Objective To construct the competency of traditional Chinese medicine(TCM)rehabilitation based on World Health Or-ganization rehabilitation competency framework(RCF). Methods The competency requirements of TCM rehabilitation in higher TCM institutions were analyzed using RCF the-ory and method,through literature analysis and expert surveys, Results A comprehensive analysis was conducted to extract the practice,professionalism,learning and development,and research competency of TCM rehabilitation professionals,considering the characteristics of the educational envi-ronment in TCM institutions,the training orientation of TCM rehabilitation programs,core knowledge and ethi-cal requirements. Conclusion The adoption of the RCF to explore the competency of TCM rehabilitation professionals helps enhance the professionalism and applicability of the TCM rehabilitation field,providing ideas and methods for cultivating high-quality TCM rehabilitation professionals.

This paper expounds the medical ethic qualities of Qiu Fazu's putting the patients'interests above all else,including the attitude of treating patients with excellent medical skills and benevolent love,the working style of being highly responsible and careful,the never-ending academic spirit of being rigorous and realistic,the medical concept of strict enthusiasm and sincere care,and the moral quality of indifference to fame and wealth and no partiality.It is indicated that family education environment,excellent traditional Chinese culture,moral models leading are the formation logic of Qiu Fazu's medical ethics.Qiu Fazu's views on medical ethics are of great value to the education of medical ethics in the new era.To improve the level and effectiveness of medical ethics education,it is necessary to strengthen the excellent traditional Chinese culture education,strengthen international exchanges and cooperation,lead the advanced moral examples,and cultivate social practice ability.

Objective To explore the improvement value of Peiyuan Tongnao capsule on nerve function in patients.Methods Retrospective cohort analysis of patients with ischemic stroke was performed.Control group was treated for 12 weeks by oral aspirin enteric-coated tablets(100 mg,qd each time),intravenous infusion of low molecular dextran 500 mL(qd),oral atorvastatin calcium 20 mg(qd);the treatment group was treated with Peiyuan Tongnao capsule 1.8 g(tid)for 12 weeks on the basis of the control group.After 12 weeks of treatment,clinical efficacy,neurological function[National Institutes of Health Neurological impairment(NIHSS)score],traditional Chinese medicine(TCM)syndrome score,laboratory indexes[calcitonin gene-related peptide(CGRP),plasma D-dimer(D-D),neutrophil to lymphocyte ratio(NLR)],and hemorheological indexes[whole blood viscosity,platelets aggregation rate,fibrinogen(FIB),blood sedimentation rate(ESR)]were compared between the two groups,and incidence of adverse drug reactions during treatment.Results After 12 weeks of treatment,the total effective rate of treatment group and control group was 77.50％(31 cases/40 cases)and 55.00％(22 cases/40 cases),respectively,and the difference was statistically significant(P＜0.05).After 12 weeks of treatment,the NIHSS scores of treatment group and control group were(6.09±1.03)and(10.23±2.03)points;the TCM syndrome scores were(12.57±2.82)and(15.78±3.12)points;CGRP levels were(35.47±6.24)and(30.12±6.11)pg·mL-1;D-D levels were(0.31±0.09)and(0.56±0.11)mg·mL-1;NLR levels were 4.76±1.23 and 3.06±0.91;whole blood viscosity were(3.11±0.62)and(3.74±0.84)mPa·s-1;platelet aggregation rate were(48.21±5.11)％and(54.69±6.31)％;FIB level were(2.41±0.32)and(3.11±0.49)g·L-1;ESR were(18.11±2.06)and(23.45±2.15)mm·h-1,respectively.The above indexes in treatment group were significantly different from those in control group(all P＜0.05).There were 6 cases of adverse drug reactions in the treatment group,and the incidence of adverse drug reactions was 15.00％,while 8 cases in the control group,and the total incidence of adverse drug reactions was 20.00％,the difference was not statistically significant(P＞0.05).Conclusion Conventional treatment combined with Peiyuan Tongnao capsule has better efficacy than conventional treatment for ischemic stroke,which can improve nerve function and hemorheology and improve clinical efficacy.

