Objective To establish a new program of blood cell processing apparatus(NGL-BBS)for the preparation of washed mixed plateletsand to study the effect on biological activity of platelets compared with tradi-tional manual method.Methods Mixed concentrated platelets were separated and prepared from whole blood by white membrane method.Blood cell processing apparatus with new program set(experimental group)and manual method(control group)was used for thepreparation of washed mixed platelets.The expression rate of CD62P and CD63 in the two groups of washed mixed platelets was compared by flow-cytometry.Thrombus elastography(TEG)was used to measure and compare the MA value between the two groups.Results The expression rate of CD62P and CD63 in the experimental group was lower than that in the control group(t = 4.11,P<0.01;t = 10.78,P<0.01).TheTEG MA valueof the experimental group was higher than that of the control group(t = 6.67,P<0.01).Conclusion The present study demonstrates that the use of NGL-BBS for the preparation of washed mixed plate-lets has a lesser impact on biological activity compared to manual preparation methods.

A 2-year-old girl was diagnosed as Weill-Marchesani syndrome with typical systemic features of short stature, short and stubby hands and feet, language disorders and mental retardation. He developed bilateral angle closure glaucoma, ectopia lentis and suffered visual loss from the ocular features of Weill-Marchesani syndrome. The child was successfully treated by combined CO2 laser-assisted sclerectomy surgery and trabeculectomy.

Lung cancer is the most commonly diagnosed cancer and the leading cause of cancer death. Although great progress has been made in chemotherapy, radiotherapy and targeted therapy, the emergence of acquired drug resistance hinders the efficacy of clinical treatment. Studies have shown that tumor is a class of diseases with damaged cell cycle regulation mechanism, in which checkpoint kinase (Chk) plays a core role, Chk1 and Chk2 are very important protein kinases in the checkpoint. In recent years, it has been found that the regulation of Chk1 and Chk2 plays an important role in the clinical treatment and drug resistance mechanism of lung cancer. This article reviews the mechanism of cell cycle checkpoint kinase and drug resistance of lung cancer, and expounds the effective therapeutic targets and methods of lung cancer.
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Dysfunction of the Hippo pathway enables cells to evade contact inhibition and provides advantages for cancerous overgrowth. However, for a significant portion of human cancer, how Hippo signaling is perturbed remains unknown. To answer this question, we performed a genome-wide screening for genes that affect the Hippo pathway in Drosophila and cross-referenced the hit genes with human cancer genome. In our screen, Prosap was identified as a novel regulator of the Hippo pathway that potently affects tissue growth. Interestingly, a mammalian homolog of Prosap, SHANK2, is the most frequently amplified gene on 11q13, a major tumor amplicon in human cancer. Gene amplification profile in this 11q13 amplicon clearly indicates selective pressure for SHANK2 amplification. More importantly, across the human cancer genome, SHANK2 is the most frequently amplified gene that is not located within the Myc amplicon. Further studies in multiple human cell lines confirmed that SHANK2 overexpression causes deregulation of Hippo signaling through competitive binding for a LATS1 activator, and as a potential oncogene, SHANK2 promotes cellular transformation and tumor formation in vivo. In cancer cell lines with deregulated Hippo pathway, depletion of SHANK2 restores Hippo signaling and ceases cellular proliferation. Taken together, these results suggest that SHANK2 is an evolutionarily conserved Hippo pathway regulator, commonly amplified in human cancer and potently promotes cancer. Our study for the first time illustrated oncogenic function of SHANK2, one of the most frequently amplified gene in human cancer. Furthermore, given that in normal adult tissues, SHANK2's expression is largely restricted to the nervous system, SHANK2 may represent an interesting target for anticancer therapy.

