Journal Detail Information

1

Cite

Share

Total Error and Measurement Uncertainty in Clinical Laboratory Medicine

Shishi ZHANG ; Wei WANG ; Haijian ZHAO ; Zhiguo WANG

Journal of Modern Laboratory Medicine.2016;31(5):153-156. doi:10.3969/j.issn.1671-7414.2016.05.046

In the clinical laboratory medicine,the measurement uncertainty (MU)is a relatively new concept.Over the years, experts of clinical laboratory medicine from all over the world made a great number of further researches and promote the development of MU,which led clinical laboratories to pay more and more attention to the meanings and functions of MU at the same time.However,because of the habitual using of the total error (TE)in clinical laboratories and similarities between concepts of MU and TE which easily resulted in confusion,a lot of laboratories still cannot completely accept MU.By explai-ning concepts of TE and MU and analyzing the pros and cons of models of TE and MU as well as their functions,the obj ec-tive of this paper is to help clinical laboratories make further comprehensions of TE and MU and understand how to properly use them in practice.

2

Cite

Share

Thromboelastographic Dynamic Monitoring and Clinical Application in Patients Experiencing Acute Ischemic Stroke and Receiving Reteplase

Hongxia XU ; Laichun SONG

Journal of Modern Laboratory Medicine.2016;31(5):137-139. doi:10.3969/j.issn.1671-7414.2016.05.040

Objective To observe the effect of reteplase on the thromboelastogram tracing of patients experiencing acute is-chemic stroke(AIS).Methods Selected 43 AIS inpatients in the Third People’s Hospital of Liaocheng from October,2013 to August,2015,who received reteplase less than 4.5 hours after the onset of syndrome of AIS.Blood samples were obtained before reteplase administration and at 0.5,1,2 and 4 hours after reteplase administration and measured by thromboelastogra-phy.Results The parameters R,K,Angle and MA of the thromboelastogram had changed after beginning administration of reteplase.The R value at 0.5 h after thrombolysis was significantly higher than that before thrombliysis,however,the R Value at 1h after thrombolysis was the most highest,and the K value.At 0.5 h after thrombolysis was the highest.At 0.5 h after thrombolysis MA and Angle values were the lowest,the differences were statistically significant (P<0.001).Conclu-sion The study suggests that the thromboelastogram is an useful tool for determining changes in the coagulation system of patients receiving reteplase.

3

Cite

Share

Relationship between Apoptosis Promoting Effector Molecule TFAR1 9 and Uterine Myoma

Ru BAI ; Qiuming QIU

Journal of Modern Laboratory Medicine.2016;31(5):94-96. doi:10.3969/j.issn.1671-7414.2016.05.025

Objective To examine serum TFAR19 activity in uterine myoma patients.Methods 45 of operation patients with uterine myoma in Beijing Huairou Maternal and Child Health Hospital were recruited.Among them,diameter of maximal myoma from 6 to 10 centineters were 29 (group A),diameter of maximal myoma more than 10 centineters were 16 (group B).In addition,25 unrelated healthy women of childbearing age from physical examination center were enrolled as controls (group C),all of the peripheral venous blood was collected after admission.Enzyme-linked immunosorbent assay (ELISA) was used to examine serum TFAR1 9 activity in uterine myoma patients.Results The activity of TFAR1 9 in group A,group B and group C were 25.32±1.93,14.41±2.28 and 34.79±4.65 respectively.The TFAR19 activity in each group was sta-tistically significant difference (F=7.93,P=0.01).Compared with group C,the TFAR19 activity in group B (q=5.606,P<0.001),group A (q=3.055,P=0.034)was statistically significant difference.The TFAR19 activity in uterine myoma patients subgroups (group A,group B)was statistically significant difference (q=3.086,P=0.033).Age between each groups was not statistically significant difference (F=2.086,P=0.132).Conclusion The decrease of TFAR19 can inhibit apoptosis.Participating the occurrence of uterine myoma,it is connected with diameter of myoma.The larger diameter of my-oma,the lower TFAR1 9 activity of examination will be.

4

Cite

Share

Change of Th2 2 Cells in Peripheral Blood of Patients with Primary Sj ogren’s Syndrome and Clinical Significance

Jing YU ; Qunying ZHU ; Yan CHEN

Journal of Modern Laboratory Medicine.2016;31(5):69-72. doi:10.3969/j.issn.1671-7414.2016.05.018

