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The synergistic regulatory effect of Runx2 and MEF transcription factors on osteoblast differentiation markers.

Jae Mok LEE ; Towia A LIBERMANN ; Je Yoel CHO

Journal of Periodontal & Implant Science.2010;40(1):39-44. doi:10.5051/jpis.2010.40.1.39

PURPOSE: Bone tissues for clinical application can be improved by studies on osteoblast differentiation. Runx2 is known to be an important transcription factor for osteoblast differentiation. However, bone morphogenetic protein (BMP)-2 treatment to stimulate Runx2 is not sufficient to acquire enough bone formation in osteoblasts. Therefore, it is necessary to find other regulatory factors which can improve the transcriptional activity of Runx2. The erythroblast transformation-specific (ETS) transcription factor family is reported to be involved in various aspects of cellular proliferation and differentiation. METHODS: We have noticed that the promoters of osteoblast differentiation markers such as alkaline phosphatase (Alp), osteopontin (Opn), and osteocalcin (Oc) contain Ets binding sequences which are also close to Runx2 binding elements. Luciferase assays were performed to measure the promoter activities of these osteoblast differentiation markers after the transfection of Runx2, myeloid Elf-1-like factor (MEF), and Runxs+MEF. Reverse-transcription polymerase chain reaction was also done to check the mRNA levels of Opn after Runx2 and MEF transfection into rat osteoblast (ROS) cells. RESULTS: We have found that MEF, an Ets transcription factor, increased the transcriptional activities of Alp, Opn, and Oc. The addition of Runx2 resulted in the 2- to 6-fold increase of the activities. This means that these two transcription factors have a synergistic effect on the osteoblast differentiation markers. Furthermore, early introduction of these two Runx2 and MEF factors significantly elevated the expression of the Opn mRNA levels in ROS cells. We also showed that Runx2 and MEF proteins physically interact with each other. CONCLUSIONS: Runx2 interacts with MEF proteins and binds to the promoters of the osteoblast markers such as Opn nearby MEF to increase its transcriptional activity. Our results also imply that osteoblast differentiation and bone formation can be increased by activating MEF to elicit the synergistic effect of Runx2 and MEF.
Alkaline Phosphatase ; Animals ; Antigens, Differentiation ; Bone and Bones ; Bone Morphogenetic Proteins ; Cell Differentiation ; Cell Proliferation ; Core Binding Factor Alpha 1 Subunit ; Erythroblasts ; Humans ; Luciferases ; Osteoblasts ; Osteocalcin ; Osteogenesis ; Osteopontin ; Polymerase Chain Reaction ; Proteins ; Rats ; RNA, Messenger ; Transcription Factors ; Transfection

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Micronutrients and superoxide dismutase in postmenopausal women with chronic periodontitis: a pilot interventional study.

Sunita DAIYA ; Rajinder Kumar SHARMA ; Shikha TEWARI ; Satish Chander NARULA ; Paramjeet KUMAR SEHGAL

Journal of Periodontal & Implant Science.2014;44(4):207-213. doi:10.5051/jpis.2014.44.4.207

PURPOSE: The study was aimed at investigating changes in periodontal parameters and superoxide dismutase activity triggered by root surface debridement with and without micronutrient supplementation in postmenopausal women. METHODS: Forty-three postmenopausal chronic periodontitis patients were divided into two groups: group 1 (n=22) were provided periodontal treatment in the form of scaling and root planing (SRP) and group 2 (n=21) patients received SRP along with systemic administration of micronutrient antioxidants. Patients in both groups were subjected to root surface debridement. Group 2 patients also received adjunctive micronutrient antioxidant supplementation. Serum and salivary superoxide dismutase (SOD) activity along with periodontal parameters were recorded at baseline and 3 months after therapy. RESULTS: Salivary and serum SOD values significantly (P<0.05) improved with periodontal treatment. Improvement in systemic enzymatic antioxidant status along with reduction in gingival inflammation and bleeding on probing (%) sites was significantly greater in group 2 as compared to group 1. CONCLUSIONS: Adjunctive micronutrient supplements reduce periodontal inflammation and improve the status of systemic enzymatic antioxidants in postmenopausal women.
Antioxidants ; Chronic Periodontitis* ; Debridement ; Female ; Hemorrhage ; Humans ; Inflammation ; Micronutrients* ; Root Planing ; Superoxide Dismutase*

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Outcomes of nonsurgical periodontal therapy in severe generalized aggressive periodontitis.