Lung cancer is the most common malignant tumor in the world with the highest incidence of deaths. In recent years, the treatment of lung cancer has made a significant breakthrough. However, as the tumor progresses, lung cancer cells inevitably acquire resistance and the efficacy of the treatment are greatly reduced. P21 protein plays a dual role in tumors, which not only regulates the cell cycle, induces apoptosis, inhibits cell proliferation, but also protects cells against apoptosis and promotes tumor cell resistance. This article reviews the research on P21 and lung cancer resistance, to provide new ideas for individualized treatment of lung cancer and overcoming lung cancer resistance.

Objective To study the effects of morphine on serum concentrations of cortisol (COR), adrenaline (ADR), noradrenaline (NAD) and dopamine (DOP) in premature infants requiring mechanical ventilation (MV).Method From January to November, 2017, premature infants (gestational age: 28～36 weeks and body weight :1 000～2 499 g) admitted to neonatal department of our hospital and received mechanical ventilation were prospectively studied.The participants were randomly assigned into morphine group and control group.Modified neonatal pain assessment scale ( NIPS ) and Ramsay scores were calculated at the beginning of MV (before the administration of morphine ) and at different time points after MV (2 h, 12 h, 24 h and 48 h after the administration of morphine ).Serum concentrations of COR , ADR, NAD and DOP were also measured at the beginning of MV and 24 h after MV/morphine.The scores and concentrations were compared between the two groups.Result A total of 60 infants were included, with 30 infants in each group.The modified NIPS and Ramsay scores and serum concentrations at the beginning of MV were similar between the two groups ( P＞0.05).The modified NIPS and Ramsay scores at 2 h, 12 h, 24 h and 48 h after the initiation of MV were significantly lower in the morphine group comparing with the control group[2 h: (0.8 ±0.5) vs.(2.9 ±0.7), (0.9 ±0.6) vs.(2.3 ±0.6); 12 h: (1.1 ±0.6) vs.(2.8 ±0.8), (1.3 ±0.7) vs.(2.3 ±0.8); 24 h: (1.4 ±0.8) vs.(3.0 ±0.7), (1.6 ±0.6) vs. (2.4 ±0.8); 48 h: (1.3 ±0.7) vs.(2.7 ±0.9), (1.7 ±0.7) vs.(2.5 ±0.8)].Furthermore, serum concentrations of COR, ADR, NAD and DOP at 24 h after MV were also significantly lower in the morphine group [COR: (165.4 ±25.0) nmol/L vs.( 319.8 ±71.2) nmol/L, ADR: ( 30.3 ±4.5) ng/L vs. (45.4 ±4.3) ng/L,NAD: (138.0 ±16.5) ng/L vs.(230.5 ±26.1) ng/L, DOP: (43.6 ±7.2) ng/L vs.(86.3 ±10.0) ng/L, P＜0.05].Conclusion Morphine is associated with lower serum concentration of catecholamine and lower NIPS scores during MV in premature infants.

Objective: To evaluate the usage of ultrasound guided wire-localization, nano-carbon staining and the combination of the above two methods in detecting sentinel lymph node (SLN) in breast cancer. Methods: A total of 159 cases of breast cancer from May 2015 to December 2017 in Shanxi Provincial Cancer Hospital were selected, and they were treated with ultrasound guided wire-localization, nano-carbon staining and combination of the two methods separately to detect SLN before the operation. After the operation, SLN and axillary lymph node in each group were marked and made pathological diagnosis. Results: There were 69 cases with pathological diagnosis of SLN metastasis and 90 cases without abnormal representation. With the patient as the unit, the sensitivity of ultrasound guided wire-localization was 100.0% (69/69), the sensitivity of nano-carbon staining was 98.6% (68/69), and the sensitivity of combination of the two methods was 97.1% (67/69). The specificity of ultrasound guided wire-localization was 3.3% (3/90), the specificity of nano-carbon staining was 2.2% (2/90), and the specificity of combination of the two methods was 5.6% (5/90). With the count of SLN as the unit, the combination of the two methods had the highest diagnostic efficiency in detecting SLN, and the difference was statistical significant (χ² = 34.31, P < 0.001). Conclusions Ultrasound guided wire-localization and nano-carbon staining are safe and accessible methods for detecting SLN. It provides a precise treatment for early breast cancer, and it can protect medical staff from radiation, which is expected to be the best method for detection of SLN in breast cancer.

