Journal Detail Information

1

Cite

Share

Evaluation on clinical application of three testing methods for human cytomegalovirus infection in pregnancy.

Wanjiang ZENG ; Liangzhen WEN ; Suhua CHEN ; Xiazhen LING

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):192-194.

The value of ELISA, N-PCR and RT-PCR in clinical practice for pregnant women with HCMV infection was investigated. 5581 pregnant women were screened by ELISA. Among them, 100 cases were positive for IgM (group 1), 69 for both IgM and serous DNA (group 2) and 69 for both IgM and mRNA (group 3). The infectious status, maternal-fetal transmission and pregnancy outcome were monitored. It was demonstrated that the accordance rate of group 3 and group 2 with group 1 was 56.25% and 43.75%, respectively. The maternal-fetal transmission rate in the group 1, 2 and 3 was 19.00%, 40.58% and 46.15%, respectively, with a significant difference found between group 2, 3 and group 1 (P < 0.01). Incidence of spontaneous abortion, fetal death, fetal abnormality and neonatal death in group 1, 2 and 3 was 10.00%, 15.94% and 30.77%, respectively, and that of group 3, 2 was 4 and 2 times as much as that of group 1, respectively (OR = 4.00, P < 0.001; OR = 2.343, P < 005, respectively). It was concluded that HCMV-IgM(+) can only be considered as an screening indicator for pregnant women with HCMV infection, while IgM(+) combined with serous DNA(+) or mRNA(+) indicates active infection and has a high incidence of maternal-fetal transmission and abnormal pregnancy outcome.
Adult ; Antibodies, Viral ; blood ; Cytomegalovirus ; immunology ; isolation & purification ; Cytomegalovirus Infections ; diagnosis ; transmission ; virology ; DNA, Viral ; blood ; Enzyme-Linked Immunosorbent Assay ; Evaluation Studies as Topic ; Female ; Humans ; Immunoglobulin M ; blood ; Infectious Disease Transmission, Vertical ; Polymerase Chain Reaction ; methods ; Pregnancy ; Pregnancy Complications, Infectious ; virology ; Pregnancy Outcome ; Prenatal Diagnosis ; Reverse Transcriptase Polymerase Chain Reaction

2

Cite

Share

Anginogenesis of eutopic and ectopic endometria in endometriosis.

Yi LIU ; Liqun LÜ ; Guijin ZHU

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):190-191.

In order to study the angiogenesis in endometriosis, the samples of eutopic and ectopic endometria from patients with endometriosis were quantitatively analyzed by color morphometric image system (CMIS) for vascular surface area, and by examining endometrial blood vessel for microvessel density (MVD). The results showed that within each menstrual phase the vascular surface area and MVD were significantly higher in ectopic endometria with endometriosis than those in eutopic endometria with endometriosis or normal endometrium (P < 0.05). It is concluded that angiogenesis might be involved in the development of endometriosis.
Adult ; Endometriosis ; etiology ; pathology ; Endometrium ; blood supply ; Female ; Humans ; Menstrual Cycle ; Neovascularization, Pathologic ; Pelvis

3

Cite

Share

Detection of embryo sex chromosome by dual color fluorescent in-situ hybridization.

Qun LIU ; Guijin ZHU

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):187-189.

In order to evaluate the effects of sex chromosomal mosaicism on the accuracy of single-cell gender diagnosis, sex chromosomes of 21 normal fertilized embryos were detected by dual color fluorescent in-situ hybridization (FISH). The results showed that 4 embryos had sex chromosomal mosaicism (19%) and the remaining 17 showed uniformly XX or XY signals in all blastomeres. In conclusion, identification of sex by dual color FISH analysis of a single cell was accurate and efficient, and sex chromosomal mosaicism would not affect preimplantation gender diagnosis.
Blastocyst ; Chromosomes, Human, X ; genetics ; Chromosomes, Human, Y ; genetics ; Embryonic Development ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Mosaicism ; genetics ; Pregnancy ; Preimplantation Diagnosis ; methods ; Sex Chromosome Aberrations ; embryology ; Sex Chromosome Disorders ; diagnosis ; Sex Determination Processes

4

Cite

Share

The expression of PLF in placenta in pregnancy-induced hypertension and the relationship between the PLF and serum VCAM-1.

