Objective: To evaluate the clinical efficacy and safety of injectable platelet-rich fibrin (i-PRF) in the treatment of mild to moderate female pattern hair loss (FPHL), and to analyze the related clinical factors affecting the therapeutic efficacy of i-PRF. Methods: This was a secondary analysis of a prospective, randomized controlled trial. A total of 29 FPHL patients with Sinclair grade Ⅱ-Ⅲ, who attended the outpatient clinics of Beijing Friendship Hospital, Capital Medical University and Peking University Third Hospital from March 2024 to April 2025, were enrolled. All patients received i-PRF treatment once every 4 weeks for a total of 4 sessions, with follow-up up to 24 weeks. Standardized clinical photographs were taken at baseline, week 12 and week 24. Trichoscopic examination was performed to measure the non-vellus target area hair count (TAHC) and hair diameter (HD) in the target area, and all adverse events were recorded. The Mann-Whitney U test was used to compare the intergroup differences in efficacy for categorical influencing factors; Spearman rank correlation analysis was employed to assess the correlation between continuous influencing factors and efficacy; and multiple linear regression (enter method) was performed to identify independent influencing factors of efficacy. Results: A total of 27 patients completed the trial. At week 12, TAHC significantly increased from 112 (105, 120) hairs/cm at baseline to 128 (110, 150) hairs/cm , with a median increase of 12 (5, 28) hairs/cm and a median growth rate of 10.5 (4.2, 24.5)% (P=0.045); hair diameter significantly increased from 42.5 (39.8, 45.2) μm to 52.0 (47.0, 58.0) μm, with a median increase of 7.5 (3.0, 12.5) μm and a median growth rate of 18.0 (7.5, 29.0)% (P<0.001). At week 24, TAHC further significantly increased to 141 (125, 158) hairs/cm (P<0.001), with a median growth rate of 24.8 (18.5, 32.0)%; hair diameter continued to improve to 55.0 (50.0, 61.0) μm (P=0.008), with a median growth rate of 21.5 (16.0, 27.0)%. The overall incidence of adverse events was 10.34% (3/29), mainly presenting as mild scalp pruritus and transient headache, without severe adverse effects. Multiple linear regression analysis revealed that baseline TAHC was an independent influencing factor for i-PRF efficacy (P<0.05), while age, BMI, disease duration, Sinclair grade, family history of AGA, and baseline HD showed no significant independent effect on efficacy in this model (P>0.05). Conclusion: This preliminary study indicates that i-PRF can improve hair density and hair shaft diameter in patients with FPHL with a favorable safety profile. Patients with FPHL and lower baseline TAHC may achieve greater clinical improvement.

Objective To investigate the clinical efficacy and safety of modified single-port thoracoscopic sympathectomy combined with disconnection ventilation in the treatment of primary palmar hyperhidrosis(PPH).Methods The perioperative indexes,clinical efficacy and postoperative complications of 20 patients with PPH who underwent modified single-port thoracoscopic sympathectomy combined with disconnection ventilation in Wuming Hospital of Guangxi Medical University from June 2020 to July 2024 were retrospectively analyzed.Results All patients successfully completed the operation without serious complications and death.The average operation time was(56.15±16.00)min,and the average intraoperative blood loss was(3.35±1.39)ml.The treatment effective rate was 100%,the average postoperative hospitalization time was(2.15±0.86)days,and the average hospitalization cost was(9485.56±1601.77)yuan.During the follow-up period of 1 to 45 months,there were no recurrent cases and no obvious cases of compensatory hyperhidrosis.Conclusion The modified single-port thoracoscopic sympathectomy combined with disconnection ventilation has good efficacy and safety in the treatment of PPH.

Objective:To explore the mechanism of Yiqi Jiedu Huayu Recipe(YQJDHY)on ulcerative colitis model rats based on the negative regulation of JAK2/STAT3 pathway by SOCS.Methods:The rats with ulcerative colitis were cloned by TNBS/ethanol method,and randomly divided into normal group,model group,SASP group,YQJDHY high-dose,medium-dose and low-dose groups.Serum IL-21 and IL-22 were detected by ELISA.Real-time quantitative polymerase chain assay(RT-PCR)was used to detect IL-23,IL-6,IL-17A,cytokine signaling inhibitor 1(SOCS1),SOCS3,tyrosine protein kinase 2(JAK2),transcription activa-tor 3(STAT3),retinoic acid-associated orphan receptorγ antibody(RORγt)mRNA expressions.Results:Pathological examination of rats in model group showed obvious inflammation and ulceration,indicating successful modeling.Compared with normal group,IL-21 and IL-22 contents in model group were significantly increased(P<0.05);the down-regulation of IL-21 and IL-22 in YQJDHY groups and SASP group were statistically different(P<0.05,P<0.01).Compared with model group,IL-23,IL-6,JAK2,STAT3 and IL-17A mRNA levels in intestinal mucosa and serum of YQJDHY groups and SASP group were significantly down-regulated(P<0.01),the ex-pressions of SOCS1 and SOCS3 mRNA in intestinal mucosa were increased in YQJDHY groups(P<0.01).The levels of RORγt mRNA in serum and intestinal mucosa of YQJDHY high-dose group and SASP group were increased(P<0.01).Compared with SASP group,the mRNA levels of JAK2 and STAT3 in YQJDHY high-dose group were increased(P<0.05).Conclusion:YQJDHY can increase the levels of SOCS1 and SOCS3 in ulcerative colitis model rats,and then inhibit JAK2/STAT3 signaling pathway,down-regulate the pro-duction of inflammatory factors,and reduce immune inflammatory response.

