BACKGROUND:Novel biomaterials not only provide the necessary mechanical support but also promote cell proliferation and differentiation,inducing bone regeneration,thereby improving therapeutic outcomes and offering new perspectives and methods for the development of bone regenerative technologies.OBJECTIVE:To visualize the research status and development prospects of biomaterials in the field of bone regeneration through bibliometric methods.METHODS:A total of 500 of the most influential papers on bone regeneration and biomaterials were selected from the Web of Science Core Collection database from inception to September 24,2024.VOSviewer and CiteSpace tools were used for in-depth bibliometric visualization analysis to reveal the research trends and core literature in this field.RESULTS AND CONCLUSION:Among the selected 500 papers,China and the United States were in a leading position in terms of the number of published papers and citation rates,with ChangJiang being the most prolific author and the journal Acta Biomaterialia publishing the most articles.Bone regeneration and material science is an interdisciplinary research field,covering various disciplines including materials science,biomedical engineering,cell biology,and molecular biology.Research on bone repair materials is shifting from traditional bioinert materials to bioactive materials,which not only provide mechanical support but also promote cell proliferation and differentiation,inducing bone regeneration.Synthetic bone repair materials are gradually replacing traditional materials,becoming the preferred choice for clinical bone graft surgery due to their abundant sources,adjustable physicochemical properties,and lower risks of immune rejection and disease transmission.Researchers are continuously improving the biocompatibility,biomimetic properties,osteoconductivity,and osteoinductivity of these materials to make them closer to natural bone.The frontiers are mainly focused on bioactive ceramics,3D printing,hydrogels,chitosan,hydroxyapatite,and other materials.New materials play a vital role in the field of bone regeneration.With the continuous advancement of materials science and technology,the application prospects of these new materials in the field of bone regeneration are very broad,and they are expected to provide more effective and personalized treatment options for bone defect treatment.

BACKGROUND:Novel biomaterials not only provide the necessary mechanical support but also promote cell proliferation and differentiation,inducing bone regeneration,thereby improving therapeutic outcomes and offering new perspectives and methods for the development of bone regenerative technologies.OBJECTIVE:To visualize the research status and development prospects of biomaterials in the field of bone regeneration through bibliometric methods.METHODS:A total of 500 of the most influential papers on bone regeneration and biomaterials were selected from the Web of Science Core Collection database from inception to September 24,2024.VOSviewer and CiteSpace tools were used for in-depth bibliometric visualization analysis to reveal the research trends and core literature in this field.RESULTS AND CONCLUSION:Among the selected 500 papers,China and the United States were in a leading position in terms of the number of published papers and citation rates,with ChangJiang being the most prolific author and the journal Acta Biomaterialia publishing the most articles.Bone regeneration and material science is an interdisciplinary research field,covering various disciplines including materials science,biomedical engineering,cell biology,and molecular biology.Research on bone repair materials is shifting from traditional bioinert materials to bioactive materials,which not only provide mechanical support but also promote cell proliferation and differentiation,inducing bone regeneration.Synthetic bone repair materials are gradually replacing traditional materials,becoming the preferred choice for clinical bone graft surgery due to their abundant sources,adjustable physicochemical properties,and lower risks of immune rejection and disease transmission.Researchers are continuously improving the biocompatibility,biomimetic properties,osteoconductivity,and osteoinductivity of these materials to make them closer to natural bone.The frontiers are mainly focused on bioactive ceramics,3D printing,hydrogels,chitosan,hydroxyapatite,and other materials.New materials play a vital role in the field of bone regeneration.With the continuous advancement of materials science and technology,the application prospects of these new materials in the field of bone regeneration are very broad,and they are expected to provide more effective and personalized treatment options for bone defect treatment.

Objective:Peripheral nerve injury(PNI)is the most common clinical condition.In this study,the current hotspot and future trends of the therapeutic role of microRNA(miRNA)in posterior axon regeneration were evaluated,through bibliometric analysis of peripheral nerves,to provide a reliable reference for later related studies.Methods:Published data from 2009 to 2024 were collected from the Web of Science database by key words TS=mi-croRNA OR TS=miRNA AND TS=peripheral nerve injury OR TS=microRNA OR TS=miRNA AND TS=axonal re-generation screening.The visualization analysis software CiteSpace was used to evaluate the author,country,keywords,outbreak words,institutions,etc.Results:A total of 301 documents were retrieved from the Web of Science database,and 288 documents finally fit the study post eliminating and screening.The 288 articles used in this study have a total of 1513 authors from 34 countries,and 1513 authors from 358 institutions published in 148 journals,citing 13941 articles from 2038 journals.In addition,this study lists the top 5 authors of the annual publication,national distribution,5 arti-cles with the most cited times and the top ten institutions of articles.Moreover,among them,China is the country with the largest number of articles,but there is still a significant gap in the quality of articles when compared with European and American regions.The analysis of institutions found that domestic institutions account for the largest proportion of the research in this field,with close mutual cooperation,but lack of cooperation with foreign institutions.The most cited article in research is"Let-7 microRNAs Regenerate Peripheral Nerve Regeneration by Targeting Nerve Growth Factor"which was cited 34 times.The institution with the largest publication volume is 31 articles from Nantong University.Conclusion:Although the research on therapeutic role of tiny RNA in axon regeneration after peripheral nerve injury started late,the number of papers is relatively small,but it has great potential.As the most popular topic today,tiny RNA has been inuetigated regarding its mechanistic action in the fields of nerve inflammation,nerve cell repair and re-generation,neuroprotection and functional recovery.With the investment in scientific research in this field,more high-quality articles will be published in excellent journals at home and abroad,ushering in a new era in the treatment of pe-ripheral nerve injury.

