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Clinical significance of multiple serum tumor markers combined detection in the diagnosis of lung cancer

Chongqing Medicine.2013;(31):3764-3765. doi:10.3969/j.issn.1671-8348.2013.31.014

Objective To study the clinical significance of Carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CY-FRA21-1) ,neuron specific enolization enzyme (NSE) combined detection in the diagnosis of lung cancer .Methods Using the Chemiluminescence to detect the level of CEA ,NSE and CYFRA21-1 in 61 patient with lung cancer (lung cancer group) and 38 pa-tients with benign diseases(control group) .All datas were analyzed with t test .Optimal cut-off point and diagnostic sensitivity ,spe-cificity were obtained by receiver operating characteristic curve (ROC) .Results The level of serum CEA ,NSE and CYFRA21-1 in Lung cancer group was significantly higher than that in control group (P<0 .05) .The expression level of three tumor markers was no statistically significant difference in the different pathological types of lung cancer (P>0 .05) .The cut-off point of CEA was 3 .50 ng/mL ,sensitivity and speciality were 52 .5% ,94 .7% respectively .The cut-off point of CYFRA21-1 was 5 .21 ng/mL ,the sensitiv-ity and specificity were 67 .2% ,93 .1% .The cut-off of point NSE was 18 .09 ng/mL ,the sensitivity and specificity were 77 .0% , 52 .6% .The three combined detection sensitivity was 96 .7% ,significantly higher than separate one(P<0 .05) .Conclusion The three tumor markers(CEA ,CYFRA21-1 ,NSE) might be valuable in the diagnosis of lung cancer .The combined detection could be used as a better pattern for screening lung cancer .

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Association of angiotensinogen gene M235T polymorphisms with essential hypertension：a meta-analysis

Xuming ZHU ; Guoqiang XIE ; Jian ZHANG ; Weifeng SUN ; Guoqian CHEN

Chongqing Medicine.2013;(31):3759-3763. doi:10.3969/j.issn.1671-8348.2013.31.013

Objective To evaluate the association of angiotensin (AGT ) gene M235T polymorphism with essential hypertension (EH) .Methods The literatures examining the association of the M 235T polymorphisms with EH were selected in electronic data-bases .Using RevMan5 .0 ,heterogeneity and pooled effect sizes were calculated .Results Totally ,29 articles ,with 5 971 cases and 6 443 controls were finally included in this study .The odds ratio (OR) value of TT+ TM∶MM genotype was 1 .13 (95% CI 1 .00-1 .25 ,P=0 .04) in worldwide populations .Subgroup analysis showed that the OR values of TT ∶MM genotype were 1 .13 (95% CI 0 .90-1 .42 ,P=0 .28) in subjects published before 2008 and 1 .29(95% CI 0 .94-1 .75 ,P=0 .11) in those after 2008(year of 2008 included) .The OR values of T ∶M in Han population was 1 .24 (95% CI 1 .01-1 .53 ,P=0 .04) .Conclusion TT+ TM genotype of the M235T polymorphisms is associated with EH in worldwide populations ,and T allele is also correlated with EH in Han popu-lation .

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Detection of nanobacteria in serum,urine and calculus of patients with upper urinary calculi

Hongwei SU ; Tongliang CHEN

Chongqing Medicine.2013;(31):3754-3756. doi:10.3969/j.issn.1671-8348.2013.31.011

Objective To investigate the infection status of nanobacteria on patients with upper urinary calculi and healthy sub-jects ,and analyze the role of nanobacteria in the formation of upper urinary calculi .Methods The serum ,urine and calculus of 42 patients with upper urinary calculi were investigated by polymerase chain reaction (PCR) and by bacteria cultivation with 10% γ-FBS and PMBI 1640 .The resulting PCR products were sequenced for further comparison with the reported sequence in gene bank . The serum and urine from 30 healthy adults were used as controls .Results After 4 to 6 weeks′culture ,the white or yellow precipi-tate was visible at the bottom of the tube .The positive rate of PCR was 90 .4% in calculous patients urine and 6 .7% in healthy a-dults urine ,as the positive was 92 .8% and 6 .7% in serum .which there is significant difference (P<0 .01) .The positive rate of the nanabactria in urinary calculi was 95 .2% .The coincidence rate was 98 .72% between the PCR products and the reported sequence in gene bank .Conclusion Nanobacteria are widely existed in the serum ,urine and calculus of the patients with upper urinary calcu-li ,this indicate that the nanobacteria might be have the most important influence on the formation process of urinary calculi .