Objective To understand the individual dose monitoring of occupational external exposure for radiation workers in Wuhan City and analyze the dose change trend, and to provide a scientific basis for radiation protection management of radiation workers. Methods The data on the monitoring results of occupational external exposure of radiation workers in Wuhan City from 2017 to 2021 were collected through the National Personal Dose Registration System, and the individual dose levels of different years, different occupational categories, and different levels of hospitals were analyzed. Results A total of 9 134 radiation workers were investigated, with an average annual effective dose per capita of 0.20 mSv/a. The overall personal annual effective dose from 2017 to 2021 showed a decreasing trend (P<0.001). The per capita annual effective dose in medical applications was higher than that in industrial applications (0.22 mSv vs 0.14 mSv; P<0.001). Among medical applications, diagnostic radiologists had the highest average annual effective dose (0.27 mSv), and among industrial applications, industrial irradiators had the highest average annual effective dose (0.29 mSv). The proportion of personnel with personal annual effective doses exceeding 1 mSv was higher in interventional radiology and industrial nondestructive testing (4.90% and 1.90%). The annual effective dose per capita in Class I and unrated hospitals was higher (0.35 mSv). Conclusion The average annual effective dose of radiation workers in Wuhan City has decreased year by year and has not exceeded the national standard limit (20 mSv). Radiation protection management still needs to focus on personnel with personal annual effective doses exceeding 1mSv in interventional radiology and industrial nondestructive testing, and supervision over primary healthcare institutions and industrial radiation should be strengthened.

Objective To investigate the clinical efficacy and safety of modified single-port thoracoscopic sympathectomy combined with disconnection ventilation in the treatment of primary palmar hyperhidrosis(PPH).Methods The perioperative indexes,clinical efficacy and postoperative complications of 20 patients with PPH who underwent modified single-port thoracoscopic sympathectomy combined with disconnection ventilation in Wuming Hospital of Guangxi Medical University from June 2020 to July 2024 were retrospectively analyzed.Results All patients successfully completed the operation without serious complications and death.The average operation time was(56.15±16.00)min,and the average intraoperative blood loss was(3.35±1.39)ml.The treatment effective rate was 100%,the average postoperative hospitalization time was(2.15±0.86)days,and the average hospitalization cost was(9485.56±1601.77)yuan.During the follow-up period of 1 to 45 months,there were no recurrent cases and no obvious cases of compensatory hyperhidrosis.Conclusion The modified single-port thoracoscopic sympathectomy combined with disconnection ventilation has good efficacy and safety in the treatment of PPH.

Objective:To explore the mechanism of Yiqi Jiedu Huayu Recipe(YQJDHY)on ulcerative colitis model rats based on the negative regulation of JAK2/STAT3 pathway by SOCS.Methods:The rats with ulcerative colitis were cloned by TNBS/ethanol method,and randomly divided into normal group,model group,SASP group,YQJDHY high-dose,medium-dose and low-dose groups.Serum IL-21 and IL-22 were detected by ELISA.Real-time quantitative polymerase chain assay(RT-PCR)was used to detect IL-23,IL-6,IL-17A,cytokine signaling inhibitor 1(SOCS1),SOCS3,tyrosine protein kinase 2(JAK2),transcription activa-tor 3(STAT3),retinoic acid-associated orphan receptorγ antibody(RORγt)mRNA expressions.Results:Pathological examination of rats in model group showed obvious inflammation and ulceration,indicating successful modeling.Compared with normal group,IL-21 and IL-22 contents in model group were significantly increased(P<0.05);the down-regulation of IL-21 and IL-22 in YQJDHY groups and SASP group were statistically different(P<0.05,P<0.01).Compared with model group,IL-23,IL-6,JAK2,STAT3 and IL-17A mRNA levels in intestinal mucosa and serum of YQJDHY groups and SASP group were significantly down-regulated(P<0.01),the ex-pressions of SOCS1 and SOCS3 mRNA in intestinal mucosa were increased in YQJDHY groups(P<0.01).The levels of RORγt mRNA in serum and intestinal mucosa of YQJDHY high-dose group and SASP group were increased(P<0.01).Compared with SASP group,the mRNA levels of JAK2 and STAT3 in YQJDHY high-dose group were increased(P<0.05).Conclusion:YQJDHY can increase the levels of SOCS1 and SOCS3 in ulcerative colitis model rats,and then inhibit JAK2/STAT3 signaling pathway,down-regulate the pro-duction of inflammatory factors,and reduce immune inflammatory response.