Objective To investigate the change of Th22 cells in peripheral blood of patients with Primary sjogren’s syndrome (pSS)and evaluate clinical significance.Methods 37 patients with pSS from January 2014 to November 2015 were enrolled the study as the observation group.Then 37 healthy adults receiving check-up during the same period were selected as con-trol groups in accordance with the proportion of 1∶1.Flow cytometry was performed to evaluate the levels of Th22 cells in peripheral blood,and tenzyme-linked immuno sorbent assay (ELISA)was used to measure the serum IL-22 levels.Pearson analysis was performed to investigate the relationship between Th22 level and serum IL-22 level,C3,C4,anti-SSA,anti-SSB, ANA antibody,EULAR Sjogren's syndrome disease activity index (ESSDAI)score in observation group.Then the levels of Th22 cells and serum IL-22 were compared among different labial gland pathologic stage in patients with pSS.Results The levels of Th22 cells and serum IL-22 in the observation group were (2.53±1.56)%,718.6±176.8 pg/ml respectively,and significantly higher than (1.24±0.51)%,258.9±72.4 pg/ml in control group (P<0.05).Th22 cell level was positively related with the level of serum IL-22,anti-SSA,anti-SSB and ESSDAI score,and negatively related with the content of C3 and C4 (P<0.05).There were no significant differences in the levels of Th22 cells and serum IL-22 different labial gland pathologic stage in patients with pSS (P>0.05).Conclusion The levels of Th22 cells and serum IL-22 significantly in-creased in patients with pSS,and related to other inflammatory indexes and disease activity,so they may participate in the genesis and development of pSS.

5

Cite

Share

Diagnosis Value of Detecting Plasma Dickkopf-1 Patients with Hepatocellular Carcinoma

Liping MAO ; Yimin HE ; Gang HAN ; Yueguo WANG

Journal of Modern Laboratory Medicine.2016;31(5):62-65. doi:10.3969/j.issn.1671-7414.2016.05.016

Objective To evaluate the diagnosis value of plasma Dickkopf-1(DKK1)in hepatocellular carcinoma (HCC)pa-tients.Methods Selected 48 patients with HCC,20 patients with liver cirrhosis (LC),20 patients with chronic hepatitis B (CHB),and all of them were clinically diagnosed in the Third People’s Hospital of Nantong City,chose 20 cases of health examination as healthy controls (HC),that were ruled out other chronic disease.Enzyme-linked immunosorbent assay (ELISA)and Abbott i2000 microparticle chemiluminescence immunoassay analyzer were used to determine the plasma DKK1 and alpha-fetoprotein (AFP)levels.At the same time,analysesed and compared the receiver operating characteristic (ROC)curve and correlation of the DKK1 and AFP results.Results The plasma level of DKK1 in patients with HCC was significantly higher than that in patients with chronic hepatitis B,cirrhosis and healthy controls (Z=-4.132~-5.828,P<0.001).The area under ROC curve (AUC)of plasma DKK1 in the diagnosis of HCC was 0.889 and 95% confidence in-terval was 0.831~0.947,when the Cut-off value of DKK1 was 565 ng/L,the sensitivity was 93.8% and specificity was 70% in diagnosing HCC.The areaunder ROC curve of AFP in the diagnosis of HCC was 0.759 and 95% confidence interval was 0.667~0.850.The AUC of DKK1 was significantly higher than that of AFP (Z=2.28,P=0.022).DKK1 and AFP in diagnosing HCC were no significantly correlated (r=0.148,P=0.316).21 patients with AFP under 20μg/L showed higer DKK1 above 565 ng/L in 48 patients with HCC.Conclusion Detection of DKK1 in plasma can be used as a complement of AFP in the diagnosis of HCC,Especially DKK1 early diagnostic value of AFP negative HCC patients is remarkable.

6

Cite

Share

Correlation Analysis of HCV-RNA,HCV-Ab and HCV-cAg

Ya LI ; Yun ZHANG ; Hai HUANG ; Di ZHANG ; Mingquan SU ; Xuchang GUO

Journal of Modern Laboratory Medicine.2016;31(5):120-122. doi:10.3969/j.issn.1671-7414.2016.05.034

Objective To investigate the correlation of HCV-RNA with detection indexes HCV-Ab and HCV-cAg in its clini-cal application effect among patients with hepatitis C.Methods HCV-cAg and HCV-Ab in 140 cases of HCV-RNA were detected by enzyme linked immunosorbent assay in cases of PCR,which were detected by real-time fluorescence quantitative PCR.Results 127 cases in 140 cases of HCV-RNA positive serum were HCV-cAg positive,in line with the rate of 90.71%,and the cases of 110 HCV-Ab positive,in line with the rate of 78.57%.The positive detection rate of HCV-cAg with different HCV-RNA concentration was increased with the increase of HCV virus content,and the serum of different HCV-RNA concentration had no significant changes in HCV-Ab detection results.Conclusion The detection results of HCV-cAg had a high coincidence rate with HCV-RNA.Therefore detection of HCV-cAg can be as a complementary detec-tion of HCV-Ab,as the window period of HCV infection and infection in immunocompromised persons screening provides a simple,inexpensive method.At the same time it provides rapid screening for HCV infection provide diagnostic basis for those basic medical units who do not have the conditions for detection of HCV-RNA.

