Objective To clarify the origin,composition,source of medicinal ingredients,dosage and efficacy of the classic prescription Tibetan medicine"Siwei-Shiliu Powder",and provide literature evidence and scientific basis for its modern clinical application and drug development.Methods Based on ancient Tibetan medical literature and combined with the research methods of bibliometrics,literature screening was conducted,the prescription origin,ingredient composition,dosage,main therapeutic effects and modern research literature of"Siwei-Shiliu Powder"were investigated and analyzed.Results"Siwei-Shiliu Powder"was mentioned in 27 ancient Tibetan medical books and there were 3 different prescriptions with the same name.The most commonly used"Siwei-Shiliu Powder"consists of pomegranate,cinnamon,cardamom and Piper longum.Its prescription can be traced back to the"Biji-Huangjing"of the Tubo period and has been passed down to the present through the"Four Medical Codes".The formula ratio of"Siwei-Shiliu Powder"was 2∶1∶1∶1,which meant 2 portions of pomegranate(Xiao,about 6.96 g),1 portion each of cinnamon,white cardamom and dried ginger(Xiao,about 6.96 g).It had the functions of nourishing the stomach and generating fire,stimulating appetite and treating cold,and was used for separating clear from turbid,indigestion,loss of appetite and undigested food.It could be appropriately applied in the initial treatment of latent heat diseases.Conclusion This study confirms the formula's earliest record in"Biji Huangjing"during the Tibetan Empire period and its transmission through"Four Medical Codes",establishing it as a core classical prescription of classical Tibetan medicine formulas.The issue of confusion between different formulas with the same name has been clarified,and the mainstream composition has been established as pomegranate,cinnamon,white cardamom,and long pepper(in a ratio of 2∶1∶1∶1),which serves as the universal standard in Tibetan medicine.(2∶1∶1∶1).The scientific essence of the"Siwei-Shiliu Powder"—its ability to"warm the stomach and promote digestion,dispel cold and resolve stagnation"—has been revealed.This is achieved by regulating the gastrointestinal microbiome and enhancing digestive enzyme activity,thereby fulfilling its core function of"separating the clear from the turbid."This provides a pharmacological basis for treating indigestion(Péilóng cold disorder).Furthermore,modern research has confirmed its potential for early intervention in latent heat diseases,broadening the scope for research and development of Tibetan medicine in preventing and treating metabolic diseases such as hyperlipidemia.

Objective To clarify the origin,composition,source of medicinal ingredients,dosage and efficacy of the classic prescription Tibetan medicine"Siwei-Shiliu Powder",and provide literature evidence and scientific basis for its modern clinical application and drug development.Methods Based on ancient Tibetan medical literature and combined with the research methods of bibliometrics,literature screening was conducted,the prescription origin,ingredient composition,dosage,main therapeutic effects and modern research literature of"Siwei-Shiliu Powder"were investigated and analyzed.Results"Siwei-Shiliu Powder"was mentioned in 27 ancient Tibetan medical books and there were 3 different prescriptions with the same name.The most commonly used"Siwei-Shiliu Powder"consists of pomegranate,cinnamon,cardamom and Piper longum.Its prescription can be traced back to the"Biji-Huangjing"of the Tubo period and has been passed down to the present through the"Four Medical Codes".The formula ratio of"Siwei-Shiliu Powder"was 2∶1∶1∶1,which meant 2 portions of pomegranate(Xiao,about 6.96 g),1 portion each of cinnamon,white cardamom and dried ginger(Xiao,about 6.96 g).It had the functions of nourishing the stomach and generating fire,stimulating appetite and treating cold,and was used for separating clear from turbid,indigestion,loss of appetite and undigested food.It could be appropriately applied in the initial treatment of latent heat diseases.Conclusion This study confirms the formula's earliest record in"Biji Huangjing"during the Tibetan Empire period and its transmission through"Four Medical Codes",establishing it as a core classical prescription of classical Tibetan medicine formulas.The issue of confusion between different formulas with the same name has been clarified,and the mainstream composition has been established as pomegranate,cinnamon,white cardamom,and long pepper(in a ratio of 2∶1∶1∶1),which serves as the universal standard in Tibetan medicine.(2∶1∶1∶1).The scientific essence of the"Siwei-Shiliu Powder"—its ability to"warm the stomach and promote digestion,dispel cold and resolve stagnation"—has been revealed.This is achieved by regulating the gastrointestinal microbiome and enhancing digestive enzyme activity,thereby fulfilling its core function of"separating the clear from the turbid."This provides a pharmacological basis for treating indigestion(Péilóng cold disorder).Furthermore,modern research has confirmed its potential for early intervention in latent heat diseases,broadening the scope for research and development of Tibetan medicine in preventing and treating metabolic diseases such as hyperlipidemia.

