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Size analysis and the reference area of acetabulum-prosthesis-transplantation in different body positions. .

Hui ZENG ; Eckart MAYR ; Bin KANG ; Ao XIONG ; Feng XIN ; Michael NOGLER ; Martin KRISMER

Journal of Huazhong University of Science and Technology (Medical Sciences).2006;26(3):322-324.

Acetabulum ; transplantation ; Adult ; Aged ; Aged, 80 and over ; Arthroplasty, Replacement, Hip ; methods ; Female ; Hip Joint ; surgery ; Humans ; Male ; Middle Aged

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beta-catenin/Tcf signaling in squamous differentiation of porcine airway epithelial cells.

Wenshu CHEN ; Renliang WU ; Xi WANG

Journal of Huazhong University of Science and Technology (Medical Sciences).2008;28(2):121-124. doi:10.1007/s11596-008-0201-y

For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
Animals ; Cell Differentiation ; Cell Nucleus ; metabolism ; Cells, Cultured ; Cornified Envelope Proline-Rich Proteins ; Cytoplasm ; metabolism ; Epithelial Cells ; cytology ; metabolism ; Membrane Proteins ; metabolism ; Mutation ; RNA, Messenger ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Swine ; Trachea ; cytology ; metabolism ; Transcription, Genetic ; beta Catenin ; metabolism

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Splenectomy suppresses growth and metastasis of hepatocellular carcinoma through decreasing myeloid-derived suppressor cells in vivo.

Xin LONG ; Jian WANG ; Jian-Ping ZHAO ; Hui-Fang LIANG ; Peng ZHU ; Qi CHENG ; Qian CHEN ; Yan-Hui WU ; Zhan-Guo ZHANG ; Bi-Xiang ZHANG ; Xiao-Ping CHEN

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(5):667-676.

The function of the spleen in tumor development has been investigated for years. The relationship of the spleen with hepatocellular carcinoma (HCC), a huge health burden worldwide, however, remains unknown. The present study aimed to examine the effect of splenectomy on the development of HCC and the possible mechanism. Mouse hepatic carcinoma lines H22 and Hepa1-6 as well as BALB/c and C57 mice were used to establish orthotopic and metastatic mouse models of liver cancer. Mice were divided into four groups, including control group, splenectomy control group (S group), tumor group (T group) and tumor plus splenectomy group (T+S group). Tumor growth, metastases and overall survival were assessed at determined time points. Meanwhile, myeloid-derived suppressor cells (MDSCs) were isolated from the peripheral blood (PB), the spleen and liver tumors, and then measured by flow cytometery. It was found that liver cancer led to splenomegaly, and increased the percentage of MDSCs in the PB and spleen in the mouse models. Splenectomy inhibited the growth and progression of liver cancer and prolonged the overall survival time of orthotopic and metastatic models, which was accompanied by decreased proportion of MDSCs in the PB and tumors of liver cancer-bearing mouse. It was suggested that splenectomy could be considered an adjuvant therapy to treat liver cancer.
Animals ; Carcinoma, Hepatocellular ; physiopathology ; surgery ; Cell Line, Tumor ; Flow Cytometry ; Humans ; Liver Neoplasms ; physiopathology ; surgery ; Mice ; Myeloid-Derived Suppressor Cells ; pathology ; Neoplasms, Experimental ; physiopathology ; surgery ; Spleen ; physiopathology ; surgery ; Splenectomy ; methods

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QTc interval predicts outcome of catheter ablation in paroxysmal atrial fibrillation patients with type 2 diabetes mellitus.

Ning MA ; Xiao-Yan WU ; Chang-Sheng MA ; Nian LIU ; Rong BAI ; Xin DU ; Yan-Fei RUAN ; Jian-Zeng DONG

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(5):646-652.

Catheter ablation has been recommended as a treatment option for paroxysmal atrial fibrillation (PAF) patients complicated with type 2 diabetes mellitus (T2DM). PAF patients with T2DM have a higher recurrence rate after catheter ablation. Prolongation of corrected QT (QTc) interval has been linked to poor outcomes in T2DM patients. Whether the abnormal QTc interval is associated with the ablation outcome in the PAF patients with T2DM remains unknown. In this study, 134 PAF patients with T2DM undergoing primary catheter ablation were retrospectively enrolled. Pre-procedural QTc interval was corrected by using the Bazett's formula. Cox proportional hazards models were constructed to assess the relationship between QTc interval and the recurrence of AF. After a 29.1-month follow-up period, 61 patients experienced atrial tachyarrhythmia recurrence. Recurrent patients had a longer QTc interval than non-recurrent patients (425.2±21.5 ms vs. 414.1±13.4 ms, P=0.002). Multivariate Cox regression analysis revealed that QTc interval [hazard ratio (HR)=1.026, 95% confidence interval (CI) 1.012-1.040, P=0.005] and left atrial diameter (LAD) (HR=1.125, 95% CI 1.062-1.192, P=0.003) were independent predictors of recurrent atrial tachyarrhythmia. Receiver operating characteristic analysis demonstrated that the cut-off value of QTc (418 ms) predicted arrhythmia recurrence with a sensitivity of 55.7% and a specificity of 69.9%. A combination of LAD and QTc was more effective than LAD alone (P<0.001) in predicting arrhythmia recurrence after the procedure. QTc interval could be used as an independent predictor of arrhythmia recurrence in T2DM patients undergoing AF ablation, thus providing a simple method to identify those patients who likely have a better outcome following the procedure.
Aged ; Atrial Fibrillation ; complications ; physiopathology ; surgery ; Catheter Ablation ; adverse effects ; Diabetes Mellitus, Type 2 ; complications ; physiopathology ; surgery ; Electrocardiography ; Female ; Heart Atria ; physiopathology ; Humans ; Male ; Middle Aged ; Proportional Hazards Models ; Risk Factors ; Tachycardia ; physiopathology ; Treatment Outcome

