Chinese Herbal Medicines 2023;15(4):533-541

doi:10.1016/j.chmed.2023.01.006

Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro.

Kehinde Oluseun SODEINDE 1 ; Akinwunmi Oluwaseun ADEOYE 2 ; Adedeji ADESIPO 1 ; Adebayo A ADENIYI 1 ; John Adeolu FALODE 2 ; Tajudeen Olabisi OBAFEMI 3 ; Samuel Olalekan OLUSANYA 1 ; Linette TWIGGE 4 ; Jeanet CONRADIE 4 ; Timothy O MOSAKU 5

Affiliations

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Keywords

Anchomanes difformis (Blume) Engl.; ergosterol; mitochondrial membrane; modulation; transition pore; xylopic acid; β-stigmasterol

Country

China

Language

English

Abstract

OBJECTIVE:Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol (with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore (mPTP).

METHODS:The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.

RESULTS:1H and 13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.

CONCLUSION:Reduction in the activity of calcium ATPase and the expression of Caspase-3 and -9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.