Objective:To analyze the factors affecting delayed chemotherapy in children with Burkitt lymphoma (BL) and their influence on prognosis.Methods:Retrospective cohort study. Clinical data of 591 children aged ≤18 years with BL from May 2017 to December 2022 in China Net Childhood Lymphoma (CNCL) was collected. The patients were treated according to the protocol CNCL-BL-2017. According to the clinical characteristics, therapeutic regimen was divided into group A, group B and group C .Based on whether the total chemotherapy time was delayed, patients were divided into two groups: the delayed chemotherapy group and the non-delayed chemotherapy group. Based on the total delayed time of chemotherapy, patients in group C were divided into non-delayed chemotherapy group, 1-7 days delayed group and more than 7 days delayed group. Relationships between delayed chemotherapy and gender, age, tumor lysis syndrome before chemotherapy, bone marrow involvement, disease group (B/C group), serum lactate dehydrogenase (LDH) > 4 times than normal, grade Ⅲ-Ⅳ myelosuppression after chemotherapy, minimal residual disease in the interim assessment, and severe infection (including severe pneumonia, sepsis, meningitis, chickenpox, etc.) were analyzed. Logistic analysis was used to identify the relevant factors. Kaplan-Meier method was used to analyze the patients' survival information. Log-Rank was used for comparison between groups.Results:Among 591 patients, 504 were males and 87 were females, the follow-up time was 34.8 (18.6,50.1) months. The 3-year overall survival (OS) rate was (92.5±1.1)%,and the 3-year event-free survival (EFS) rate was (90.5±1.2)%. Seventy-three (12.4%) patients were in delayed chemotherapy group and 518 (87.6%) patients were in non-delayed chemotherapy group. The reasons for chemotherapy delay included 72 cases (98.6%) of severe infection, 65 cases (89.0%) of bone marrow suppression, 35 cases (47.9%) of organ dysfunction, 22 cases (30.1%) of tumor lysis syndrome,etc. There were 7 cases of chemotherapy delay in group B, which were seen in COPADM (vincristine+cyclophosphamide+prednisone+daunorubicin+methotrexate+intrathecal injection,4 cases) and CYM (methotrexate+cytarabine+intrathecal injection,3 cases) stages. There were 66 cases of chemotherapy delay in group C, which were common in COPADM (28 cases) and CYVE 1 (low dose cytarabine+high dose cytarabine+etoposide+methotrexate, 12 cases) stages. Multinomial Logistic regression analysis showed that the age over 10 years old ( OR=0.54,95% CI 0.30-0.93), tumor lysis syndrome before chemotherapy ( OR=0.48,95% CI 0.27-0.84) and grade Ⅲ-Ⅳ myelosuppression after chemotherapy ( OR=0.55,95% CI 0.33-0.91)were independent risk factors for chemotherapy delay.The 3-year OS rate and the 3-year EFS rate of children with Burkitt lymphoma in the delayed chemotherapy group were lower than those in the non-delayed chemotherapy group ((79.4±4.9)% vs. (94.2±1.1)%, (80.2±4.8)% vs. (92.0±1.2)%,both P<0.05). The 3-year OS rate of the group C with chemotherapy delay >7 days (42 cases) was lower than that of the group with chemotherapy delay of 1-7 days (22 cases) and the non-delay group (399 cases) ((76.7±6.9)% vs. (81.8±8.2)% vs. (92.7±1.3)%, P=0.002).The 3-year OS rate of the chemotherapy delay group (9 cases) in the COP (vincristine+cyclophosphamide+prednisone) phase was lower than that of the non-chemotherapy delay group (454 cases) ((66.7±15.7)% vs. (91.3±1.4)%, P=0.005). Similarly, the 3-year OS rate of the chemotherapy delay group (11 cases) in the COPADM1 phase was lower than that of the non-chemotherapy delay group (452 cases) ((63.6±14.5)% vs. (91.5±1.3)%, P=0.001). Conclusions:The delayed chemotherapy was related to the age over 10 years old, tumor lysis syndrome before chemotherapy and grade Ⅲ-Ⅳ myelosuppression after chemotherapy in pediatric BL. There is a significant relationship between delayed chemotherapy and prognosis of BL in children.

