Brain Tumor Research and Treatment  2014;2(1):22-28

doi:10.14791/btrt.2014.2.1.22

Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion.

Jian PEI 1 ; Kyung Sub MOON ; Sango PAN ; Kyung Hwa LEE ; Hyang Hwa RYU ; Tae Young JUNG ; In Young KIM ; Woo Yeol JANG ; Chae Hun JUNG ; Shin JUNG

Affiliations

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Keywords

Cathepsin D; Gliomas; Invasion; Motility; Protein; Proteomics

Country

Republic of Korea

Language

English

Abstract

BACKGROUND: To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments. METHODS: High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed. RESULTS: Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A. CONCLUSION: Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.