OBJECTIVE：To investigate t he p otential mechanism of Tibetan medicine Wuwei shexiang pills in the treatment of “Grum bu ”disease（i.e. rheumatoid arthritis ）. METHODS ：Referring to Sibu Yidian ，Drug Standard of the Ministry of Public Health（Tibetan Medicine ），Jingzhubencao，the constituent ，dose and flavor of Wuwei shexiang pills were collected ；vector structure model was built. From the aspects of six tastes ，three tastes after digestion and seventeen effects ，the properties of the formulation were analyzed ；the“formulation-drug property-disease ”network for the treatment of “Grum bu ”disease was constructed by using Gephi 0.9.2 complex network software. The effective components of Wuwei shexiang pills were screened by using the pharmacology analysis platform of TCM system and the database of organic small molecule biological activity. The target proteins corresponding to the active components were predicted by using BATMAN-TCM network pharmacology research platform ； the target proteins of “Grum bu ”disease were searched by ETCM database. On the basis of screening the common targets of the two，David 6.8 bioinformatics resource database was adopted to conduct gene ontology （GO） analysis and KEGG pathway enrichment analysis ；the network of Wuwei shexiang pills- “Grum bu ”- target-pathway was constructed by using Cytoscape 3.7.0 software，and the network topology analysis was carried out to screen the core targets. Using Glide score as evaluation index ， Maestro Version 11.1.011 software was used for molecular docking of above core targets with effective components of Wuwei shexiang pills. RESULTS & CONCLUSIONS ：Wuwei shexiang pills contains myrobalan ，Aconitum pendulum ，Aucklandia lappa ， Acorus calamus and artificial moschus ，and the highest dosage was myrobalan. The six tastes of this formulation were mainly bitter and sweet ；the three tastes after digestion were mainly bitter ；the seventeen effects were mainly blunt ，cool and heavy ，which were mainly used to treat twenty characteristics such as acute ，hot and light. In “formulation-property-disease”network，higher values of edge weight between seventeen effects and twenty characteristics were cooling effect-heat ， blunt effect-sharpness ， heavy effect-light，dilute effect-odor （edge weight values ≥430），etc. A total of 2 306 potential targets of effective components of Wuwei shexiang pills ，211 targets related to “Grum bu ”disease，32 common targets and 29 corresponding effective components were obtained. The results of GO analysis showed that 52 relative results were predicted ，the common targets mainly located in the extracellular area ，nucleus and other parts ，mainly including biological processes and molecular functions such as immune response，inflammatory response ，cytokine activity. The results of KEGG enrichment were significant in 31 pathways（P＜0.05）， involving TNF signaling pathway ，cancer pathway and other signaling pathways. There were 94 active components ，targets and pathway nodes in the network of Wuwei shexiang pills- “Grum bu ”-target-pathway，and 460 edges；TNF，FAS，IL6，IL10， FASLG，PTGS2 and IL 1B were the core targets of the network ， connected with effective components such as quinic acid ， thymol，dehydroepiandrosterone，norcaxone by Van Der Waals force ，hydrogen bond and hydrophobic interaction ，Pi-cation bond and other bonds ，so as to play a role in the treatment of “Grum bu ”disease.