Journal Detail Information

1

Cite

Share

Treatment of burn cicatricial foot drop with Ilizarov fixator.

Yu-ming SHEN ; Lei HUANG ; Xiao-hua HU ; Ming LI ; Guo-an ZHANG

Chinese Journal of Burns.2008;24(4):287-289.

OBJECTIVETo investigate the efficacy of Ilizarov fixator on cicatricial foot drop after burn.

METHODSSix patients with cicatricial foot drop after burn were treated with Ilizarov fixator during June 2004 approximately October 2007, the fixator was set on the leg and foot by fixed bone needles. Nuts on the threaded rod were turned from 3 post operation day, 2 approximately 4 rounds per time and 4 times per day in the first week, then 1 approximately 2 rounds per time and 4 times a day, which corrected the deformity of talipes equinus by shortening or lengthening the thread rod in the front and at the back. Ankle joint was maintained in neutral position for 2 approximately 3 months after effective correction. Weight carrying for patients was increased gradually after removal of fixator. Ankle joint was maintained in neutral position with fixator at least three months. Patients were followed up 5 approximately 10 months.

RESULTSAnkle joints from all patients were restored to neutral position after application with fixator for 4 approximately 6 weeks. All patients achieved 0 degrees dorsiflexion in weight carrying for whole planta pedis after use of fixator for 12 approximately 15 weeks with good locomotion function.

CONCLUSIONIlizarov fixator is safe and mini-injury, which is an effective method for treatment of cicatricial foot drop.

Adolescent ; Adult ; Burns ; complications ; surgery ; Child ; Cicatrix ; etiology ; surgery ; Clubfoot ; etiology ; surgery ; Contracture ; etiology ; surgery ; External Fixators ; Female ; Foot Deformities, Acquired ; etiology ; surgery ; Humans ; Male ; Middle Aged ; Young Adult

2

Cite

Share

The protection of heme oxygenase-1 from acute cardiocyte injury in rats.

Ren-qiang YANG ; Xiao-shu CHENG ; Chen LIU ; Ling WANG ; Ping LI ; Yan-qing WU ; Qing-hua WU ; Hai SU ; Yu-cheng DAI

Chinese Journal of Burns.2008;24(4):283-286.

OBJECTIVETo observe the protection of Heme oxygenase-1 (HO-1) from lipopolysaccharide (LPS)-induced cardiocyte injury and its mechanism.

METHODSCardiocyte was isolated from SD neonate rat and cultured in vitro, and was divided into control group (normal culture), LPS group (with stimulation of 30 micromoL/L LPS for 1 hour), LPS + Hemin group (with same treatment to LPS group after stimulation of 5 micromoL/L Hemin for 1 hour), and LPS + ZnPP group (with same treatment to LPS group after stimulation of 3 micromoL/L ZnPP for 1 hour). The level of lactic-dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) were measured by thio-barbituric acid and xanthine oxidase techniques. The cell heart rhythm, survival rate and apoptosis rate were examined. The expressions of nuclear factor kappaB (NF-kappaB), HO-1 and tumor necrosis factor-alpha (TNF-alpha) were measured with Western blotting. The HO-1 mRNA was examined by RT-PCR.

RESULTSThe level of LDH and MDA in LPS, LPS + Hemin, and LPS + ZnPP groups were (113 +/- 15), (79 +/- 13), (154 +/- 22) U/L, and (1.88 +/- 0.36), (1.16 +/- 0.32), (2.84 +/- 0.44) mmoL/L respectively, which were all obviously higher than those in control group [(69 +/- 10) U/L, (0.87 +/- 0.25) mmol/L, P < 0.05]. The level of SOD in LPS, PS + Hemin, and LPS + ZnPP groups (17.8 +/- 1.8, 22.5 +/- 2.4, 13.4 +/- 1.5 U/mL, respectively) was all obviously lower than that in control group (24.3 +/- 3.6 U/mL, P < 0.05). The apoptosis rate and heart rhythm were obviously higher and survival rate significantly lower in LPS, LPS + Hemin, and LPS + ZnPP groups than those in control group (P < 0.05). The level of HO-1mRNA in LPS, LPS + Hemin, and LPS + ZnPP groups was higher than that in control group (P < 0.01), among which LPS + Hemin group was the highest. The level of HO-1, TNF-alpha and NF-kappaB in LPS, LPS + Hemin, and LPS + ZnPP groups was higher than those in control group (P < 0.05), among which the level of HO-1 protein in LPS + Hemin group was the highest, the level of TNF-alpha and NF-kappaB in LPS + ZnPP group was highest.

