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Quantitative analysis of cervical vertebral maturation in Chinese adolescents based on three-dimensional morphology of cervi-cal vertebrae

Yue WU ; Wen TANG ; Yuyanran ZHANG ; Weiyu YUAN ; Yifei PAN ; Xinyu CHEN ; Haiyang XU ; Yunfan LYU ; Iman IZADIKHAH ; Dan CAO ; Lizhe XIE ; Bin YAN

STOMATOLOGY.2024;44(5):321-328. doi:10.13591/j.cnki.kqyx.2024.05.001

Objective To investigate associations between three-dimensional(3D)morphology of cervical vertebrae and skeletal mat-uration by cone-beam computed tomography(CBCT)and establish corresponding regression models for quantitatively evaluating cervical vertebral maturation(CVM).Methods The analyzed sample consisted of 358 CBCT images(175 male,183 female),of which 277 images were randomly selected as the model development group and 81 as the performance test group.Twenty-one 3D morphological pa-rameters were defined and measured,incorporating all parts of the cervical vertebrae,including the cervical vertebral bodies,transverse processes,spinous processes,pedicles,lamina,and articular processes.The cervical vertebral maturation index(CVMI)was determined by experienced orthodontists as reference standard.Spearman's rank correlation coefficient and multivariable stepwise regression analysis were used to identify the associations and build regression models.The performance test group was employed to ex-amine each model's reliability.Paired-samples Wilcoxon signed-rank test compared the CVMI of the model prediction with the reference standard.Results Three-dimensional morphological changes in various parts of the cervical vertebrae correlated with CVMI(P＜0.05).Six 3D morphometric parameters were each recognized for male and female models,three of which were identical.The adjusted R2 was 0.899 for males and 0.902 for females,with corresponding accuracies of 85.0%and 85.4%,respectively.These models showed no difference as compared with the reference standard(P＞0.05).Con-clusion New associations were found between 3D morphology of cer-vical vertebrae and skeletal maturation.The 3D-driven morphometric CVM assessment method and corresponding regression models exhibited good credibility and high consistency with experts.

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A regression analysis of the overcorrection for the mandibular anterior teeth in trusion with clear aligners

Yangyang HUANG ; Shiyu WANG ; Hao LIU ; Li YANG ; Penglai WANG ; Changyong YUAN

STOMATOLOGY.2024;44(5):329-333,343. doi:10.13591/j.cnki.kqyx.2024.05.002

Objective To explore an overcorrection quantization method and related influencing factors through analyzing relationships between the achieved and preset intrusion values of mandibular anterior teeth with clear aligners.Methods Twenty pa-tients receiving Invisalign were recruited.The relative intrusion values in the ClinCheck software were recorded as the preset intrusion.The achieved intrusion values were measured through the digital model superimposition.Statistical analysis was conducted to assess the differences and linear relationships between the preset and achieved intrusion values,and investigate the effect of related factors such as intrusion amounts on the intrusion efficiency.Results For the mandibular anterior teeth,the mean intrusion efficiency was 62.2%,with the highest in the central incisors and the lowest in the canines.The intrusion amounts,incisors labial inclinations,and canine at-tachment types affected the intrusion efficiency.The differences between the preset and achieved values were significant,and the linear relationship existed.The formula of the intrusion overcorrection for the mandibular anterior teeth is"Z=(W-0.110)/0.533-W".Z re-presents the overcorrection and W represents the ideal intrusion.Conclusion The preset intrusion values in the treatment protocol could not be fully achieved.Moreover,correction should be designed in cases of mandibular canine intrusion,large amountsof intru-sion,orlingually inclined incisors.Compared to the optimized attachments,the vertical rectangular attachments on the mandibular ca-nines could improve the efficiency.

