This study analyzed the epidemiological characteristics and strain genotypic distribution of varicella in Fengtai District, Beijing, utilizing population-based surveillance data from March 2024 to February 2025 combined with laboratory nucleic acid detection and genotyping.We reported 522 varicella cases with male predominance (282 cases, 54.41%) and a majority aged >15 years (358 cases, 68.58%).A bimodal incidence pattern peaked in May and November, while 161 breakthrough infections (30.8%) occurred predominantly among students aged 6-20 years.The multivariate logistic regression model analysis showed that, compared to the 0-5 years age group, individuals aged 6-14 years ( OR: 2.729, 95% CI: 1.083-6.88), 15-20 years ( OR: 2.495, 95% CI: 1.158-5.378), and>20 years ( OR: 5.382, 95% CI: 2.478-11.689) exhibited progressively higher odds of oderate-to-severe rash; females demonstrated a lower risk compared to males ( OR: 0.485, 95% CI: 0.286-0.822); regarding vaccination status, recipients of one vaccine dose ( OR: 0.301, 95% CI: 0.161-0.564) and two doses ( OR: 0.203, 95% CI: 0.086-0.48) showed significantly reduced risks relative to unvaccinated individuals. Genotyping of 174 specimens identified 161 VZV-positive samples, with successful ORF22/38/62 sequencing in 142 samples confirming Clade 2 predominance (141 strains, 99.3%) and one Clade 5 strain; local isolates exhibited high vaccine-strain homology (ORF22 nucleotide:99.5%-100%, amino acid:99.3%-100%) with mutation sites partially overlapping other Chinese regions.

This study analyzed the epidemiological characteristics and strain genotypic distribution of varicella in Fengtai District, Beijing, utilizing population-based surveillance data from March 2024 to February 2025 combined with laboratory nucleic acid detection and genotyping.We reported 522 varicella cases with male predominance (282 cases, 54.41%) and a majority aged >15 years (358 cases, 68.58%).A bimodal incidence pattern peaked in May and November, while 161 breakthrough infections (30.8%) occurred predominantly among students aged 6-20 years.The multivariate logistic regression model analysis showed that, compared to the 0-5 years age group, individuals aged 6-14 years ( OR: 2.729, 95% CI: 1.083-6.88), 15-20 years ( OR: 2.495, 95% CI: 1.158-5.378), and>20 years ( OR: 5.382, 95% CI: 2.478-11.689) exhibited progressively higher odds of oderate-to-severe rash; females demonstrated a lower risk compared to males ( OR: 0.485, 95% CI: 0.286-0.822); regarding vaccination status, recipients of one vaccine dose ( OR: 0.301, 95% CI: 0.161-0.564) and two doses ( OR: 0.203, 95% CI: 0.086-0.48) showed significantly reduced risks relative to unvaccinated individuals. Genotyping of 174 specimens identified 161 VZV-positive samples, with successful ORF22/38/62 sequencing in 142 samples confirming Clade 2 predominance (141 strains, 99.3%) and one Clade 5 strain; local isolates exhibited high vaccine-strain homology (ORF22 nucleotide:99.5%-100%, amino acid:99.3%-100%) with mutation sites partially overlapping other Chinese regions.

