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Review the Function and Structure of Interleukin-35 and Its Function in Mediating Treg Cell Immuno-Suppression

Tianjin Medical Journal.2014;(12):1243-1245. doi:10.3969/j.issn.0253-9896.2014.12.026

Interleukin (IL)-35 is a new member of IL-12 family who is secreted by T regulatory cells to strongly sup?press immuno reaction directly or indirectly. There is a growing interest in the using IL-35 as a potential therapy for chronic in?flammatory , cancer and autoimmune diseases. Here we reviewed IL-35 and its function as a mediator of suppression.

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Evaluation the Effect of Dexamethasone Addition in Anesthesia on Postoperative Quality of Life after Extraction of Impacted Wisdom Teeth

Feng QIAO ; Hengxiang MA ; Rui DONG ; Jian ZHANG

Tianjin Medical Journal.2014;(12):1240-1242. doi:10.3969/j.issn.0253-9896.2014.12.025

Objective To investigate the effect of dexamethasone addition in block anesthesia before surgery on postoperative quality of life after extraction of impacted mandibular wisdom teeth. Methods Using a random number table, 120 cases of patients were divided into experimental and control groups, each group contains 60 cases whose mandibular wis?dom tooth will be extracted. Before the surgery, the experimental group patients perform local block anesthetic injection with 3 mL out of 5 mL mixture of 2%lidocaine 4 mL+dexamethasone 1 mL (5 mg) to block nerve. The control group was given block anesthesia of inferior alveolar nerve with 3 mL of 2%lidocaine block anesthesia alone. Improved postoperative symp?toms severity scale (PoSSe) was used to evaluate patients’quality of life after tooth extraction comprehensively and quantita?tively by analysis and comparison of the two sets of scores. Results The experimental group’s PoSSe scores were 18.059 1 ± 13.210 9, which was lower compared with control group (27.819 3 ± 15.716 9). In experimental group, diet, language, swelling, pain and daily points were lower than the control group (P<0.05). Conclusion Topical application of dexamethasone in mandibular wisdom tooth extraction reduces postoperative adverse reactions in a certain degree and im?prove postoperative quality of life.

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Chunhong LI ; Jie TIAN ; Hongling WANG ; Jihong ZHAO

Tianjin Medical Journal.2014;(12):1237-1239. doi:10.3969/j.issn.0253-9896.2014.12.024

Objective To explore potential related factors of left ventricular hypertrophy in maintenance hemodialy?sis (MHD) patients. Methods Sixty patients were enrolled in the study. Echocardiography was performed to exam LVH in?dexes. There are forty-three patients with LVH (LVH group), and seventeen patients without LVH (non-LVH group). Clini?cal data were collected to exam the levels of hemoglobin (HGB), creatinine (Cr), parathyrin (iPTH), albumin (ALB), triglycer?ide (TG), cholesterol(CHO), calcium(Ca), phosphorus(P), advanced oxygen protein products (AOPP) and C reactive protein (CRP). Results Compared with non-LVH group, age, BP, iPTH, AOPP were higher in LVH group patients while BMI and other biochemistry index does not show any statistical difference. Multiple linear stepwise regressions analysis shows that SBP, iPTH and AOPP are correlated factors to left ventricle mass index (LVMI). Conclusion LVH is prevalent in MHD pa?tients. BP, iPTH and AOPP are the risk factors for LVH.

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Treatment of Traumatic Pancreatitis after Hepaticoenterostomy in Congenital Biliary Dilatation

Peihong YAN

Tianjin Medical Journal.2014;(12):1223-1225. doi:10.3969/j.issn.0253-9896.2014.12.020