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Clinical study of Heweijiangni granule on gastroesophageal reflux disease

Weiyao XIE ; Anqing JING

Chongqing Medicine.2013;(33):4022-4023. doi:10.3969/j.issn.1671-8348.2013.33.017

Objective To explore the clinical effect of Heweijiangni granule on gastroesophageal reflux disease (GERD) .Methods 80 patients were randomly divided into two groups ,40 cases in each group .The treatment group were treated with Heweijiangni granule ,while the control group were treated with western routine therapies .The courses of treatment in two group were 8 weeks . Results The total symptom effective rate of treatment group was 92 .50% and the control group was 62 .50% ,differences between the two groups have statistical significance (P<0 .05) .But no significant difference was found between the two groups on esophage-al mucosa under gastroscopy(P>0 .05) .Conclusion Heweijiangni granule can improve the main symptoms such as heartburn ,pan-tothenic acid ,chest pain and regurgitation of GERD ,and the effect is very significant .

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Analysis of bronchial dilation test and bronchial provocation test for 650 cases of asthma patients

Wenhong PENG ; Xin HONG ; Yutian BI ; Wenhui LEI

Chongqing Medicine.2013;(33):4019-4021. doi:10.3969/j.issn.1671-8348.2013.33.016

Objective To analyse the airway reversibility and variability for bronchial asthmatic patients .Methods The status of test positive ratio and combined airflow limitation were analysis for 650 Clinical diagnosed asthmatic patients .527 cases were given bronchial dilation test and 123 cases were given provocative test .Results Positive ratio of 527 cases bronchial dilation test was 41 . 4% .Positive ratio of 123 cases provocative test was 52 .0% .The airflow limitation(FEV1/FVC% <70% ) ratio of total 650 cases was 34 .0% .In the 193 cases of combined airflow limitation patients which was given bronchial dilation test ,the positive ratio of bronchial dilation test was 61 .7% .Conclusion Bronchial dilation test need repeat because of its less sensitivity .The positive ratio of provocative test is not high ,so the patients of provocative test negative couldn′t exclude the diagnosis of asthma .The ratio of bronchial dilation test was high for combined airflow limitation asthmatic patients .Its may clew that the patients combined airflow limitation may be exacerbation and need actively therapy .

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Effects of different dose of edible liquor on cardiac structure and function in rats

Hui JIANG ; Hong TIAN

Chongqing Medicine.2013;(32):3926-3928. doi:10.3969/j.issn.1671-8348.2013.32.030

Objective To investigate the effects of different dose of edible liquor on cardiac structure and function in rats ,and explore its underlying mechanisms .Methods 54 male SD rats were randomly divided into saline group ,alcohol group ,liquor group , each group were further divided into low-,middle-and high-dose groups .After 12 weeks of feeding ,left ventricular pressure was de-termined ,and left ventricular pressure amplitude and indexes of HW /BW were calculated .Myocardial fibrosis was observed by Masson staining ,AngⅡ content of myocardial tissue were detected by ELISA ,and ACE and ACE2 mRNA expressions were detec-ted by RT-PCR ,respectively .Results Left ventricular pressure amplitude and HW/BW index were increased by both liquor and al-cohol .The myocardial cell turbidity ,degeneration ,hyperplasia of fibrous tissue change were gradually worsened ,and the AngⅡ con-tents and the ACEmRNA expressions of the myocardial tissue were also increased by both liquor and alcohol .The ACE2 mRNA ex-pression of the myocardial tissue were higher by low-dose liquor and alcohol ,while the ACE2 mRNA expression of the myocardial tissue were lower by middle-and high-dose liquor and alcohol .Conclusion Long-term alcohol consumption can damage the balance of cardiac ACE and ACE2 ,causing the increase of ACE ,AngⅡ and the decrease of ACE2 ,resulting in myocardial fibrosis ,whose degree is related to the amount of alcohol consumption and drinking history .

