Objective To establish the method of simultaneous determination of four main components of Danggui Liuhuang Decoction,including phellodendrine,palmatine,calycosin,and ferulic acid and provide reference for the quality control of Danggui Liuhuang Decoction.Methods Based on the HPLC-MS/MS analysis method,the positive ion data acquisition mode were adopted for the mass spectrometry detection and the four main components were quantified with multiple reaction monitoring mode(MRM)by ESI source.The chromatographic column was Agilent Extend-C18(5 μm,4.6 mm×250 mm),and gradient elution was performed with methanol and 0.5%formic acid in water.Results The linear range of phellodendrine was from 2-200 nmol/ml,and the linear range of palmatine,calycosin and ferulic acid was from 20-2 000 nmol/ml.The contents of the four components in the seven batches of Danggui Liuhuang Decoction were relatively stable,among which ferulic acid was mainly found in Phellodendrine and Coptidis;Phellodendrine was only detected in cortex phellodendri;the content of calycosin in Scutellaria baicalensis and Astragalus was higher;palmatine was detected in both Phellodendron and Astragalus.Conclusion The method had high sensitivity,good specificity and sample stability,which could meet the requirements of quantitative analysis of Traditional Chinese Medicine compounds,and could provide reference for further pharmacokinetics study on the content changes of traditional Chinese medicine compounds in biological samples.

Objective To prepare a novel photosensitive nanoparticle and to evaluate its physicochemical properties,and effect on the efficacy of photodynamic therapy.Methods 5,15-dibromo-10,20-diphenylporphine(DBN),tetrafluoroterephthalonitrile(TFN),and the amphiphilic polymer methoxy-polyethylene glycol-distearoylphosphatidylethanolamine(DSPE-MPEG2000,PEG)were dissolved in tetrahydrofuran(THF)by co-precipitation method to prepare novel photosensitive nanoparticles,named DBN/TFN@PEG.The physicochemical properties of DBN/TFN@PEG were characterized.Both novel and conventional nanoparticles were continuously irradiated with a 660 nm laser,and the fluorescence intensity of nanoparticles,representing reactive oxygen species(ROS)production levels,was measured using a fluorescence spectrophotometer at different irradiation times.Tumor cells were co-incubated with the nanoparticles and irradiated with a 660 nm laser.ROS levels within the tumor cells were detected using immunofluorescence,and the ratio of dead to live tumor cells was determined using PI/Calcein-AM staining.Results Prepared DBN/TFN@PEG nanoparticles with hydrated particle size of approximately 107.8 nm were uniformly distributed in the solution.Compared to conventional nanoparticles,the ROS production capacity of DBN/TFN@PEG was significantly higher(P＜0.01).Immunofluorescence results showed that the generation of ROS levels in the tumor cells of DBN/TFN@PEG group were significantly higher than in the conventional nanoparticles group under laser irradiation(P＜0.01).PI/Calcein-AM staining results indicated a significantly higher ratio of dead tumor cells in the DBN/TFN@PEG group compared to the conventional nanoparticle group(P＜0.01).Conclusions DBN/TFN@PEG has stable physicochemical properties and uniform distribution in the solution.As effective photosensitizers,DBN/TFN@PEG can exhibit stronger ability to induce ROS generation in tumor cells,and may enhance the efficacy of photodynamic therapy in cancer.

Objective:To investigate the clinicopathological features, immunophenotype, diagnosis and differential diagnosis of SRF-rearranged cellular perivascular myoid tumor.Methods:Two cases of SRF-rearranged cellular perivascular myoid tumor diagnosed in the Department of Pathology, Fudan University Shanghai Cancer Center from October 2021 to March 2022 were collected. Immunohistochemical staining, fluorescence in-situ hybridization (FISH) and next-generation sequencing (NGS) were performed, and the literature was reviewed.Results:Case 1, a 3-month-old boy presented with a painless tumor of the scalp, measuring about 2 cm in diameter. Case 2, a 3-year-old girl complained with a painless tumor of the knee, measuring approximately 1.5 cm in diameter. Microscopically, the tumor had a clear boundary and showed multinodular growth. The tumor was mainly composed of spindle cells arranged in long intersecting fascicles associated with thin, slit-like or branching ectatic vessels, focally forming hemangiopericytoma-like appearance. The tumor cells were abundant, but there was no obvious atypia. Mitotic figures (3-4/10 HPF) were noted. H-caldesmon and SMA were positive in both cases. Case 1 showed diffuse and strong positivity for Desmin, and focally for CKpan. Ki-67 proliferation index was 20% and 30%, respectively. FISH displayed NCOA2 gene translocation in case 1 and the RELA gene translocation in case 2. NGS detected the SRF-NCOA2 gene fusion in case 1 and the SRF-RELA gene fusion in case 2. Both patients underwent local excisions. During the follow-up of 5-14 months, case 1 had no local recurrence, while case 2 developed local recurrence 1 year post operatively.Conclusions:SRF-rearranged cellular perivascular myoid tumor is a novel variant of perivascular cell tumor, which tends to occur in children and adolescents. The tumor forms a broad morphologic spectrum ranging from a pericytic pattern to a myoid pattern, and include hybrid tumors with a mixture of pericytic and myoid patterns. Due to its diffuse hypercellularity and increased mitotic figures and smooth muscle-like immunophenotype, the tumor is easy to be misdiagnosed as myogenic sarcomas. The tumor usually pursues a benign clinical course and rare cases may locally recur.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.

Objective Under the background of building age-friendly hospitals,it aims to explore the satisfaction and influencing factors of elderly patients in Shanghai City,which has the highest degree of aging in China,in order to provide reference for improving the age-friendly hospitals.Methods According to the framework of building age-friendly hospitals,a survey questionnaire is designed around friendly services and friendly environment,and the Likert 5-level rating is used.Select one medical institution in the central urban area and one in the suburbs of Shanghai City,both of which are grade-A tertiary age-friendly hospitals,and outpatient patients were randomly selected to participate in the questionnaire survey.A total of 2 418 elderly patients(≥60 years old),were ultimately surveyed.Results Elderly patients'overall satisfaction with medical care,with an average score of 4.77,is very satisfactory;the ratings of friendly service and friendly environment are very good,but relatively speaking,the friendly service is better and the friendly environment is weaker.Among elderly patients,younger(60～74 years old),male,and those living in other places have higher satisfaction.Friendly service and friendly environment are both influencing factors of satisfaction,with friendly service having a greater impact weight.Conclusion Good friendly service should be maintained,and weak friendly environment should be strengthened.Continuously improve age-friendly hospitals and enhance the medical experience of elderly patients.