Malaysian Journal of Microbiology  2018;14(6):579-584

Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate

Nashriq Mohammad Jailani 1 ; Asmahani Azira Abdu Sani 2 ; Roman Fischer 3 ; Honglei Huang 3 ; Wan Mohd Aizat 1 ; Sheila Nathan 1 ; Holger B. Kramer 4 ; Benedikt M. Kessler 3 ; Mukram Mohamed Mackeen 1

Affiliations

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Keywords

GASP; in-solution digestion; proteomic analysis; Saccharomyces cerevisiae

Country

Malaysia

Language

English

Abstract

Abstracts:Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GASP afforded the highest number of overall protein identifications and peptide spectrum matches without systematic bias towards peptide or protein size.