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The effect of different tooth extraction patterns on Bolton index and occlusal relationship of skeletal Class Ⅱ malocclusion

Ye SONG ; Yinting REN ; Yihan ZHANG ; Jingying HAN

STOMATOLOGY.2024;44(5):334-337,355. doi:10.13591/j.cnki.kqyx.2024.05.003

Objective To investigate the effect of different premolar extraction patterns on Bolton index and occlusal relationship of skeletal Class Ⅱmalocclusion.Methods The plaster models of 62 patients with skeletal ClassⅡmalocclusion were selected and con-verted into digital models by iTeroElement scanner.Each model was simulated by the four premolar extraction pattrens,and tooth ar-rangement test was performed after tooth extraction.The Bolton index before and after tooth extraction was calculated,and the occlusal relationship after tooth arrangement was evaluated to explore its law.Results All the four premolar extraction patterns resulted in a significant decrease in the Bolton index over-all ratio in skeletal ClassⅡpatients,and compared with the extraction patterns of 4/4 and 4/5,the patterns of 5/4 and 5/5 resulted in a greater decrease in the Bolton index over-all ratio(P＜0.001).For skeletal classⅡmal-occlusion,the tooth arrangement test was conducted on the basis of establishing the neutral relationship of first molar.The average value of anterior teeth over-jet obtained by patterns of 4/4 and 4/5 was within the normal range(＜3 mm).The average value obtained by pat-terns of 5/4 and 5/5 was greater than the normal range(＞3 mm),and the difference was statistically significant(P＜0.001).Conclu-sion The premolar extraction patterns of 4/4 and 4/5 are more conducive to normalizing the Bolton index over-all ratio and establis-hing the better occlusal relationship for skeletal Class Ⅱ malocclusion.

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One case of double-stage orthodontic treatment of skeletal Class Ⅱ combined with impacted teeth

Kai GUO ; Xinying HAN ; Haojie MA ; Lijuan LIU ; Fanghong YANG

STOMATOLOGY.2024;44(5):338-343. doi:10.13591/j.cnki.kqyx.2024.05.004

Patients with skeletal ClassⅡ often show symptoms such as protrusion of maxillary anterior teeth,mandibular retraction,open lips and teeth,deep overbite,and deep overjet,which seriously affect the facial appearance.This article reports a case of an ado-lescent male patient with skeletal Class Ⅱ combined with impacted teeth treated by one-stage early correction and two-stage fixation.After treatment,the patient's skeletal Class Ⅱ symptoms improved significantly,including the improvement of mandibular retraction.The problem of open lips and teeth was solved;the facial appearance tended to be straight,and there was no obvious abnormality of the temporomandibular joint.This case shows that early correction can achieve good therapeutic effects on this kind of patients and can avoid or reduce the possibility of orthognathic surgery for these patients in adulthood.

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Study on the effect of fluoxetine against Candida albicans,alone or combined with fluconazole

Banruo SHI ; Qiaochu WU ; Haochen MIAO ; Xin WEI

STOMATOLOGY.2024;44(5):344-348,368. doi:10.13591/j.cnki.kqyx.2024.05.005

Objective To evaluate the inhibitory activity of fluoxetine alone and in combination with fluconazole on Candida albicans.Methods This study used standard strains of Candida albicans,clinical isolates of Candida albicans,and drug-resistant strains of Candida albicans for experiments to prepare the planktonic and biofilm states of Candida albicans,respectively.The effect of fluoxetine on Candida albicans was detected by XTT reduction method.Pharmacodynamic properties of fluoxetine against Candida albicans biofilm were determined by improved time-kill test.The morphological changes of fluoxetine on Candida albicans biofilm were observed by scanning electron microscopy,and the synergistic effect of fluoxetine combined with fluconazole on Candida albicans biofilms was detec-ted by the checkerboard dilution method.Results Fluoxetine had inhibitory effects on three types of Candida albicans biofilms.The minimum drug concentration that inhibited 50%activity(SMIC50)of biofilms of standard strains of Candida albicans and drug-resistant strains was 32 μg/mL;the SMIC50 of clinical strains of Candida albicans biofilm was 64 μg/mL.Time-kill test displayed that the bio-film activity of three types of Candida albicans significantly decreased compared to the control group at the fluoxetine concentration of 1×SMIC50 and 2×SMIC50.Scanning electron microscopy observation showed that compared with the control group,the number of fungal hyphae in the biofilm of Candida albicans treated with fluoxetine decreased and their morphology wrinkled.The checkerboard microdilu-tion method showed that the combination of fluoxetine and fluconazole exhibited a synergistic effect on the standard strain of Candida al-bicans bioflim,while showing no or antagonistic effects on the other two strains.Conclusion Fluoxetine alone has inhibitory effects on biofilms of different types of Candida albicans and has a synergistic inhibitory effect on biofilms of standard strains of Candida albicans combined with fluconazole.

