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4th Mongolian External Quality Assessment Survey For Clinical Chemistry testing

Naran G ; Bayarzaya A ; Enkhzaya S ; Unurjargal SH ; Мinoru TAKAYANAGI

Health Laboratory.2013;1(1):5-10.

IntroductionWe organized the 4th Mongolian External Quality Assessment Survey (MEQAS) for Clinical Chemistry testing on basis of the Cooperation agreement between the Ministry of Health Mongolia and Sysmex Corporation in the establishment of the clinical chemistry external quality control, and reference laboratory system in 2011-2013.MethodIn 4th survey, the following survey material we used: Mtrol 1 (Level 1), Mtrol 2 (Level 2). To evaluate participant laboratories we divided into peer groups: full automated analyzer, semi automated analyzer and also divided analyzers by manufacturers and calculate standard deviation index (SDI), precision index (PI). We used absolute evaluation and scoring methods.ResultsThe number of participant laboratories increased in number by 133 instruments from 3rd MEQAS and became 139 instruments, but there were no significant improvements in most all items. The comparison transition of CV 4th MEQAS to 3rd MEQAS we found that there were not much differences between before the 3rd and 4th MEQAS, except some items like total protein, Lactate dehydrogenase (P-L), γ-Glutamyl transferase, potassium, bilirubin direct in which CV % have decreased in number by about 3% from 10% in both levels. We found out the difference results between full automated analyzer, semi automated analyzer groups and manufacturer analyzers and methods in both levels. To improve the results in the future to establish national standards for the reporting units and suggested reagents for clinical chemistry tests, which will be the directions for standardization.

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The diagnostic signficance of thyroid peroxidase

Unurjargal KH ; Batbaatar G ; Khadkhuu V

Health Laboratory.2013;1(1):11-14.

Background:In our country the thyroid diseases usually diagnosed using only laboratory indicators of thyroid hormons such as T3, T4, TSH and medical symptom. It means there are considerably late diagnosing of thyroid failure. In recently hypothyroidism and hypothyroidism are being diagnosed by specific thyroid antibodies which are instead during thyroid disorders in the worldwide. Although this diagnostic method is used in some laboratory in our country but their diagnostic significance have not yet been determined. It is important to lunce diagnostic methods for thyroid antibodies specially essential for endocrine doctors.Objective:To determine the diagnostic significance of thyroglobulin antibody and thyroid peroxidase antibodies in relation with thyroid disease.Research materials and methods:In this research of thyroid peroxidase antybody and thyroglobulin antibody, we use 20-64 age 23 patients who have hypothyrodism diagnostics, 36 patients how have hyperhotyrodism diagnostics, 30 patients who are relatively healthy and their disease was denied on symptom and laboratory test, total 89 people.Results:There were 57 female and 32 male from total 89 people investigated for this study. The traditional determination of thyroid hormones for diagnosis of thyroid diseases still have high diagnostic significance. The newly launched and used in our study anti-TPO and anti-Tg have very high significance for thyroid diagnosis. We found that anti-TPO antibody has primary information indicator and anti-Tg antibody is secondary information indicator for the diagnosis of thyroid disorder.Conclusion:1.Among 59 patients with thyroid diseases 89.8% (n=53) of them determined increased anti-TPO concentration and 61% (n=36) of them increased anti-Tg concentration. It shows increase of these antibodies have high diagnostic significance for thyroid diseases.2.For hyperthytiodism the anti-TPO has strong supportive information indicator (карра=0,64) and anti-Tg has primary information indicator (карра=0,84). 3.For hypothytiodism the anti-TPO has primary information indicator (карра=0,78) and anti-Tg has weak supportive information indicatot (карра=0,24).

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Results of chemical analysis of meals and food products being given to pupils of general education schools in the frame of the “School Lunch” program

Gantuya P ; Enkhjargal TS ; Ulziiburen CH

Health Laboratory.2013;1(1):15-18.

Background: The “School Lunch” program was initiated by the Government of Mongolia in 2006. The purpose of our study was to assess nutritious values of the meals and food products being given to pupils of general education schools in the frame of the program. Materials and methods: Nutritious values of 32 samples of school canteen meals and food products of 5 general education schools were determined using chemical analysis methods. Results: The average calorie content of the analyzed meals was 181.07-271.08 kcal, and the average content of vitamin C was 0.28-0.84 mg/g. The energy value of bakery products was high (110.97-436.23kcal) whereas their vitamin C content was low (0.34 mg/g). The calorie content in dairy products was reasonably high (46.17-52.85kcal), and the average level of calcium was 95.84-103.1mg/g, of iron was 0.7-1.7mg/g and of vitamin C was 0.28-0.84 mg/g. Conclusions
1.The study results highlight the necessity of broadening the variety of food products given to schoolchildren in the frame of the “Lunch” program.
2.Fruits and vegetables, the main source of minerals and vitamins, make a small percentage of the food products given to school children, and bakery products rich of calorie prevail in the menu.

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Result of microbiological analysis on Erdenet mining soil

Tuul N ; Dorjkhand B

Health Laboratory.2013;1(1):19-24.

