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Influence of Brain-activating Acupuncture on Cerebral Histomorphology in Rats with Focal Cerebral Ischemia and Reperfusion

Shu WANG ; Zhankui WANG ; Guangxia NI

Journal of Acupuncture and Tuina Science.2006;4(4):197-200.

Objective:To investigate the pathological changes following the focal cerebral ischemia and reperfusion in rats and the effect of acupuncture on them. Methods: A model of ischemia and reperfusion was established by thread occlusion, and the brain-activating acupuncture was applied, and then the morphological changes of the cerebral cortex on the ischemic side were observed under electron and light microscopes. Results:Cerebral ischemia and reperfusion could lead to the structural injury of neurons, gliacytes and capillary vessels in rat's brain, and acupuncture could improve the ultra-structural injury in the area around the cerebral ischemia. It was also found that acupuncture intervention at 3 hours following reperfusion was more ideal than that at any other time points. Conclusion: Acupuncture has a protective effect on the ultra-structural injury of neurons associated with focal cerebral ischemia and reperfusion in rats, and acupuncture given within 3 hours following reperfusion can produce a satisfactory effect.

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Functional Magnetic Resonance Imaging Study on Acupuncturing Shenmen (HT 7) and Sham Acupoint

Shangjie CHEN ; Jianwei LIU ; Bo LIU ; Shanshan WU ; Jun CHEN ; Pengcheng RAN ; Yuanchun XIAO

Journal of Acupuncture and Tuina Science.2008;6(4):242-244.

Objective: To observe the conditions of the activating areas in the brain evoked by needling Shenmen (HT 7) acupoint. Methods: Twelve healthy young volunteers were needled at Shenmen (HT 7) acupoint with rotating and twisting techniques. Functional magnetic resonance imaging (fMRI) was adopted to detect the brain activating areas, TR/TE/FA=3560 ms/50 ms/90°. After scanning, the images were dealt with SPM2. Results: Needling Shenmen (HT 7) primarily activated BA2 and BA1 of the gyms postcentralis in right-sided frontal lobe, BA47 of left-sided inferior frontal gyms and BA43 of left-sided gyms postcentralis, and also activated BA40 of inferior parietal lobule in right-sided parietal lobe, BA22 of gyrus temporalis superior in left-sided lobus temporalis, and BA40 of right-sided insula; however, the sham acupoints could not activate these nucleus. Conclusion: Needling Shenmen (HT 7) can activate the relevant functional areas in the brain.

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Effects of Herb-partitioned Moxibustion and Electroacupuncture on Serum Indexes of Intestinal Fibrosis in Rats with Crohn's Disease

Yanbo REN ; Cuihong ZHANG ; Caiping AN ; Huangan WU ; Jue HONG ; Lingxiang WU ; Jie LIU ; Xiaopeng MA ; Zhen WANG

Journal of Acupuncture and Tuina Science.2011;09(1):13-16. doi:10.1007/s11726-011-0460-1

Objective:To observe the impact of herb-partitioned moxibustion and electroacupuncture on hyaluronic acid(HA),procollagen Ⅲ(PC Ⅲ)and collagen Ⅳ(CⅣ)of intestinal fibrosis in rats with Crohn's disease(CD),and discuss the effect and the mechanism of acupuncture on treating intestinal fibrosis of rats.Methods:SD rats were randomly divided into control group,model group,herb-partitioned moxibustion group,electroacupuncture group and medicine group.The model of CD was developed by trinitrobenzene sulfonic acid.Masson stain was used to observe the collagen fibers hyperplasia in colon and radioimmunoassay was adopted to detect the content of HA,PC Ⅲ and C Ⅳ in serum.Results:Compared with the control group,the proliferation of colonic collagen fibers,as well as the HA,PC Ⅲ and CⅣ in serum increased in the model group.Compared with the model group,the expression of collagen fibers,serum HA,PC Ⅲ and C Ⅳ decreased in the herb-partitioned moxibustion group,electroacupuncture group and medicine group.The expression of collagen fibers serum HA,PC Ⅲ and C Ⅳ in the herb-partitioned moxibustion group were lower than in the electroacupuncture group and medicine group.Conclusion:Herb-partitioned moxibustion and electroacupuncture effectively improved the pathological state of intestinal fibrosis in rats with CD and reduced the content of HA,PC Ⅲ and C Ⅳ in serum.

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Moxibustion Therapy on Diabetic Peripheral Neuropathy in Rats for the Peripheral Neuroprotection

Haiyan YIN ; Jing WANG ; Linbo XU ; Yong TANG ; Dun WANG ; Yike XIE ; Shuguang YU ; Zhen WANG

Journal of Acupuncture and Tuina Science.2010;08(5):282-286. doi:10.1007/s11726-010-0427-7

Objective:To study the mechanism of moxibustion therapy on diabetic peripheral neuropathy for the peripheral neuroprotection.Methods:The DPN model was induced by intraperitoneal injection with streptozotocin (STZ).The rats were given moxibustion at the acupoint Yishu (Extra) and the acupoint Zusanli (ST 36).The treatment was carried out once a day and 15 minutes per acupoint,lasting for 56 d in total.The clinical effect of moxibustion was evaluated by detecting blood sugar,urine sugar,body weight and dietary intakes,as well as nerve conduction velocity with neuroelectrophysiological method.The structure variation of sciatic nerve was observed by HE staining and light microscopy,and the level of NGF in the sciatic nerve Was determined by ELISA.Results:Compared with the model group,the plasma glucose was significantly lower in the moxibustion group (P＜0.01),with significantly faster nerve conduction velocity (P＜0.01),more notably changes in pathological appearance (P＜0.01) and higher level of nerve growth factor (NGF)(P＜0.01).Conclusion:Moxibustion could improve the symptom and signs of peripheral neuropathy in rat models with DPN,which may relate to the increased NGF and enhanced peripheral nerve protection.

