Mongolian Pharmacy and Pharmacology 2016;9(2):69-74

A Basic Knowledge for Processing HPLC Data Analysis

А.Баянмөнх ; М.Уранчимэг ; Л.Лхагва ; Л.Хүрэлбаатар ; Монос групп ; Эм судлалын хүрээлэн ; Эрүүл мэндийн яам ; Эм эмнэлэгийн хэрэгслийн хэлтэс

Keywords

peak, wavelength, baseline,

Country

Mongolia

Language

Mongolian

Abstract

"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."