Objectives:To investigate the relationship between visceral adipose index and glomerular filtration rate in patients with type 2 diabetes mellitus.Methods:A total of 1 036 patients with type 2 diabetes mellitus who received treatment in The Second Affiliated Hospital of Xi'an Jiaotong University from May 2017 to May 2018 were included in this study. The visceral adipose index was detected using a bioresistance assay. These patients were divided into four groups using the quartile method: Visceral adipose index < 8.10 (q1 group, n = 246), 9.60 > visceral adipose index ≥ 8.10 (q2 group, n = 64), 11.10 > visceral adipose index ≥ 9.60 (q3 group, n = 423), visceral adipose index ≥ 11.10 (q4 group, n = 233). One-way analysis of variance was performed to compare the differences among groups. Partial correlation and multiple regression were used to analyze the correlation between body mass index, waist circumference, waist-to-height ratio, waist-to-hip ratio, body fat content, visceral adipose index, and urinary microalbumin and glomerular filtration rate. Results:With the increase in the visceral adipose index, the glomerular filtration rate gradually decreased. The glomerular filtration rate in the q1, q2, q3, q4 groups was (112.19 ± 31.74) mL·min -1·1.73 m -2, (106.14 ± 28.26) mL·min -1·1.73 m -2, (104.73 ± 23.63) mL·min -1·1.73 m -2, (103.40 ± 27.51) mL·min -1·1.73 m -2, respectively. In the female group, with the increase in visceral adipose index, the glomerular filtration rate decreased gradually. After controlling for age, sex, diabetes, and hypertension, the visceral adipose index was significantly correlated with the glomerular filtration rate ( r = -0.10, P < 0.001). Multiple regression analysis showed that visceral adipose index and waist-to-height ratio were closely related to glomerular filtration rate ( F = 6.00, P < 0.001). Conclusion:With the increase of visceral adipose index, body mass index, waist circumference, waist-to-height ratio, waist-to-hip ratio, body fat content, and urinary microalbumin increased gradually. When the visceral adipose index is greater than 9.60, the glomerular filtration rate is significantly decreased. Therefore, it is suggested to adopt various methods to evaluate obesity in clinical work, and visceral fat index should be paid more attention, especially when the visceral fat index is greater than 9.60.

Background::Many studies have emphasized that selective resection of epileptic lesions in temoral lobe is associated with better preservation of cognition function; whether this applies to patients with refractory mesial temporal lobe epilepsy (MTLE) remains unknown. The objective of this study was to evaluate changes in cognitive functions, mood status, and quality of life after anterior temporal lobectomy in patients with refractory MTLE.Methods::This single-arm cohort study assessed cognitive function, mood status, and quality of life, as well as electroencephalography findings, in patients with refractory MTLE who underwent anterior temporal lobectomy at Xuanwu Hospital from January 2018 to March 2019. Pre- and post-operative characteristics were compared to evaluate the effects of surgery.Results::Anterior temporal lobectomy significantly reduced the frequencies of epileptiform discharges. The overall success rate of surgery was acceptable. Anterior temporal lobectomy did not result in significant changes in overall cognitive functions (P > 0.05), although changes in certain domains, including visuospatial ability, executive ability, and abstract thinking, were detected. Anterior temporal lobectomy resulted in improvements in anxiety and depression symptoms and quality of life. Conclusions::Anterior temporal lobectomy reduced epileptiform discharges and incidence of post-operative seizures as well as resulted in improved mood status and quality of life without causing significant changes in cognitive function.

Objective:To explore the association between H3K18 acetylation (H3K18ac) and hepatic injury induced by arsenic in rats, which might to provide a new target for the study of mechanism and intervention of hepatic injury induced by arsenic.Methods:Twenty-four healthy Wistar rats, half male and half female, were selected and divided into control group, low, medium and high arsenic dose groups according to body weight (80-100 g) by random number table method, 6 rats in each group. The control group was given deionized water by gavage, and the low, medium and high arsenic dose groups were given 2.00 g/L sodium arsenite (NaAsO 2) by gavage according to their body weight for 6 days every week, the concentrations of NaAsO 2 in the low, medium and high arsenic dose groups were 2.5, 5.0 and 10.0 mg/kg·bw, respectively. After 4 months treatment, liver tissue and peripheral blood samples of rats were collected. The content of arsenic in liver was measured by inductively coupled plasma mass spectrometry (ICP-MS). Histone was extracted from the liver of rats by acid extraction, and the level of H3K18ac was measured with enzyme-linked immunesorbent assay (ELISA). The serum total bile acid (TBA) and γ-glutamyl transpeptidase (γ-GT) levels were measured by fully automatic biochemical instrument. Results:The difference of arsenic content in liver among the control group, low, medium and high arsenic dose groups [median (quartile range): 2.41 (1.83, 2.99), 62.64 (56.26, 65.17), 68.81 (62.58, 77.24), 88.48 (74.47, 98.99) μg/g] was statistically significant ( H=18.98, P < 0.01). The arsenic contents in liver in the low, medium and high arsenic dose groups were higher than that of the control group ( P < 0.05), and the arsenic content in liver increased gradually with the increase of arsenic dose ( Ztrend=5.04, P < 0.01). The differences of serum TBA and γ-GT levels among the control group and low, medium and high arsenic dose groups were statistically significant ( F=11.11, 12.26, P < 0.05). The TBA and γ-GT levels increased gradually with the increase of arsenic dose ( Ftrend=32.09, 33.22, P < 0.01). The difference of H3K18ac levels among the control group, low, medium and high arsenic dose groups was statistically significant ( F=4.62, P < 0.05). Compared with the control group, the levels of H3K18ac in the medium and high arsenic dose groups decreased ( P < 0.05), and the level of H3K18ac in liver decreased gradually with the increase of arsenic dose ( Ftrend=12.82, P < 0.01). The results of correlation analysis showed that there were positive correlation between liver arsenic content and the liver function indicators of TBA and γ-GT levels( r=0.631, 0.863, P < 0.01), while the liver arsenic content were negatively correlated with H3K18ac level ( r=- 0.476, P < 0.05). And the level of H3K18ac were negatively correlated with the liver function indicators of TBA and γ-GT levels ( r=- 0.628,-0.544, P < 0.05). The results of the mediating effect analysis showed that the H3K18ac had a partly mediating effect on the effect of arsenic exposure on liver function indicators of TBA and γ-GT, and the proportion of mediating effect on TBA and γ-GT to total effect was 37.10% [95% confidence interval ( CI): 9.71%-92.45%] and 20.05% (95% CI: 2.61%-52.89%), respectively. Conclusion:The level of H3K18ac in the liver of rats is not only respond to arsenic exposure, but is also closely related to hepatic injury induced by arsenic, suggesting that H3K18ac may be used as a new target for the study of liver injury mechanism and intervention of hepatic injury induced by arsenic.

