Triple-negative breast cancer (TNBC) represents an aggressive breast cancer subtype with poor prognosis and limited targeted treatment options. This investigation examined the anti-cancer potential of Caerulomycin A (Cae A), a natural compound derived from marine actinomycetes, against TNBC. Cae A demonstrated selective inhibition of viability and proliferation in TNBC cell lines, including 4T1, MDA-MB-231, and MDA-MB-468, through apoptosis induction. Mechanistic analyses revealed that the compound induced sustained endoplasmic reticulum (ER) stress and subsequent upregulation of C/EBP homologous protein (CHOP) expression, resulting in mitochondrial damage-mediated apoptosis. Inhibition of ER stress or CHOP expression knockdown reversed mitochondrial damage and apoptosis, highlighting the essential role of ER stress and CHOP in Cae A's anti-tumor mechanism. Both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) decreased in TNBC cells following Cae A treatment, indicating reduced mitochondrial respiratory and glycolytic capacities. This diminished energy metabolism potentially triggers ER stress and subsequent apoptosis. Furthermore, Cae A exhibited significant anti-tumor effects in the 4T1 tumor model in vivo without apparent toxicity. The compound also effectively inhibited human TNBC organoid growth. These results indicate that Cae A may serve as a potential therapeutic agent for TNBC, with its efficacy likely mediated through the disruption of glucose metabolism and the induction of ER stress-associated apoptosis.
Humans ; Endoplasmic Reticulum Stress/drug effects* ; Triple Negative Breast Neoplasms/genetics* ; Apoptosis/drug effects* ; Cell Line, Tumor ; Female ; Animals ; Glucose/metabolism* ; Mice ; Cell Proliferation/drug effects* ; Transcription Factor CHOP/genetics* ; Antineoplastic Agents/pharmacology* ; Mitochondria/metabolism* ; Mice, Inbred BALB C

Objective:This study aims to analyze the current status and risk factors of suicidal ideation in inpatients with schizophrenia and develop prediction models to improve the identification of individuals at high risk of suicide attempts.Methods:A total of 252 inpatients with schizophrenia were grouped with a 7∶3 ratio into modeling group ( n=176) and validation group ( n=76). The Beck Scale for Suicide Ideation (BSI), the Hamilton Anxiety Scale (HAMA), the Hamilton Depression Scale (HAMD), the Social Support-Rating Scale (SSRS), the Brief Psychiatric Rating Scale (BPRS), the Pittsburgh Sleep Quality Index (PSQI) were used to assess suicidal ideation, anxiety symptoms, depressive symptoms, social support, psychotic symptoms, and sleep quality, respectively. According to the presence of suicidal ideation, all patients were classified into those with and without suicidal ideation. The difference in each potential risk factor between the two groups was tested using the independent sample t-test and χ 2 test as appropriate. Multivariate Logistic stepwise regression analysis was used to identify independent risk factors of suicidal ideation. The R 4.1.2 software was used to construct the risk prediction model and draw a nomogram to visualize the Logistic regression model. The receiver operating characteristic (ROC) and the area under curve (AUC) were calculated to evaluate the predictability of this model. Internal validation of the model was examined using the Boostrap validation method. Results:Of the 252 inpatients with schizophrenia, 92 patients experienced suicidal ideation, with an incidence of 36.51%. The Logistic regression model revealed that gender (β=0.70, OR=3.66, 95% CI=1.02-3.85), education (β=-0.54, OR=0.85, 95% CI=0.19-0.89), chaperonage (β=1.32, OR=6.72, 95% CI=1.38-10.16), anxiety symptoms (β=0.08, OR=3.98, 95% CI=1.01-1.18), depressive symptoms (β=0.11, OR=28.30, 95% CI=1.07-1.16) were independent risk factors of suicidal ideation in inpatients with schizophrenia (all P?0.05). The AUC of the modeling group and validation group were 0.83 and 0.82, respectively. Conclusion:The incidence of suicidal ideation was high in inpatients with schizophrenia. The Logistic risk prediction model constructed in this study has good predictive efficacy, which can provide a theoretical basis for early identification of individuals at high risk of suicide attempts.