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Effects of Versican V0/V1 gene silencing on biological behaviors of mouse dental papilla cells

Jiayan WU ; Haiyan HUANG ; Chenyu SONG ; Beizhan JIANG

STOMATOLOGY.2024;44(5):349-355. doi:10.13591/j.cnki.kqyx.2024.05.006

Objective To investigate the effect of Versican V0/V1 on biological behaviors of mouse dental papilla cells(mDPCs).Methods mDPCs were isolated from C57BL/6J mice at embryonic day 16.5(E16.5).A small interfering RNA(siRNA)constructed specifically for Versican V0/V1 was transfected into mDPCs.The silencing efficiency was verified by quantitative real-time fluorescence polymerase chain reaction(qRT-PCR)and immunofluorescence staining.The proliferation rate of mDPCs was determined using EdU assay;the migration ability of mDPCs was evaluated by scratch test and transwell assay.Alkaline phosphatase(ALP)staining and aliz-arin red staining were used to assess the mineralization capability of mDPCs.The molecules related to odontogenic differentiation and mineralization at mRNA levels were measured by qRT-PCR.Results After siRNA transfection,the mDPCs of si-Versican V0/V1 group showed weaker proliferation and migration abilities compared with si-NC group(P＜0.01).An enhanced ALP staining intensity,miner-alized nodule formation and up-regulations of the molecules related to odontogenic differentiation and mineralization at mRNA levels(P＜0.05)were observed in the mDPCs of si-Versican V0/V1 group.Conclusion Versican V0/V1 silencing inhibits the proliferation and migration of mDPCs,but enhances the abilities of odontogenic differentiation and mineralization.

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Modification of zinc-doped polydopamine coating on pure titanium surface and its corrosion resistance:A preliminary study

Yukun MEI ; Jianlan ZHANG ; Jing QIU

STOMATOLOGY.2024;44(5):356-361. doi:10.13591/j.cnki.kqyx.2024.05.007

Objective To prepare zinc-loaded polydopamine coating on the surface of pure titaniumand investigate its biocompatibility and corrosion resistance.Methods Pure titanium specimens were successively immersed in a dopamine alkaline solu-tion and a zinc sulfate solution to form a zinc-loaded polydopamine coating on the surface of the specimens.The smooth titanium surface and the polydopamine-coatedtitanium surface were used as the control group,and the zinc-containing polydopamine-coated titanium sur-face was used as the experimental group.Scanning electron microscopy(SEM)was used to observe the micro-morphology of the speci-men surface,and energy dispersive spectrometer(EDS)was used to determine the binding of zinc elements on the polydopamine coat-ing.The hydrophilicity of the specimen surface was detected by a contact angle meter.L-929 fibroblasts were cultured in vitro and the biocompatibility of the modified titanium surface was evaluated by CCK-8 assay.An electrochemical potentiostat was used to obtain the open-circuit potential and potentiodynamic polarization curves of specimens of each group in artificial saliva to analyze the effect of zinc-doped polydopamine coating on the corrosion resistance of titanium surface.Results SEM and EDS analyses showed that there were differences in the surface micro-morphology among three groups,and the presence of zinccould be detected on the surface of the speci-mens in the zinc-doped polydopamine coating group.The hydrophilicity of the specimen surface was enhanced after coating modification.The results of in vitro experiment revealed that the zinc-doped polydopamine coating had good biocompatibility.The open-circuit potential and potentiodynamic polarization curves obtained from electrochemical tests and their fitting data showed that the zinc-doped polydopamine coating group had the strongest corrosion resistance,followed by the polydopamine coating group,and the smooth titani-um group had the smallest.Conclusion Zinc-doped polydopamine coatingscanbe successfully prepared on titanium surfaces us-ingchemical immersion method,and the modified titanium surfacespossess good biocompatibility and corrosion resistance.