1.Changes in microorganism’s quantity or activity can precede detectable changes in soil chemical properties, thereby providing an early sign of soil improvement or early warning soil degradation.2.Erdenet mining factory soil has approsintly 0.59*106 cell/g and control soil has 1.17*106 cell/g. Herein bacteria were 57%, actinomycete 31% and fungi 12%. But control soil bacteria were 83%, actinomycete 16% and fungi 1%.3.We have chosen deferent by morphology separate 29 cleanly cultures. In tolerance of heave metal, cultures which extracted from 1415 m level is tolerant against 6 mM of Fe, 5 mM of Pb, 3 mM of Mo and Cu, 1 mM of Cr

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Mutagenic activity of soil samples of Ulaanbaatar

Dorjkhand B ; Battsetseg CH

Health Laboratory.2013;1(1):25-26.

Abstract:The world population rising very fast and it has resulted in increasing the area of polluted soil, water and air. In order to cater to the demands of the people, the rapid expansion of industries, vehicles, etc. is necessary. But all of these developments make our world, especially urban area,heavily polluted. And many kind of contaminant substances showing bad effects to human health. Our research work aimed to evaluate Ulaanbaatar city soil contamination using sanitary microbiological and genetoxicological studies.The soils samples used in this study were collected from Ulaanbaatar city areas, from 5 points soil from the residential area of Ulaanbaatar; soil from city food market; soil from the area along the city highway; sludge of the city water treatment facilities; soil from the downtown. Genotoxicological effect of soil contaminant substances or ability to cause a mutation to living organisms was determined by Eims test and DNA damaging experiment on test strain Salmonella thyphimurium TA-100 (his G46, rfa B, bio pKm 101) and Escherichia coli (wp, uvr, pol). The result revealed that Ulaanbaatar city soil samples have inhibition effect to test strain growth rate.From our research work, we are making the following conclutions:1.Water and organic solvent solution of Ulaanbaatar soils have a high microbial growth inhibition rate. In particular, soil from the residential area has shown higher rate.2.Mutagenic activity of natural soil samples of Ulaanbaatar area was studied in bacterial test-systems. Soil samples revealed no mutagenic effect.

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Reticulocytes:analysis on automated analyzers and evaluation of analysis results

Khadkhuu V

Health Laboratory.2013;1(1):27-33.

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Are we ready for the clinical use of novel markers for early diagnosis of acute kidney damage?

Bayarjavkhlan CH ; Bayarmaa E ; Tuvshinjargal M ; Chuluunbaatar D ; Naran G ; Gelegjamts KH

Health Laboratory.2013;1(1):34-34.

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Study of growth gene of Mongolians

Enkhdelger M ; Oyungerel G ; Tselmen D ; Batchimeg B ; Bujin E ; Avirmed A ; Munkhbat B ; Amgalanbaatar D ; Munkhtuvshin N

Health Laboratory.2013;1(1):35-52.

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Blood hemolysis in a patient with pancreatitis

Enkhjargal TS

Health Laboratory.2013;1(1):53-55.

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Probiotic inhibitor activity against H.pylori

Mandkhai B ; Dugersuren J ; Bira N ; Sarantuya J

Health Laboratory.2013;2(2):5-10.

Background:The evidence that some strains of Lactobacillus and Bifidobacteriumare able to inhibit H.pylori growth through the release of bacteriocinsor organic acids. Therefore, it is important to in vitro study develop low-cost, large-scale, alternative probiotic to the at-risk population to prevent or decrease H. pyloricolonization.Methods:18 samples of gastric biopsies were cultured according to standard microbiological proceduresand were grown under microaerophilic conditions on selective Pylori agar. An in vitro disk diffusion assay was employed to assess the lactic acid bacteria LBO1, 2, 3, 4, 6, 7 cells and cell free supernatants (CFS) and bifidobacteria BFO1, BFO4 anti-H.pylori activity.Results: Ability of LBO1 strain to inhibit growth of H.pylori is 55,5% [95% CI 32.5-78.4], LBO-2 88,8% [95% CI 74.2-103.3], LBO-3 50%[95% CI 26.9-73.0]and LBO-4 38,8% [95% CI 32.5-78.4]. Then LBO 6 and LBO7 strains had no inhibitory activity against H.pylori. Average inhibition zone is 8-14mm (11,6 mm) for LBO1 strain, 10-16mm (11.3mm) for LBO2 strain , 8-12mm (10,2mm) for LBO3 strain and 10-12mm (10,5mm) for LBO4 strain.Inhibitioryactivity of Lactobacillus LBO1 supernatant against H.pylori accounts for 61.1% (n=11), LBO2 supernatant for 72,2% (n=13), and LBO3 supernatant for 33,3% (n=6) , while LBO4 supernatant inhibits only HP78 strain. LBO6 and LBO7 supernatants were both Lactobacillus LBO cultures. Average inhibition zone is 8-12mm (10 mm) for LBO1 supernatant, 10-16mm (11.3mm) for LBO2 supernatant , and 10-12mm (10,3 mm) for LBO3 supernatant.Bifidobacterium BFO1 strain was 83.3% inhibition activity. But BFO4 was not inhibit against all H.pylori strains.Conclusion:Lactobacillus LBO2 and Bifidobacterium BFO1 strains were isolatedfrom Mongoliantraditional fermented milk product were obtained more inhibition against H.pylori strains other LactobacillusLBO and Bifidobacterium BFO strains.

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