CONCLUSIONLPS can induce cardiocyte injury, which can be inhibited through the anti-inflammatory, anti-oxidant, and anti-apoptosis functions by HO-1.

Animals ; Caspase 3 ; metabolism ; Cells, Cultured ; Heme Oxygenase (Decyclizing) ; metabolism ; Hemin ; pharmacology ; L-Lactate Dehydrogenase ; metabolism ; Lipopolysaccharides ; Malondialdehyde ; metabolism ; Myocytes, Cardiac ; metabolism ; NF-kappa B ; metabolism ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

3

Cite

Share

Study on activation of mitogen-activated protein kinase pathway in lung tissue of rats with smoke inhalation injury.

Hui AN ; Lei SUN ; Yan-hong ZHOU ; Jia CAO

Chinese Journal of Burns.2008;24(4):278-282.

OBJECTIVETo investigate the activation of mitogen-activated protein kinase (MAPK) pathway and the expression of inflammatory cytokine in lung tissue of rats with smoke inhalation injury, and to explore the injury mechanism.

METHODSThe model of smoke inhalation injury in airtight cabin was established. Thirty-six SD rats were randomly divided into normal control group (n = 6, NC group) and inhalation injury group (n = 30, II group). The rats in II group were observed at 1, 6, 24, 72 post injury hour (PIH) and 7 post injury day (PID). The pathological changes in lung tissue were observed by optical microscope. The contents of TNF-alpha, MIP-2, IL-1beta in lung tissue homogenate were examined. The level of p38MAPK, JNK, ERK1/2 and their phosphorylation in lung tissue were measured by Western blotting. The contents of TNF-alpha, MIP-2, and IL-1beta and granulocytes counts in BALF (bronchoalveolar lavage fluid) were detected by ELISA.

RESULTSPathological change of smoke inhalation was similar to acute lung injury. The contents of TNF-alpha and IL-1beta of lung tissue and BALF in II group at 1 PIH were higher than those in NC group (P < 0.01). The levels of MIP-2 of lung tissue in II group at each time point were similar to that in NC group (P > 0.05), while that of BALF in II group at 1 PIH were obviously higher than that in NC group (P < 0.01). Compared with those in NC group, the levels of p38MAPK, JNK, ERK1/2 in II group at each time point were similar, and their phosphorylation levels increased with different degree. Compared with that in NC group [(0.61 +/- 0.09) x 10(6) cells/L], the total number of granulocytes in II group was significantly decreased at 1PIH [(0.36 +/- 0.08) x 10(6) cells/L], increased on 7PID [(1.71 +/- 0.67) x 10(6) cells/L, P < 0.05]. The number of neutrophils in II group during 6PIH approximately 7 PID were more than that in NC group. The number of lymphocyte in II group at 1PIH less than that in NC group (P < 0.05), which increased gradually during 6 PIH approximately 7PID. There were no obvious difference in number of lymphocyte between two groups (P > 0.05).

CONCLUSIONThe obvious inflammatory response in lung tissue can be induced by the poisonous gas released from the combustion of nonmetal materials in airtight cabin, and activate the expression of kinases in MAPK pathway, which may be one of mechanisms for lung injury induced by poisonous gas.

Animals ; Disease Models, Animal ; Interleukin-1 ; metabolism ; Lung ; metabolism ; pathology ; Lung Injury ; metabolism ; pathology ; Male ; Mitogen-Activated Protein Kinases ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Smoke Inhalation Injury ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

4

Cite

Share

Effect of transfection of recombinant human endothelial nitric oxide synthase gene on hypertrophic scar fibroblasts in vitro.

Ping YANG ; Ai-li WANG ; De-wu LIU ; Shun XU ; Yao-hui GU ; Jing HUANG ; Bo CHEN ; Qian-cheng LUO ; Qing JIA ; Zhi-hong WU

Chinese Journal of Burns.2008;24(4):275-277.

OBJECTIVETo investigate the feasibility of transfection of recombinant human endothelial nitric oxide synthase (eNOS) into human hypertrophic scar fibroblasts (HSFbs), and to observe NO secretion and the synthesis of collagen I and III.

METHODSRecombinant human eNOS with karyocyte expressive vector was constructed in vitro, then was transfected into HSFbs which was isolated from hypertrophic scar tissues and cultured in vitro (T group). The HSFbs untransfected (normal culture) or transfected with empty-vector was used as control group and empty-vector group respectively. The mRNA expression of eNOS, collagen I and III was determined by Realtime PCR. The content of NO was determined by NO assay kit.