Xiaoyan XU ; Wenpei XIANG ; Hanping CHEN

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):184-186.

To study the expression of placental isoferritin (PLF) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of PLF and the amount of vascular cell adhesion molecule-1 (VCAM-1) in serum, immunohistochemical technique was used to detect the expression of PLF in placenta tissue in 45 PIH patients (PIH group) and 15 normal pregnant women (normal group). High resolution pathological image analysis system (HPIAS-100) was employed to determine the quantity of PLF. The VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay (ELISA). The results showed that the levels of PLF expressions in moderate and severe PIH patients were significantly lower than that of normal group (P < 0.01). The serum VCAM-1 was significantly decreased in PIH group (1310 +/- 177 rho/ng/ml) than that of normal group (609 +/- 72 rho/ng/ml, P < 0.01). The significant negative correlation existed between the expression of PLF in placental tissue and the serum VACM-1 (r = -0.58, P < 0.01). It was concluded that the level of PLF expression in PIH decreases and is negatively correlated with the amount of serum VCAM-1, indicating that these may be involved in the pathogenesis of PIH.
Adult ; Enzyme-Linked Immunosorbent Assay ; Female ; Ferritins ; metabolism ; Humans ; Immunohistochemistry ; Placenta ; metabolism ; Pre-Eclampsia ; etiology ; metabolism ; Pregnancy ; Vascular Cell Adhesion Molecule-1 ; blood

5

Cite

Share

Comparative study of 3D-SPGR vs 2D-SE T1WI after enhancement in the brain.

Wenzhen ZHU ; Jianpin QI ; Chengyuan WANG

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):180-183.

The utility of three-dimensional spoiled gradient recalled acquisition in steady state (3D-SPGR) imaging in the cerebral diseases was evaluated and 3D-SPGR after enhancement in depicting contrast enhancement of all lesions and 2D-SE T1WI comparatively analyzed. 117 patients were subjected to MRI by a GE 1.5T MR system. After performance of axial T1WI and T2WI in all patients, MRA (3D-MOTSA) images were acquired in 6 cases (8 lesions) of aneurysms. After enhancement, 3D-SPGR images were obtained in all the remaining patients. Quality parameters (SNR, C and CNR) were calculated on enhanced 2D-SE T1WI and 3D-SPGR images. And a four-point scale was used to measure the signal intensity of the main lesions on both sequences, then statistical analysis of the average score was performed with "t" test. Except for aneurysms, 2D-SE T1WI detected 134 lesions and 3D-SPGR disclosed 147 lesions. It was found that there was no statistically significant difference between the two average scores as determined by the "t" test (t = 1,894, P > 0.05). The enhancement degree of the main lesion was equivalent on 3D-SPGR and 2D-SE T1WI. Quality parameters (SNR, C and CNR) on 2D-SE T1WI were much larger than that of 3D-SPGR, increasing by an average of 57%, 20% and 97% respectively. 3D-SPGR imaging with MPR could clearly depict vascularity related to neoplasms in 20 cases and demonstrate shifted, deformed and blocked vessels involved by tumors. Six cases of large aneurysms (8 lesions) were visualized more clearly on 3D-SPGR than MRA (3D-MOTSA): 3D-SPGR could display aneurysm necks and differentiate thrombosed portion from the patent lumen, and disclose relationship of aneurysm to surrounding structures. It was concluded that enhanced 3D-SPGR played an important role in the depiction of the cerebral lesions and was superior to 2D-SE T1WI in many aspects.
Adolescent ; Adult ; Aged ; Angiography, Digital Subtraction ; methods ; Brain ; pathology ; Brain Neoplasms ; diagnosis ; secondary ; Child ; Child, Preschool ; Echo-Planar Imaging ; Female ; Humans ; Image Enhancement ; methods ; Imaging, Three-Dimensional ; methods ; Intracranial Aneurysm ; diagnosis ; Magnetic Resonance Angiography ; Magnetic Resonance Imaging ; methods ; Male

6

Cite

Share

Effects of ropivacaine and bupivacaine on rabbit myocardial energetic metabolism and mitochondria oxidation.

Shihai ZHANG ; Shanglong YAO ; Qing LI

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):178-183.

