be effective for drug sensitivity experiments in vitro and is expected to have implications for precision medicine assays.

Objective: To observe the parameters of the results of suppression head impulse paradigm (SHIMP) in healthy adults, and to provide reference for evaluating vestibular oculomotor reflex function in patients with peripheral vertigo. Methods: Fifty healthy adults, 22 males and 28 females, aged from 23-65 years, with an average age of (38.5±11.6) years, were recruited from January to March 2018. Parameters provided by the video head pulse software included the gains, the latency and the peak velocity of saccades, and comparison was made with head impulse paradigm (HIMP). Results: All subjects were elicited anti-compensatory saccades in SHIMP. The normal values of left and right gains were 1.02 and 1.10 in HIMP, and 0.93 and 1.01 in SHIMP respectively. The left and right saccades latency were (201.1± 50.8)ms and (187.0± 42.9)ms, and the peak saccadic velocity were (302.7±58.5)°/s and (291.5±46.5)°/s in SHIMP; there were small but significant difference between two sides about gains in HIMP and SHIMP, as well as latency in SHIMP(P<0.05); there were small but significant difference between HIMP and SHIMP about gains in ipsilateral(P<0.01); there were no significant difference between two sides about peak saccadic velocity in SHIMP(P>0.05). Conclusions SHIMP can be used for the examination of vestibular oculomotor reflex function in adult population. It is easy to be operated and is convenient for clinical application. Combined with head pulse test, the function of the semicircular canal can be evaluated together.

Objective To analyze and investigate the diagnostic value of strain imaging elastosonography in stationary phase plasma cell mastitis.Methods Retrospective analysis was made on the plasma cell mastitis patients,who sought medical care in the hospital from Jan 2014 to Feb 2016 and were later confirmed by pathological examination.Eighteen patients with stationary phase plasma cell mastitis were selected for analysis of the features of conventional uhrasonographic images,the BI-RADS classification and the features of elastosonography images.Results In spite of the fact that more features of tumor and higher grade of classification might be manifested in conventional ultrasonography,the scores of elastosonography were mostly 1-2,indicating that they might be benign tumors.Conclusion For stationary phase plasma cell mastitis with just breast tumor,conventional ultrasonography coupled with elastosonography could significantly increase diagnostic accuracy of stationary phase plasma cell mastitis.

Objective To explore whether strain elastography could improve the classification diagnosis of US BI -RADS 4A breast pathologic lesion .Methods Eighty-seven breast foci of the 75 patients diagnosed with the two-dimensional color Doppler ultra-sonography as BI-RADS 4A were selected for the study , and then, strain elastography was performed .The modified 5-point method was used for further evaluation , and BI-RADS classification was performed again with the help of the two-dimensional color Doppler ultra-sonography.The cases with the scores of ≥4 were upgraded as the 4B-C grade, the cases with the scores of =3 remained in the same grade, and the cases with the scores of <3 were downgraded to be the third grade .The results were compared with the pathological re-sults.Results Of the 87 breast lesions, 6 were evaluated to have elasticity scores of ≥4, 11 were assessed to have elasticity scores of=3, and 70 were assessed to have elasticity scores of <3.There were 8 malignant tumors confirmed by pathological examination in the 87 breast lesions, of which 8 lesions had elasticity scores of ≥4, 3 lesions had elasticity scores of =3, and 2 lesions had elasticity scores of <3.With the elasticity scores of ≥3 and <3 as the standards for respectively malignant and benign tumors , the sensitivity, specificity and accuracy of the method for detecting malignant tumors were respectively 75%, 86.1% and 85.1%, and the negative predictive value (NPV) was 97.1%, which was superior to that of conventional ultrasonography (P<0.05).With further classification of BI-RADS in combined use of elastography , the malignancy rate in the benign group was reduced to 2.8%and the malignancy rate in the malignant group was increased to 35.3%, and significant differences could be noted , when compared with those previous figures ( P<0.05).Conclusion Elastography could effectively promote the classification diagnostic accuracy of BI -RADS 4A breast pathologic lesion
and could considerably reduce the rate of biopsy before surgery .With this reason , it promised good clinical application .

OBJECTIVE： To establish a HPLC assay for the determination of hydroxycamptothecinum and camptothecinum.METHODS： The concentrations of hydroxycamptothecinum and camptothecinum were assayed on a C18 column with a mobile phase consisting of methanol－ water(45∶ 55) at a flow rate of 1.0 ml/min,and detected at 266nm.RESULTS： The mean recovery of hydroxycamptothecinum was 100.2％ ,the 2,4,8 hours precision RSD were less than 1.8％ ;The mean recovery of camptothecinum was 99.7％ ,the 2,4,8 hours precision RSD were less than 2.0％ .CONCLUSION： The HPLC method for determination of hydroxycamptothecinum and camptothecinum was rapid,sensitive and accurate.

This article describes the application of ABC analysis Procedure and the corresponding card in quantitatively managing drug stock in hospital pharmacy. It will play an improtant role in improving rational drug stock,planned purchase, ensuring the quality of drugs, and raising the economic benefit.