RESULTSThe expression of eNOS mRNA in T group was 5.92 +/- 0.21, which was obviously higher than that in empty-vector group (0.98 +/- 0.13, P < 0.05). The expression of collagen I mRNA (0.76 +/- 0.15), and collagen III (0.79 +/- 0.08) in T group was significantly lower than those in empty-vector group (0.98 +/- 0.15, 1.02 +/- 0.12, P < 0.05, respectively). The content of NO in T group (36.1 +/- 0.8 micromol/L) was obviously higher than that in empty-vector group (28.4 +/- 1.0 micromol/L, P < 0.01) and control group (27.7 +/- 1.3 micromol/L, P < 0.01).

CONCLUSIONHSFbs can be the target cells for eNOS gene transfection. The transfected cells can express eNOS and produce NO, which inhibit the synthesis of collagen.

Cicatrix, Hypertrophic ; metabolism ; Collagen Type I ; genetics ; metabolism ; Collagen Type III ; metabolism ; Fibroblasts ; metabolism ; Humans ; In Vitro Techniques ; Nitric Oxide ; metabolism ; Nitric Oxide Synthase Type III ; genetics ; RNA, Messenger ; metabolism ; Transfection

5

Cite

Share

Construction of tissue-engineering skin with carrier of active composite dermal matrix.

Zhong-feng MA ; Jia-ke CHAI ; Hong-ming YANG ; Li-ming LIANG ; Ming-huo XU

Chinese Journal of Burns.2008;24(4):272-274.

OBJECTIVETo construct of tissue engineering skin including active composite dermal matrix.

METHODSThe human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.

RESULTSThe structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.

CONCLUSIONFibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.

Animals ; Cattle ; Cell Culture Techniques ; Collagen Type I ; Dermis ; transplantation ; Epidermis ; cytology ; Extracellular Matrix ; transplantation ; Fibroblasts ; cytology ; Humans ; Skin ; cytology ; Skin, Artificial ; Swine ; Tissue Engineering

6

Cite

Share

Repair of claw hand deformity after burn.

Pi-hong ZHANG ; Xiao-yuan HUANG ; Peng-ju FAN ; Li-cheng REN ; Jian-hong LONG

Chinese Journal of Burns.2008;24(4):268-271.

OBJECTIVETo summarize methods for repair of claw hand deformity after burn.

METHODSNinety-seven patients with 136 claw hands after burn hospitalized from May 1992 to May 2007 were repaired with skin grafting (104 hands) and transposition of skin flap (32 hands), among which 21 hands were minor-grade, 92 hands moderate, 23 hands severe. The metacarpophalangeal joint was repaired after scar release in dorsum of hand with manual extraction reduction, release of collateral ligament and joint capsula, separation of adhesion in joint, tendon lengthening for obvious contracture. Restitution of finger flexion deformity, lysis of adhesion and grafting among first web and finger webs, repair of central slip extensor tendon or phalangeal arthrodesis were performed according to the abnormal condition after lysis of dorsal scar of hand. The metacarpophalangeal joint from 31 patients were not repaired with above methods for severe finger flexion deformity, their palmar scar were loosened and transplanted firstly, then scar in dorsum of hand were loosened, metacarpophalangeal joint were repaired, flap or skin were transferred or transplanted. General rehabilitation were performed routinely after operation.

RESULTSThe ending of flaps (4 hands) due to the scar were necrosis after transposition and healed through dressing change, other skins or flaps all survived. Most articular deformities were corrected completely or basically. Functions including palmar opposition, grasp were also recovered with satisfactory results.

CONCLUSIONSkin transplantation and transferring of skin flap with overall planning and individual isatin are the key points for repair of claw hand after burn.

Adolescent ; Adult ; Burns ; complications ; Child ; Child, Preschool ; Cicatrix ; etiology ; surgery ; Female ; Hand Deformities, Acquired ; surgery ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; Surgical Flaps ; Young Adult

7

Cite

Share

Study on crosstalk between phosphatidylinositol 3 -kinase/Akt pathway and p38 mitogen-activated protein kinase pathway in cardiomyocyte with challenge of burn serum.

Gen-fa LV ; Bi CHEN ; Wan-fu ZHANG ; Yun-chuan WANG ; Xiong-xiang ZHU ; Da-hai HU

Chinese Journal of Burns.2008;24(4):263-267.

