This study aimed at defining the anti-allergic effects of Acanthopanax senticosus Root Extract (EUE) and Perilla frutescens Seed Extract (OSE) used alone or in combination, with the following results. (1) In the histamine release test, histamine release was significantly lower in both EUE and OSE groups than in the control group (p<0.01). EUE combined with OSE at 5:1 also showed a stronger inhibitory effect against histamine release than when used separately. (2) In the PCA test, oral administration of EUE for 14 days either alone or in combination with OSE resulted in dose-dependent inhibition of pigment leak as compared with the control group (p<0.01). In addition, although the beneficial effect of EUE combined with OSE was no greater than that obtained by EUE alone, it was definitely greater than by DSCG (Intarl inhalant solution). (3) An investigation of the effect of EUE against pigment leak induced by histamine and serotonin showed a dose-dependent inhibitory tendency (not statistically significant). In conclusion, EUE and OSE might have anti-allergic effects.