Amal BOUZIANE ; Latifa BENRACHADI ; Redouane ABOUQAL ; Oumkeltoum ENNIBI

Journal of Periodontal & Implant Science.2014;44(4):201-206. doi:10.5051/jpis.2014.44.4.201

PURPOSE: Aggressive periodontitis, especially in its severe form, was traditionally considered to have an unfavourable prognosis. It required a complex treatment and its stabilization was often achieved by surgical therapy. The aim of this study was to investigate the results of nonsurgical periodontal treatment in severe generalized forms of aggressive periodontitis. METHODS: Patients with advanced generalized aggressive periodontitis were included in the study. Probing depth (PD) of pockets > or =7 mm and clinical attachment level (CAL) of sites with attachment loss > or =5 mm were measured at baseline before nonsurgical periodontal treatment, at re-evaluation, and after treatment. The following other parameters were recorded: resolution of inflammation and bone fill. We compared the baseline values with re-evaluation and posttreatment values using the Friedman test. The Wilcoxon test with the Bonferroni correction was used for both re-evaluation and posttreatment values. RESULTS: Seven patients with 266 periodontal sites were examined. A significant difference was found between values, reported as medians with interquartile ranges, for PD at baseline (7.94 [7.33-8.19] mm) and both re-evaluation (4.33 [3.63-5.08] mm) and posttreatment (3.54 [3.33-4.11] mm) values (P=0.002). A significant difference was also found between values for CAL at baseline (9.02 [7.5-9.2] mm) and both re-evaluation (6.55 [6.30-6.87] mm) and posttreatment (6.45 [5.70-6.61] mm) (P=0.002). Inflammation was resolved and angular bone defects were repaired in all cases. CONCLUSIONS: These therapeutic results suggest that this form of periodontitis could have positive outcomes after nonsurgical periodontal treatment. The reparative potential of tissue affected by severe aggressive periodontitis should encourage clinicians to save apparently hopeless teeth in cases of this form of periodontitis.
Aggressive Periodontitis* ; Humans ; Inflammation ; Periodontal Debridement ; Periodontitis ; Prognosis ; Tooth ; Treatment Outcome

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Comparative study of two collagen membranes for guided tissue regeneration therapy in periodontal intrabony defects: a randomized clinical trial.

Young Mi CHUNG ; Jue Yeon LEE ; Seong Nyum JEONG

Journal of Periodontal & Implant Science.2014;44(4):194-200. doi:10.5051/jpis.2014.44.4.194

PURPOSE: The purpose of this study was to assess and compare the clinical and radiographic outcomes of guided tissue regeneration therapy for human periodontal intrabony defects using two different collagen membranes: a porous nonchemical cross-linking collagen membrane (NC) and a bilayer collagen membrane (BC). METHODS: Thirty subjects were randomly assigned and divided into the following 3 groups: a test group (NC+BM), in which a NC was used with xenograft bone mineral (BM), a positive control group (BC+BM), in which a BC was used with xenograft BM, and a negative control group (BM), in which only xenograft BM was used. The following clinical measurements were taken at baseline and 3 months after surgery: plaque index, gingival index, probing pocket depth, gingival recession, and clinical attachment level. Radiographic analysis was performed at baseline, 1 week and 3 months after surgery. RESULTS: Membrane exposure was not observed in any cases. Significant probing depth reduction, attachment-level gain and bone fill were observed for both test and control groups compared to baseline at 3 months after surgery (P<0.05). However, there were no statistically significant differences in clinical improvement and radiographic bone fill between treatment protocols (P>0.05). CONCLUSIONS: Within the limitations of this study, the results suggest that both NC and BC were comparable in terms of clinical and radiographic outcomes for the treatment of periodontal intrabony defects in human subjects.
Chronic Periodontitis ; Clinical Protocols ; Collagen* ; Gingival Recession ; Guided Tissue Regeneration* ; Heterografts ; Humans ; Membranes* ; Periodontal Index