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Inhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis.

Shuang CHEN ; Jie YAN ; Hai-Xiao DENG ; Ling-Ling LONG ; Yong-Jun HU ; Mi WANG ; Lei SHANG ; Dan CHEN ; Ju-Fang HUANG ; Kun XIONG

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(5):639-645.

The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco's-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
Animals ; Apoptosis Inducing Factor ; biosynthesis ; genetics ; Calpain ; biosynthesis ; genetics ; Gene Expression Regulation ; drug effects ; Glucose ; metabolism ; Humans ; Leupeptins ; administration & dosage ; Mice ; Oxygen ; metabolism ; Retinal Ganglion Cells ; metabolism ; pathology ; Retinal Necrosis Syndrome, Acute ; genetics ; pathology

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Erratum to: Human brucellosis mimicking axial spondyloarthritis: a challenge for rheumatologists when applying the 2009 ASAS criteria.

Cong YE ; Gui-Fen SHEN ; Shou-Xin LI ; Ling-Li DONG ; Yi-Kai YU ; Wei TU ; Ying-Zi ZHU ; Shao-Xian HU

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(4):618-618.

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Establishment of reference intervals and transfusion criterion for Sonoclot analysis.

Zhen-Lu ZHANG ; You-Ping CHEN ; Cui-Hua TAO ; Xiao-Hui LIU ; Meng-Ya LI ; Xin ZHOU

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(4):614-617. doi:10.1007/s11596-016-1634-3

Sonoclot analyzer has been widely used in many countries. But the reference intervals provided by the manufacturer were derived from only 45 participants, and there was no cut-off value for transfusion for Sonoclot analysis. This study aimed to establish reference intervals and transfusion criterion for Sonoclot analysis. Volunteers were recruited from healthy Chinese adults and patients undergoing cardiac surgery. Blood samples were withdrawn from forearm vein and measured for activated clotting time (ACT), clot rate (CR), platelet function (PF), activated partial thromboplastin time (APTT), fibrinogen concentration (FIB), and platelet count (PLT). The reference intervals were determined by the nonparametric method. Cut-off values were determined by the receiver operating characteristics curve. A total of 135 healthy volunteers and 281 patients were enrolled. The 95% reference intervals were 96-195 s, 22-51 signal U/min, >1.6 for ACT, CR, PF respectively. In the 281 patients, the results of APTT, FIB, PLT, ACT, CR, and PF ranged from 20.5-300.0 s, 0.28-4.11 g/L, (19.0-387.3)×109/L, 80-514 s, 2.9-74 signal U/min, and 0.1-5.1 respectively. The cut-off values for transfusion were >208, ≤14, and ≤1.3 for ACT, CR, PF respectively. The cut-off values of Sonoclot analysis were within the manufacturer's reference intervals, while they were outside the reference intervals established in this study. The results suggested that the manufacturer's reference intervals were not suitable for Chinese. The reference intervals and cut-off values established in this study will be helpful to Chinese patients.
Adolescent ; Adult ; Aged ; Blood Coagulation ; Cardiopulmonary Bypass ; China ; Female ; Fibrinogen ; metabolism ; Humans ; Male ; Middle Aged ; Partial Thromboplastin Time ; methods ; Platelet Count ; Point-of-Care Systems ; Reference Values

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Bioinformatic analysis of non-VP1 capsid protein of coxsackievirus A6.