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Prediction analysis of ametropia in children aged from 5 to 13 years old

Danni ZHAO ; Pinghua LI

Chongqing Medicine.2013;(31):3798-3800. doi:10.3969/j.issn.1671-8348.2013.31.027

Objective To investigate the characteristics of children ametropia in the development process ,predict and analyze the refractive state with the measurement data of refractive factors on the children aged from 5 to 13 years old .Methods 461 children (922 eyes) ,aged from 5 to 13 years old ,were selected from outpatients who went to ophthalmology clinic from January 2011 to September 2012 .The static diopter was measured by the optometry with the continuous mydriasis of 5 days using the ophthalmic gel of 1% atropine .And the biological measured values of refractive factors were obtained by the A type ultrasonic and keratome-ter .Meanwhile ,the parameters including the height (H) ,weight and the dominant eye were recorded to analyze the characteristics of children ametropia in the development process ,and then the equation between the diopter and the correlative factors including the measurement data of refractive factors and growth factors was established by the stepwise multiple regression analysis .Results (1) The myopia degree of the right eye static diopter was significantly deeper than the left (P<0 .05) .In the group in which the left eye was dominant ,the difference of the left eye and right eye refractive factors was not statistically significant (P>0 .05) .In the group in which the right eye was dominant ,the difference of the left eye and right eye refractive factors was not statistically significant (P>0 .05) except the instance in which K1 of the right eye was bigger than the left eye(P<0 .05) .(2) In the period of 5 to 13 years old ,the myopia developing trend of girls was earlier than boys .(3) The ocular axis length(AL) and H development had a close relationship ,and the correlation equation of them was AL =16 .135+0 .052H(r=0 .993) .(4) The multiple linear regression equation for the relationship between the static diopter (D) ,ocular AL and mean value of the corneal curvature (K) was D=84 .271-0 .937K -1 .878AL(r=0 .871) .Conclusion The ocular AL and H development of children have a close relationship .The static diopter(D) of the children aged from 5 to 13 years old can be predicted by the ocular axis length(AL) and corneal curvature(K) , w hich can be used to prevent myopia reasonably .

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The study of prospective space-time scan analysis for disease early warning in health emergency command and decision-making syste

Xianke WANG ; Ronggang YANG

Chongqing Medicine.2013;(31):3795-3797. doi:10.3969/j.issn.1671-8348.2013.31.026

Objective To explore the feasibility of using the prospective space-time analysis and early warning in health emer-gency command system in Chongqing .Methods Prospective space-time scan analysis method was used for the early detection of disease outbreaks in mumps patients from 5 districts(Jiangbei ,Shapingba ,Dadukou ,Jiulongpo ,Nan′an ,) in Chongqing in 2006 ,then verified the aggregation using the actual reported mumps outbreaks .Results The results showed that 3 early warning signals at October 9th ,November 13th ,December 4th ,involving a total of 5 locations(streets/towns) .The strongest warning happened on Oc-tober 9th in Zengjia town of Shapingba district ,and Jingfeng town of Jiulongpo district ,the recurrence interval was 1 916 495 550 992 471 years .There were 2 mumps outbreaks have been reported in the surveyed areas .Both the the 2 outbreaks were detected by the prospective space-time scan statistic 5-6 days early than the actually occurred time .Conclusion The prospective space-time scan analysis method could be applied to the disease monitoring and early warning in health emergency command decision-making system in Chongqing .