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Effect of medical ozone injection therapy on temporomandibular joint osteoarthritis and its pain in rats

Caixia LU ; Simin ZHANG ; Nigeayi·AIHEMAITI ; Xueer LI ; Zeyuan CHEN ; Maimaitituxun·TUERDI

STOMATOLOGY.2024;44(5):362-368. doi:10.13591/j.cnki.kqyx.2024.05.008

Objective To investigate the effect of medical ozone injection therapy on temporomandibular joint(TMJ)osteoarthritis and its pain in SD rats.Methods Fifity-four rats were randomly assigned according to a random number table into three groups:con-trol group,model group,and medical ozone group,with 18 rats in each group.In the control group,only physiological saline was in-jected during modeling;in the model group,only sodium iodoacetate was injected for modeling;in the medical ozone group,after in-jecting sodium iodoacetate into the joint cavity for modeling for one week,medical ozone was then injected into the joint cavity for inter-vention at a frequency of once a week,totaling 5 times.One week(week 2 after modeling),3 weeks(week 4 after modeling),and 5 weeks(week 6 after modeling)after medical ozone injections,6 rats from each group were euthanized.Mechanical withdrawal thresh-old of rats in each group was assessed before euthanasia,and the expression levels of interleukin-1β(IL-1β)in joint fluid of rats in each group were measured after euthanasia.Gross observation and modified Mankin's scoring were performed on TMJ cartilage of rats in each group after stained with Pelletier score and Safranin O-Fast Green.Results During the same time period,compared to the control group,the model group showed a significant decrease in the mechanical pain threshold of the TMJ in rats at 1 week,3 weeks,and 5 weeks(P＜0.01).The expression levels of IL-1β in the TMJ fluid increased(P＜0.01),and the Pelletier score and modified Mankin's score of TMJ cartilage increased(P＜0.01).In comparison to the model group,the medical ozone group exhibited a significant increase in the mechanical pain threshold of the TMJ in rats after 3 weeks and 5 weeks of medical ozone injections(P＜0.01).The expression levels of IL-1β in the TMJ fluid decreased(P＜0.01),and the Pelletier score and modified Mankin's score of TMJ cartilage decreased(P＜0.01).However,there were no statistically significant difference in the measured parameters in the TMJ cavity after 1 week of medical ozone injection(P＞0.05).Within the medical ozone group,compared to the 1-week treatment,the mechanical pain threshold of the TMJ increased(P＜0.01)and the expression levels of IL-1β in the TMJ fluid decreased at 3 weeks and 5 weeks(P＜0.01).However,there was no statistically significant difference in the Pelletier score and modified Mankin's score of TMJ cartilage(P＞0.05)between different treatment duration.Additionally,there were no statistically signif-icant differences in the mechanical pain threshold of the TMJ,expression levels of IL-1β in the TMJ fluid,Pelletier score,and modi-fied Mankin's score of TMJ cartilage between the medical ozone group at 3 weeks and 5 weeks(P＞0.05).Conclusion Medical ozone treatment for more than 3 weeks can improve temporomandibular joint osteoarthritis and its associated pain in rats.

