Background: Healthcare workers play a key role in the transmission of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings. Staphylococcus aureus is a major cause of healthcare associated infections, including surgical site infections, ventilator-associated pneumonia, and bloodstream infections.
The anterior nares serve as an important reservoir for this pathogen, facilitating its spread within healthcare environments. In recent years, the rising prevalence of antimicrobial resistance has become a major public health concern, leading to increased morbidity, mortality, and healthcare costs.
Therefore, assessing nasal carriage and antimicrobial resistance patterns among healthcare workers is essential for effective infection prevention and control. Objective: To detect Staphylococcus aureus from nasal swabs of healthcare workers and to determine its antimicrobial susceptibility profile, with a particular focus on resistant strains. Materials and Methods: This descriptive cross-sectional study included 159 healthcare workers from six private hospitals in Songino Khairkhan district. Nasal vestibule swabs were collected and cultured on 5% blood agar and CHROMagar™ MRSA. Bacterial identification was performed using conventional microbiological techniques and API identification systems. Antimicrobial susceptibility testing was conducted using the disk diffusion method and interpreted according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analysis was performed using SPSS version 24. The study protocol was submitted to and approved by the Research Ethics Subcommittee of “Ach” Medical University on November 11, 2025. Written informed consent was obtained from all participants prior to sample collection. Results: A total of 159 nasal swabs were collected from healthcare workers, from which 58 (36.5%) bacterial strains were isolated. Staphylococcus aureus was the most prevalent pathogen, accounting for 50 (86.2%) of the total isolates, representing an overall carrier rate of 31.4% (50/159) among the participants. Other identified species included Enterococcus spp. (n=5, 8.6%), Streptococcus viridans (n=2, 3.4%), and Klebsiella pneumoniae (n=1, 1.7%). Antimicrobial susceptibility testing revealed that 76% (n=38) of the S. aureus isolates were Methicillin-Resistant S. aureus (MRSA), and 21% (n=10) were Multi-Drug Resistant (MDR). Additionally, Vancomycin-Resistant S. aureus (VRSA) and Vancomycin-Resistant Enterococci (VRE) were each detected at a rate of 0.6% Conclusion Antibiotic-resistant bacteria were identified in 44 (27.7%) of samples collected from healthcare workers, indicating the presence of multidrug resistance.
The detection of methicillin-resistant Staphylococcus aureus (MRSA), along with vancomycin resistant Staphylococcus aureus (VRSA), among healthcare workers indicates a potential risk for the dissemination of highly resistant pathogens.

Background: Sand play benefits young children’s physical, cognitive, and socio-emotional development and can help meet the WHO’s daily activity recommendation of 180 minutes. Children consider playing with sand as a pleasurable activity, which allows them to interact with materials, explore, and use them creatively to develop their imagination. However, sand provides a favorable environment for the growth and development of microorganisms. Objective: To assess bacterial and parasitic contamination in playground sand and compare the findings with established hygiene standards. Materials and Methods: The study was conducted using a descriptive cross-sectional design. Thirteen kindergartens were selected through simple random sampling from a total of 39 public kindergartens in Bayangol District, Ulaanbaatar. The research was carried out using laboratory analysis methods. Collected data were compiled using Microsoft Excel and Word 2016, and processed with SPSS version 24 Results: The total bacterial contamination of the sand samples collected in the study was 2.2 Ч 10⁵ CFU/g, indicating heavy contamination. The analysis showed that Clostridium perfringens was present in 100% of samples (n=13), Escherichia coli in 92.8% (n=12), Bacillus subtilis in 46.1% (n=6), and Acinetobacter in 7.7% (n=1). Additionally, mold was detected in 38.4% of the samples (n=5). Parasite eggs were found in 54% (n=7) of the samples from the 13 kindergartens. Analysis of the types of parasite eggs revealed that Ascaris lumbricoides was present in 28.5% (n=2), Enterobius vermicularis in 28.5% (n=2), Balantidium coli in 57.1% (n=4), and Toxocara spp. in 14.2% (n=1) of the positive samples. Conclusion Playground sand is highly contaminated with bacteria and parasites, posing infection risks, and is influenced by animal feces, poor sanitation, and environmental conditions.

