Background: Currently, colorectal cancer (CRC) ranks as the third most common cancer and the second leading cause of cancer-related mortality worldwide. CRC frequently metastasizes to the liver (50%), lungs (10–15%), peritoneum (4%), bones (10.7%–23.7%), brain (0.3%–6%), and spinal cord. Approximately 35% of CRC cases are diagnosed before distant metastasis, 36% upon lymph node involvement, and 23% after distant organ metastasis. Although several studies have established primary tumor models in mice in our country, there are limited studies on experimental lung metastasis models, prompting the need for this research. Aim: To establish and evaluate a lung metastasis model of colorectal cancer in C57BL/6J mice using the MC38 cell line. Materials and Methods: This study was conducted at the Institute of Biomedical Sciences, Mongolian National University of Medical Sciences. Approval was obtained from the Ethics Review Board of the Mongolian National University of Medical Sciences (2023/3-09) and all laboratory safety regulations and protocols were strictly followed. Male C57BL/6J mice bred at the Experimental Animal Center of Mongolian National University of Medical Sciences were used. MC38 murine colorectal carcinoma cells were cultured and injected intravenously (via the tail vein) at a concentration of 0.25×10⁶ cells per mouse (n=12) to induce lung metastasis. Histological analysis was subsequently performed. Results: Histological examination revealed significant alterations in lung tissue architecture, characterized by areas of dense infiltration by pleomorphic, hyperchromatic cells, disrupting the normal alveolar structure. No histological abnormalities were observed in other organs. Conclusion Intravenous injection of MC38 colorectal adenocarcinoma cells into the tail vein of C57BL/6J mice successfully induced lung metastases, characterized by hyperchromatic, pleomorphic cell infiltrates forming glandular structures within the lung parenchyma.

Background: There are 240 million children with disabilities worldwide, of whom 34 million have hearing and speech impairments; in Mongolia, 1,538 children are affected.¹, ³ Due to barriers in health care, education, employment, social services, and living environments, people with disabilities face limited opportunities to fully participate in social life. In addition to encountering greater obstacles, households with members with disabilities experience poverty levels twice as high as those without.⁴ While studies on children with disabilities in Mongolia have addressed issues related to education, health, and access to social services, little research has been conducted on family quality of life and the factors influencing it. Aim: This study aims to assess the quality of life of families with children who have hearing and speech impairments and to identify factors that influence it. Materials and Methods: The quality of life of families with children who have hearing and speech impairments was assessed using a standardized questionnaire developed by researchers at the University of Kansas, United States, which examines five core domains of family quality of life. A total of 120 parents and caregivers of children aged 0–18 years with hearing and speech impairments were included in the study Results: The mean family quality of life score among families of children with hearing and speech impairments was 3.79±0.61. Among the subdomains, the highest scores were reported for parental role (3.94) and family interactions (3.92), while emotional well-being received the lowest score (3.57). Factors influencing family quality of life included the presence of comorbid disabilities in the child, the caregiver’s self-rated health, stress level, family size, and adequacy of household income. Conclusion The quality of life of families raising children with hearing and speech impairments was found to be generally good. Factors influencing family quality of life included the type of child’s disability, caregiver’s age, health status, stress level, family size, and adequacy of household income.

BACKGROUND. The Mongolian traditional medicine Anar-5 is excellent for weak digestion and helps with stomach irritation, loss of appetite, and resulting body weakness. Anar-5 blends punicagranatum, cinnamomum cassia presl, piper longum, cardamom and alpiniaofficinarum. We are establish an experimental animal of chronic gastritis to investigate the effect of traditional medicine Anar-5 on rats gastric mucosa. Methods: In this study, the protective effect preparation in sixty five healthy, male wistar rats were treated with intragastric administration of ammonia water 0.1%. To rats in three experiments for 2 week, 4 week, and 6wk, gastric tissues were examined histopathologically for atrophic changes and blood’s gastrin produced by preparation treatment. Results: After the treatment of animals blood’s gastrin was significantly different from that in control group (p<0.05), and the gastric mucosal inflammation was infiltration of inflammatory cells, decreased thickness of lamina propria. Conclusion: Treatment with preparation from Anar-5 protectived by the chronic gastritis and gastric atrophy.

BackgroundThese study made us to investigate the drug row material of Mongolian traditional medicine such us Tribulus terrestris, Malva neclecta Wall and Eriocheir sinensis compounded preparation named “Malbus”. Investigated us preparations steel using for treatment of kidney and urinetract deseases, it is one of effective in Mongolian traditional drug preparations.Material and MethodsThe experimental protocol was approved by the Ethics Review Committee at the Ministry Health of Mongolia. In research we used 20 healthy mice and 60 Wistar rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Producttion Corporation and pathological laboratory of Institute Veterinary medicine. Chemically acting substances is steroid saponin content in Malbus compound was detected by using thin layerchromatography (TLC) and its value was determined by UV-spectrophotometry. The acute oral toxicity study was according to the method Prozorovsky (1978). The toxic nephrosis was produced using Gentamicin (80 mg/kg) rats by using Neugarten’s method (1983).ResultsThe ethanolic extract results of the phytochemical investigations showed that conteined in the “Malbus” determined the presence of bioactive substances such as flavonoids, steroid saponins. The ethanolic extract of Malbus was found to be LD50 of 16.3 g/kg. Toxic nephrosis was induced in Wistar rats administered preparation Malbus dose 80 mg/kg, 160 mg/kg, and 240 mg/kg. Blood levels of creatinine, uric acid, and urea were siginificantly reduced by Malbus treatment compared tocontrol. Histological study revealed that Malbus was effective for treatment of nephritis in rats induced by gentamicin.Conclusions:1. The ethanolic extract of “Malbus” compound determined the presence of bioactive substances such as flavonoids, steroid saponins.2. LD50 of Malbus 16.3 g/kg, the preparation is has nephroprotective effect on experimental Gentamicine induced nephrosis in rats.

This study was carried out to investigate the pharmacological effects of aqueous extracts from the traditional Mongolian medicinal herb, the root of Caraganajubata pall poir, on liverfibrosisinduced carbon tetrachloride (CCl4) in rats. Liverfibrosis was assessed by histological observations and serum enzyme activities. Treatment with aqueous extract of Caraganajubata significantly reduced the levels of liver ASAT and ALAT. Aqueous extract of Caraganajubata root inhibits fibrosis and reduced serum enzyme in rat liverinduced by CCl4.

In last years we have produced a combination species from medicinal plants which are used in traditional medicine and has tendentious the increasing of needs and production of plant preparations from natural resources. Although traditional medicine has being increased to be used last years, standardization of them is not sufficient. The standardization is important to ensure the safety, effectiveness and quality of traditional medicine. This research was made with purposes for determining of qualitative and quantitative analyses of biological active substances in the preparation such as Jonsh-5 is utilized to prevent osteoporosis, to accelerate bone ankylose in period of injure. The compositions of the preparation Jonsh-5 are Calcitum, Carduus crispus L. Inula helenium L.Terminalia chebula Retz, Forsutima suspense Thunb. Vahl.In order to succeed the above mentioned aim, we have achieved the following results: 1. The chromatographical method and methodology for the qualitative and quantitative analyses of main compounds in the preparation Jonsh-5 were developed using HPLC. 2. Quantitative analyses of main compounds of the compositions in the preparations were defined by HPLC method. 1.000gr of the preparation Jonsh-5 involves gallic acid 1.11 mg, alantolaktone 1.77 mg.