Objective:To attain comprehensive insight into the diagnostic X-ray equipment and examination frequency in radiological diagnosis and treatment institutions in Wuhan, with the purpose of assisting the health administration department in formulating medical exposure protection strategies and efficiently allocating radiological diagnosis and treatment resources.Methods:Using the census method, questionnaires on the basic information on diagnostic X-ray equipment and the annual number of examinations filled out in 2022 by the 1 030 radiological diagnosis and treatment institutions (excluding military and armed police hospitals) were collected through the Wuhan Prevention and Treatment Information Management Platform for Occupational Diseases. To obtain the data on number and frequency of diagnostic X-ray examinations, the different types of diagnostic X-ray examinations were divided by the total number of permanent residents by the end of 2021 in Wuhan.Results:In Wuhan, 1 030 radiological diagnosis and treatment institutions had 7 062 radiation workers and 2 540 diagnostic X-ray units of various types. 37.76% of units and 75.01% of radiation workers were concentrated in tertiary hospitals. The number of diagnostic X-ray units per million population was 186.10, with the top two being 48.65 DR machines per million population and 31.21 intraoral dental machines per million population. The total number of diagnostic X-ray examinations was 11 884 582, with plain radiographs and computed tomography (CT) examinations accounting for 43.61% and 43.59% of the total, respectively. The annual frequency of examinations was 379.75 and 379.52 per 1 000 population, respectively. Radiodiagnosis and treatment resources were higher in central urban areas than that in remote urban areas.Conclusions:The development of diagnostic X-ray equipment in Wuhan was experiencing rapid growth, with potential for further expansion, and the frequency has not yet recovered to the level before the COVID-19 pandemic. The allocation of radiodiagnosis and treatment resources between central urban areas and remote urban areas needs to be coordinated and the management of medical radiation protection should be continuously strengthened, so as to promote the sustainable development of inter-regional radiodiagnosis and treatment, and ensure the health and safety of examinaed patients and indivuduals.

Objective:To survey and supervise the risk of infection control and radiation safety in the radiological diagnostic workplace for COVID-19, and provide data support for the safety protection of radiographers and related staff.Methods:4 emergency hospitals for COVID-19 including 2 makeshift hospitals, module hospital and brick pattern hospital in Hubei province were performed for testing and evaluation of imaging performance and radiological protection for the 8 new installed CT scanners and places according to the national standards of WS 519-2019 and GBZ 130-2013. The infection control safety factors such as the layout of the equipment room were monitored and investigated. Two COVID-19 designated hospitals including general hospital and infectious disease specialized hospital were selected to carry out field investigation and sampling of environmental biological samples for 4 CT rooms. Then the samples were detected for the nucleic acid of novel coronavirus. The results of radiodiagnostic workplace overall arrangement, infection prevention and the nucleic acid testing were analyzed, and the biological safety reliability and risk point were evaluated.Results:The indicators of imaging performance and radiation protection for 8 CT scanners in emergency hospitals could meet the requirements of national standards.Each of 2 makeshift hospitals had 3 CT rooms with the area of 38.8 m 2 and 4 mm Pb equivalent thickness of protective shielding. The CT rooms in module hospital and brick pattern hospital were 20.0 m 2, and 35.8 m 2 in areas, with 4 mm Pb equivalent and 3 mm Pb equivalent thickness of protection shielding, respectively. The 8 radiological diagnostic workplaces of the emergency hospitals were designed and constructed based on " three zones with two passage ways" . The result of the nucleic acid test indicated that the positive samples were found at the multiple sites such as scanning bed, internal of gantry and ground touched by patients in CT scanning room. The areas such as console panel and ground were risked of pollution by the virus infected hands and feet of radiographers. In addition, the similar positive samples were found in the areas in scanning room with no touch of patients, such as observation window and air outlet. Conclusions:8 CT scanners and rooms in 4 emergency hospitals basically meet the requirements of imaging performance and radiation protection. The disinfection of COVID-19 radiodiagnostic workplace should be standardized.

Objective:To investigate the safety and protection level of radiological treatment in the CT modules in the makeshift hosptials in Wuhan during the prevention and treatment of COVID-19 cases.Methods:The layout of the CT modules in makeshift hospitals, radiological protection facilities and personal protective equipment were investigated. Based on the national standards, the CT dose index was estimated and the radiological protection level at the CT modules were measured.Results:The layout of the CT modules in makeshift hospitals is reasonable, with well-equipped radiological protection facilities. Of 23 CT modules, 20 were up to standards with acceptability of 87.0%. The other three were unqualified each with 1 detection points having values in excess of the national standards. Which, after being modified immediately reached the national standards. In addition, CT dose index for 7 CT modules were estimated, with CTDI W within ±7.5%. Conclusions:The CT modules in Wuhan meet the requirements of radiological safety and protection during the prevention and treatment of COVID-19 cases.