Objective:This study aims to analyze the current status and risk factors of suicidal ideation in inpatients with schizophrenia and develop prediction models to improve the identification of individuals at high risk of suicide attempts.Methods:A total of 252 inpatients with schizophrenia were grouped with a 7∶3 ratio into modeling group ( n=176) and validation group ( n=76). The Beck Scale for Suicide Ideation (BSI), the Hamilton Anxiety Scale (HAMA), the Hamilton Depression Scale (HAMD), the Social Support-Rating Scale (SSRS), the Brief Psychiatric Rating Scale (BPRS), the Pittsburgh Sleep Quality Index (PSQI) were used to assess suicidal ideation, anxiety symptoms, depressive symptoms, social support, psychotic symptoms, and sleep quality, respectively. According to the presence of suicidal ideation, all patients were classified into those with and without suicidal ideation. The difference in each potential risk factor between the two groups was tested using the independent sample t-test and χ 2 test as appropriate. Multivariate Logistic stepwise regression analysis was used to identify independent risk factors of suicidal ideation. The R 4.1.2 software was used to construct the risk prediction model and draw a nomogram to visualize the Logistic regression model. The receiver operating characteristic (ROC) and the area under curve (AUC) were calculated to evaluate the predictability of this model. Internal validation of the model was examined using the Boostrap validation method. Results:Of the 252 inpatients with schizophrenia, 92 patients experienced suicidal ideation, with an incidence of 36.51%. The Logistic regression model revealed that gender (β=0.70, OR=3.66, 95% CI=1.02-3.85), education (β=-0.54, OR=0.85, 95% CI=0.19-0.89), chaperonage (β=1.32, OR=6.72, 95% CI=1.38-10.16), anxiety symptoms (β=0.08, OR=3.98, 95% CI=1.01-1.18), depressive symptoms (β=0.11, OR=28.30, 95% CI=1.07-1.16) were independent risk factors of suicidal ideation in inpatients with schizophrenia (all P?0.05). The AUC of the modeling group and validation group were 0.83 and 0.82, respectively. Conclusion:The incidence of suicidal ideation was high in inpatients with schizophrenia. The Logistic risk prediction model constructed in this study has good predictive efficacy, which can provide a theoretical basis for early identification of individuals at high risk of suicide attempts.

Objective:To explore the correlation between screen sedentary behavior and sleep quality of the elderly people in the community, so as to provide a reference for improving sleep quality of the elderly people in the community.Methods:From November 2021 to April 2022, 627 older adults from 3 communities in Hefei, Anhui Province were selected as the research objects using the convenience sampling method. The General Information Questionnaire, Measure of Older Adults' Sedentary Time Questionnaire, and Pittsburgh Sleep Quality Index (PSQI) were used to investigate the elderly people in the community. Pearson correlation analysis was used to analyse the correlation between screen sedentary behavior and sleep quality of the elderly people in the community. The influencing factors of sleep quality were assessed by Binary Logistic regression. A total of 660 questionnaires were distributed and 627 valid questionnaires were recovered, with an effective recovery rate of 95.0% (627/660) .Results:The screen sedentary behavior time of 627 elderly people in the community was (3.53±1.52) h/d, the total score of PSQI was (7.11±3.58) , and the incidence of sleep disorders was 37.48% (235/627) . Pearson correlation analysis showed that screen sedentary positively correlated with the total score of PQSI ( r=0.466, P<0.01) . Binary Logistic regression analysis results showed that the screen sedentary behavior ( OR=1.892, 95% CI: 1.638-2.185) , female ( OR=1.963, 95% CI: 1.120-3.441) , self-reported poor health status ( OR=3.332, 95% CI: 1.380-8.043) were the risk factors of sleep quality of the elderly people in the community. Conclusions:The screen sedentary behavior and sleep quality of the elderly people in the community are not optimistic, and the screen sedentary behavior, female, self-reported poor health status are the risk factors of sleep quality of the elderly people in the community. Community health staff should develop targeted intervention strategies to help the elderly people in the community reduce screen sedentary behavior time and improve sleep quality.

Objective:To establish a nursing intervention process for Intensive Care Unit (ICU) patients with thirst after cardiac surgery to guide clinical practice, so as to provide a basis for the scientific management of thirst in patients after cardiac surgery.Methods:We searched domestic and foreign databases to obtain and evaluate relevant literature on thirst in critically ill patients. After discussion by the research group, the first draft of the thirst intervention process was formulated. The Delphi method was used to conduct two rounds of expert consultation, and finally the nursing intervention process for ICU patients with thirst after cardiac surgery was determined.Results:Through two rounds of expert consultation, 4 first-level indicators (pre-intervention evaluation, graded intervention, post-intervention evaluation, health education) , 11 second-level indicators, and 29 third-level indicators were formed. The authority coefficients of the two rounds of expert consultation were 0.83 and 0.85 respectively, and Kendall's W were 0.259 and 0.214 respectively. Conclusions:Expert advice tends to be consistent, highly authoritative, and good coordination, which can provide a scientific management basis for the construction of nursing intervention process for ICU patients with thirst after cardiac surgery.

Thirst is one of the common complaints of critically ill patients, persistent and intense thirst is closely related to a variety of negative emotions, such as anxiety, depression, etc., which can cause restlessness, sleep disorders, and reduce the quality of life; at the same time, it can cause bacterial growth in the patient′s oral cavity, increasing the risk of secondary infection, thereby delaying the recovery of the disease and prolonging the length of ICU hospitalization. Therefore, this article reviews the current status, risk factors, and intervention strategies of thirst in critically ill patients in order to provide a reference and basis for medical staff to carry out thirst intervention measures in critically ill patients.