Introduction: Antimicrobial Resistance (AMR) has emerged as a significant public health and economic issue worldwide, affecting both developed and developing countries. Our research has been motivated by the increasing prevalence of carbapenem-resistant P. aeruginosa, A.baumannii, and K. pneumonia in recent years. Goal: To determine antibiotic susceptibility of the pathogens isolated from wound infection and to detect its multidrug resistance. Material and Method: Samples were collected from patients with wound infections. Pathogens were identified by using the traditional culture methods and the API system with serotypes determined phenotypically. Antibiotic susceptibility was assessed using the disk diffusion method and the MIC to analyse antibiotic resistance. Results: In the study, among 11,221 recorded cases of wound infections in 2023, bacterial pathogens were detected and identified at the species level in 10,339 cases (92.1%).
For identification results showed that A. baummannii were detected from 138 cases (1.3%), 216 cases (2.08%) of Ps. aeruginosa, 5 cases (0.04%) of B. cepacia, 1,383 cases (13.4%) of Enterobacteriaceae, 205 cases (2.0%) of Enterococcus spp., 8,329 cases (80.5%) of Staphylococcus spp., and 63 cases (0.6%) of Streptococcus spp.
Antibiotic susceptibility of these bacteria was assessed and determined multidrug resistance. We found the followings: For A. baumannii were resistant with MDR in 68 cases (49.3%), MRSA in 4,243 cases (51.1%), MDRSta. aureus in 1,805 cases (29.4%), VRE in 34 cases (16.5%), ESBL in 4.6%, KPC in 26 cases (11.1%), FRNF in 24 cases (11.6%), and CRPA in 13 cases (6.3%). Conclusion
1. Of all wound infections reported in 2023 in 92.1% there were detected pathogenic bacteria, of which S.aureus were in 80.5% and E. coli was in 13.4%
2. 60.7% of the pathogenic bacteria detected in wound infections were multidrug-resistant, with MRSA accounting for 51.1% and MDR A.baumannii accounting for 49.3%, indicating high antimicrobial resistance(AMR).

Background: Retinal pathologic features are associated with inflammatory processes and endothelial dysfunction, leading to circulatory abnormalities and reduced vascular reactivity. Both retinopathy and nephropathy involve thickening of basement membrane and muscular layers and increased leakage. These pathologic and hemodynamic abnormalities may occur throughout the body and their effects on the retinal vasculature may be useful indicators of cumulative microvascular damage from hypertension, inflammation, diabetes mellitus, and other processes. Type 2 MPGN is an uncommon renal condition associated with electron dense deposits in the lamina densa of the glomerular basement membrane with C3 found in the capillary loops and mesangium. The deposits in the basement membrane can lead to a breakdown of the blood-retinal barrier by interfering with the RPE layer, and type 2 MPGN has been described in association with central serous retinopathy. Purpose: To assess the retinal findings in end stage of Chronic kidney disease (CKD) and diabetic renal failure Methods: Data of diabetic renal failure (n=20, mean age 56.8±11.6), and end stage of CKD(n=83, mean age 48.2± 11.6) were cross-sectional analyzed. Nonmydriatic fundus photographs of the disc and macula in both eyes were obtained in all the subjects. The photographs were assessed by ophthalmologist using the standard protocols. The following parameters were recorded: BCVA, IOP, BP GFR, serum Creatinine, ophthalmic and fundus examination. Results: Greater severity of retinopathy was associated with DM. The presence of vascular abnormalities usually associated with Diabetes Mellitus ( DM) and was not associated with lower estimated glomerular filtration rate. All of patients with DM (n=20, 100%) ,they have Diabetic retinopathy. We found sight direct relationship between retinal posterior pole deposit with CKD patients. Some of patients who have end stage of CKD, (n=18, 11%) they have retinal deposit on their posterior pole. Conclusion Our findings show ,In participants with end stage of CKD, there was no significant severe retinopathy. In summary, our study demonstrates that DM, is one of the leading cause of irreversible vision loss. Every patients with DM need the ophthalmic follow up examination in every six months.

