Burkholderia cepacia complex (Bcc) is an emerging nosocomial pathogen, with violet-pigmented strains representing a rare and possibly neglected but clinically important subset. We report a case of a 62-year-old man with end-stage renal disease on dialysis who presented with fever and cough. Blood cultures from central and peripheral lines grew violet-pigmented, non-lactose fermenting colonies on MacConkey agar, later identified as Burkholderia contaminans sequence type 922 (ST922) via a multimodal approach. Initial phenotypic methods (VITEK-2, API20NE and MALDI-ToF) and 16S rDNA sequencing were inconclusive; however, multilocus sequence typing confirmed ST922, a strain previously reported in India and associated with nosocomial outbreaks. The isolate was susceptible to ceftazidime, trimethoprim/sulfamethoxazole, minocycline, and meropenem. The patient responded well to intravenous ceftazidime, with subsequent blood cultures yielding no growth. Notably, antimicrobial susceptibility varied geographically, with some ST922 isolates resistant to third-generation cephalosporins. This case illustrates the diagnostic challenges in Bcc speciation and highlights the value of molecular tools such as MLST. The detection of this strain in Malaysia raises concerns about global dissemination, potentially via contaminated medical devices or water systems. Early recognition of violet-pigmented Bcc in resource-limited settings can facilitate prompt, targeted treatment. This report underscores the need for environmental surveillance and antimicrobial stewardship to prevent spread, while reinforcing Bcc’s ecological adaptability and clinical significance, especially in immunocompromised individuals.

Canine Enteric Coronavirus (CCoV) is one of the major enteric pathogen affecting dogs. This study aims to investigate the molecular prevalence, phylogenetic analysis, associated risk factors, and haemato-biochemical alterations in Canine Coronavirus in dogs in district Lahore, Pakistan. 450 fecal samples were collected from symptomatic dogs originating from various pet-clinics and kennels during 2018-2019. Samples were initially analyzed by sandwich lateral flow immunochromatographic assay and then further processed by RT-PCR (reverse transcriptase polymerase chain reaction) targeting the M gene followed by sequencing. RT-PCR based positive (n=20) and negative (n=20) dogs were samples for their blood for the haemato-biochemical analysis. A questionnaire was used to collect data from pet owners, in order to analyze the data for risk factors analysis by chi square test on SPSS. The prevalence of CCoV was 35.1%, and 23.8 % through Sandwich lateral flow immunochromatographic and RT-PCR respectively. Various risk factors like breed, age, sex, vomiting, diarrhea, sample source, body size, cohabitation with other animals, living environment, food, deworming history, contact with other animals or birds feces, and season were significantly associated with CCoV. The CCoV identified in Pakistan were 98% similar with the isolates from China (KT 192675, 1), South Korea (HM 130573, 1), Brazil (GU 300134, 1), Colombia (MH 717721, 1), United Kingdom (JX 082356, 1) and Tunisia (KX156806). Haematobiochemical alterations in CCoV affected dogs revealed anaemia, leucopenia, lymphopenia, neutrophilia, and decreased packed cell volume, and a significant increase in alkaline phosphate and alanine transaminase. It is concluded that infection with canine coronavirus appears widespread among dog populations in district Lahore, Pakistan. This study is the first report regarding the molecular detection and sequence analysis of CCoV in Pakistan.