Objective To investigate the cause of postoperative pancreatitis in Congenital Biliary Dilatation(CBD), and to explore the preventive measures to decrease its occurrence. Methods Patients with CBD (n=22) were summarized during July 2010-March 2014 in Guiyang children's hospital, which include 8 cases of male, 14 cases of female;Cases that developed postoperative traumatic pancreatitis were taken as experimental group. Cases that did not developed it was used as control group. Ages are from 6 months old to 6 years and 9 months old. All patients were received cyst excision and hepatic duct-jejunum Roux- en- Y anastomosis. All postoperative patients are followed up regularly in clinic and by telephone. All followed up were from 3 months to 3 years with a comprehensive physical examination, routine blood test and urine amy?lase analysis. B-ultrasonic examination was used to understand pancreas recovery as well as the expansion of bile duct in the liver. Results Among the 22 cases, the postoperative 1st and 4th day blood amylase as well as 1st,4th and 7th day urine amylase in the experimental group are all higher than those in the control group with statistical difference ( P < 0.05). Patients in experiment group show low thermal and high leucocyte represent postoperative traumatic pancreatitis. All pa?tients were given trypsin inhibitor and anti-inflammatory treatment who were completely recovered after 4-9 days. All chil?dren growth developed normally, with only 2 cases of raised transaminase which were finally settled. Children showed no pan?creatitis and their ascending cholangitis, upper gastrointestinal angiography revealed no reflux gastritis. Conclusion The patients with CBD should receive surgery-cyst excision, hepatic duct-jejunum Roux-en-Y anastomosis early. During opera?tion, cysts decompression should be applied early. Besides these two procedures, interrupting pancreatic confluence and treat?ing pancreatic enzyme inhibitors postoperative can both help to decrease the occurrence of postoperative traumatic pancreatitis.

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Association of Nonalcoholic Fatty Liver Disease with Hypertriglyceridaemic Wasit Phenotype Complicated with Type 2 Diabetes Mellitus

Weiguang DING

Tianjin Medical Journal.2014;(12):1219-1222. doi:10.3969/j.issn.0253-9896.2014.12.019

Objective To investigate the association of nonalcoholic fatty liver disease with hypertriglyceridaemic wasit phenotype complicated with type 2 diabetes mellitus. Methods Base on whether plasma triglycerides concentra? tion≥1.7 mmol/L or not, waist circumference≥90 cm or not in men or≥85 cm or not in women, patients with type 2 diabe?tes mellitus (n=804) were divided into four groups:normal waist circumference with normal triglycerides, normal waist cir?cumference with hypertriglyceridemia, abdominal obesity with normal triglycerides and hypertriglyceridemic-waist pheno?type (HTWC). The four groups’clinical data were recorded. Body mass index (BMI), homeostasis model assessment insulin resistance (HOMA-IR) and nonalcoholic fatty liver disease fibrosis score (NAFLDFS) were calculated and the results were compared among four groups. Results BMI, waist circumference, systolic blood pressure, diastolic blood pressure, HOMA-IR, alanine aminotransferase, aspartate aminotransferase , γ-glutamyltransferase, serum uric acid,NAFLDFS and the inci?dence of NAFLD were all higher in the HTWC group compared to non-HTWC group (P<0.01). Logistic regression analysis revealed that besides BMI,γ-glutamyltransferase and serum uric acid,HTWC was also a risk factor for NAFLD in patients with type 2 diabetes mellitus(OR=1.986,95%CI:1.006-3.921). Conclusion HTWC is a risk factor for NAFLD in patients with type 2 diabetes mellitus. Screening and diagnosis of HTWC is helpful for reducing the occurrence of NAFLD and can slow its progress through controlling and interfering metabolic disturbance.

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Clinical Application of Platelet-Rich Plasma in Treatment of Deep GradeⅡBurn Wounds

Zhewei LIU ; Kaixin SU ; Jun CHEN ; Jian YAO ; Hui LI

Tianjin Medical Journal.2014;(12):1213-1215. doi:10.3969/j.issn.0253-9896.2014.12.017

Objective To evaluate the clinical effects of platelet-rich plasma in treatment of DeepⅡburn wounds. Methods Sixty eight cases of inpatients with Deep Ⅱburns were selected, whose age were from 1 to 65 years and their burn areas were between 5%~62%of total body surface area (TBSA). Deep gradeⅡburn of each sample was divided into two parts. Part A was the treatment group and part B was the control group.The burn wounds in the treatment group were treated with platelet-rich plasma and the counterpart in the control group were treated with SD-Ag. Healing time ,recovering rate and the frequency of dressing changes,frequency of changing the most innerlayer gauze and the rate of wound infection were also analyzed. Finally the laboratory abnormalities and adverse effect were monitored regularly. Results The healing time of the treatment group (16.5±3.1 ) d was shorter than that of the control group (19.5±3.8 ) d. The recovering rate of the treatment group was higher than that of the control group on the 14th and 17th day after treatment. There were statistically sig?nificant differences between these two groups (P<0.05). After two weeks’treatment, the internal and external dressing per?meability into wet gauze layers were 20.6 ± 1.7, which were significantly decreased than that in the control group 23.3 ± 5.9. The frequency of dressing changes was(7.2±1.1)times in treatment group versus(9.2±1.4)times in control group and the frequency of changing the inner most layer gauze was( 2.3±0.6)times in the treatment group versus(5.3±1.3)times in con?trol group. There were 5 inflammation reaction cases in the treatment group, but 13 cases in the control group. However, there was no statistic significance between the two group in the outcomes of bacterial culture, the laboratory abnormalities and the adverse effect. Conclusion Platelet-rich plasma can remarkably shorten the healing time,improve healing rate,re?duce frequency of dressing change and promote wound healing for deepⅡburn wound. PRP is a potential safety reagent in treating deepⅡburn wound.