OBJECTIVETo investigate the possibility of crosstalk between phosphatidylinositol 3-kinase (PI3-K)/Akt pathway and p38 mitogen-activated protein kinase (p38MAPK) pathway in cardiomyocyte with challenge of burn serum, and to explore their influence on cardiomyocyte injury after burn.

METHODSThe model of murine cardiomyocyte with stimulation of burn serum was established. (1) The level of Akt and p38 phosphorylation in cardiomyocyte were examined with stimulation of 10% burn serum before stimulation and 1, 3, 6, 12, 24 hour after stimulation. (2) The levels of Akt and p38 phosphorylation in cardiomyocyte were determined with stimulation of burn serum (at concentration of 5%, 10%, 20%) or 10% burn serum plus insulin (at concentration of 1 x 10(-8), 1 x 10(-7), 1 x 10(-6)mol/L). The content of creatine kinase (CK) in supernate was also detected. (3) Addition to the inhibitor of p38 MAPK pathway (SB203580) and PI3K/Akt pathway (LY294002), the level of p38MAPK, PI3K/Akt and the content of CK in supernate were determined.

RESULTS(1) The level of p-p38 in cardiomyocyte was 4.0 +/- 0.8, 3.6 +/- 0.8, 5.1 +/- 1.6, 2.4 +/- 0.5, 3.0 +/- 0.6 at 1, 3, 6, 12, 24 hour (s) after stimulation of burn serum, which was obviously higher than that immediate after stimulation (1.0, P < 0.01). The level of p-Akt was 0.15 +/- 0.07, 0.64 +/- 0.10, 0.26 +/- 0.08, 0.38 +/- 0.11, 0.59 +/- 0.13, which was obviously lower than that before stimulation (1.00, P < 0.01). (2) With stimulation of different concentration of burn serum or burn serum plus insulin, the level of p-Akt and p-p38 changed in the opposite directions comparatively. The content of CK increased along with increase of burn serum concentration, but decreased obviously with treatment of insulin (P < 0.05 or 0.01). (3) Low level of p38 induced by burn serum was increased after treatment of LY294002, which neutralized the protection of insulin (P < 0.01). Low level of p-Akt induced by burn serum increased after treatment of SB203580 (P < 0.01), which inhibited the release of CK induced by burn serum.

CONCLUSIONThere is being crosstalk between PI3K/Akt pathway and p38 MAPK pathway in cardiomyocytes with challenge of burn serum, which may regulate cardiomyocytes.

Animals ; Burns ; blood ; Male ; Myocytes, Cardiac ; metabolism ; Phosphatidylinositol 3-Kinases ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Rats ; Rats, Sprague-Dawley ; Serum ; Signal Transduction ; p38 Mitogen-Activated Protein Kinases ; metabolism

8

Cite

Share

The effect of stimulation of toss simulated at sea on shock in severe burn rabbits.

Guang WANG ; Bing-qian ZHANG ; Jing RUAN ; Zhong-hua LUO ; Yue-sheng HUANG

Chinese Journal of Burns.2008;24(4):258-262.

OBJECTIVETo investigate the effect of stimulation of toss simulated at sea on shock in severe burn rabbits.

METHODSOne hundred and thirty-two rabbits were randomly divided into normal control group (NC group, n = 6), toss group (T group, with treatment of continuous toss, n = 42), burn group (B group, with treatment of burn, n = 42), burn and toss (BT group, with treatment of continuous toss after burn, n = 42). The level of Cr, BUN, HCT and LA from blood samples in T, BT, B groups were observed at 2, 6, 8, 12, 24, 36, 48 post treatment hour (PTH). The changes in urinary volume was measured during 48 PTH. The histopathologic changes in kidney were observed at above-mentioned time points. The above indices in NC group were also observed.

RESULTSThe mean urinary volume in B group during the first and second 24 PTH was (2.59 +/- 0.23) and (2.86 +/- 0.29) mL/h, while that in BT group was (1.61 +/- 0.13) and (1.66 +/- 0.16) mL/h respectively, which were all lower than those in NC group (6.06 +/- 0.18 mL/h, P < 0.01). The levels of HCT and LA in BT group were obviously higher than those in B group at each time point. The levels of Cr and BUN in BT group at 24, 36, 48 PTH were significantly higher than those in B group. The histopathological observation showed the capillary vessels and mesenchymal cells of kidney glomerulus were congestive, epithelial cells in kidney tubules were swollen. The infiltration degree of inflammatory cells in kidney tubule, and the pathological changes of erythrocyte cast in BT group were more serious than those in B group.