Objective To evaluate the efficacy and safety of Shenbao tablet in the treatment of kidney-yang deficiency syndrome.Methods Patients with kidney-yang deficiency syndrome,will were treated with Shenbao Tablets orally,3 tablets once,3 times a day,and the course of treatment was 56 days or 84 days,depending on the condition.By comparing the clinical efficacy,the changes of symptom scores and syndrome scores before and after treatment,and symptoms remission time,the effectiveness of Shenbao tablet in the treatment of kidney-yang deficiency syndrome was evaluated.The safety was evaluated by adverse reactions.Results There were 339 patients in the 56-day group and 345 patients in the 84-day group.After treatment,the clinical effective rates of the 56-day group and the 84-day group were 91.74％and 97.97％,respectively,and the difference was statistically significant(P＜0.05).In the 56-day group and the 84-day group,the excellent rate were 58.41％and 59.13％,clinical control rates were 18.58％and 27.54％,and the progress rates were 14.75％and 11.30％,respectively.After treatment,the symptom scores of kidney-yang deficiency syndrome were significantly reduced respectively within both groups(P＜0.05).In the 56-day group and the 84-day group,the nocturia scores of were 0.89±1.27 and 0.60±1.03,the soreness of waist scores were 1.31±1.19 and 0.72±1.00,the morning diarrhea scores were 0.28±0.74 and 0.19±0.61,the anaphrodisia scores were 0.65±1.13 and 0.53±0.98,the low spirits scores were 0.29±0.81 and 0.08±0.40,the cold limbs score were 1.09±1.20 and 0.55±0.93,the edema scores were 0.14±0.55 and 0.05±0.30,the bright pale complexion scores were 0.20±0.59 and 0.24±0.65,respectively.There were significant differences in the reduction of each symptom score between the two groups(P＜0.05);the 56-day group had a more significant decrease in the score of cold limbs than the 84-day group.The remaining symptom scores decreased more significantly in the 84-day group.After treatment,the syndrome scores of kidney-yang deficiency syndrome in the two groups were significantly lower than those before treatment(all P＜0.05);the change rates of score in the 56-day group and the 84-day group were(-72.33±24.57)％and(-78.77±19.53)％,respectively,and the difference was statistically significant(P＜0.05).The self-reported time to first symptom relief was(14.85±7.18)days in the 56-day group and(14.10±7.78)days in the 84-day group,with no significant difference(P＞0.05).The incidence of adverse reactions of Shenbao tablets was 5.37％,mainly reflected in hepatobiliary system diseases,gastrointestinal system diseases and various examination abnormalities.Conclusions After taking Shenbao tablets for 2 to 3 months,the clinical symptoms of kidney-yang deficiency were better improved,and the improvement was more significant after 3 months of treatment.The security of Shenbao Tablets was good.

Objective To investigate the effect of quercetin on HCE-2 injury of human corneal epithelial cells induced by high osmotic pressure and its mechanism.Methods HCE-2 cells were randomly divided into control group(normal osmotic pressure),model group(high osmotic pressure),experimental-L group(high osmotic pressure+31.25 pg·mL-1 quercetin),experimental-M group(high osmotic pressure+62.50 μg·mL-1 quercetin),experimental-H group(high osmotic pressure+125.00 μg·mL-1 quercetin),erastin group(high osmotic pressure+125.00 μg·mL-1 quercetin+30.00 μmol·L-1 iron death inducer erastin).Cell survival rate was detected by cell counting kit 8;reactive oxygen species(ROS)levels was detected by C11-BODIPY 581/591 probe staining;glutathione(GSH)and malondialdehyde(MDA)levels were determined by kit method;the expression levels of glutathione peroxidase 4(GPX4),dihydrolactate dehydrogenase(DHODH)and ferroptosis suppressor protein 1(FSP1)were detected by real-time quantitative polymerase chain reaction and Western blot.Results The cell survival rates of control group,model group,experimental-H group and erastin group were(100.00±3.97)％,(50.05±5.83)％,(86.35±7.35)％and(58.32±4.66)％,respectively;ROS levels were 1.00±0.09,2.45±0.16,1.19±0.05 and 2.09±0.30,respectively;GPX4 protein levels were 1.09±0.11,0.34±0.03,0.91±0.12 and 0.30±0.04,respectively;FSP1 protein levels were 0.92±0.06,0.25±0.03,0.89±0.07 and 0.39±0.07,respectively;DHODH protein levels were 0.89±0.11,0.31±0.04,0.86±0.11,0.41±0.04,respectively.Compared with model group,the above indexes in control group were statistically significant(all P＜0.05);the differences between experimental-H group and model group were statistically significant(all P＜0.05);the above indexes in erastin group were significantly different from those in experimental-H group(all P＜0.05).Conclusion Quercetin can ameliorate HCE-2 cell damage induced by high osmotic pressure by inhibiting iron death pathway.

Objective Vascular dementia(VD)is a clinical syndrome caused by various cerebrovascular diseases,including ischemic,hemorrhagic,and acute and chronic hypoxic cerebrovascular diseases,leading to impaired brain function and affecting patients'cognitive ability,daily life,and work abilities.Vascular dementia is a preventable and reversible form of dementia,second only to Alzheimer's disease as the second common cause of dementia.At present,the relevant pathogenesis of vascular dementia is not clear,and there is no clear treatment method.However,its pathogenesis may be related to neuroinflammation,oxidative stress,neuronal damage and white matter lesions.Its main risk factors include genetic factors,hypercholesterolemia,diabetes,hypertension,etc.Neuroinflammatory response plays a major role in the process of secondary brain injury caused by cerebral ischemia,and inflammatory factors lead to an inflammatory cascade reaction that exacerbates damage to the nervous system.Inhibiting the inflammatory pathway and reducing the expression of inflammatory factors can improve the symptoms of vascular dementia patients and animal models,indicating that neuroinflammation may play an important role in the pathogenesis of vascular dementia.This article explores the effects of Buyang Huanwu Decoction on inflammatory factors from the perspective of summarizing relevant literature in recent years.It mainly reviews the pharmacological effects of Buyang Huanwu Decoction on treating vascular dementia,the relationship between inflammatory factor levels and vascular dementia,and the prevention and treatment of vascular dementia by regulating inflammatory factor levels.

