2.Selection and validation of reference genes for quantitative real-time PCR analysis in Tujia medicine Xuetong.
Qian XIAO ; Chen-Si TAN ; Jiang ZENG ; Yuan-Shu XU ; Tian-Hao FU ; Lu-Yun NING ; Wei WANG
China Journal of Chinese Materia Medica 2025;50(3):682-692
Tujia ethnic group medicine Xuetong is derived from Kadsura heteroclita, the stem of which has the medicinal value for anti-rheumatoid arthritis, liver protection, anti-tumor, anti-oxidation effects, and has been widely used in Hunan and Guangdong in China. The selection of reliable and stable reference genes is the basis for subsequent molecular research on K. heteroclita. In this study, GAPDH, TUA, Actin, UBQ, EF-1α, 18S-rRNA, CYP, UBC, TUB, H2A, and RPL were selected as candidate reference genes in Kadsura heteroclita. The gene expression levels of the 11 candidate reference genes of K. heteroclita in its 6 different parts(stem-inside of the cambium, stem-outside of the cambium, fruit, flower, root, and leaf) and under different intervention conditions [drought stress, salt stress, and methyl jasmonate(MeJA) treatment] were detected by quantitative real-time polymerase chain reaction(qRT-PCR). The expression stability of the 11 candidate reference genes was comprehensively analyzed and evaluated by geNorm, NormFinder, ΔCT algorithm, and RefFinder software. The results showed that the expression of UBC and RPL was relatively stable in 6 different parts, and UBC and GAPDH genes were relatively stable under different intervention conditions. To verify the reliability of reference genes for K. heteroclita, this study further examined the relative expression levels of KhFPS, KhIDI, KhCAS, KhSQE, KhSQS, KhSQS-2, KhHMGS, KhHMGR, KhMVD, KhMVK, KhDXR, KhDXS, KhPMVK, and KhGGPS in different parts and under different intervention conditions, which might relate to the synthesis of the main component(Xuetongsu) of K. heteroclita. The results showed that with UBC and RPL or UBC and GAPDH as the reference genes, the expression trends of these 14 genes were basically consistent in different parts or under different intervention conditions for K. heteroclita. In conclusion, UBC can be used as a reference gene of K. heteroclita for its different parts and different intervention conditions, which lays a foundation for further research on the biosynthetic pathway of main components in K. heteroclita.
Real-Time Polymerase Chain Reaction/methods*
;
Reference Standards
;
Gene Expression Regulation, Plant
;
Gene Expression Profiling
;
Plant Proteins/metabolism*
;
Drugs, Chinese Herbal
3.Revision and prospect of "Standards for indoor air quality(GB/T 18883-2022)" in China.
Chinese Journal of Preventive Medicine 2023;57(11):1725-1728
The formulation and revision of the detection methods of indoor air quality standards is an important, rigorous and delicate endeavor. The standards for indoor air quality (GB/T 18883-2022) were issued by the State Administration of Market Regulation and the Standardization Administration on July 11, 2022, and implemented on February 1, 2023 by replacing indoor air quality standards (GB/T 18883-2002). The revised standard specifies hygienic requirements for physical, chemical, biological and radioactive indicators in indoor air and the corresponding test methods. This article interpreted the revision background, drafting principles, main indicators and methods, as well as the revision basis of the standards. Recommendations for the implementation of the standards are also proposed.
Humans
;
Air Pollution, Indoor/prevention & control*
;
Environmental Monitoring
;
Reference Standards
;
China
;
Air Pollutants/analysis*
4.Study on formulation and revision of detection methods of "Standards for indoor air quality (GB/T 18883-2022)" in China.
Hai Jing ZHANG ; Yi Fu LU ; Qin WANG ; Yan Wei YANG ; Yun Pu LI ; Yun Yun WU ; Cheng DING ; Jun Rui CHANG ; Ying ZHU ; Dong Qun XU
Chinese Journal of Preventive Medicine 2023;57(11):1777-1781
The formulation and revision of the detection methods of indoor air quality standards is an important, rigorous and delicate endeavor. This paper introduced the formulation and revision of the detection methods of the standards for indoor air quality (GB/T 18883-2022), focusing on the revision process, revision principles, main adjustments and technical points of some key indicators to facilitate users to better understand and apply the detection methods in standards for indoor air quality (GB/T 18883-2022).
