Natural Product Sciences  2015;21(4):261-267

doi:10.20307/nps.2015.21.4.261

Deuteromethylactin B from a Freshwater-derived Streptomyces sp..

Anam F SHAIKH 1 ; Maryam ELFEKI ; Samantha LANDOLFA ; Urszula TANOUYE ; Stefan J GREEN ; Brian T MURPHY

Affiliations

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Keywords

Actinomycete; Freshwater; Streptomyces; Octalactin

Country

Republic of Korea

Language

English

Abstract

Compared to their terrestrial and marine counterparts, little is known about the capacity of freshwater-derived actinomycete bacteria to produce novel secondary metabolites. In the current study, we highlight the disparities that exist between cultivation-independent and -dependent analyses of actinomycete communities from four locations in Lake Michigan sediment. Furthermore, through phylogenetic analysis of strains isolated from these locations, we identified a Streptomyces sp., strain B025, as being distinct from other Streptomyces spp. isolated from sediment. Upon fermentation this strain produced a rare class of eight-membered lactone secondary metabolites, which have been for their antitumor properties. We used spectroscopic and chemical derivitization techniques to characterize octalactin B (1) in addition to its corresponding novel, unnatural degradation product (2).