Book Review: Writing Science: How to Write Papers That Get Cited and Proposals That Get Funded.
International Neurourology Journal.2014;18(2):101-101.
International Neurourology Journal
to Present ISSN: 2093-4777
Sort by
DISPLAY OPTIONS
Format:
Per page:
Dexmedetomidine Oral Mucosa Patch for Sedation Suppresses Apoptosis in Hippocampus of Normal Rats.
International Neurourology Journal.2017;21(Suppl 1):S39-S47. doi:10.5213/inj.1734884.442
PURPOSE: Dexmedetomidine, an α2-adrenergic agonist, provides sedative and analgesic effects without significant respiratory depression. Dexmedetomidine has been suggested to have an antiapoptotic effect in response to various brain insults. We developed an oral mucosa patch using dexmedetomidine for sedation. The effects of the dexmedetomidine oral mucosa patch on cell proliferation and apoptosis in the hippocampus were evaluated. METHODS: A hydrogel oral mucosa patch was adhered onto the oral cavity of physiologically normal rats, and was attached for 2 hours, 6 hours, 12 hours, or 24 hours. Plasma dexmedetomidine concentrations were determined by liquid chromatography– electrospray ionization–tandem mass spectrometry–multiple-ion reaction monitoring (LC-ESI-MS/MS-MRM). Cell proliferation in the hippocampus was detected by Ki-67 immunohistochemistry. Caspase-3 immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining, and Western blotting for Bax and Bcl-2 were performed to detect hippocampal apoptosis. The levels of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) in the hippocampus were also measured by Western blotting. RESULTS: Plasma dexmedetomidine concentration increased according to the attachment time of the dexmedetomidine oral mucosa patch. Hippocampal cell proliferation did not change due to the dexmedetomidine oral mucosa patch, and the dexmedetomidine oral mucosa patch exerted no significant effect on BDNF or TrkB expression. In contrast, the dexmedetomidine oral mucosa patch exerted an antiapoptotic effect depending on the attachment time of the dexmedetomidine oral mucosa patch. CONCLUSIONS: A dexmedetomidine oral mucosa patch can be used as a convenient tool for sedation, and is of therapeutic value due to its antiapoptotic effects under normal conditions.
Animals ; Apoptosis* ; Blotting, Western ; Brain ; Brain-Derived Neurotrophic Factor ; Caspase 3 ; Cell Proliferation ; Dexmedetomidine* ; Hippocampus* ; Hydrogel ; Immunohistochemistry ; Mouth ; Mouth Mucosa* ; Plasma ; Protein-Tyrosine Kinases ; Rats* ; Respiratory Insufficiency
Animals ; Apoptosis* ; Blotting, Western ; Brain ; Brain-Derived Neurotrophic Factor ; Caspase 3 ; Cell Proliferation ; Dexmedetomidine* ; Hippocampus* ; Hydrogel ; Immunohistochemistry ; Mouth ; Mouth Mucosa* ; Plasma ; Protein-Tyrosine Kinases ; Rats* ; Respiratory Insufficiency
International Neurourology Journal.2017;21(Suppl 1):S32-S38. doi:10.5213/inj.1734878.439
PURPOSE: To realistically map the electric fields of biological tissues using a diffusion tensor magnetic resonance electrical impedance tomography (DT-MREIT) method to estimate tissue response during electrical stimulation. METHODS: Imaging experiments were performed using chunks of bovine muscle. Two silver wire electrodes were positioned inside the muscle tissue for electrical stimulation. Electric pulses were applied with a 100-V amplitude and 100-μs width using a voltage stimulator. During electrical stimulation, we collected DT-MREIT data from a 3T magnetic resonance imaging scanner. We adopted the projected current density method to calculate the electric field. Based on the relation between the water diffusion tensor and the conductivity tensor, we computed the position-dependent scale factor using the measured magnetic flux density data. Then, a final conductivity tensor map was reconstructed using the multiplication of the water diffusion tensor and the scale factor. RESULTS: The current density images from DT-MREIT data represent the internal current flows that exist not only in the electrodes but also in surrounding regions. The reconstructed electric filed map from our anisotropic conductivity tensor with the projected current density shows coverage that is more than 2 times as wide, and higher signals in both the electrodes and surrounding tissues, than the previous isotropic method owing to the consideration of tissue anisotropy. CONCLUSIONS: An electric field map obtained by an anisotropic reconstruction method showed different patterns from the results of the previous isotropic reconstruction method. Since accurate electric field mapping is important to correctly estimate the coverage of the electrical treatment, future studies should include more rigorous validations of the new method through in vivo and in situ experiments.
