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The expression and clinical signification of tM2-PK in colorectal cancer

Acta Universitatis Medicinalis Anhui.2014;(2):236-239.

Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry ( IHC ) methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects ( P<0.05 ) . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects ( P<0.05 ) . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels (r=0.357,P=0.003). Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.

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Application of 23 G transconjunctival sutureless vitrectomy irrigation system in surgery for post-vitrectomy cataract

Yong WANG ; Shouling LI ; Bingying ZHAO

Acta Universitatis Medicinalis Anhui.2014;(1):117-119.

A retrospective study included 30 cataract cases (30 eyes) with history of vitrectomy. Phacoemulsifica-tion or small incision non-phacoemulsification was performed with vitreous irrigation of 23G TSV. Observe the visual acuities, intraoperative and postoperative complications. Posterior capsule rupture intraoperatively occurred in one patient with phacoemulsification. Three patients suffered intraocular hypotension postoperatively, including one with choroidal detachment, and the other two probably with leakage from incisions. However,the intraocular pressure rised to normal after treatment. Corneal edema and aqueous flare postoperatively occurred in four patients with hard nucleus cataract and three patients with diabetic retinopathy. There were no serious complications occurred in all patients, such as sclera collapse, nucleus sinking into vitreous chamber and bleeding in epichoroidal space. The visual acuities were improved after operation in twenty-eight patients.

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Value of contrast-enhanced ultrasound and color Doppler in diagnosing portal hypertension esophageal varices

Yayun CUI ; Ling WANG ; Chaoxue ZHANG

Acta Universitatis Medicinalis Anhui.2014;(1):96-99.

Objective To discuss the value of contrast-enhanced ultrasound and color Doppler in diagnosing esoph-ageal varices( EV) among patients suffering from portal hypertension. Methods The control group consisted of twenty nine patients without EV. Sixty patients with EV diagnosed by endoscopy were divided into two groups equal in number according to grade of EV. One being mild,the other was moderate to severe. All patients underwent color Doppler flow imaging and contrast-enhanced ultrasound, and the dynamic angiography data were collected. Time-in-tensity curves were drawn by software. The quantitative parameters including arrival time of hepatic artery ( HAAT) ,hepatic vein ( HVAT) and portal vein ( PVAT) ,damping index ( DI) of hepatic vein and portal vein ve-locity( PVV) were compared. Results The difference of PV-HV, PV-HA, PVV, DI showed statistically signifi-cant among the three groups(F=72.63,14.97,6.71,13.74,P<0.01). A comparison of the control and moderate to severe group among the above four parameters was statistically significant (P<0.01). PV-HV, PV-HA, DI in moderate to severe group showed statistically significant compared with mild group (P<0.01),however there exis-ted no significant difference grouping PVV between the two groups. Mild group contrasted to the control group,the differences of PV-HA were statistically significant (P<0.01), whereas PV-HV, DI, PVV displayed no significant difference. Conclusion Contrast-enhanced ultrasound and color Doppler are helpful in diagnosing EV,which is ex-pected to become a new noninvasive method.

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Expression and significance of MMP-14 and EMMPRIN in human oral squamous cell carcinoma

Ye TAO ; Lingda ZHANG

Acta Universitatis Medicinalis Anhui.2014;(1):81-84.

Objective To investigate the expression and significance of MMP-14 and EMMPRIN and the correlation between them in oral squamous cell carcinoma ( OSCC) . Methods The expression of MMP-14 and EMMPRIN mR-NA in 43 cases of OSCC and 11 cases of normal oral tissue were detected by RT-PCR. Results The expression of MMP-14 and EMMPRIN mRNA in OSCC was significantly higher than those in normal oral tissue ( P <0.05 ) . Compared with those without lymph node metastasis in OSCC, the expression of MMP-14 and EMMPRIN mRNA with lymph node metastasis was significantly higher ( P <0.05 ) . Furthermore, the expression of MMP-14 and EMMPRIN was significantly associated with pathological stage and the lymph node metastasis,but neither of them was correlated with sex and age. Correlation analysis showed that MMP-14 expression level was positively related to EMMPRIN level(r=0.801,P<0.01). Conclusion The expression of MMP-14 and EMMPRIN in OSCC is signif-icantly correlated with occurrence, progress and metastasis of OSCC. Therefore, the combination of detecting the expression of MMP-14 and EMMPRIN mRNA may evaluate the degree of malignancy and predict the prognosis of patients with OSCC.

