Objective To screening myocardial Inflammatory gene and its function in myocardial injury after single bout of exhausted exercises.Methods Fifry male Sprague-Dawley rats were randomly divided into sedentary control groups and single bout of exhausted exercise groups.Rats were killed at 0,6,12 and 24 h after single bout of exhausted exercises,and the gene chip technology was used to screening myocardial inflammatory gene.Results After single bout of exhausted exercise,myocardial inflammatory gene mainly involved with Cc117, PF4, CCL2, CCL21b, CCL7, Cc112_predicted, I16r, I11r2, Ifngr, Ccr12 predicted,ICAM-1 and Adamts1,with a trend of inrease to decrease.The gene expression of Ce117, PF4, Cc112_predicted, I116r, I11r2,Ifngr,Ccr12_predicted and Adamtsl shgnificant significant apgrade at oh after exercise,and the gene expression of CCL2,CCL21b,CCL7 and ICAM-1 showed significant upgrade at 6h after exercise.Conclusions The significantly increase expression of the chemotaetic factors and their receptors,mediate inflammdtory cells to the ischemia myocardium might be the patho-mechanism of exercise-induced yocardial micro damage after single bout of exhausted exercise.