We constructed recombinant adenoviruses expressing miR-29b2c (Ad-miR29b2c), and analyzed their effects on the proliferation and migration of HGC-27 and MGC-803 cells. miR-29b2c gene was amplified by PCR from genomic DNA and cloned into the pAdTrack-CMV vector to create the shuttle plasmid pAdT-29b2c. The recombinant plasmid was verified by restriction enzyme digestion and sequencing. The linearized shuttle vector was mixed with an adenoviral backbone plasmid (pAdEasy-1), followed by cotransformation into competent BJ5183 cells to generate the recombinant plasmid pAd-miR-29b2c. Finally, recombinant adenoviral vectors were generated by transfecting the recombinant plasmid into 293A packaging cell line. HGC-27 and MGC-803 cells were infected with the recombinant adenoviruses expressing pAd-miR-29b2c, then MTT and wound-healing assay were used to analyze the effects of pAd-miR-29b2c on the proliferation and migration of HGC-27 and MGC-803 cells. The miR-29b and miR-29c levels were significantly increased in HGC-27 cells after infected with pAd-miR-29b2c. MTT and wound-healing analysis also revealed a significant decrease in proliferation and migration of HGC-27 and MGC-803 cells compared to the control Ad-GFP-infected cells. Furthermore, western blotting results demonstrated that the protein expression level of δ-catenin was reduced in pAd-miR-29b2c transfected HGC-27 and MGC-803 cells. Taken together, the recombinant adenoviral vector was generated, and it can significantly inhibit the proliferation and migration of HGC-27 and MGC-803 cells.

Objective To reduce the incidence rate of hospital-wide indwelling needle catheter blockage through a nursing project.Methods We set up a nursing project team,conducted a cross-sectional survey to know the using status of hospital-wide catheter,and analyzed the reasons of catheter blockage by root cause analysis method. "Reducing the incidence rate of hospital-wide indwelling needle catheter blockage" was established as the theme;improvement activities of nursing project were planned and developed for six months;and improvement methods were developed and implemented.Results The incidence rate of hospital-wide indwelling needle catheter blockage reduced to 3.6% from 8.1% (χ2=752.177,P<0.01),which reaches the goal of controlling the incidence rate of catheter blockage below 4%,and continuously decreases month by month. After implementation,the score of knowledge questionnaire about indwelling needle catheter blockage of nurses in the wards increased from (78.23±7.14) to (96.4±1.48) (t=54.735,P<0.01).Conclusions The nursing project obviously improves the degree of mastering the knowledge about preventing blockage,and reduces the incidence rate of hospital-wide indwelling needle catheter blockage.

Objective To analyze the distribution of multiple myeloma (MM) literature in Chinese periodicals and to find out the core periodicals about MM.Methods Periodical literature that involved with MM in the article titles from January 2008 to December 2014 on the Wanfang medical database was collected and searched.Bibliometric method was used to analyze journal date and journal distribution.Results 456categories of periodicals had published literature about MM.The number of articles about MM published in the top 10 journals was more than 30,accounting for 27.00 ％ of all published MM literature,which were highly effective core journals.The number of articles about MM published in the top 3 journals was more than 100,accounting for 15.59 ％ of all published MM literature.The number in Journal of Leukemia & Lymphoma had reached 187,accounting for 6.43 ％ of the total.Conclusions The core journals about MM provide the key approach to help MM medical personnel to select useful information efficiently.Furthermore,the core journals are not only the significant gist for authors who plan to contribute their articles to relevant MM periodicals,but also the necessary reference tools for medicine workers who engage in basic and clinical research and teaching.