Description: Asymptomatic bacteriuria is defined as the presence of 1x105 or more bacteria per milliliter of urine, although there are no signs of UTI [1]. Asymptomatic bacteriuria is quite common and occurs in 1-5% of healthy women before menopause. The incidence of asymptomatic bacteriuria increases in the elderly (men and women) to 4-19%. Asymptomatic bacteriuria is observed in 0.7% -27% of patients with diabetes mellitus, in 2-10% of pregnant women and in 23-89% of patients with spinal cord injury. Asymptomatic bacteriuria is rarely observed in young men and always requires differential diagnosis with chronic bacterial prostatitis[2].
Among the elderly, those in institutionalized settings such as long-term care facilities and hospitals have a higher prevalence of asymptomatic bacteriuria than those living in the community. Therefore, interest in asymptomatic bacteriuria has shifted from the general population to these groups of individuals. The prevalence varies from 2% to 10% in pregnancy and from 15% to 50% in the elderly in long-term care facilities.[3,4]
This study was undertaken to determine the prevalence of people asymptomatic bacteriuria in among hospitalized people. Purpose: Investigate cases of asymptomatic bacteriuria among hospitalized individuals and determine antibiotic susceptibility by type of pathogen detected in urine. Materials and methods: This cross-sectional study in the “I” Hospital of UB city, Mongolia. We conducted into the study 133 patients. After obtaining informed consent, a midstream clean-catch urine sample was collected in the same 24 hour time window and was evaluated for urinalysis using a dipstick test and routine culture. A positive urine culture was defined as a single urine sample with microbal growth of >10⁵ colony- forming units of a single organism. We use 5-10% Blood agar, UTI agar, Mackkong agar and Chroma agar for a bacterial growth. Then we did antibiotic susceptibility of the determined pathogen. Result: The average age of the respondents was 55.9±14.6, the youngest was 17 years old and the oldest was 91 years old (CI 95% 53.4-58.5). By age group, 4.5% (n = 6) of people aged 20-30, 21.1% (n = 28) of people aged 31-45, 37.5% (n = 50) of people aged 46-60, and people over 61 years of age 36.8% (n = 49) respectively.Of the respondents, more than 56 or 54.9% (n = 73) were elderly, of which 63.9% (n = 85) were women and 36.1% (n = 48) were men.
A total of 36 (27.1%) leukocytes were detected, and 25 (34.2%) more (P-0.05) were detected in people over 56 years of age. The protein detected in 24 (18.0%) people, all over 56 years of age and more defined in men (P-0.01).
80.5% (n = 107) of those surveyed were diagnosed with the pathogens, and 54.9% were elderly (P-0.031). 82.4% of women and 77.1% of men were diagnosed with urinary pathogens.
Streptococcus 26%, E.coli 19%, Saprophyticus 13%, Enterococcus 11% and Staphylococcus 11% were detected in the analysis, and Streptococcus and E.coli (19%) accounted for a slightly higher rate of infection.
A total of 13 types of pathogens were identified, of which 53.4% were susceptible, 21.7% were moderately sensitive, and 24.9% were non-susceptible. Discussion: According to a study conducted by researchers at the University of Taiwan, the prevalence of asymptomatic UTI-s in the general population is 57.8%. E.Coli was also the most common bacterium in the urine [6].
In our study, there are many cases of E. coli.
Study in the United States found that the prevalence of asymptomatic bacteriuria among inpatients was 8.5%, all of them women. The most common pathogens in this study are Enterococcus bacteria. This study found that 1 in 10 people admitted to the hospital may have asymptomatic bacteriuria. [1] Conclusion
1. Asymptomatic bacteriuria in 80.5% of those surveyed 54.9% were detected in people over 56 years of age.
2. Antibiotic susceptibility of the tested pathogens was determined as 53.4% sensitive, 21.7% moderately sensitive and 24.9% insensitive.