Sulfur dioxide(SO2)is involved in regulating various physiological processes of blood vessels,such as maintaining normal vascular structure,regulating vascular tension,controlling blood pressure,inhibiting vascular cell proliferation,regulating apoptosis and autophagy.In pathophysiological conditions such as hypertension,pulmonary hypertension and atherosclerosis,SO2 plays a protective role in pathological changes through different molecular mechanisms.In this paper,we will review the endogenous SO2 production and metabolism,vascular biological effects and its regulation on blood vessels.

Hydrogen sulfide(H2S)is an emerging endogenous neuromodulator that holds significant potential in the realm of neurological diseases.Its role encompasses reducing neuronal damage,inhibiting excessive activation of striatal astrocytes,and regulating cerebrovascular function,among other physiopathological pathways.Conversely,microRNAs(miRNAs)are widely recognized as pivotal regulators of neurological diseases.They primarily target the 3'untranslated region of the target gene mRNA,impeding mRNA translation and hindering its expression to impart neuroprotective effects.Recent findings have underscored the crucial involvement of H2S and miRNAs in the pathogenesis of various neurological disorders such as Parkinson's disease,stroke,and spinal cord diseases,thereby garnering significant attention.This paper comprehensively summarizes the protective effects arising from the interplay between H2S and miRNAs in neurological diseases,while also delving into the potential therapeutic efficacy they hold for such conditions.

[摘 要] 宫颈癌是一种发病率和转移率均较高的妇科癌症。尽管手术、放射、化学治疗在宫颈癌治疗中取得了显著的临床效果，但仍存在一定的局限性，因此亟需新的治疗药物。近年研究发现，Wnt/β-catenin 信号通路在宫颈癌发生发展、转移、耐药等过程中起关键性作用。Wnt/β-catenin信号途径参与宫颈癌的发生发展和远处转移及对化疗药物的耐受性；天然化合物如中药白藜芦醇和黄芩素等及合成化合物如伊曲康唑等均可通过此信号通路发挥抗宫颈癌的作用；非编码RNA和一些蛋白分子均可以通过此通路影响宫颈癌的发生发展。研究说明，Wnt/β-catenin信号途径是宫颈癌治疗的潜在靶点。本文归纳宫颈癌中Wnt/β-catenin信号途径的作用，以期为宫颈癌的临床研究与新药开发提供参考依据。

Objective To identify and validate co-expressed genes associated with myocardial ischemia/reperfusion injury(MI/RI)and necrotic apoptosis by bioinformatics analysis.Methods Gene expression profile data for MI/RI were obtained by GSE67308 and GSE19875 datasets from the Gene Expression Omnibus(GEO)database.Differential expression analysis was conducted on the GSE67308 dataset to identify differentially expressed genes(DEGs),followed by gene set enrichment analysis and biological pathway analysis.More-over,immune cell infiltration analysis was performed on the GSE67308 dataset.Necrotic apoptosis-related genes were retrieved from the Molecular Signatures Database and the Kyoto Encyclopedia of Genes and Genomes(KEGG).A protein-protein interaction(PPI)network was constructed by overlapping DEGs with these necrotic apoptosis-related genes to identify key genes.Furthermore,the expression pat-terns of these key genes across various cardiac cell types were analyzed using a single-cell sequencing analysis platform,and validation of key gene expression was performed using the GSE19875 dataset.Results A total of 1054 DEGs were identified,comprising 363 upregu-lated and 691 downregulated genes.Gene enrichment analysis revealed that DEGs were primarily associated with processes related to apoptosis,immune responses,and intracellular signaling regulation.Moreover,biological pathway analysis demonstrated that DEGs were predominantly involved in the regulation of signaling pathways such as tumor necrosis factor(TNF)and NF-κB.Immune infiltration anal-ysis indicated a high degree of immune infiltration,particularly with natural killer cells and monocytes,in MI/RI myocardial tissue.PPI network analysis identified Il1b,TNF,Birc3,and Ripk1as crucial genes in the context of necrotic apoptosis.Single-cell sequencing anal-ysis showed the elevated expression of key genes within white blood cells.In comparison to the control group,the MI/RI model group in the GSE19875 dataset exhibited significantly increased expression of Il1b,TNF,Birc3,and Ripk1(P<0.01).Conclusion MI/RI is strongly correlated with the TNF signaling pathway and the NF-κB signaling pathway,both of which play pivotal roles in regulating necrotic apop-tosis.Il1b,TNF,Birc3,and Ripk1emerge as key genes that concurrently regulate both MI/RI and necrotic apoptosis.It is plausible that IL-1b,TNF,Birc3,and Ripk1 may serve as critical regulatory factors in the context of necrotic apoptosis during MI/RI.