Chinese Journal of Veterinary Science 1999;19(3):207-211

Proviral Genomic Variation of EIAV Envelop gp 90 Gene Observed in Experimentally Infected Horse

Quankai WANG ; Hai PANG ; Xiangang KONG ; Hua XUAN ; Jingang ZHANG ; Jingliang LU ; Enge FEI

Keywords

equine infectious anemia virus; gp90 gene; provirus; variation; nucleotide sequence

Country

China

Language

Chinese

Abstract

The present experiment was designed to observe the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in infected horse. One horse was infected experimentally with P337-V70 strain and showed no clinical signs after being infected at twice with the same virus strain. Seventeen proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were obtained from the buffy coat and liver of the horse through PCR amplification and cloning. Comparative analysis of the sequences revealed that some sequences contained the nucleotide insertions in the PND region. The insertions might be generated by direct repeat and strand displacement of sequence segment in their PND gene, showing different lenghts.