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Accuracy of computer-aided template-guided oral implant placement: a prospective clinical study.

Mario BERETTA ; Pier Paolo POLI ; Carlo MAIORANA

Journal of Periodontal & Implant Science.2014;44(4):184-193. doi:10.5051/jpis.2014.44.4.184

PURPOSE: The aim of the present study was to evaluate the in vivo accuracy of flapless, computer-aided implant placement by comparing the three-dimensional (3D) position of planned and placed implants through an analysis of linear and angular deviations. METHODS: Implant position was virtually planned using 3D planning software based on the functional and aesthetic requirements of the final restorations. Computer-aided design/computer-assisted manufacture technology was used to transfer the virtual plan to the surgical environment. The 3D position of the planned and placed implants, in terms of the linear deviations of the implant head and apex and the angular deviations of the implant axis, was compared by overlapping the pre- and postoperative computed tomography scans using dedicated software. RESULTS: The comparison of 14 implants showed a mean linear deviation of the implant head of 0.56 mm (standard deviation [SD], 0.23), a mean linear deviation of the implant apex of 0.64 mm (SD, 0.29), and a mean angular deviation of the long axis of 2.42degrees (SD, 1.02). CONCLUSIONS: In the present study, computer-aided flapless implant surgery seemed to provide several advantages to the clinicians as compared to the standard procedure; however, linear and angular deviations are to be expected. Therefore, accurate presurgical planning taking into account anatomical limitations and prosthetic demands is mandatory to ensure a predictable treatment, without incurring possible intra- and postoperative complications.
Axis, Cervical Vertebra ; Computer-Aided Design ; Dental Implants ; Head ; Postoperative Complications ; Prospective Studies* ; Tomography, X-Ray Computed

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Case series of maxillary sinus augmentation with biphasic calcium phosphate: a clinical and radiographic study.

Jae Kook CHA ; Jung Chul PARK ; Ui Won JUNG ; Chang Sung KIM ; Kyoo Sung CHO ; Seong Ho CHOI

Journal of Periodontal & Implant Science.2011;41(2):98-104. doi:10.5051/jpis.2011.41.2.98

PURPOSE: The aim of this study was to evaluate 3.5 years-cumulative survival rate of implants placed on augmented sinus using Osteon, a bone graft material, and to assess the height of the grafted material through radiographic evaluation. METHODS: Twenty patients were treated with maxillary sinus augmentation and 45 implant fixtures were installed simultaneously or after 6 months healing period. The height of the augmented sinus and the loss of marginal bone were measured by panoramic and intraoral radiographs immediately after augmentation and up to 42 months (mean, 19.4 months) subsequently. Changes in the height of the sinus graft material were calculated radiographically. RESULTS: The cumulative survival rate was 95.56% in all 45 implants. Additionally, normal healing process without any complication was observed in all patients. The original sinus height was mean 4.3 mm and the augmented sinus height was mean 13.4 mm after the surgery. The mean marginal bone loss till 42 months was 0.52+/-0.56 mm. The reduced height of Osteon was 0.83+/-0.38 mm and it did not show significant correlation with the follow up periods (P=0.102). There were no statistically significant differences in reduced height of Osteon according to the simultaneous/delayed implantation (P=0.299) and particle size of Osteon (P=0.644). CONCLUSIONS: It can be suggested that Osteon may have predictable result when it was used as a grafting material for sinus floor augmentation.
Calcium ; Dental Implants ; Follow-Up Studies ; Haversian System ; Humans ; Maxillary Sinus ; Particle Size ; Sinus Floor Augmentation ; Survival Rate ; Transplants

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Comparative study on the results of non-surgical periodontal treatment according to the location of the affected site.