Objective:To investigate the relationship between 25-hydroxy vitamin D [25(OH)D)] in blood and follicular fluid and pregnancy outcome after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). Methods:A prospective cohort study of 201 infertility patients who were treated with IVF/ICSI-ET due to fallopian tube factors in Department of Reproductive Medicine, Xiangya Hospital, Central South University was performed. The cut-off values of 25(OH)D levels in serum and follicular fluid were determined by the method of minimizing P value. The correlations was studied between serum or follicular fluid levels of 25(OH)D and age, body mass index (BMI), past IVF history,endometrial thickness on human chorionic gonadotropin (hCG) injection day, gonadotropin (Gn), progesterone, estradiol, number of retrieved oocytes, and M II oocytes, normal fertilization rate, day 3 high-quality embryo rate, embryo implantation rate, clinical pregnancy rate and early abortion rate. Binary logistic regression analysis of influencing factors of pregnancy outcome was done. Results:1)The serum 25(OH)D level was 16.4(13.1, 20.2) μg/L and the follicular fluid 25(OH)D was 15.3(11.6,20.3) μg/L. There was a significant linear correlation between the two groups ( r=0.78, P<0.001). 2)The clinical pregnancy rate (33.87%) and the embryo implantation rate (25.44%) in the low level group of 25(OH)D in serum were lower than those in the high level group (52.52%, P=0.014; 40.24%, P=0.006). The clinical pregnancy rate (39.39%) and the embryo implantation rate (30.22%) in the low level group of 25(OH)D in follicular fluid were lower than those in the high level group (53.92%, P=0.039; 40.98%, P=0.032). The early abortion rate in the low level group of 25(OH)D in follicular fluid (17.95%) was higher than that in the high level group (7.27%, P=0.002). 3) The results of two classification logistic regression analysis showed that the levels of 25(OH)D in serum and follicular fluid were related to clinical pregnancy outcome ( P=0.016, P=0.020). Conclusion:There was a significant linear correlation between 25(OH)D in serum and that in follicular fluid. The levels of 25(OH)D in serum and follicular fluid were correlated with the pregnancy outcome of IVF/ICSI-ET.