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Comparison of puncture in different positions with combined spinal and epidural anesthesia in obese parturients for cesarean section

Qingmei ZHANG ; Xiaoqiong XIA ; Xianzhong ZHA

Acta Universitatis Medicinalis Anhui.2014;(1):110-112.

Objective To observe the influence of combined spinal and epidural anesthesia( CSEA) of different po-sitions on the anesthesia effect, anesthesia operation and hemodynamic index during cesarean section of obese par-turients. Methods Eighty obese parturients[BMI≥30] undergoing cesarean section were randomly divided into two groups, that was group A and B, with 40 cases each. In group A, the parturients were required to undergo lat-eral decubitus position with 0.5% ropivacaine hydrochloride of 2. 4 ml. In group B, the parturients were required to undergo sitting position with the same ropivacaine hydrochloride as group A. The following data was recorded, which were the percentage of one-attempt's successful epidural needle placement, the anesthesia level,the anesthe-sia effect and the hemodynamic change. All the complications were also observed,such as the supine hypotension syndrome during operation,headache after spinal anesthesia and postoperative spinal nerve stimulation. ResultsThe difference of anesthetic effect between the two groups was insignificant in statistics while group B's rate of one-attempt successful puncture was higher than gruop A ( P <0.05 ) . The difference of hemodynamic index between them was significant in statistics ( P<0.05 ) with group B's rate of supine hypotension syndrome lower than group A. Conclusion Both body positions have the same anesthtic effect for obese parturients during cesarean section while the puncture operation of sitting positon is easier than lateral decubitus position and the hemodynamic change is also more stable.

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Expression and clinical significance of Her-2/neu,MMP-2 and Leptin in serum and tumor tissue of gastric carcinoma

Ershu GE ; Yi LIU

Acta Universitatis Medicinalis Anhui.2014;(1):76-80.

Objective Detected Her-2/neu, matrix metalloproteinases-2 (MMP-2) and Leptin in stomach cancer serum and tissue, discussed their roles in occurance and development of gastric cancer. Methods Detected the ex-pression of Her-2/neu, MMP-2 and Leptin in serum by ELISA, and detected the expression of Her-2/neu, MMP-2 and Leptin in stomach cancer tissue by IHC. Results The positive rates of Her-2/neu, MMP-2, Leptin in gas-tric cancer serum were higher than the positive rates in control group ( P<0.05 ) . The positive rates of Her-2/neu, MMP-2, Leptin in gastric cancer tissues were higher than the positive rates in adjacent tissues (P<0.05 ). The positive expressions of two tests had consistency. In tissues, the positive expression rates of Her-2/neu,MMP-2 and Leptin were correlated with the depth of invasion,lymph node metastasis, clinical stage(P<0.05). Their ex-pression was not correlated with sex, age, size, site of tumor and the degree of differentiation. In serum, the posi-tive expression of Her-2/neu was correlated with the depth of invasion, lymph node metastasis, clinical stage( P<0.05 ) . The positive expression of MMP-2 was correlated with lymph node metastasis ( P<0.05 ) . The positive ex-pression of Leptin had no significant correlation with pathological features. Conclusion Her-2/neu,MMP-2 and Leptin promote the occurrence, development and transfer process of gastric cancer. It can be used for clinical diag-nosis, guide the targeted therapy and provide scientific and reasonable reference for gastric cancer prognosis.

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Effects kui-yang-fang on TFF1,EGF and EGFR in acetic acid induced gastric ulcers of rats

Na LIU ; Wei JIANG ; Jing TANG

Acta Universitatis Medicinalis Anhui.2014;(1):51-54.