Anisotropy ; Diffusion Tensor Imaging ; Diffusion* ; Electric Conductivity* ; Electric Impedance ; Electric Stimulation* ; Electrodes ; Magnetic Resonance Imaging ; Methods ; Silver ; Water*
Anisotropy ; Diffusion Tensor Imaging ; Diffusion* ; Electric Conductivity* ; Electric Impedance ; Electric Stimulation* ; Electrodes ; Magnetic Resonance Imaging ; Methods ; Silver ; Water*
International Neurourology Journal.2017;21(Suppl 1):S24-S31. doi:10.5213/inj.1734856.428
PURPOSE: The differentiation properties of stem cells are not yet fully understood due to their close association with multiple environmental and extrinsic factors. This study investigates the differentiation properties of mesenchymal stem cells (MSCs) and correlates them with their intrinsic mechanical properties. METHODS: A total of 3 different types of MSCs, namely bone marrow-derived MSCs (BMSCs), umbilical cord-derived MSCs (UCSCs), and adipose-derived MSCs (ADSCs) were evaluated. These 3 MSCs were individually differentiated into adipocytes and osteoblasts for 3 weeks. The mechanical properties of the MSCs and differentiated cells were determined by atomic force microscopy. RESULTS: ADSCs showed the greatest ability to differentiate into adipocytes, followed by BMSCs and UCSCs. While UCSCs differentiated readily into osteoblasts, BMSCs and ADSCs were less likely to undergo this differentiation. UCSCs were the “hardest” cells, while ADSCs were the “softest.” The cells differentiated from “hard” MSCs were stiffer than the cells differentiated from “soft” MSCs, irrespective of lineage specification. CONCLUSIONS: The differentiation ability of MSCs and the mechanical properties of the differentiated cells were closely linked. However, there were no significant correlations regarding changes in the mechanical properties between the nuclear region and the cytoplasm during differentiation.
Adipocytes ; Adipogenesis ; Cytoplasm ; Mechanics ; Mesenchymal Stromal Cells* ; Microscopy, Atomic Force ; Osteoblasts ; Osteogenesis ; Stem Cells
Adipocytes ; Adipogenesis ; Cytoplasm ; Mechanics ; Mesenchymal Stromal Cells* ; Microscopy, Atomic Force ; Osteoblasts ; Osteogenesis ; Stem Cells
International Neurourology Journal.2017;21(Suppl 1):S17-S23. doi:10.5213/inj.1734858.429
PURPOSE: The aim of this study was to perform a histological analysis of the effect of a ytterbium-doped fiber (YDF) laser on oral buccal mucosa tissue in vivo to simulate its effect on the mucosa of the lower urinary tract. METHODS: A total of 90 8-week-old Sprague-Dawley rats were anesthetized with urethrane (1.2 g/kg intraperitoneally). A pre-specified inner buccal mucosal site was irradiated with a YDF master-oscillator power amplifier (MOPA) system for 60 seconds, with output power settings of 0.5, 1, and 2 W, respectively, in 3 treatment groups. Specimens of irradiated tissue were harvested at 2 hours, 24 hours, 2 weeks, and 4 weeks after irradiation. The tissue specimens were stained with hematoxylin and eosin for histological analysis. RESULTS: In the group treated with 0.5 W, basal cell elongation and vacuolization were observed at 2 hours and 24 hours after treatment, respectively. No evident injury was observed after 2 or 4 weeks. The group treated with 1 W presented partial basal layer separation, and even complete epidermal ablation, within 2 hours. At 24 hours after laser treatment, new capillaries on an edematous background of fibroblasts and myofibroblasts, as well as profuse infiltration of the neutrophils to the basal layer, were observed. Collagen deposition and reepithelization were observed in specimens taken 2 weeks and 4 weeks after treatment. The group treated with 2 W presented bigger and deeper injuries at 2 hours after irradiation. Meanwhile, subepidermal bullae with full-thickness epidermal necrosis and underlying inflammatory infiltrate were observed 24 hours after treatment. The presence of fibrous connective tissue and collagen deposition were observed 2 weeks and 4 weeks after the treatment. CONCLUSIONS: To our knowledge, this is the first report regarding the effect of a YDF laser on living tissue. Our study demonstrated that the typical histological findings of the tissue reaction to the YDF MOPA apparatus were very similar to those associated with thermal injuries. The extent and degree of tissue damage increased proportionally to the output power.