Ju Min LEE ; Joo Hee KIM ; Eun Young KWON ; Yi Kyeong KIM ; Ju Yeon LEE ; Sung Jo KIM ; Jeom Il CHOI

Journal of Periodontal & Implant Science.2011;41(2):92-97. doi:10.5051/jpis.2011.41.2.92

PURPOSE: The present study was performed to compare the treatment outcomes of non-surgical periodontal treatment according to the distribution of attachment loss of a given patient. METHODS: Forty-five patients with moderate to severe periodontitis were divided in two subgroups; Group I patients with teeth manifesting attachment loss of > or =6 mm at one or more sites on the buccal/labial aspect while maintaining an attachment level < or =5 mm at the lingual/palatal aspect, Group II patients with teeth manifesting an attachment level > or =6 mm at more than one site on the lingual/palatal aspect while maintaining an attachment level < or =5 mm at the buccal/labial aspect. The probing pocket depth, probing attachment level, tooth mobility, and chewing discomfort were recorded at baseline and 6 months examinations following non-surgical periodontal therapy. RESULTS: The buccal/labial surfaces of teeth with moderate to severe periodontitis in Group I patients demonstrated a greater amount of pocket reduction, gain of attachment level, and tooth mobility reduction than the lingual/palatal aspects of teeth examined in Group II patients. CONCLUSIONS: Within the limits of the present study, the patients demonstrating attachment loss > or =6 mm at buccal/labial surfaces responded better to the nonsurgical periodontal therapy than those demonstrating comparable attachment loss at lingual/palatal surfaces.
Humans ; Mastication ; Periodontal Pocket ; Periodontitis ; Prognosis ; Tooth ; Tooth Mobility

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Cumulative survival rate of Astra Tech implants: a retrospective analysis.

Jung Soo KIM ; Joo Yeon SOHN ; Jung Chul PARK ; Ui Won JUNG ; Chang Sung KIM ; Jae Hoon LEE ; June Sung SHIM ; Keun Woo LEE ; Seong Ho CHOI

Journal of Periodontal & Implant Science.2011;41(2):86-91. doi:10.5051/jpis.2011.41.2.86

PURPOSE: The aim of this study was to analyze the short-term predictability and reliability of Astra Tech implants according to the demographical distribution of patients and condition of implant sites and location of implants. METHODS: Among patients treated with Astra Tech implant (Astra Tech AB) in the Department of Periodontology at the Dental Hospital of Yonsei University of College of Dentisry and K Dental Clinic from May 2004 to March 2009, 195 implants in 98 patients which had been restored more than 6 months ago were reviewed in this study. Following data were reviewed from patient charts and implants success rate was examined: 1) patient type and implant location, 2) bone status at the implant site, 3) diameter and length of the placed implants, 4) presence or absence of bone augmentation and types of the augmentation. RESULTS: The results from this study are as follows: 1) most implants were placed in the molar area, especially 1st molar area of maxilla, 2) most implants were placed at D2 and D3 bone type, 3) most implants were placed in areas of B and C bone quantity, 4) autogenous and alloplastic bone graft and artificial membrane were used for placement of 74 implants. CONCLUSIONS: Short-term survival rate of Astra Tech implants was 100%.
Dental Clinics ; Dental Implantation ; Humans ; Maxilla ; Membranes, Artificial ; Molar ; Retrospective Studies ; Survival Rate ; Transplants

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Masticatory function following implants replacing a second molar.