To compare the cardiotoxicity induced by ropivacaine and bupivacaine and to investigate the mechanism of cardiotoxicity, 24 mature New Zealand rabbits were divided randomly into control group (group C), ropivacaine group (group R) and bupivacaine group (group B). Hearts were drawn out rapidly from the anesthetized animals and cardiac perfusion was performed immediately. Ropivacaine 500 ng/ml (group R) or bupivacaine 500 ng/ml (group B) was added to the perfusion solution. Ventricular myocardial ATP, ADP and AMP were measured with high performance liquid chromatogram. The ability of myocardial mitochondria oxidation to pyruvate or palmitoylcarnitine was detected with Clark electrode. Our results showed that myocardial ATP and ADP decreased significantly (P < 0.05) in group R and most significantly (P < 0.01) in group B as compared with group C. Myocardial ATP and ADP decreased most significantly (P < 0.01) in group B as compared with group R. The changes of myocardial AMP revealed significant difference among three groups. The changes of pyruvate oxidation exhibited no significant difference among the three groups. Palmitoylcarnitine oxidation decreased markedly (P < 0.05) in group R and most significantly (P < 0.01) in group B as compared with group C. The present study indicated that the inhibition of lipid substrate oxidation may be responsible for the cardiotoxicity induced by bupivacaine and ropivacaine. The cardiotoxicity induced by ropivacaine is far more less than bupivacaine.
Adenosine Diphosphate ; metabolism ; Adenosine Triphosphate ; metabolism ; Amides ; toxicity ; Anesthetics, Local ; toxicity ; Animals ; Bupivacaine ; toxicity ; Energy Metabolism ; In Vitro Techniques ; Mitochondria, Heart ; metabolism ; Myocardium ; metabolism ; Oxidation-Reduction ; Rabbits ; Random Allocation

7

Cite

Share

Prevention and treatment of postoperative complications of the penile elongation.

Muosheng YU ; Shengguo SHAN ; Yueqiang ZHAO ; Xiaowei WU ; Lichun ZHOU ; Daochou LONG

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):176-177.

To explore the causes of the postoperative complications of the penile elongation and the measures to prevent them in order to raise the success rate of the penile elongation. 1,000 patients who had received the penile elongation were reviewed and analyzed for the causes of postoperative complications, and the measures of prevention and treatment were discussed. Our results showed that, of the 1,000 cases, 64 had the postoperative complications, including 20 cases of edema of prepuce, 15 cases of flap necrosis, 12 hematoma, 9 infections, and 8 cases of fat and clumsy penis. It is concluded that correct operative manipulation, strict aseptic measures and necessary postoperative care and management could avoid or reduce the postoperative complications. When complications happened, a satisfactory result can be achieved with timely and correct treatment in the majority of the patients.
Adolescent ; Adult ; Aged ; Edema ; etiology ; prevention & control ; Hematoma ; prevention & control ; Humans ; Male ; Middle Aged ; Penis ; abnormalities ; injuries ; surgery ; Postoperative Complications ; prevention & control ; therapy ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps ; Surgical Wound Infection ; etiology ; prevention & control ; Urologic Surgical Procedures, Male ; methods

8

Cite

Share

Expression of telomerase gene mTR in the testis of SD rats and its significance.

Min FAN ; Xiaochun CHEN ; Zhewei YE ; Yali XIONG ; Fuqing ZENG ; Gongcheng LU

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):173-175.

To study the expression of mTR gene in the testis of SD rats with varied ages and its significance, in situ hybridization (ISH) techniques were applied to detect the expression of telomerase gene mTR mRNA in the testis of SD rats. The expression of mTR was found in testes of different-age male SD rats. There was a positive correlation between the expression of mTR and the location of germ cells (spermatogonia, spermatocyte, spermatid). In Setoli cells, leydig cell and spermatozoa, no telomerase mTR was detectable. Type A spermatogonia expressed the highest level of telomerase mTR mRNA. It was suggested that the expression of mTR gene in the testis of SD rats is of lifetime and coincides with the telomerase activity.
Animals ; Cell Differentiation ; Gene Expression ; In Situ Hybridization ; Male ; RNA ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Rats ; Rats, Sprague-Dawley ; Spermatocytes ; metabolism ; Spermatogonia ; metabolism ; Telomerase ; biosynthesis ; genetics ; metabolism ; Testis ; metabolism

9

Cite

Share

Effect of Ad-p16 combined with CDDP or As2O3 on human bladder cancer cells.