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Relationship between Plasma sCD36 and Type 2 Diabetes Mellitus Combined with Nonalcoholic Fatty Liver Disease

Ling YANG ; Guoyu JIA ; Lu WANG ; Qiang LI ; Jie ZHANG ; Chunxiao XIE ; Fusheng DI

Tianjin Medical Journal.2014;(12):1209-1212. doi:10.3969/j.issn.0253-9896.2014.12.016

Objective To investigate the relationship between plasma soluble CD36 (sCD36) and nonalcoholic fatty liver disease (NAFLD) in patients combined with type 2 diabetes mellitus (T2DM). Methods Plasma levels of sCD36 were determined in normal control group (group A, n=39), patients of T2DM without NAFLD group (group B, n=39) and T2DM with NAFLD group (group C, n=59). Liver fat content (LFC) and nonalcoholic fatty liver fibrosis score (NFS) were calculated in group C. Glucose and lipid metabolic parameters, liver function parameters and inflammatory parameters were also detect?ed in all three groups. Variance analysis was applied to analyze the differences of the above parameters among three groups;Correlation analysis was used to analyze the relationship between sCD36 level and all the above parameters;Multiple step? wise regression analysis was applied to determine the influencing factors of sCD36 level in patients of group C. Results Plasma sCD36 (μg/L) levels in group B (3.87 ± 1.16) and group C (5.72 ± 1.79) are higher than that of group A (2.57 ± 0.93) (both P<0.01), and it is higher in group C than in group B (each P<0.05);Correlation analysis showed that sCD36 level was positively correlated with body mass index (BMI), waist, visceral adipose tissue,fast insulin (FINS), insulin resistance in?dex (HOMA-IR), free fatty acid (FFA), alanine transaminase (ALT), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), LFC and NFS (P<0.01 or P<0.05);Multiple stepwise regression analysis showed that FFA, LFC, TNF-αand IL-6 were in?fluencing factors of sCD36 level in patients of group C. Conclusion Plasma sCD36 level was related to fatty liver severity, liver injury and fatty liver fibrosis, it might be used as a plasma marker of T2DM combined with NAFLD. CD36 might con?tribute to the development of T2DM combined with NAFLD through inflammatory mechanisms.

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Awaken Effect of Naloxon in Dexmedetomidine-Anesthetized Mice

Cui LI ; Jianbo YU ; Fen ZHOU ; Huirong CHEN ; Man WANG ; Lirong GONG ; Rui MU ; Shuan DONG

Tianjin Medical Journal.2014;(12):1183-1185. doi:10.3969/j.issn.0253-9896.2014.12.009

Objective To investigate the awaken effect of naloxon on dexmedetomidine anesthetized mice and its mechanism. Methods Thirty Kunming mice of clean grade were randomly divided into 3 groups which included NAL group (Naloxon group), ATI group(Atipamezole group)and NS group (Normal Saline group). All groups were given dexme?detomidine 1 mg·kg-1 intraperitoneally. Naloxon 2 mg·kg-1, atipamezole 2 mg·kg-1 and normal saline 10 mL·kg-1 were ran?domly given intraperitoneally to the NAL, ATI and NS group respectively 90 minutes after dexmedetomidine administration. At timepoints prior to dexmedetomidine administration and 5, 15, 30, 60, 90, 95, 105, 120, 180 minutes after it, the sedative and analgesic effects besides recovery time (based on restore of righting reflex loss) were assessed. Results Sedation and analgesia effects became apparent within 5 minutes, and peaked at approximately 60 minutes then spontaneously recovered at 180 minutes after injection of dexmedetomidine. The sedative and analgesic effects were reduced in both ATI and NAL groups. Compared with ATI group, the sedation scores were higher at 95, 105 and 120 minutes after dexmedetomidine admin?istration than those in NAL group (P<0.05) but the scores were not statistically significant at 180 minutes between these two groups. Compared with NS group, the sedation scores were lower at time points of 95, 105, 120 and 180 minutes than those in NAL group (P>0.05). The analgesic scores were not statistically significant at time points of 95, 105, 120 and 180 min?utes between NAL group and ATI group, but they were lower in NAL group compared with NS group at timepoints of 95, 105 and 120 minutes (P>0.05). The recovery time in ATI and NAL group were shorter than that in NS group (F=1 793.368, P<0.05), but it showed no statistical difference between ATI group and NAL group (P>0.05). Conclusion Naloxone had a certain awaken effect on dexmedetomidine anesthetized mice.

