Introduction: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin secretion or action. The global prevalence of DM is increasing rapidly, threatening public health and quality of life. In Mongolia, the incidence of type 2 diabetes mellitus (T2DM) has shown a continuous upward trend, highlighting the need for effective plant-derived formulations with glucose-lowering potential.
This study aimed to evaluate the antihyperglycemic effect of composite formulations containing Lagerstroemia speciosa L. (Banaba), Helianthus tuberosus L., and Dasiphora fruticosa L. in alloxan-induced diabetic rats. Methods: T2DM was induced in Wistar rats by intraperitoneal injection of alloxan monohydrate (170 mg/kg). The rats were divided into seven groups: Group 1: healthy control, Group 2: diabetic control, Group 7: metformin-treated (40 mg/200 g), and four treatment groups (Groups 3-6) receiving different combinations of Banaba, Jerusalem artichoke, and Shrubby cinquefoil extracts for 19 consecutive days. Blood glucose levels were measured daily using a GluNeo® Lite glucometer.
After 19 days, animals in Group and Group 6 exhibited significant reductions in blood glucose levels compared with the diabetic (Group 2) and metformin-treated (Group 7) controls (p<0.01). Group 5 demonstrated the greatest hypoglycemic effect, reducing glucose levels by 43.4% relative to the diabetic control group (Group 2). Conclusion Composite formulations containing Banaba, Helianthus tuberosus L., and Dasiphora fruticosa L. exhibited significant antihyperglycemic activity in alloxan-induced diabetic rats. The formulation with Banaba (24 mg) + Helianthus tuberosus L. (200 mg) + Dasiphora fruticosa L. (100 mg) showed the most potent glucose-lowering effect, comparable to metformin, indicating its potential as a promising herbal formulation for T2DM management.

Introduction: Inula helenium L. is a medicinal plant widely used in traditional Mongolian and Tibetan medicine. Its roots and rhizomes contain active compounds, including sesquiterpene lactones (alantolactone and isoalantolactone), inulin, flavonoids, and essential oils, with anti-inflammatory, antimicrobial, and immunomodulatory properties. This study evaluates the quality of Inula helenium L. roots and quantifies their key active compounds in accordance with pharmacopoeial standards. Methods: The roots of Inula helenium L. cultivated in the Medicinal Plant Botanical Garden of Monos Group were used as raw material. Physicochemical parameters, including moisture, total ash, acid-insoluble ash, and extractive values, were determined according to pharmacopoeial methods. Qualitative analysis was carried out using color reactions for polysaccharides and thin-layer chromatography for the identification of sesquiterpene lactones. Quantitative analysis was performed using spectrophotometry for total polysaccharides and high-performance liquid chromatography (HPLC) for the determination of alantolactone and isoalantolactone. Conclusion The physicochemical parameters of Inula helenium L. root were standardized, with total inulin content determined as 47.13±0.45%, alantolactone as 1.41±0.02%, and isoalantolactone as 1.33±0.02%. Analytical methods for inulin, alantolactone, and isoalantolactone were validated according to ICH Q2(R2) guidelines, confirming the possibility of updating the MNS standard or developing a manufacturer’s pharmacopoeial monograph based on these validated methods.