Hong-Bo LIU ; Guang-Fei YANG ; Si-Jia LIANG ; Jun LIN

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(4):607-613. doi:10.1007/s11596-016-1633-4

This study bioinformatically analyzed the non-VP1 capsid proteins (VP2-VP4) of Coxasckievirus A6 (CVA6), with an attempt to predict their basic physicochemical properties, structural/functional features and linear B cell eiptopes. The online tools SubLoc, TargetP and the others from ExPASy Bioinformatics Resource Portal, and SWISS-MODEL (an online protein structure modeling server), were utilized to analyze the amino acid (AA) sequences of VP2-VP4 proteins of CVA6. Our results showed that the VP proteins of CVA6 were all of hydrophilic nature, contained phosphorylation and glycosylation sites and harbored no signal peptide sequences and acetylation sites. Except VP3, the other proteins did not have transmembrane helix structure and nuclear localization signal sequences. Random coils were the major conformation of the secondary structure of the capsid proteins. Analysis of the linear B cell epitopes by employing Bepipred showed that the average antigenic indices (AI) of individual VP proteins were all greater than 0 and the average AI of VP4 was substantially higher than that of VP2 and VP3. The VP proteins all contained a number of potential B cell epitopes and some eiptopes were located at the internal side of the viral capsid or were buried. We successfully predicted the fundamental physicochemical properties, structural/functional features and the linear B cell eiptopes and found that different VP proteins share some common features and each has its unique attributes. These findings will help us understand the pathogenicity of CVA6 and develop related vaccines and immunodiagnostic reagents.
Amino Acid Sequence ; Capsid Proteins ; genetics ; immunology ; Computational Biology ; Enterovirus ; genetics ; pathogenicity ; Epitopes, B-Lymphocyte ; genetics ; immunology ; Humans

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Tuberous sclerosis complex: Imaging characteristics in 11 cases and review of the literature.

Shan HU ; Dao-Yu HU ; Wen-Zhen ZHU ; Liang WANG ; Zi WANG

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(4):601-606. doi:10.1007/s11596-016-1632-5

Tuberous sclerosis complex (TSC) is an uncommon multiorgan disorder that may present many and different manifestations on imaging. Radiology plays an important role in diagnosis and management, and can substantially improve the clinical outcome of TSC. Therefore, a comprehensive understanding of this disease is essential for the radiologist. The manifestations of TSC on computer tomography (CT) and magnetic resonance (MR) images were analyzed. Eleven patients with a clinical diagnosis of TSC were retrospectively reviewed. Central nervous system lesions included subependymal nodules (SENs) (11/11), subependymal giant cell astrocytomas (SEGAs) (2/11), cortical and subcortical tuber lesions (5/11), and white matter lesions (4/11). Of the 6 patients with abdominal scans, there were 6 cases of renal angiomyolipomas (AMLs), and one case of hepatic AMLs. Of the 4 patients undergoing chest CT, lung lymhangioleiomyomatosis (LAM) (2/4), and multiple small sclerotic bone lesions (2/4) were observed. Different modalities show different sensitivity to the lesion. Analysis of images should be integrated with patients' history in order to diagnose TSC.
Adolescent ; Adult ; Brain ; diagnostic imaging ; pathology ; Child ; Child, Preschool ; Female ; Glioma, Subependymal ; diagnosis ; diagnostic imaging ; pathology ; Humans ; Lung ; diagnostic imaging ; pathology ; Magnetic Resonance Imaging ; methods ; Male ; Middle Aged ; Nervous System Diseases ; diagnosis ; diagnostic imaging ; pathology ; Tuberous Sclerosis ; classification ; diagnosis ; diagnostic imaging ; pathology

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Discrimination of metastatic from non-metastatic mesorectal lymph nodes in rectal cancer using quantitative dynamic contrast-enhanced magnetic resonance imaging.

Xiao-Ping YU ; Lu WEN ; Jing HOU ; Hui WANG ; Qiang LU

Journal of Huazhong University of Science and Technology (Medical Sciences).2016;36(4):594-600. doi:10.1007/s11596-016-1631-6

Preoperative detection of lymph nodes (LNs) metastasis is always highly challenging for radiologists nowadays. The utility of quantitative dynamic contrast-enhanced magnetic resonance imaging (QDCE-MRI) in identifying LNs metastasis is not well understood. In the present study, 59 patients with histologically proven rectal carcinoma underwent preoperative QDCE-MRI. The short axis diameter ratio, long axis diameter ratio, short-to-long axis diameter ratio and QDEC-MRI parameters (K(trans), Kep, fPV and Ve) values were compared between the non-metastatic (n=44) and metastatic (n=35) LNs groups based on pathological examination. Compared with the non-metastatic group, the metastatic group exhibited significantly higher short axis diameter (7.558±0.668 mm vs. 5.427±0.285 mm), K(trans) (0.483±0.198 min(-1) vs. 0.218±0.116 min(-1)) and Ve (0.399±0.118 vs. 0.203±0.096) values (all P<0.05). The short-to-long axis diameter ratio, long axis diameter ratio, Kep and fPV values did not show significant differences between the two groups. In conclusion, our results showed that for LNs larger than 5 mm in rectal cancer, there are distinctive differences in the K(trans) and Ve values between the metastatic and non-metastatic LNs, suggesting that QDCE-MRI may be potentially helpful in identifying LNs status.
Contrast Media ; therapeutic use ; Diagnosis, Differential ; Female ; Humans ; Lymph Nodes ; diagnostic imaging ; pathology ; Lymphatic Metastasis ; diagnosis ; diagnostic imaging ; pathology ; Magnetic Resonance Imaging ; Male ; Rectal Neoplasms ; diagnosis ; diagnostic imaging ; pathology

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