Background: Dysregulation of inflammation underpins chronic cardiovascular, metabolic, neurodegenerative, and neoplastic conditions. Traditional medicine has long utilized medicinal plants to alleviate and treat systemic inflammation. Specifically, Rhodiola rosea L. and Rhodiola quadrifida (Pall.) Fisch. & C.A.Mey. possess anti-inflammatory, antitumor, antioxidant, and anti-angiogenic properties. Saposhnikovia divaricata (Turcz.) Schischk. has been shown to exert anti-inflammatory effects, support musculoskeletal function, and enhance tissue regeneration. Furthermore, Salsola laricifolia Litv. has been studied for its antioxidant, immunomodulatory, and anti-inflammatory activities. Although the individual anti-inflammatory, antioxidant, and antitumor activities of these plants are established, research evaluating the combined effect of their combined extract remains scarce, providing the rationale for this study. Aim: To evaluate the in vitro effects of a combined extract derived from these medicinal plants on the expression of selected pro-inflammatory cytokines. Materials and Methods: A dry combined extract consisting of Rhodiola rosea, Rhodiola quadrifida, Salsola laricifolia, and Saposhnikovia divaricata was prepared at the Chemistry and Technology Sector of the Institute of Pharmaceutical Research, Monos Group. An in vitro inflammation model was established using murine RAW264.7 macrophage-like cells stimulated with lipopolysaccharide (LPS, 100 ng/mL). Cell viability was assessed using the CCK-8 assay, while the output of pro-inflammatory cytokines TNF-α and IL-6 was measured via Enzyme-Linked Immunosorbent Assay (ELISA). Statistical analysis was performed using one-way ANOVA. Result: The combined plant extract demonstrated no cytotoxic effects on RAW264.7 cells across the tested concentration range. In LPS-stimulated RAW264.7 macrophages, the composite extract dose-dependently and significantly suppressed the production of the pro-inflammatory cytokines TNF-α (p<0.001) and IL-6 (p<0.0001). Conclusion 1. The combined extract of Saposhnikovia divaricata, Rhodiola rosea, Rhodiola quadrifida, and Salsola laricifolia exhibited no cytotoxicity in vitro within the range of 50–400 µg/mL. 2. The combined herbal extract significantly suppressed the production of pro-inflammatory cytokines TNF-α (p<0.01) and IL-6 (p<0.001) in LPS-induced RAW264.7 macrophages in a concentration-dependent manner.

Background: Medicinal herbs have been used in traditional medicine to treat systemic inflammatory disease for many years. For instance, Rhodiola rosea L extracts were used to enhance behavioural stresses for improving fatigue and depression. Gallic acid, found in Rhodiola rosea L and Rhodiola quidrifida is, a natural polyphenol, exhibits multiple therapeutic activities, including anti-inflammatory, anti-cancer, antioxidant, and anti-angiogenic effects. Saposhnikovia divaricate (its ethanol extract mainly) notified to support function of musculoskeletal tissue and to enhance tissue regeneration by its anti-inflammatory effect. The Salsola laricifolia L has been studied for its strong antioxidant activity, improve immune function, boost energy, and exhibit anti-inflammatory effects. This study allowed us to screen anti-inflammatory effects of medicinal plants and future therapeutic possibility. Aim: To study the content of phenolic compounds (gallic acid) in the composition of complex products of plants widely used in Mongolian traditional medicine, which have been found to have biological activity of the extracts Materials and Methods: The herbal extract was extracted from Rhodiola rosea L, Saposhnikovia divaricata (Tuscz) Schischk, Rhodiola quidrifida Pall.Fisch, Salsola laricifolia Turcz.ex Litv in laboratory of the Drug Research Institute of Monos Group, Mongolia. We used solvents for HPLC grade was used to identify the bioactive components that Gallic acid in the compound plant extract. : Results: The bioactive compounds in each of the Rhodiola rosea L, Rhodiola quadrifida Pall.Fisch and four medicinal plant extracts were identified using HPLC, confirming the presence of Gallic acid. Conclusion Our research results showed that the quantitative content of Gallic acid in the composite plant extract was high, at 1.02%.