Zhaohui ZHU ; Shi'an XING ; Chen LIN

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):169-172.

To evaluate the therapeutic efficiency of combined use of p16-expressing adenovirus and chemotherapeutic agents CDDP or As2O3 on human bladder cancer cell line EJ, the human bladder cancer cell line EJ were transfected with adenovirus-mediated p16 gene (Ad-p16), with administration of cisplatin (CDDP) or arsenic trioxide (As2O3). The cell growth, morphological changes, cell cycle, apoptosis and molecular changes were measured using cell counting, reverse microscopy, flow cytometry, cloning formation, immunocytochemical assays and in vivo therapy experiments to evaluate the therapeutic efficacy of such combined regimen. Ad-p16 transfer and CDDP or As2O3 administration to EJ cells could exert substantially stronger therapeutic effects than the single agent treatment. Especially in in vivo experiments, combined administration of p16 and CDDP or As2O3 induced almost tumor diminish compared to the partial tumor diminish induced by single agent. Moreover, delivery of Ad-p16, or administration of minimal-dose CDDP or As2O3 or combined regimen could induce massive apoptosis of EJ cell. Cell cycle analysis demonstrated that administration of CDDP or As2O3 remarkably arrested EJ cell in G1 prior to apoptotic cell death. When treated with combined regimen, cells were arrested in G1 to a greater extent prior to apoptotic cell death. It is concluded that after introduction into EJ cell, Ad-p16 shows enhanced therapeutic efficacy for EJ cell when used in combination with CDDP or As2O3.
Adenoviridae ; genetics ; Apoptosis ; drug effects ; Arsenicals ; pharmacology ; Cell Cycle ; drug effects ; Cisplatin ; pharmacology ; Cyclin-Dependent Kinase Inhibitor p16 ; biosynthesis ; genetics ; Genetic Vectors ; Humans ; Oxides ; pharmacology ; Transfection ; methods ; Tumor Cells, Cultured ; Urinary Bladder Neoplasms ; metabolism ; pathology

10

Cite

Share

Growth inhibition and apoptosis induction in human hepatoma cells by tanshinone II A.

Zhongzhi TANG ; Ying TANG ; Libo FU

Journal of Huazhong University of Science and Technology (Medical Sciences).2003;23(2):166-172.

In order to study the effect of tanshinone II A on growth and apoptosis in human hepatoma cell line BEL-7402 in vitro, the human hepatoma cell line BEL-7402 was treated with tanshinone II A at various concentrations for 72 h. Growth suppression was evaluated by MTT assay; apoptosis-related alterations in morphology and biochemistry were ascertained under cytochemical staining (Hoechst 33258), transmission electron microscopy (TEM), and DNA agarose gel electrophoresis. Apoptotic rate was quantified by flow cytometry (FCM). The results showed that Tanshinone II A could inhibit the growth of hepatoma cells in a dose-dependent manner, with IC50 value being 6.28 micrograms/ml. After treatment with 1-10 micrograms/ml tanshinone II A for 72 h, BEL-7402 cells apoptosis with nuclear chromatin condensation and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed. DNA ladder could be demonstrated on DNA electrophoresis. FCM analysis showed hypodiploid peaks on histogram, and the apoptotic rates at 5 micrograms/ml concentration for 12 h, 24 h, 36 h, 48 h and 72 h were (2.32 +/- 0.16)%, (3.01 +/- 0.35)%, (3.87 +/- 0.43)%, (6.73 +/- 0.58)% and (20.85 +/- 1.74)% respectively, which were all significantly higher than those in the control group (1.07 +/- 0.13)%. It is concluded that Tanshinone II A could induce human hepatoma cell line BEL-7402 apoptosis, which may be related to the mechanism of growth inhibition.
Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Carcinoma, Hepatocellular ; pathology ; Cell Division ; drug effects ; Cell Line, Tumor ; Diterpenes, Abietane ; Drugs, Chinese Herbal ; pharmacology ; Flow Cytometry ; Humans ; Liver Neoplasms ; pathology ; Phenanthrenes ; pharmacology

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share