BACKGROUND:Aberrant expression of some transcription factors involved in the Heghog signaling pathway occurs in patients with polydactyly,and these transcription factors regulate the expression of massive target genes,thereby affecting cellular function.OBJECTIVE:To analyze the transcriptome characterization of patients with polydactyly by single-cell transcriptome analysis.METHODS:The single-cell transcriptome data of mesenchymal and epithelial cells of four patients with polydactyly were downloaded from the GEO public database.Fibroblasts and keratinocytes were categorized into cell subsets,and the transcription factors within each subset were examined.A regulatory network of these transcription factors and their target genes was developed,and the functions of these regulatory factors were analyzed.RESULTS AND CONCLUSION:The transcriptional profiling data of individual cells indicated that the regulatory factors strongly linked to cell functionality in polydactyly include the transcription factors HOXD13,MSX2,LHX2,EMX2,LEF1,CREB3L2,and LHX2 of the HOX family and GLI2 transcription factors.In fibroblasts,HOXD13,MSX2,and LHX2 are involved in polydactyly,whereas HES2 and GLIS1 are crucial for the formation and development of keratinocytes.To conclude,the elevated expression of transcription factors including HOXD13,MSX2,and LHX2 is potentially closely associated with the development of polydactyly.Potential therapeutic strategies that may be offered to correct or prevent polydactyly by targeting specific transcription factors or modulating their activity.

Objective:Peripheral nerve injury(PNI)is the most common clinical condition.In this study,the current hotspot and future trends of the therapeutic role of microRNA(miRNA)in posterior axon regeneration were evaluated,through bibliometric analysis of peripheral nerves,to provide a reliable reference for later related studies.Methods:Published data from 2009 to 2024 were collected from the Web of Science database by key words TS=mi-croRNA OR TS=miRNA AND TS=peripheral nerve injury OR TS=microRNA OR TS=miRNA AND TS=axonal re-generation screening.The visualization analysis software CiteSpace was used to evaluate the author,country,keywords,outbreak words,institutions,etc.Results:A total of 301 documents were retrieved from the Web of Science database,and 288 documents finally fit the study post eliminating and screening.The 288 articles used in this study have a total of 1513 authors from 34 countries,and 1513 authors from 358 institutions published in 148 journals,citing 13941 articles from 2038 journals.In addition,this study lists the top 5 authors of the annual publication,national distribution,5 arti-cles with the most cited times and the top ten institutions of articles.Moreover,among them,China is the country with the largest number of articles,but there is still a significant gap in the quality of articles when compared with European and American regions.The analysis of institutions found that domestic institutions account for the largest proportion of the research in this field,with close mutual cooperation,but lack of cooperation with foreign institutions.The most cited article in research is"Let-7 microRNAs Regenerate Peripheral Nerve Regeneration by Targeting Nerve Growth Factor"which was cited 34 times.The institution with the largest publication volume is 31 articles from Nantong University.Conclusion:Although the research on therapeutic role of tiny RNA in axon regeneration after peripheral nerve injury started late,the number of papers is relatively small,but it has great potential.As the most popular topic today,tiny RNA has been inuetigated regarding its mechanistic action in the fields of nerve inflammation,nerve cell repair and re-generation,neuroprotection and functional recovery.With the investment in scientific research in this field,more high-quality articles will be published in excellent journals at home and abroad,ushering in a new era in the treatment of pe-ripheral nerve injury.

BACKGROUND:Successful regeneration of the peripheral nervous system relies on the damaged neuronal axons and non-neuronal cells,including Schwann cells and immune cells.The cellular processes that promote or limit axonal regeneration will be instructive in improving prognosis after peripheral nerve injury.OBJECTIVE:To review the mechanisms and recent therapeutic advances in peripheral nerve axonal degeneration and regeneration during Wallerian degeneration.METHODS:Utilizing computer-based searches,relevant articles published between 2004 and 2024 were retrieved from the Web of Science Core Collection,WanFang Database,and CNKI Database.The search terms included"peripheral nerve injury,axon injury,axon degeneration,Wallerian degeneration,cells,mechanism"in English and Chinese.Finally,108 articles were included for review.RESULTS AND CONCLUSION:Wallerian degeneration is a dynamic cellular process that is tightly regulated at the cellular and molecular levels.By unravelling the major roles and cellular molecular biological basis of Wallerian degeneration,it provides a useful framework for understanding Wallerian and Wallerian-like degeneration and lays the foundation for developing new therapeutic strategies to treat peripheral nerve injuries and neurodegenerative diseases.