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Effect of p53 on Protein Kinase R and Biological Behavior of Cervical Cancer HeLa Cells

Yuancai LUO ; Lu GUO

Tianjin Medical Journal.2014;(12):1168-1171. doi:10.3969/j.issn.0253-9896.2014.12.005

Objective To investigate the effects of p53 on expression and activity of protein kinase R (PKR) as well as biological characters of HeLa cells from cervical carcinoma patients. Methods Recombinant plasmid vector pEGFP-C1/p53 was constructed to over-express p53 then it was transfected into HeLa cells. Transcription levels of p53 and PKR mRNA were detected by reverse transcriptase polymerase chain reaction (RT-PCR) among pEGFP-C1/p53 transfection group, pEGFP-C1 transfection group and blank control group(only transfection reagent was added);Protein expression lev?els of p53, PKR, phosphated PKR(p-PKR)and phosphatedαsubunit of eukaryotic initiation factor 2(p-eIF2α)which is the downstream substrate of PKR were detected by Western Blot among three groups;Proliferation of HeLa cell were deter?mined by methyl thiazolyl tetrazolium(MTT)assay;Invasion of HeLa cell were determined by Transwell cell assay. Re?sults Recombinant plasmid vector pEGFP-C1/p53 was successfully constructed to overexpress p53;Transcription level of p53 and PKR mRNA in pEGFP-C1/p53 transfection group were higher than those in pEGFP-C1 transfection group and in blank control group (P<0.05),and there were no significant difference between their levels in pEGFP-C1 transfection group and in blank control group;Protein expression levels of p53, PKR, p-PKR andp-eIF2α in pEGFP-C1/p53 transfection group were higher than those in pEGFP-C1 transfection group and in blank control group (P<0.05),and there were no sig?nificant difference between those expression levels in pEGFP-C1 transfection group and in blank control group;MTT and Transwell cell results showed that proliferation and invasion of HeLa cells in pEGFP-C1/p53 transfection group were weaker than those in pEGFP-C1 transfection group and in blank control group (P<0.05),and there were no significant difference between proliferation and invasion of HeLa cells in pEGFP-C1 transfection group and in blank control group. Conclu?sion p53 can up-regulate the expression and activity of PKR, promote activation of PKR/eIF2αsignal transduction pas?sage and restrain cell proliferation and invasion of HeLa cells.

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Continuous Positive Airway Pressure Treatment for Epilepsy Complicated with Obstructive Sleep Apnea Syndrome

Yong ZHAO ; Baolin SHI ; Chunli WU ; Xiaoxia ZHANG ; Yanzhen ZHANG

Tianjin Medical Journal.2014;(12):1234-1236. doi:10.3969/j.issn.0253-9896.2014.12.023

Objective To investigate the effect of continuous positive airway pressure (CPAP) on seizure frequency in epilepsy patients complicated with obstructive sleep apnea (OSA). Methods Subjects were divided into CPAP group (20 subjects) and medication group (22 subjects) according to whether they can tolerate CPAP. CPAP group were treated with CPAP combined with antiepileptic drugs. Medication group were treated with antiepileptic drugs alone. Seizure frequency and apnea-hypopnea index (AHI) were compared between groups before and after 4 weeks and 24 weeks of treatment. Results Baseline seizure frequency and apnea-hypopnea index (AHI) were compared between groups(P>0.05). A sig?nificant reduction of seizure frequency was observed in CPAP group after 4 weeks and 24 weeks of treatment compared to that before treatment (P<0.01). In medication group, no significant difference in seizure frequency was noted betweem 4 weeks and 24 weeks after treatment compared to that before treatment(P>0.05). A significant reduction of AHI was ob?served in the CPAP group after 24 weeks of treatment compared to that before treatment (P<0.01), but no change of AHI by treatment was observed in medication group (P>0.05). Conclusion Treatment of OSA in patients complicated with epilep?sy may improve seizure control in short and long term, but longer observation time and more samples are needed for further research.

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