Introduction: Major outbreaks of infectious diseases caused by enteroviruses (EV) have been reported in recent years. As of 2017, in Mongolia, hand, foot and mouth disease, or HFMD, accounts for 13.7% of all infectious diseases. The purpose of this study was to investigate the circulation of EV among healthy children, and clinical characteristics of infection. Materials and Methods : The analysis of EV circulation was conducted on Polio Laboratory data of 1172 specimens, collected in 2013-2018, from 9 districts of UB and 21 provinces of Mongolia. Also specimens of 239 patients, who underwent outpatient and inpatient treatment at the NCCD in 2014-2018, were randomly selected and stool samples were collected. Clinical symptoms of patients were analyzed and virus isolation analysis was performed in order to confirm the diagnosis. Results: Children from 1 month of age to 5 years have participated in the study and the average age was 2 years and 5 months. The enterovirus rate among healthy children was 20.1%. Virus circulation rate was highest in 2013 (37.2%), lowest in 2014 (11.8%). The peak of circulation is observed in May and October, while the minimum rate is in July.
Out of selected patients 52.7% (n=126) were diagnosed with HFMD, rash infection occurred in 28.5% (n=68), acute flaccid paralysis-18.8% (n=45). EV was detected in 56.4% (n=135) of the collected stool samples.
Children from 1 month of age to 5 years have participated in the study and the average age was 2 years and 5 months (95% CI:2.5 ±0.1). 49% (n=578) of participants were female, 50.7% (n=594) were male. The enterovirus rate among healthy children were 20.1% (n=236; 95% CI: 20.1 ± 0.55). Virus circulation rate was highest in 2013 (37.2%), lowest in 2014 (11.8%). The peak of circulation is observed in May and October, while the minimum rate is in July. Although the enterovirus isolation rate was relatively high among children under 3, there was no statistically significant difference (p>0.05), as well as there was no difference in gender (p>0.05). As for the EV circulation by region, the highest prevalence rate is found in Central region (27.5%), while the lowest is in the Eastern region (12.7%) with no statistical significance by regions (p>0.05). Conclusions Among children, HFMD is a common disease, that caused by EV. Enterovirus infection can often cause fever, flu like symptoms as well as spotted, maculopapular rash. The EV isolation rate of 20.1% indicates that the incidence of enterovirus is characterized by symptoms of influenza-like illness or is asymptomatic.

Background: We aimed to study the etiology and transmission route of diseases introducing the modern, rapid and high-sensitivity molecular genetic diagnostic methods for salmonellosis. Material and Method: In the study, we collected 680 stool samples and defined organisms of food intoxication by identification of bacteria, polymerase chain reaction (PCR) and determined serotype and antibiotic resistance. Result: Salmonella spp was detected from the stool of 25 (42.3%) patients out of 59 outpatient clinic and of 170 (27.4%) patients out of 621 inpatient clinic with diagnosis of food intoxication. In total there was detected 195 salmonella spp, and out of this isolated Sal. typhimurum in 193 (98.9%), and Sal. enteritidis was in 2 (1.1%) patients, respectively. We defined Sal. typhimurum in selected 32 cultures and did not detect resistant gene DT-104 ACS-SuT by PCR. Conclusion As resulted in the survey, we defined 195 (28,6) Salmonella typhimurum among the 680 patients who were suffered from food intoxication, and revealed fast foods, animal derived foods such as chicken, fish caused the food intoxication. Sal. typhimurium not resistance to antibiotics.

