Endosomes are characterized by the presence of various phosphoinositides that are essential for defining the membrane properties. However, the interplay between endosomal phosphoinositides metabolism and innate immunity is yet to be fully understood. Here, our findings highlight the evolutionary continuity of RAB-10/Rab10's involvement in regulating innate immunity. Upon infection of Caenorhabditis elegans with Pseudomonas aeruginosa, an increase in RAB-10 activity was observed in the intestine. Conversely, when RAB-10 was absent, the intestinal diacylglycerols (DAGs) decreased, and the animal's response to the pathogen was impaired. Further research revealed that UNC-16/JIP3 acts as an RAB-10 effector, facilitating the recruitment of phospholipase EGL-8 to endosomes. This leads to a decrease in endosomal phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and an elevation of DAGs, as well as the activation of the PMK-1/p38 MAPK innate immune pathway. It is noteworthy that the dimerization of UNC-16 is a prerequisite for its interaction with RAB-10(GTP) and the recruitment of EGL-8. Moreover, we ascertained that the rise in RAB-10 activity, due to infection, was attributed to the augmented expression of LET-413/Erbin, and the nuclear receptor NHR-25/NR5A1/2 was determined to be indispensable for this increase. Hence, this study illuminates the significance of endosomal PI(4,5)P2 catabolism in boosting innate immunity and outlines an NHR-25-mediated mechanism for pathogen detection in intestinal epithelia.
Animals ; Caenorhabditis elegans/genetics* ; Endosomes/immunology* ; Caenorhabditis elegans Proteins/immunology* ; Phosphatidylinositol 4,5-Diphosphate/immunology* ; Immunity, Innate ; Pseudomonas aeruginosa/immunology* ; rab GTP-Binding Proteins/genetics* ; Diglycerides/metabolism*

Objective To compare imaging features and to evaluate clinical values of aortic dissection(AD) with MRI and spiral CT.Methods MRI and spiral CT findings of AD in 18 cases confirmed by clinical or surgical pathology were analysed retrospectively.Results The sensitivity and specificity of two advanced techniques in diagnosis of AD were high.MRI and CT showed the dimension,true or false lumen,intimal flap,intimal tear,thrombus and intramural hematoma of AD,aortic dilatation or stenosis and the major aortic branches.Conclusion The two techniques are important for the suspect AD,two techniques are similar to display the features of dimension,classification,true or false lumen and aortic dilatation or stenosis,MRI plays an important role in diagnosis of AD and is better than CT in demonstrating the intimal flap,intimal tear,thrombus,intramural hematoma and the involvement of the major aortic branches,there were limitation in demonstrating calcification of intimal flap or vascular wall and emergency patients.