Objective:In this study,bibliometric methods were used to analyze the research hotspots and future de-velopment trends of mesenchymal stem cells in peripheral nerve injury and regeneration.Method:Data on relevant arti-cles published between 2013.01.01 and 2024.06.01 were collected by filtering using keywords in the Web of Science database.These articles were analyzed by utilizing VOS viewer,and Cite Space bibliometric software.Results:In this study,we searched the Web of Science database extensively and retrieved a total of 386 relevant articles.We analysed the annual number of publications,country distribution,top 10 authors,top 10 most cited articles,and top 10 pub-lished institutions to gain a more comprehensive understanding of the current status and trends of peripheral nerve injury treatment and regeneration research in the last decade.Conclusion:The mesenchymal stem cell therapy for peripheral nerve injury provides new possibilities for the treatment of peripheral nerve injury,and has become an innovation in the treatment method,which is helpful to provide data and theoretical support for the field of peripheral nerve regeneration therapy,promote the development of related research,promote the development of mesenchymal stem cell therapy in clinical application,and is expected to provide patients with more effective treatment options,thereby improving their quality of life and rehabilitation process.

Objective:In this study,bibliometric methods were used to analyze the research hotspots and future de-velopment trends of mesenchymal stem cells in peripheral nerve injury and regeneration.Method:Data on relevant arti-cles published between 2013.01.01 and 2024.06.01 were collected by filtering using keywords in the Web of Science database.These articles were analyzed by utilizing VOS viewer,and Cite Space bibliometric software.Results:In this study,we searched the Web of Science database extensively and retrieved a total of 386 relevant articles.We analysed the annual number of publications,country distribution,top 10 authors,top 10 most cited articles,and top 10 pub-lished institutions to gain a more comprehensive understanding of the current status and trends of peripheral nerve injury treatment and regeneration research in the last decade.Conclusion:The mesenchymal stem cell therapy for peripheral nerve injury provides new possibilities for the treatment of peripheral nerve injury,and has become an innovation in the treatment method,which is helpful to provide data and theoretical support for the field of peripheral nerve regeneration therapy,promote the development of related research,promote the development of mesenchymal stem cell therapy in clinical application,and is expected to provide patients with more effective treatment options,thereby improving their quality of life and rehabilitation process.

BACKGROUND:Successful regeneration of the peripheral nervous system relies on the damaged neuronal axons and non-neuronal cells,including Schwann cells and immune cells.The cellular processes that promote or limit axonal regeneration will be instructive in improving prognosis after peripheral nerve injury.OBJECTIVE:To review the mechanisms and recent therapeutic advances in peripheral nerve axonal degeneration and regeneration during Wallerian degeneration.METHODS:Utilizing computer-based searches,relevant articles published between 2004 and 2024 were retrieved from the Web of Science Core Collection,WanFang Database,and CNKI Database.The search terms included"peripheral nerve injury,axon injury,axon degeneration,Wallerian degeneration,cells,mechanism"in English and Chinese.Finally,108 articles were included for review.RESULTS AND CONCLUSION:Wallerian degeneration is a dynamic cellular process that is tightly regulated at the cellular and molecular levels.By unravelling the major roles and cellular molecular biological basis of Wallerian degeneration,it provides a useful framework for understanding Wallerian and Wallerian-like degeneration and lays the foundation for developing new therapeutic strategies to treat peripheral nerve injuries and neurodegenerative diseases.

BACKGROUND:Aberrant expression of some transcription factors involved in the Heghog signaling pathway occurs in patients with polydactyly,and these transcription factors regulate the expression of massive target genes,thereby affecting cellular function.OBJECTIVE:To analyze the transcriptome characterization of patients with polydactyly by single-cell transcriptome analysis.METHODS:The single-cell transcriptome data of mesenchymal and epithelial cells of four patients with polydactyly were downloaded from the GEO public database.Fibroblasts and keratinocytes were categorized into cell subsets,and the transcription factors within each subset were examined.A regulatory network of these transcription factors and their target genes was developed,and the functions of these regulatory factors were analyzed.RESULTS AND CONCLUSION:The transcriptional profiling data of individual cells indicated that the regulatory factors strongly linked to cell functionality in polydactyly include the transcription factors HOXD13,MSX2,LHX2,EMX2,LEF1,CREB3L2,and LHX2 of the HOX family and GLI2 transcription factors.In fibroblasts,HOXD13,MSX2,and LHX2 are involved in polydactyly,whereas HES2 and GLIS1 are crucial for the formation and development of keratinocytes.To conclude,the elevated expression of transcription factors including HOXD13,MSX2,and LHX2 is potentially closely associated with the development of polydactyly.Potential therapeutic strategies that may be offered to correct or prevent polydactyly by targeting specific transcription factors or modulating their activity.