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Investigation on the retentive force and fatigue property of different designed polyetheretherketone clasps

Yichen LUO ; Lin QIU ; Mingzhu GENG ; Wei ZHANG

STOMATOLOGY.2024;44(5):369-374. doi:10.13591/j.cnki.kqyx.2024.05.009

Objective By investigating the retention and fatigue performance of polyetheretherketone(PEEK)clasps with increased proportions of clasp arm engaging the undercut,an innovative method to improve PEEK clasps'performance was proposed and verified.Methods Three groups of PEEK clasps(n=10/group)with the terminal 1/3,2/3 and whole of their retentive arms engaging the 0.75 mm undercut were designed and fabricated respectively along with a group of cobalt-chrome clasps(n=10)with their terminal 1/3 of retentive arms engaging the 0.25 mm undercut.Retentive forces were recorded initially and every 1 500 cycles for a total of 15 000 in-sertion and removal fatigue cycles.Optical scanning and software analysis were applied to evaluate the deformation of the clasps by root mean square(RMS)after fatigue test.The inner surface morphology of clasps'arms was observed by scanning electron microscopy.Re-sults PEEK clasps with the whole of their retentive arms engaging the undercut exhibited highest mean retentive force(9.24±1.78)Namong other PEKK clasps,which was slightly lower than that of cobalt-chrome clasps(11.88±2.05)N.The retentive force of each group turned out to be reduced after fatigue cycles and cobalt-chrome clasps showed the greatest reduction(38.38%).The RMS of the PEEK clasps groups were of no statistical difference(P=0.111)and were lower than that of cobalt-chrome clasps(105.47±10.82)μm.Evidence of surface abrasion was observed on all groups of clasps especially on the section that engaged the undercut.Conclusion Increasing the proportion of PEEK clasp arm engaging the undercut effectively improved the retentive force and satisfied clinical re-quirements after fatigue cycles.PEEK clasps exhibited greater retention stability than cobalt-chrome clasps.It is feasible to improve the performance of PEEK clasps by increasing the proportion of clasp arm engaging the undercut.

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Evaluation of the efficacy of selective curettage in endodontic microsurgery

Ke WANG ; Jing YOU ; Wei LU

STOMATOLOGY.2024;44(5):375-380. doi:10.13591/j.cnki.kqyx.2024.05.010

Objective To evaluate the efficacy of selective curettage when apical lesions are adjacent or involved to important struc-tures in endodontic microsurgery(EMS).Methods A total of 264 patients who underwent EMS in the Department of Endodontics,The Affiliated Stomatological Hospital of Nanjing Medical University from January 2021 to January 2023 were selected as the research subjects.The cases of EMS were divided into selective curettage group(SC-EMS)and conventional curettage group(C-EMS).In SC-EMS group,patients with apical lesions involving or adjacent to important structures(mental foramen,mandibular neural tube,naso-palatine neural canal,maxillary sinus,nasal base and adjacent vital pulp teeth)were included,and the adjacent areas around impor-tant structures were selectively cureted.In C-EMS group,patients with apical lesions adjacent to important structures were treated with conventional apical curettage.The postoperative follow-up was 12 months,and the postoperative symptoms and imaging examinations of the patients were observed,and the efficacy was evaluated.The failure cases were examined after tooth extraction and preoperative path-ological review to analyze the causes of failure.Results The success rate of SC-EMS group is 90.2%,and the success rate of C-EMS group is 95.4%.The rate of success in C-EMS group was higher,but there was no statistical difference between the two groups(P＞0.05).Root fracture is the important causes for the failure of the two groups.There were 6 cases of short-term discomfort and 2 cases of long-term discomfort after SC-EMS.The C-EMS group didn't meet any short-term or long-term discomfort case.Conclusion Selective curettage has no significant effect on the success rate of EMS surgery,and is an effective method to protect important structures during surgery.Short-term discomfort may occur after surgery,and irreversible pulp damage of adjacent teeth occurs in a few cases.

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