Objective:To investigate the relationship between 25-hydroxy vitamin D [25(OH)D)] in blood and follicular fluid and pregnancy outcome after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). Methods:A prospective cohort study of 201 infertility patients who were treated with IVF/ICSI-ET due to fallopian tube factors in Department of Reproductive Medicine, Xiangya Hospital, Central South University was performed. The cut-off values of 25(OH)D levels in serum and follicular fluid were determined by the method of minimizing P value. The correlations was studied between serum or follicular fluid levels of 25(OH)D and age, body mass index (BMI), past IVF history,endometrial thickness on human chorionic gonadotropin (hCG) injection day, gonadotropin (Gn), progesterone, estradiol, number of retrieved oocytes, and M II oocytes, normal fertilization rate, day 3 high-quality embryo rate, embryo implantation rate, clinical pregnancy rate and early abortion rate. Binary logistic regression analysis of influencing factors of pregnancy outcome was done. Results:1)The serum 25(OH)D level was 16.4(13.1, 20.2) μg/L and the follicular fluid 25(OH)D was 15.3(11.6,20.3) μg/L. There was a significant linear correlation between the two groups ( r=0.78, P<0.001). 2)The clinical pregnancy rate (33.87%) and the embryo implantation rate (25.44%) in the low level group of 25(OH)D in serum were lower than those in the high level group (52.52%, P=0.014; 40.24%, P=0.006). The clinical pregnancy rate (39.39%) and the embryo implantation rate (30.22%) in the low level group of 25(OH)D in follicular fluid were lower than those in the high level group (53.92%, P=0.039; 40.98%, P=0.032). The early abortion rate in the low level group of 25(OH)D in follicular fluid (17.95%) was higher than that in the high level group (7.27%, P=0.002). 3) The results of two classification logistic regression analysis showed that the levels of 25(OH)D in serum and follicular fluid were related to clinical pregnancy outcome ( P=0.016, P=0.020). Conclusion:There was a significant linear correlation between 25(OH)D in serum and that in follicular fluid. The levels of 25(OH)D in serum and follicular fluid were correlated with the pregnancy outcome of IVF/ICSI-ET.

Objective: To evaluate the value of non-invasive prenatal testing (NIPT) in pregnancies with anomaly in prenatal screening. Methods: This was a retrospective study of 2 837 singleton pregnancies who performed NIPT indicated by isolated anomaly in prenatal screening at Guangdong Women and Children Hospital between November 2014 and August 2016. All pregnancies were divided into 3 groups by single indication: advanced maternal age ( AMA, ≥35), abnormal multiples of the median (MoM) in standard screening, increased nuchal translucency thickness (NT, 2.5-3.0 mm). High risk results were verified by prenatal diagnosis. Low risk cases were followed by a 22-26 week anatomical ultrasound examination. All of the cases were followed up and the performance of NIPT for every single indication was evaluated. Results: There were total of 2 837 pregnant women who underwent NIPT. Twenty-five of 2 448 pregnancies indicated by AMA had high risk results, among which 17 were confirmed by invasive genetic testing, except 1 case rejecting prenatal diagnosis. In 351 pregnant women with abnormal MoM, NIPT found 3 cases of sex chromosome aneuploidies (SCA) and 2 of them were validated by invasive prenatal diagnosis. Increased NT group included 38 cases, NIPT found 1 case of trisomy 21 which was consistent with karyotype analysis. For common aneuploidies and SCA, the performance of NIPT in the pregnant women who indicated by AMA, abnormal MoM and increased NT were as the follows: the sensitivity were 17/17, 2/2 and 1/1 respectively, the specificity were 99.7% (2 423/2 431), 99.7% (348/349) and 100%(37/37), the positive predictive value were 68% (17/25), 2/3 and 1/1, the negative predictive value were 100% (2 423/2 423), 100% (348/348) and 100% (38/38), respectively. By follow-up survey, a total of 8 cases of abnormal fetus were recorded in NIPT low-risk women, including 5 cases of termination of pregnancy due to abnormal ultrasound findings, 2 cases of abortion as a result of severe obstetric complications and 1 case of stillbirth. Conclusions To the pregnant women who indicated by advanced maternal age, abnormal MoM and increased NT (2.5-3.0 mm), NIPT had satisfactory performance for common aneuploidies, and also had potential value for SCA, resulting in a significant reduction in diagnostic procedures. However, for NIPT low-risk pregnancies, routine antenatal examination and anatomical ultrasound detection would be highly necessary to avoid missing abnormal fetuses.(Chin J Lab Med, 2018, 41: 509-513)