CONCLUSIONThe toss simulated at sea can significantly aggravate shock and the renal damages in severe burn rabbits.

Animals ; Burns ; complications ; Creatinine ; blood ; Erythrocyte Volume ; Kidney ; pathology ; Male ; Motion ; Physical Stimulation ; Rabbits ; Random Allocation ; Shock ; etiology ; pathology

9

Cite

Share

Improving myocardial mechanics parameters of severe burn rabbits with oral fluid resuscitation.

Jing RUAN ; Bing-qian ZHANG ; Guang WANG ; Zhong-hua LUO ; Qing-yi ZHENG ; Jian-sheng ZHENG ; Yue-sheng HUANG ; Rong XIAO

Chinese Journal of Burns.2008;24(4):254-257.

OBJECTIVETo investigate the protective effect of oral fluid resuscitation on cardiac function in severe burn rabbits.

METHODSOne hundred and fifty rabbits were randomly divided into normal control group (NC group, n = 6, without treatment), burn group (B group, n = 42, without fluid therapy), immediate oral fluid resuscitation group (C group, n = 42), delayed oral fluid resuscitation group (D group, n = 30) and delayed and rapid oral fluid resuscitation group (E group, n = 30). The rabbits in B, C, D, E groups were subjected to 40% TBSA full-thickness burn, then were treated with fluid therapy immediately after burn (C group), at 6 hour after burn (D, E groups). The myocardial mechanics parameters including mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), LV +/- dp/dt max were observed at 2, 6, 8, 12, 24, 36 and 48 post burn hour (PBH). Urine output was also examined.

RESULTSThe level of LVSP, LV +/- dp/dt max in B roup were significantly lower than those in NC group. The level of LVSP, LV +/- dp/dt max in the C and E group were singnificantly increased during 24 hour after burn. The level of LV + dp/dt max and LV-dp/dt max in C group peaked at 8 PBH (892 +/- 116 kPa/s) and at 6PBH (724 +/- 149 kPa/s) respectively. The levels of LV +/- dp/dt max, LVSP in D group at each time point were similar to B group (P > 0.05). Both the levels of LV +/- dp/dt max in E group peaked at 8 PBH. The level of LVEDP was no obvious difference between B and other groups at each time point (P > 0.05). The changes of MAP and urine output on 24 PBH in each group were similar to above indices.

CONCLUSIONEffective oral fluid therapy in severe burn rabbits during 24 hours after burn can ameliorate myocardial mechanics parameters. The amount of fluid resuscitation can be estimated according to relevant formula for delayed fluid resuscitation in burn rabbits.

Animals ; Burns ; physiopathology ; therapy ; Cardiac Output ; Fluid Therapy ; Heart Rate ; Male ; Rabbits ; Random Allocation ; Ventricular Function

10

Cite

Share

Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe burn rat.

Liang QIAO ; Ke-jian YUAN ; Hui-zhong YANG ; He-liang DONG ; Wen-kui WANG ; Wei-shi XU

Chinese Journal of Burns.2008;24(4):251-253.

OBJECTIVETo investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe burn rats at early stage.

METHODSSprague-Dawley rats inflicted with 30% TBSA full-thickness burn were randomly divided into A group (n = 36, fluid resuscitation with administration of lytic cocktail), B group (n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of oxygen (PaO2) in arterial blood and histopathologic changes on 3, 5, 7, 10 post burn day (PBD). The levels of malonyldialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-alpha(TNF-alpha) and interferon-gamma (IFN-gamma) in lung tissue were measured at the same time points.

RESULTSThe PaO2 level in A group on 3 PBD (12.58 +/- 0.41 kPa) was significantly higher than that in B group (8.86 +/- 0.23 kPa, P < 0.01). Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point (P < 0.05 or 0.01), the levels of TNF-alpha on 3, 5, 7 PBD (P < 0.05 or 0.01) and IFN-gamma on 5, 7, 14 PBD (P < 0.01) were also decreased in A group. Swollen lung mesenchyme was alleviated, infiltration of inflammatory cell was lessened in A group.

CONCLUSIONLytic cocktail combined with immediate fluid resuscitation can inhibit stess response, downregulate the expression of inflammatory factor, ameliorate lung function in severe burn rat at early stage.

Animals ; Burns ; complications ; therapy ; Fluid Therapy ; Interferon-gamma ; metabolism ; Lung Injury ; etiology ; metabolism ; therapy ; Malondialdehyde ; metabolism ; Meperidine ; therapeutic use ; Peroxidase ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; metabolism

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share