Humans
;
Air Pollution, Indoor
;
China
;
Reference Standards
;
Air Pollutants/analysis*
5.Appropriate range of body mass index and body weight management guidelines for Chinese oldest old (T/CNSS 021-2023).
Chinese Journal of Epidemiology 2023;44(9):1335-1337
Body mass index (BMI) is widely recognized as an important indicator of physical health. However, the current BMI standard and guidelines primarily focus on children and general adults, neglecting the specific needs of the oldest old aged 80 years or older. To address this gap, this guideline aims to provide recommendations on the appropriate range of BMI and weight management for the oldest old in China, including age calculation, defining the appropriate BMI range, BMI measurement indicators, and weight management strategies. It is intended to be utilized by medical and health organizations at all levels, as well as medical and nursing service institutions, to evaluate the BMI and manage the weight of the oldest old population.
Aged, 80 and over
;
Humans
;
Asian People
;
Body Mass Index
;
Body Weight
;
China
;
East Asian People
;
Reference Values
;
Health Promotion/standards*
6.Revision and prospect of "Standards for indoor air quality(GB/T 18883-2022)" in China.
Chinese Journal of Preventive Medicine 2023;57(11):1725-1728
The formulation and revision of the detection methods of indoor air quality standards is an important, rigorous and delicate endeavor. The standards for indoor air quality (GB/T 18883-2022) were issued by the State Administration of Market Regulation and the Standardization Administration on July 11, 2022, and implemented on February 1, 2023 by replacing indoor air quality standards (GB/T 18883-2002). The revised standard specifies hygienic requirements for physical, chemical, biological and radioactive indicators in indoor air and the corresponding test methods. This article interpreted the revision background, drafting principles, main indicators and methods, as well as the revision basis of the standards. Recommendations for the implementation of the standards are also proposed.
Humans
;
Air Pollution, Indoor/prevention & control*
;
Environmental Monitoring
;
Reference Standards
;
China
;
Air Pollutants/analysis*
7.Study on formulation and revision of detection methods of "Standards for indoor air quality (GB/T 18883-2022)" in China.
Hai Jing ZHANG ; Yi Fu LU ; Qin WANG ; Yan Wei YANG ; Yun Pu LI ; Yun Yun WU ; Cheng DING ; Jun Rui CHANG ; Ying ZHU ; Dong Qun XU
Chinese Journal of Preventive Medicine 2023;57(11):1777-1781
The formulation and revision of the detection methods of indoor air quality standards is an important, rigorous and delicate endeavor. This paper introduced the formulation and revision of the detection methods of the standards for indoor air quality (GB/T 18883-2022), focusing on the revision process, revision principles, main adjustments and technical points of some key indicators to facilitate users to better understand and apply the detection methods in standards for indoor air quality (GB/T 18883-2022).
Humans
;
Air Pollution, Indoor
;
China
;
Reference Standards
;
Air Pollutants/analysis*
9.Revision and prospect of the "Standards for Drinking Water Quality (GB5749-2022)" in China.
Chinese Journal of Preventive Medicine 2023;57(6):801-805
The revision of the national standards for drinking water quality is an important, rigorous and delicate endeavor. The paper introduced the revision of this standard, emphasizing the revision principle, overall technical considerations, and revision contents. Recommendations were also proposed for the implementation of this standard.
Humans
;
Drinking Water
;
Water Quality
;
Reference Standards
;
China
;
Water Pollutants, Chemical/analysis*
;
Water Supply
10.Methodological study on the establishment of limit values in the Standards of Drinking Water Quality.
Lan ZHANG ; Xiao Yuan YAO ; Bi Xiong YE ; Jia Yi HAN ; Sheng Hua GAO
Chinese Journal of Preventive Medicine 2023;57(6):839-843
The establishment of limit values for standards of drinking water quality is an important and complex process. This study systematically introduced the methodology of the establishment of standard limit values for drinking water quality and elaborated on the workflow of setting limit values of water quality indicators, principles and methods of selecting water quality indicators, derivation of safety reference values, and establishment of limit values. It also aimed to provide reference and support for the future revision of relevant standards.
Humans
;
Water Supply
;
Drinking Water
;
Reference Standards
;
Water Quality
;
Water Pollutants, Chemical/analysis*


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