Objective To investigate the expression of matrix metalloproteinase 3(MMP3)in a mouse silicosis model induced by SiO2,and explore its role in pulmonary fibrosis.Methods Six male C57B/6 mice were randomly divided into control and silicosis groups(n=3).The silicosis model was established via intratracheal instillation of SiO2 suspension(0.2 g/kg);the control group were intratracheally instilled with the same amount of normal saline.Human pulmonary fibroblasts(HPF-a)and mouse lung fibroblasts(MLg)were treated with 5 ng/ml of transforming growth factor-β1(TGF-β1)to construct an ex vivo silicosis cell model.Masson trichrome and Sirius red staining were used to assess the effects of SiO2 on pulmonary tissue and extracellular matrix(ECM)deposition.Single-cell transcriptomics was performed on mouse lung tissue,with bioinformatics analyses identifying ECM-associated cellular composition changes and key genes.The expression and distribution of these key genes were analyzed by spatial transcriptomics.Western blotting was employed to detect Vimentin and MMP3 protein levels in mouse lung tissue and fibroblasts.Immunofluorescence staining was used to localize MMP3 in lung ECM and TGF-β1-treated fibroblasts and to evaluate its accumulation in the ECM.Results Masson's and Sirius red staining revealed fibrotic changes and significant ECM collagen deposition in mice of silicosis group.Single-cell and spatial transcriptomics identified fibroblast-associated alterations in ECM components,with MMP3 emerging as a key gene.MMP3 mRNA expression was significantly elevated in mouse lungs of silicosis group and was localized primarily to fibrotic lesions.Western blotting showed a significant increase in MMP3 protein levels in the lungs of silicosis group mice compared to control group(P<0.05).TGF-β1 treatment led to a time-dependent increase in MMP3 protein levels in HPF-a cells(P<0.05).Immunofluorescence revealed elevated MMP3 expression in the ECM of mouse lungs in silicosis group(P<0.05).When TGF-β1 treated MLg cells were seeded onto normal mouse lung ECM,MMP3 expression increased(P<0.05).Similarly,after decellularizing ECM seeded with MLg cells,MMP3 expression levels remained significantly elevated(P<0.01).Co-localization analysis showed enhanced Vimentin and MMP3 signals in and around silicotic nodules in mice of silicosis group(P<0.01).Conclusions In the mouse silicosis model,secretion of MMP3 from fibroblasts increased with TGF-β1 treatment,accumulating in the pulmonary ECM,exacerbating collagen deposition,and promoting fibrosis.MMP3 may serve as a potential therapeutic target for silicosis-induced pulmonary fibrosis.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

The ethical review work in hospitals has played an increasingly important role in protecting the rights and safety of subjects.However,in its practical process,there are inevitably a series of problems and difficulties,such as the need for a sound and perfect organizational structure of the ethical team,the need for detailed manage-ment system operation,and the need for standardized and innovative internal control supervision and assessment.It analyzes the practical issues of hospital ethical review and the optimisation strategies of ethical review are ex-plored based on institutional ethical perspectives to provide reference for improving the quality of ethical review in hospitals.

Objective To explore the mediating role of eating disorders in the relationship between body image satisfaction and anxiety symptoms among college students,and further examine the moderating effect of mindfulness on this mediating pathway.Methods By using convenience sampling,we conducted baseline and follow-up surveys among freshmen and sophomores at two universities in Xizang Autonomous Region from September to December 2021.Measurements included the Generalized Anxiety Disorder Scale,the body image satisfaction subscale of the Multidimensional Body-Self Relations Questionnaire,the Eating Attitudes Test,and the Mindful Attention Awareness Scale.Data analyses performed using SPSS 23.0 included Pearson correlation analysis,mediation(model 4),and moderation(model 58)effect analysis.Results A total of 2 993 students participated in both the baseline and follow-up surveys,including 1 007 males(34.3％)and 1,666 Tibetan and other ethnic minorities(56.8％),with an average age of 19.8(SD=1.3)years.Body image satisfaction significantly predicted anxiety symptoms,and this relationship was partially mediated by disordered eating.The presence of mindfulness moderated the impact of body image satisfaction on disordered eating and the influence of disordered eating on anxiety symptoms,indicating varying strengths of these relationships at different levels of mindfulness.Conclusion This study demonstrates that body image satisfaction can significantly predict anxiety symptoms,with disordered eating playing a mediating role in this relationship.The moderating effect of mindfulness further reveals that students with high levels of mindfulness are less likely to experience eating disorders influenced by body image dissatisfaction.Interventions for college students'mental health should consider including management of body image and eating behaviors,as well as mindfulness training.