Journal of Veterinary Science  2009;10(4):299-304

doi:10.4142/jvs.2009.10.4.299

Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium.

So Yeong LEE 1 ; Kyong Ah YOON ; Soo Hwa JANG ; Erdene Ochir GANBOLD ; Dembereldorj UURIINTUYA ; Sang Mo SHIN ; Pan Dong RYU ; Sang Woo JOO

Affiliations

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Keywords

cancer cell screening; cell monitoring; infrared spectroscopy; lung cancers; phosphorylation

Country

Republic of Korea

Language

English

Abstract

The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching nus(PO3(2-)) band intensity at ~970 cm-1 and the phosphodiester symmetric stretching nus(PO2-) band intensity at ~1,085 cm-1 in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other vibrational bands compared to normal cells. This finding suggests that more extensive phosphorylation occur in cancer cells. These results demonstrate that lung cancer cells may be prescreened using infrared spectroscopy tools.