Chinese Journal of Veterinary Science 2003;23(1):1-3
Polymerase Chain Reaction Detection for Porcine Endogenous Retrovirus in 4 Pig Cell Lines
Maomin LU ; Hong JIN ; Ruichun DENG ; Jianguo WANG ; Shu YANG ; Jingfeng XIONG ; Zhuang DING ; Jingang ZHANG
Keywords
porcine endogenous retrovirus; polymerase chain reaction; detection; pig cell lines
Country
China
Language
Chinese
Abstract
Porcine endogenous retroviruses (PERV) can infect human cell in vitro, which raised widely concerns re-garding the transmission of PERV to xenograft recipients. It's essential to establish a method for detection of PERV.3 pairs of primers were synthesized according to the sequence of gag, pol and env gene of PERV. Polymerase chainreaction (PCR) and reverse transcription PCR (RT-PCR) assays were performed for detection of PERV provirusDNA and PERV specific mRNA. The results showed that provirus DNA and mRNA of PERV existed and expressedin all 4 tested cell lines. The sizes of amplified fragments are identical with the predicted. These methods may be suit-able for monitoring PERV in other cells or tissue.
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