INNOVATION DENT • VOL. 3 • No.1 • Mar 2017 35 Background Odontogenic infection is defined as most dangerous inflammatory diseases in dental practice, it is believed that the cause of tooth decay disease and its complications currently a major cause of oral infections, or bacterial infection of the main sources of these diseases. Methods Between 2010-2011, odontogenic cellulitis in 21 patients were studied for aerobic and anaerobic bacteria by the Clinic bacterial laboratory in NCCD. Results 21 patients participated, were 10 men and 11 women with odontogenic cellulitis. Aerobic bacteria only were recovered in 3 (14%), anaerobic bacteria were recovered 8 (38%), mixed aerobic and anaerobic bacteria were recovered in 10 (48%). Conclusion A certain percentage of aerobic and anaerobic bacteria in odontogenic cellulitis shows simple space infection caused by aerobic bacteria, other complication of odontogenic cellulitis and multiple spaces infection caused by anaerobic and mixed bacterial infection. During dontogenic cellulitis determined high percentage of anaerobic and mixed bacterial infection, thus is our study have shown detection of anaerobic bacterial infection is important clinical significance in dental practice.

There are a lot of influencing factors of facial nerve palsy; experts believe that is most likely caused by a Virus (54%) and Bacterial infections. Noninfectious causes of facial nerve palsy induce tumors (28%) and less commonly influences head trauma (18%). The retrospective analysis of WHO, in 2012. There are some cases of postoperative complication in middle ear surgery is facial nerve palsy and the total recovery outcome of function was not good. From 2013 to 2016 in EMJJ hospital, Mongolia, we enrolled 16 cases with facial nerve damaged in intratympanic canal but we could not recruit some patients with facial palsy over 6 months. Each subject was tested with pure tone test, ABR, Tympanometry. These were performed for the detection of hearing loss after Temporal bone injury. Then we also investigated location of facial nerve damages of patients by MRI and CT before reconstructive surgery. After that surgery, all patients were given corticosteroid treatment (20mg/day) and physical therapy performed such as acupuncture for a week. Study results revealed that 6 cases after 18 days, 2 cases after 30 days, 1 patient after 45 days of reconstructive surgery regained good symmetry. Therefore, we considered that, postoperative treatments like physical therapy with B12, steroid had good benefits for operation result and to shorten the recovery time. There was a patient who had damaged facial nerve in the tympanic segment during Mastoidectomy. In that case, we performed cable nerve grafting using the r.auricularismagnium but we could not recover facial nerve function. Traumatic facial nerve paralysis is the second most common type. We discussed that performing reconstruction surgery within first 3 months after intratemporal facial nerve injury is extremely desirable and more effective. In our opinion, nerve recovery might be not successfully cause of injured myelin sheet of facial nerve during middle ear surgery.

Extraintestinal pathogenic Escherichia coli (ExPEC), the specialized strains ofE.coli that cause most extraintestinal infections, represent a major but littleappreciated health threat. Phylogenetic analysis has shown that ExPEC is composedof four main phylogenetic groups (A,B1, B2, and D) and that virulent extraintestinalstrains mainly belong to groups B2 and D.In this study, we aimed to assess therelation between adherence virulence and phylogenetic groups of ExPEC.A total of 161 E.coli samples were collected. Out of these 17 (10.6%) werefrom pus, 66 (41 %) from urine, 78 (48.4%) from cervical swab. The phylogeneticgroups and 6 virulence genes (fimH, papC, papGII, papGIII, fa/draBC,andSfa/focDE) encoding adhesins were identified by triplex PCR. Phylogeneticgroups distribution was as follows: B1 10.5%, A 24.7%, B2 25.3%, and D 38.9%. Virulence genes prevalence was fimH 90.1%, papC 23%, papGII 16.8%, papGIII1.9%, Afa/draBC 11.8%, andSfa/focDE 5.6%. The cell surface protein (curli) wasdetected 50,3% by Congo red agar. In conclusion: The most isolated strainsbelonged to the phylogenetic group B2 and D. The phylogenetic groups weresignificantly associated with some genes encoding adhesins (fimH, papC) and cellsurface protein (curli).