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Clinical Observation on Treatment of Facial Paralysis by Mild Moxibustion

Liwu ZHOU ; Quoqi HUANG

Journal of Acupuncture and Tuina Science.2009;7(4):218-220. doi:10.1007/s11726-009-0218-1

Objective: To observe the clinical effect of mild moxibustion for facial paralysis.Methods: One hundred cases of facial paralysis were divided randomly into two groups: 50 cases in the treatment group were treated by mild moxibustion, and 50 cases in the control group were treated by oral administration of Cobamamide Tablets. With ten days as one course of the treatments, two courses of the treatments were given. The therapeutic effects were assessed four weeks after treatments. Results: The total effective rate was 96.0% in the treatment group and 88.0% in the control group, and there was statistical difference in the therapeutic effects between the two groups (P<0.05). Conclusion: The therapeutic effect of mild moxibustion for facial paralysis is better than oral administration of Cobamamide Tablets.

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Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells

Xiaopeng MA ; Cuiying ZHAO ; Yunfei CHEN ; Huangan WU ; Zheng SHI ; Xian HONG ; Ling YANG

Journal of Acupuncture and Tuina Science.2008;6(6):331-333. doi:10.1007/s11726-008-0331-6

Objective: To observe the influence of moxibustion sertma of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method: The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results: The expression of bcl-2 mRNA and protein decreased, and the expression of Fas rnRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum (P<0.05). Conclusion: Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.

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Inhibition of P2X7 Receptor by Extracts of Chinese Medicine

Spielmann ANDREAS ; Quanbao GU ; Chunghui MA ; Chenggang HUANG ; Lijang XUAN ; Schwarz WOLFGANG

Journal of Acupuncture and Tuina Science.2008;6(5):286-288.

Objective: To investigate the influence of Acorus gramineus (Soland), a crude extract, SCP01, and a purified component, SCP02, and of Rosmarinus officinalis L., X0728 on human mast cells (HMC-1 Cell Line). Methods: Current-voltage of P2X7 receptors on human mast cell membrane activated by ATP was recorded by the whole-cell patch clamp technique. Results: The current at-100 mV mediated by P2X7was inhibited by (27.6±2.0) % in the presence of 40 μg/mL SCP01 and by (29.5±2.2) % in the presence of 40 μg/mL SCP02, which was identified as α-asarone. 42 μg/mL of the commercially available α-asarone inhibited the P2X7-mediated current by (52.2±2.0) %. In contrast to SCP01 and SCP02, 40μg/mL X0728 provoked stimulation of the current by (28.6±2.8) %. All effects were voltage-independent. Conclusion: The inhibition of P2X7by α-asarone will inhibit intracellular calcium increase and this may account for the inhibition of reported excitotoxic cell death. The pharmacological function of P2X7stimulation by X0728 needs further investigation.

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Acupuncture Treatment of 51 Cases of Menopausal Perspiration

Zejun HUO ; Wenbing ZHANG ; Guoqi HUANG

Journal of Acupuncture and Tuina Science.2004;2(4):27-28.

Fifty-one cases of menopause syndrome were randomly divided into three groups, 21 cases in the acupuncture and cupping group, 10 cases in the acupuncture group, and 20 cases in the ear acupuncture group, and were respectively treated by acupuncture plus cupping therapy, acupuncture,and ear acupuncture. The total effective rate was respectively 80.9%, 20.0% and 80.0%. The therapeutic effect was better in the treatment of menopause syndrome by acupuncture plus cupping therapy than by single acupuncture treatment or ear acupuncture (P＜0.05).

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Treatment of 38 Cases of Allergic Rhinitis by Ventilating and Dredging the Nasal Passage

Xiao YANG ; Le KUAI

Journal of Acupuncture and Tuina Science.2004;2(1):49-50.

Thirty-eight cases of allergic rhinitis has been treated by puncturing the points of Yintang (Ex-HN 3), Yingxiang (LI 20), Feishu (BL 13), Fengchi (GB 20), Shangxing (GV 23), Hegu (LI 4) and so on, combined with massaging the head, face and trunk. The result showed that cure occurred in 16cases, marked effectiveness in 18 cases and ineffectiveness in 4 cases.

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Observation on Clinical Therapeutic Effect of Electroacupuncture plus Tuina on 90 Cases of Scapulohumeral Periarthritis

Huanlan LIU ; Le KUAI

Journal of Acupuncture and Tuina Science.2003;1(6):31-33.

Ninety cases of scapulohumeral periarthritis were treated by electroacupuncture plus Tuina and the effective rate was 94.4%. The effective rate was 81.9% in the control group (treated by Frozen Shoulder Decoction added by other herbal drugs) of 83 cases. The result indicated that the treatments of the two groups were effective, but the therapeutic effect of the treatment group was better than that of the control group. In the treatment group, the cure rate was higher, the duration of treatment was shorter and the sequelae of conglutination and rigidity of the shoulder joint, muscular atrophy and so on were reduced.

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