7

Cite

Share

Role of Maternal PAPPA and PIGF Levels at Second Trimester in the Prediction of Preeclampsia

Jinjing GUO

Journal of Modern Laboratory Medicine.2016;31(5):115-117. doi:10.3969/j.issn.1671-7414.2016.05.032

Objective To determine the PAPPA and PIGF levels in maternal serum at second trimester(15 to19 weeks)to e-valuate the role of these in the prediction of preeclampsia at last trimester.Methods Using premeasure postmeasure design, identified 204 singleton pregnancies within 15 to 19 weeks of gestation that followed 89 who had developed preeclampsia during 36 and 40 weeks,gestation at Weinan Municipal Maternal and Child Health Hospital at June 2013 and July 2015,115 with normotensive pregnant.PAPPA and PIGF concentration were determined.Results ①PAPPA and PIGF levels were significantly reduced in the preeclampsia groups compared to the normotensive pregnant group at second trimester (2.3μg/ml vs 4.6μg/ml,U=11.31,P=0.017;78 pg/ml vs 160 pg/ml,U=8.26,P=0.003).②PAPPA and PIGF levels were sig-nificantly reduced at last trimester compared to second trimester in the preeclampsia groups (1.6μg/ml vs 2.3μg/ml,U=9.41,P=0.011;56 pg/ml vs 78 pg/ml,U=6.77,P=0.023).③Receiver operating characteristic curve analysis showed that at second trimester(at 15 to 19 weeks)a cutoff value of 2.2μg/ml for PAPPA and a cutoff value of 75pg/ml for PIGF were able to predict preeclampsia with specificity,sensitivity and area under the curve (AUC)61.7%,87.8%,AUC 0.86(95%CI,0.83~0.91)respectively.Conclusion Measuring PAPPA and PIGF in the second trimester(at 15 to 19 weeks)may be useful in the prediction of preeclampsia.

8

Cite

Share

Clinical Significance of FLT3 in patients with AML

Jinwen HUANG ; Ruihong HUANG ; Guoqing HUANG

Journal of Modern Laboratory Medicine.2016;31(5):110-112. doi:10.3969/j.issn.1671-7414.2016.05.030

Objective To explore the expression of FLT3 on the cells from acute myeloblastic leukemia (AML)and its clinical signficance.Methods The expression of FLT3 from 80 AML cases was measured by FISH.All cases were indentified by karyotyping,40 cases were normal karyotye,and the other 40 cases were abnormal.Results The FLT3 expression level was different in different AML cases.The expression rate in normal karyotype cases was 5.0%,but in abnormal cases 30.7%,in refractory relapse cases 43.3%,in continual complete remission cases 4.5%.The patients’DFS (Disease-Free survival)and OS (overall survival)without FLT3 expression were 65% and 86% in 24 months’following investigation,but the patients’ DFS and OS with FLT3 expression were 20% and 40% respectively.The difference was distinct between them(P<0.05). Conclusion FLT3 can be thought as a new marker for worse prognosis of AML patients,and its expression level is associat-ed with maglignacy-grade.

9

Cite

Share

Research Advancement of TOF-MS for Analysising Protein Components in Patients with Diabetic Nephropathy Urine

Ning ZHANG ; Xuedong LU ; Zhenglin WU

Journal of Modern Laboratory Medicine.2016;31(5):160-164. doi:10.3969/j.issn.1671-7414.2016.05.048

TOF-MS has been more and more widely used in clinical and scientific research due to its advantages such as fast and easily operated.Diabetic nephropathy is the most common complications of diabetes.But clinical existing detection meth-ods to some extent have some hysteresis.The applications and developments of TOF-MS coupled with other proteomics technologies provided new ideas and methods for early diagnosis and prognosis of diabetic nephropathy.In this paper,the lat-est research results in nearest years about the application of TOF-MS to diabetic nephropathy will be reviewed.

10

Cite

Share

Trueness Controls of Clinical Laboratory to Assess Bias

Xiaoyan ZHANG ; Wei WANG ; Zhiguo WANG

Journal of Modern Laboratory Medicine.2016;31(5):147-149. doi:10.3969/j.issn.1671-7414.2016.05.044

Precision controls monitor assay random error (reproducibility),accuracy controls assess total error,both random error and systematic error,and trueness (bias)of an assay represents systematic error.A trueness control should be metro-logical traceable,ideally with a target value determined by use of a reference material or a reference method,without metro-logical traceable to a reference measurement system,patient test results for the same patient from different laboratories may not be comparable.A trueness control should be commutable and its analytical response to a reference method and a routine field method should be equivalent to that of a patient sample.And trueness control values should generally be set at medical decision levels and be prepared by the providers of reference materials and manufactured in the same fashion as a calibrator.

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share