Animals ; Capillaries ; Collagen ; Connective Tissue ; Eosine Yellowish-(YS) ; Fibroblasts ; Hematoxylin ; Mouth Mucosa* ; Mucous Membrane ; Myofibroblasts ; Necrosis ; Neutrophils ; Prostatic Hyperplasia ; Rats* ; Rats, Sprague-Dawley ; Urinary Tract* ; Wound Healing
Animals ; Capillaries ; Collagen ; Connective Tissue ; Eosine Yellowish-(YS) ; Fibroblasts ; Hematoxylin ; Mouth Mucosa* ; Mucous Membrane ; Myofibroblasts ; Necrosis ; Neutrophils ; Prostatic Hyperplasia ; Rats* ; Rats, Sprague-Dawley ; Urinary Tract* ; Wound Healing
Noninvasive Medical Tools for Evaluating Voiding Pattern in Real Life.
International Neurourology Journal.2017;21(Suppl 1):S10-S16. doi:10.5213/inj.1734860.430
Voiding dysfunction is a common disease that contributes to a lower quality of life and has an increased prevalence in the elderly population. Noninvasive and objective methods such as uroflowmetry (UFM) and voiding diaries (VDs) are essential for exact diagnosis and effective treatment of this condition because patients with different causes of voiding dysfunction can complain of the same lower urinary tract symptoms. Further, different treatment options can be determined based on the diagnosis made from these symptoms. In order to improve the quality of UFM and VDs and to provide a convenient testing environment, several advances have been made by previous investigators. In this study, we investigate the history and technological mechanisms of UFM and VDs. We also aim to review UFM from the viewpoint of clinical and at-home uses, including the recently proposed toilet-shaped UFM and electronic VDs.
Aged ; Diagnosis ; Humans ; Lower Urinary Tract Symptoms ; Mobile Health Units ; Monitoring, Ambulatory ; Prevalence ; Quality of Life ; Research Personnel
Aged ; Diagnosis ; Humans ; Lower Urinary Tract Symptoms ; Mobile Health Units ; Monitoring, Ambulatory ; Prevalence ; Quality of Life ; Research Personnel
Applications of Microfluidic Devices for Urology.
International Neurourology Journal.2017;21(Suppl 1):S4-S9. doi:10.5213/inj.1734838.419
Microfluidics is considered an important technology that is suitable for numerous biomedical applications, including cancer diagnosis, metastasis, drug delivery, and tissue engineering. Although microfluidics is still considered to be a new approach in urological research, several pioneering studies have been reported in recent years. In this paper, we reviewed urological research works using microfluidic devices. Microfluidic devices were used for the detection of prostate and bladder cancer and the characterization of cancer microenvironments. The potential applications of microfluidics in urinary analysis and sperm sorting were demonstrated. The use of microfluidic devices in urology research can provide high-throughput, high-precision, and low-cost analyzing platforms.
Diagnosis ; Lab-On-A-Chip Devices* ; Microfluidics* ; Neoplasm Metastasis ; Prostate ; Prostatic Neoplasms ; Spermatozoa ; Tissue Engineering ; Tumor Microenvironment ; Urinary Bladder Neoplasms ; Urology*
Diagnosis ; Lab-On-A-Chip Devices* ; Microfluidics* ; Neoplasm Metastasis ; Prostate ; Prostatic Neoplasms ; Spermatozoa ; Tissue Engineering ; Tumor Microenvironment ; Urinary Bladder Neoplasms ; Urology*
New Equipment and Devices for Therapeutic Purpose.
International Neurourology Journal.2017;21(Suppl 1):S2-S3. doi:10.5213/inj.1720edi004
No abstract available.
Collaborative Efforts Among the Fields of Technology, Device Development, and Medicine.
International Neurourology Journal.2017;21(Suppl 1):S1-S1. doi:10.5213/inj.1720edi003
International Neurourology Journal.2014;18(1):48-48.
No abstract available.
Pelvic Pain*
Pelvic Pain*
Country
Republic of Korea
Publisher
Korean Continence Society
ElectronicLinks
http://einj.org/Editor-in-chief
Tack Lee
moonuro@hanyang.ac.kr
Abbreviation
Int Neurourol J
Vernacular Journal Title
대한배뇨장애요실금학회지
ISSN
2093-4777
EISSN
2093-6931
Year Approved
2007
Current Indexing Status
Currently Indexed
Start Year
Description
The official journal of the Korean Continence Society, Korean Society of Urological Research, Korean Children's Continence and Enuresis Society, and the Korean Association of Urogenital Tract Infection and Inflammation. The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that describe the most significant and promising achievements in all fields of science and technology, as well as clinical neurourology.
Previous Title
Powered by IMICAMS(
备案号: 11010502037788, 京ICP备10218182号-8)