Objective To study the association between brachial-ankle index (BAI) and severe subclavian artery stenosis.Methods Seventy severe subclavian artery stenosis patients with a stenosis rate ≥70％ admitted to our hospital served as a stenosis group and 70 subclavian artery stenosis-free patients served as a control group in this study.Their BAI,pulse wave upstroke time (UT) and pulse wave form were recorded with a noninvasive automatic peripheral arterial device.The sensitivity,specificity,accuracy,positive and negative prediction value of BAI in diagnosis of subclavian artery stenosis were calculated.Results The BAI of stenosis-involved extrmities was significantly lower while the pulse wave UT was significantly longer in stenosis group than in control group (0.6±0.1 vs 0.9±0.1 and 0.9±0.1,261.0±11.8 vs 188.0±12.1 ms and 186.0±12.6 ms,P＜0.01).The low obtuse pulse wave form and low plain pulse wave form were detected respectively in stenosis involved extrmities of 58 patients (82.9％) and 11 patients (15.7％) of stenosis group.The sensitivity,specifity,accuracy,positive and negative prediction value of BAI in diagnosis of severe subclavian artery stenosis were 44.3％,92.9％,68.6％,86.1％ and 62.5％ respectively.Conclusion The specifity,positive prediction value and accuracy of BAI＜ 0.70 are rather high in diagnosis of severe subclavian artery stenosis.

Objective To study the effects of Qingzao Runfei Huazhuo Xingxue decoction (QRHXD) on inflammatory reaction and histopathology in mice with PM2.5-induced pulmonary injury, and to approach the possible mechanism of prevention and treatment of traditional Chinese medicine on lung injury induced by haze.Methods Fifty healthy male C57BL/6 mice were randomly divided into five groups (n = 10): namely control, PM2.5, PM2.5+ low-, moderate-, and high-dose groups. The PM2.5 suspensions at a dosage of 40 mg/kg was respectively given to mice by the nasal instillation for reproduction of mouse model of lung injury induced by PM2.5, and the mice in control group were given the same volume of normal saline. The mice in PM2.5+ low-, moderate-, and high-dose QRHXD groups were given 15, 25, 50 mL·kg-1·d-1 QRHXD by oral perfusion daily for consecutive 21 days at the next day of model reproduction (the QRHXD included: Pear 75 g,Bulbus Fritillariae Cirrhosae 10 g, Radix Stemonae 8 g,Rhizoma Pinelliae 8 g,Radix Platycodi 6 g, Aster 10 g, Almond 5 g, Lily 6 g, Rhodiola 4 g, Lotus 3 g,Fructrs Liquidambaris 6 g,Radix Paeoniae Rubra 5 g, Semen Cassiae 6 g). The mice in control and PM2.5 groups were given equivalent volume of normal saline respectively. After treatment for 21 days, the mice were sacrificed, and the left lung was harvested for bronchoalveolar lavage, and the bronchoalveolar lavage fluid (BALF) was collected for determination of levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactic dehydrogenase (LDH), and albumin (ALB). The right lung was harvested for histopathology observation under light microscope using hematoxylin and eosine (HE) staining.Results After intranasal instillation of PM2.5 suspension, the levels of ACP, AKP, LDH, and ALB in PM2.5 group were significantly higher than those in control group [ACP (U/L): 3.9±0.4 vs. 1.7±0.3, AKP (U/L): 9.0±1.5 vs. 4.8±0.3, LDH (U/L): 416.7±44.4 vs. 112.5±20.3, ALB (mg/L): 198.7±32.4 vs. 65.8±21.3, all P < 0.05]. Under light microscope, the PM2.5 particles were collected, the alveolar septa were thickened, and the inflammatory cells in the alveolar cavity and pulmonary interstitium were found. On the contrary, after administration of QRHXD, a significant reduction of biochemical indexes was found, which showed a dose-dependent manner. The parameters of PM2.5+ high-dose QRHXD group were significantly lower than those in PM2.5 group [ACP (U/L): 2.1±0.8 vs. 3.9±0.4, AKP (U/L): 5.3±1.4 vs. 9.0±1.5, LDH (U/L): 146.6±29.8 vs. 416.7±44.4, ALB (mg/L): 88.5±26.7 vs. 198.7±32.4, all P < 0.05]. At the same time, the pathological changes in lung tissue were better with the increase of the dose.Conclusions QRHXD can reduce the pulmonary inflammatory response and tissue damage caused by PM2.5, with the increase concentration of Chinese medicine, and the effect is more obvious. This may be related to the immune response of the human body to regulate inflammatory mediators, which provide basis for the treatment of pulmonary injury induced by PM2.5.