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Effects of zinc finger protein A20 on the inflammatory reaction in rats with peritoneal dialysis-related acute peritonitis

Xunliang ZOU ; Juzhen YAN ; Gang XU ; Ping WU

Chongqing Medicine.2013;(31):3788-3791. doi:10.3969/j.issn.1671-8348.2013.31.024

Objective To explore the effects of zinc finger protein A20(A20) on the expression of CD40 and the phosphorylation of IκBαas well as the local inflammatory reaction of abdominal cavity in Sprague Dawley (SD) rats with peritoneal dialysis-related a-cute peritonitis induced by lipopolysaccharide (LPS ) .Methods 24 male SD rats were equally randomized to four groups (n= 6 , each) .Control group:injected with 4 .25% dextrose peritoneal dialysate(PDF) via abdominal cavity (90 mL/kg);LPS group :injec-ted with LPS(1 mg/kg) via abdominal cavity 4 hour later followed by PDF injection ;transfection A20 plasmid group and empty plasmid group:after transfer pGEM-T easy-A20 or pGEM-T easy plasmid via intraperitoneally using an ultrasound-microbubble-mediated system for 3 days ,then injected with LPS and PDF via abdominal cavity .The rats were killed 4 hours after PDF injection . Peritoneum tissue was stained using Masson and HE .Leucocytes count in abdominal dropsy was performed .The proteins expres-sion of A20 ,p-IκBα,IκBα,and CD40 in peritoneum tissue were analyzed by western blot ;the expression of CD40 mRNA in peritone-um tissue were determined by RT-PCR;IL-6 level in abdominal dropsy was determined by ELISA .Results LPS could induce the protein expression of A20 in rats peritoneum tissue .Compared with LPS group and empty plasmid group ,the degree of edema ,in-flammatory cells infiltration ,and the ratio of p-IκBα/IκBα,mRNA and protein expression of CD40 in rats peritoneum as well as leu-cocyte counts and IL-6 level of abdominal dropsy were also significantly decreased in transfection A20 plasmid group(P<0 .05);meanwhile ,compared with control group ,that of the ratio of p-IκBα/IκBαand IL-6 level in transfection A20 plasmid group were no significant difference(P>0 .05) ,but the mRNA and protein expression of CD40 were significantly higher than that of control group (P<0 .05) .Conclusion Over expression of A20 in SD rats peritoneum tissue could down-regulate the inflammatory reaction in peritonitis induced by LPS ,which might be involved in modulating the expression of associated functional protein during LPS signal pathw ay .

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Clinical characteristics analysis of pleural effusion in 117 children

Luliang CAI

Chongqing Medicine.2013;(31):3786-3787. doi:10.3969/j.issn.1671-8348.2013.31.023

Objective To study the etiology and clinical features of pleural effusion in Children .Methods The clinical data of 117 cases who diagnosed with pleural effusion in children were analyzed retrospectively .Results In the study ,infectious pleural ef-fusion that were 97 cases(82 .9% ) ,in which 79 cases caused by suppuration(67 .5% ) ,9 cases caused by mycoplasma(7 .7% ) ,6 ca-ses caused by virus (5 .1% ) ,3 cases caused by tuberculous-empyema (2 .6% ) .Non-infection cause included 20 cases (17 .1% ) ,in which 6 cases of nephrotic syndrome ,4 cases of malignant tumor(1 case of lung cancer ,1 case of lymphoma ,1 case of leukemia ,1 case of nephroblastoma and lung metastasis) ,4 cases of Kawasaki disease ,2 cases of acute glomerulonephritis ,2 cases of industrial vinegar concentrate poisoning ,2 cases of hemolytic uremic syndrome ,1 case of hypereosinophilic syndrome .There were 83 cases cured in 117 cases of pleural effusion ,26 cases left hospital with getting better ,7 cases of no cure(4 cases of malignant tumor ,1 case of nephrotic syndrome ,2 cases left hospital automaticly )and 1 case of death .Conclusion Infection is the main cause of pleural effu-sion in children ,it should be pay attention to pleural effusion in children caused by various diseases in the process of clinical diagno-sis and treatment .

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