Chinese Journal of Zoonoses 2010;(1):62-64

doi:10.3969/j.issn.1002-2694.2010.01.015

Sequence analyses of the Cps2J gene in 9 strains of Streptococcus suis serotype2 isolated in Zhejiang province

Pingping YAO ; Fusu WANG ; Hanping ZHU ; Lingling MEI ; Juyian YE ; Jin LUO ; Zheng ZHANG ; Chenhui YAO

Keywords

Streptococcus suis serotype 2; Cps2Jgene; sequence analyses

Country

China

Language

Chinese

Abstract

To explore the molecular characteristics of Streptococcus suis serotype 2(ss2) isolated in Zhejiang province by deciding the variation loci and its variation frequency of Cps2J gene.The total DNA of 9 strains of ss2 isolated in Zhejiang province were extracted and amplifed by PCR. Then,the Cps2J fragments were cloned into plasmid carrier and completely sequenced after purification.Finally,the sequence results of all 9 ss2 isolates were compared with those obtained by other studies around the world.It was found that the open reading fragments of Cps2J in 9 SS2 isolates encoding 333 amino acids were 999 bp in length.Comparisons of this region among ss2 isolates revealed a similarity of between 98.8% and 99.9%, while the homology to ss1 strains varied between 56.8% and 57.0%.Our study shows the sequences of complete Cps2J segment are fairly stable and all these 9 ss2 strains of different sources possibly have the same evolutionary origin.