Objective To investigate the effects of kui-yang-fang on trefoil factor 1 ( TFF1 ) , epidermal growth fac-tor ( EGF) and epidermal growth factor receptor( EGFR) in acetic acid induced gastric ulcers of rats. Methods50 rats were randomly divided into five groups. The rats of the gastric ulcer model were used by the icy acetic acid. After drug treatment for 14 days,the rats gastric mucosa was collected to compare to the ulcer index,then check the content of TFF1,EGF and EGFR with RT-PCR,and observe TFF1,EGFR with immunohistochemical staining. Re-sults Compared to the control,the levels of TFF1 and EGF in model group decreased, and the difference was not statistically significant. The EGFR level of model group was higher than that of normal group with no statistically significant difference. But the increase of kui-yang-fang groups and ranitidine group was more obvious,the difference was statistically significant (P<0.01). Compared to the model group,kui-yang-fang and ranitidine groups signifi-cantly elevated the level of TFF1,EGF and EFR in gastric ulcer,the difference was statistically significant ( P<0.01 ) . Immunohistochemical staining showed that TFF1 and EGFR expression in kui-yang-fang and ranitidine groups were significantly increased,the difference was statistically significant (P<0.01), but the increase of kui-yang-fang of high-dose group was more obvious. Conclusion One of the possible mechanism is kui-yang-fang pro-motes expression of TFF1,EGF and EGFR to protect and repair gastric tissue.

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Detection of phenotype and analysis of genotype in vancomycin-resistant Enterococci

Xiajia ZHANG ; Jilu SHEN ; Weihua JIA

Acta Universitatis Medicinalis Anhui.2014;(5):662-664.

Objective To research the resistant characteristics, genotype and prevalence of vancomycin-resistant Enterococci( VRE) . Methods K-B disc diffusion method was used to determine the susceptibility, minimum inhibi-tory concentration ( MIC) for vancomycin of VRE was detected by E-test method;VRE was then subjected to PCR for resistance related genes;6 strains sequencing results of PCR product were contrastively analyzed the amino acid sequence by BLAST. Results 6 VREFm strains were found from 193 strains enterococci;6 VREFm strains were completely resistant to high unit gentamicin, ampicillin, ciprofloxacin, teicoplanin,but were sensitive to linezolid and macrodantin,MIC for vancomycin was more than 256 mg/L;Genotypes were vanA;Amino acids in vanA gene have changed with Asn83→Asp( AAC→GAC) . Conclusion VRE in our hospital is mostly multi-drug resistant , which bringS difficulty in clinical treatment for its infection. So the hospital should strengthen the prevention and monitoring, to prevent the spread of VRE strains in the hospital and popular.

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Comparison of 60 multiple fractured ribs patients treated with surgical and non-surgical treatment

Xiaosong YANG ; Zaicheng YU

Acta Universitatis Medicinalis Anhui.2014;(5):689-691.

To compare the effect of surgical and non-surgical treatment for multiple fractured ribs retrospectively, with 30 cases in each group. Patient's length of stay, visual analogue scale ( VAS) pain, independent ambulation time, cost of hospitalization, thoracoplasty degree and the lung ventilation function were evaluated. The results showed that VAS declined obviously in patients of surgical group, length of stay and independent ambulation time were shorter. Compared to the non-surgical group, the maximal voluntary ventilation increased significantly and ref-erral rib reduction was significantly better, but cost of the surgical group was higher. It's an effective treatment for using nickel titanium memory alloy embracing fixator in the treatment of multiple fractured rips, and is worthy of clinical application.

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Precocious puberty girls relationship between depression and E2, FSH, LH levels

Wei ZHANG ; Liqi YANG

Acta Universitatis Medicinalis Anhui.2014;(5):687-688.

56 girls with central precocious puberty were divided into the depression group and non-depression group according to the short mood and feelings questionnaire;then the levels of basic estradiol ( E2 ) , follicle stimu-lating hormone ( FSH ) and luteinizing hormone ( LH ) at various time points were compared between the two groups. The E2 level of the non-depression group was higher compared with the depression group ( P <0.01 ) . However, the significant difference of FSH and LH between the two groups was not discovered (P>0.05). The re-sults above demonstrated a negative correlation between depression and the E2 level of precocious puberty girls.

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