Moon Sun KIM ; Jae Kwan LEE ; Beom Seok CHANG ; Heung Sik UM

Journal of Periodontal & Implant Science.2011;41(2):79-85. doi:10.5051/jpis.2011.41.2.79

PURPOSE: The aim of this study was to obtain objective and standardized information on masticatory function and patient satisfaction following second molar single implant therapy. METHODS: Twenty adult patients, who had restored second molar single implants more than 1 month before the study, were enrolled in this study. All patients received a chewing test using peanuts before and after insertion of the implant prosthesis, with a questionnaire and visual analogue scale (VAS) to evaluate the effect of second molar single implant therapy. RESULTS: This study obtained standardized information on the masticatory function objectively (e.g., P, R, X50) before (Pre-insertion) and after insertion (Post-insertion) of the implant prosthesis. Masticatory performance (P) after insertion of the implant prosthesis significantly increased from 67.8+/-9.9 to 84.3+/-8.5% (P<0.0001). With the implant prosthesis, the P value increased by 24%. The masticatory efficiency index (R) of Post-insertion is higher than that of Pre-insertion (P<0.0001). With the implant prosthesis, the R value increased by 29%. The median particle size (X50) of Post-insertion is lower than that of Pre-insertion (P<0.0001). More than 90% of the patients were satisfied with the second molar single implant therapy from a functional point of view. CONCLUSIONS: These findings indicate that a second molar single implant can increase masticatory function.
Adult ; Arachis ; Dental Implants, Single-Tooth ; Humans ; Mastication ; Molar ; Particle Size ; Patient Satisfaction ; Prostheses and Implants ; Surveys and Questionnaires

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Effect of seeding using an avidin-biotin binding system on the attachment of periodontal ligament fibroblasts to nanohydroxyapatite scaffolds: three-dimensional culture.

Yong Ju JANG ; Im Hee JUNG ; Jung Chul PARK ; Ui Won JUNG ; Chang Sung KIM ; Yong Keun LEE ; Chong Kwan KIM ; Seong Ho CHOI

Journal of Periodontal & Implant Science.2011;41(2):73-78. doi:10.5051/jpis.2011.41.2.73

PURPOSE: For periodontal tissue engineering, it is a primary requisite and a challenge to select the optimum types of cells, properties of scaffold, and growth factor combination to reconstruct a specific tissue in its natural form and with the appropriate function. Owing to fundamental disadvantages associated with using a two-dimensional substrate, several methods of seeding cells into three-dimensional scaffolds have been reported and the authors have asserted its usefulness and effectiveness. In this study, we explore the cell attachment of periodontal ligament fibroblasts on nanohydroxyapatite (n-HA) scaffold using avidin biotin binding system (ABBS). METHODS: Human periodontal ligament fibroblasts were isolated from the health tooth extracted for the purpose of orthodontic procedure. HA nanoparticles were prepared and Ca(NO3)2-4H2O and (OC2H5)3P were selected as precursors of HA sol. The final scaffold was 8 mm in diameter and 3 mm in height disk with porosity value of 81.55%. 1x10(5) periodontal ligament fibroblasts were applied to each scaffold. The cells were seeded into scaffolds by static, agitating and ABBS seeding method. RESULTS: The number of periodontal ligament fibroblasts attached was greater for ABBS seeding method than for static or agitating method (P<0.05). No meaningful difference has been observed among seeding methods with scanning electron microscopy images. However, increased strength of cell attachment of ABBS could be deduced from the high affinity between avidin and biotin (Kd=10(-15) M). CONCLUSIONS: The high-affinity ABBS enhances the ability of periodontal ligament fibroblasts to attach to three-dimensionally constructed n-HA scaffolds.
Avidin ; Biotin ; Cell Adhesion ; Dihydroergotamine ; Fibroblasts ; Humans ; Microscopy, Electron, Scanning ; Nanoparticles ; Periodontal Ligament ; Polymethyl Methacrylate ; Porosity ; Seeds ; Tissue Engineering ; Tooth

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