Objective To analyze the risk factors for lung infection in patients with craniocerebral injury after tracheotomy through Meta-analysis and provide a scientific reference for its prevention. Methods The inclusion and exclusion criteria were determined and the search strategies were designed. Two researchers independently searched both English and Chinese databases such as Cochrane Library, EMBASE, PubMed, CNKI, Wanfang and VIP (taking brain injury, craniocerebral injury, traumatic brain injury, tracheotomy, lung infection, pulmonary infection, risk factor, influencing factor and promotive factor as the key words) for literatures published since the databases were built to April 28th, 2019. RevMan 5.3 was used to analyze the data after the quality of literatures were assessed. Results Totally 9 articles were included, including 2112 patients with craniocerebral injury. Meta-analysis showed that diabetes [OR=3.36, 95％CI(2.49, 4.54),P< 0.01], use of hormones [OR=2.52,95％CI(1.83, 3.45),P< 0.01], Glasgow Coma Scale (GCS) score [OR=2.92,95％CI(1.95, 4.37),P< 0.01], hypoproteinemia [OR=3.18,95％CI(2.20, 4.60), P< 0.01], aspiration [OR=3.18, 95％CI(2.24, 4.51),P < 0.01], smoking [OR=1.87,95％CI(1.16, 3.00), P < 0.01], non-irrigable tracheal cannula [OR=2.54, 95％CI(1.71, 3.77),P<0.01]and tracheotomy time (>7 d) [OR=2.32, 95％CI (1.42, 3.79);P< 0.01]were the influencing factors for lung infection in patients with craniocerebral injury after tracheotomy. Conclusions Existing evidence shows that combined with diabetes, use of hormones, high GCS score, hypoalbuminemia, occurrence of aspiration, use of non-irrigable tracheal cannula, smoking, tracheotomy time > 7 d were independent risk factors for lung infection in patients with craniocerebral injury.

Objective:To systematically evaluate the qualitative research on experience of nurses in participating in treatment of acute respiratory infectious diseases, so as to provide evidence-based basis for subsequent formulation of intervention strategies and practices.Methods:Databases including PubMed, EMbase, Web of Science, The Cochrane Library, CNKI, Wanfang, and VIP databases for related qualitative research.The retrieval time was from the construction of databases to February 13, 2020. The 2017 version of JBI Critical Appraisal Tool for qualitative studies of Australian Evidence-Based Health Care Center was used for literature quality evaluation, and the collection and integration methods were adopted for Meta integration.Results:A total of 8 studies were included, 30 research results were refined, 8 new categories were formed and 3 integrated results were obtained. At the beginning of the epidemic, nursing staff volunteered themselves and showed a series of positive psychological experiences. As the treatment progressed, the nursing staff were under tremendous physical and psychological pressure and heavy workload. Through self-adjustment and encouragement and support from all parties of the society, the nursing staff finally realized their professional value, gained growth and put forward expectations and suggestions to the hospital management staff.Conclusions:The nurses are under great psychological pressure during the treatment and it should be taken seriously. In the process of participating in the treatment, it is necessary to dynamically evaluate psychological condition of nursing staff and timely provide support, guidance and encouragement to improve their psychological coping ability.

Objective Cancer-related fatigue (CRF) is a key in the management of cancer patients' clinic syptoms.This article investigated the status quo of nurses' knowledge and attitude towards CFR.Methods THe method of cross-sectional survey and questionnaire was used to investigate the knowledge and attitude towards CRF among nurses from related departments of three Grade III hospitals in Nanjing.Results 142 nurses answered the questionnaire.The average correct rate was 76.25%, among which nurses from the oncology department had better congition rate than nurses from other medical and surgical departments (84.3%, 75.98%, 79.57%) , representing significant difference (P<0.05).64.79% of the nurses found the relatives of cancer patients and nurses often fail to understand cancer patients;complaint of fatigue, 76.76% of nurses assumed there is lack of communication in fatigue between patients and medical staff.94.36% of nurses agreed medical institutions should strengthen the management of CRF.Conclusion At present, the clinical nurses have inadequate knowledge about CRF, which should be enhanced in future work.

Objective To investigate the curative effect and nursing of medical ozone autohemotherapy in clinical treatment. Methods 45 cases of cerebral infarction patients with medical ozone self blood therapy spells, psychological guidance and health education before operation in the sterile operation, postoperative nursing, education results. Results 45 cases of cerebral infarction in patients with very good completed treatment, 2 cases because of dizziness aggravated, 2 cases of vascular puncture for difficult to stop treatment, the rest were different extent achieved certain effect. Conclusions the clinical experiment showed that the nursing, preoperative education, psychological guidance and sterile operation, postoperative is an effective measure for ensuring medical ozone autohemotherapy smooth finish.