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IL-33 and sST2 levels in Serum of Children with Henoch-Schnlein Purpura and Their Clinical Significance.

Journal of Experimental Hematology.2017;25(2):517-521. doi:10.7534/j.issn.1009-2137.2017.02.038

OBJECTIVETo investigate the serum IL-33 and sST2 levels in children with Henoch-Schonlein purpura (HSP), and explore their clinical significance.

METHODSTotal 27 HSP patients and 22 healthy controls were enrolled in present study. The expressions of IL-33 and sST2 were measured by enzyme linked immunosorbent assay (ELISA). Using real-time quantitative polymerase chain reaction (RT-PCR), the mRNA expression of IL-33 and sST2 were detected in all subjects.

RESULTSThe level of the IL-33 in the serum of HSP group and control group was 365.5±160.6 pg/ml and 175.9±92.8 pg/ml(P< 0.05). The level of the sST2 was increased in the serum of HSP group (1788.6±523.8 pg/ml) as compared with that in control group (1083.6±489.6 pg/ml)(P>0.05), but the ratio of sST2/IL-33 in HSP patients was much lower than that in the controls(P<0.05), IL-33 and sST2 mRNA levels were up-regulated in HSP patients by 5.47±1.97-fold(P<0.05) and 3.13±2.01-fold(P<0.05) compared with controls, but sST2/IL-33 significantly decreased in HSP patients(P<0.05).

CONCLUSIONThe levels of IL-33 and sST2 increase in the serum of HSP patients, but the ratio of sST2/IL-33 is much lower than that in control.

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Prognosis and Survival Analysis of Patients with Essential Thrombocythemia.

Mo CHEN ; Li-Jun HOU ; Zeng-Xiang LIU ; Hong-Bing LIU ; Qiao-Dan LIU

Journal of Experimental Hematology.2017;25(2):510-516. doi:10.7534/j.issn.1009-2137.2017.02.037

OBJECTIVETo investigate the survival status and prognosis of patients with essential thrombocythemia(ET) and analyze the prognostic factors for the patients' survival, so as to provide a evidence for clinical treatment and prognosis evaluation.

METHODSA retrospective analysis of 118 patients with ET was conducted in the Fifth Affiliated Hospital of Sun Yat-Sen University and Zhongshan Municipale People's Hospital from December 2002 to December 2013. The clinical characteristics were summarized, such as the survival curve and multi-factor analysis, therefore looking for the disease characteristics and risk factors affecting the survival and prognosis.

RESULTSAmong 118 ET patients enrolled in this study, the survival rate of ET patients for 1, 3, 5 and 10 years were 95.5%,92.6%,89% and 81.6%, respectively. Kaplan-Meier survival curve showed that the age ≥60 years old at diagnosis, cardiovascular risk factors, anamnesis of thrombosis or hemorrhage, anemia(hemoglobin<120 g/L), thrombocythemia (≥1 000×10/L), risk stratification and hydroxyurea or HHT(hemoharringtonine) use in high-risk group were factors affecting the suvival rate, 7 out of those factors influencing survival rate were statistically significant (P<0.05). COX regression analysis showed that independent risk factors affecting survival have not yet been found.

CONCLUSIONET patients display a high survival rate and long survival time, and their conversion risk into the marrow fibrosis or leukemia has been found to be low. The age≥60 years old at diagnosis, cardiovascular risk factors, anamnesis of thrombosis or hemorrhage, anemia and therombocythemia are the risk factors affecting prognosis. The use of hydroxyurea or HHT in high-risk group can improve the prognosis.

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Telomere Length, Expression of MRE11 and Ku80 in Patients with Aplastic Anemia and Their Correlation with Pathogenesis.

Yan WANG ; Rui-Rong XU ; Ying-Jun DU ; Jing-Yi WANG ; Kui LIU ; Wei ZHENG

Journal of Experimental Hematology.2017;25(2):503-509. doi:10.7534/j.issn.1009-2137.2017.02.036

OBJECTIVETo detect the expression levels of MRE11 and Ku80 mRNA, and telomere length in bone marrow mononuclear cells of aplastic anemia(AA) patients, and to explore their correlation with pathogenesis of aplastic anemia.

METHODSBone marrow mononuclear cells were collected from 40 cases of AA and 20 normal controls for detecting mRNA expression of MRE11 and Ku80 and telomere length by using real-time quantitative polymerase chain reaction (qPCR), then MRE11, Ku80 and telomere length were analyzed for their correlation.

RESULTSAs compared with controls, the expression levels of MRE11 and Ku80 in patients with AA were significantly reduced, and the telomere length in patients with AA was obviously shortened, respectively (P<0. 05). The telomere length was significantly shorter in the persons aged ≥45 years in comparison with the AA patients and normal control younger than 45 years old (P<0.05). For the AA patients older than or equal to 45 years and less than 45 years in comparison with the controls at the same age, the telomere length was significantly shorter(P<0.05). The expression levels of MRE11 and Ku80 didn't correlate with telomere length (P>0.05). The mRNA expression level of MRE11 correlated positively and significantly with that of Ku80 (r=0.863, P<0.05).

CONCLUSIONThe change of telomere length may play an important role in the pathogenesis and progression of aplastic anemia. The lower expression of MRE11 and Ku80 may be involved in the pathogenesis of aplastic anemia.

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Analysis of Gene Mutation Types of Thalassemia in Longyan Area of Fujian Province in China.

Qing-Fu DAI ; Xiao-Lu LI ; Yu-Xia WANG ; Chun-Fang CAO

Journal of Experimental Hematology.2017;25(2):498-502. doi:10.7534/j.issn.1009-2137.2017.02.035

OBJECTIVETo explore the type and distribution of thalassemia gene mutation in Longyan area of Fujian province in China, so as to provide a evidence for prenatal diagnosis and to reduce birth defects.

METHODSThe mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and hemoglobin electrophoresis were used for screened the mutation types of thalassemia. Genotyping of the screened positive sample was performed by gap single polymerase chain reaction (gap-PCR) and reverse dot blot hybridization (RDB).

RESULTSOut of 7823 cases of routine positive blood test, 2826 cases were positive (36.12%) by using hemoglobin electrophoresis; 1905 out of 2710 cases were diagnosed as Mediterranean anemia by genetic test, with 24.35% of carrying rate; 1225 cases were positive alpha thalassaemia and the carrying rate was 15.66%, their major genetic types were --/αα,-α/αα,-α/--and -α/αα, with carrying rate of 12.91%, 1.28%, 0.51% and 0.74%, respectively; 632 cases were positive β thalassaemia, with carrying rate of 8.08%, the major genotypes were 654M/N,41-42M/N,17M/N,-28M/N and 27-28M/N and with carrying rate of 3.66%, 2.22%, 0.78%, 0.66% and 0.45%, respectively; 48 cases were diagnosed as both α- and β-thalassemia, with the carrying rate of 0.61%.

CONCLUSIONThe main gene mutation types of α- and β-thalassemia in Longyan area of Fujian Province in China were --/aa and 654 M/N. As thalassemia gene mutation prevalents in Fujian, the screening of thalassemia genotypes for childbearing age woman has great significance for raising population quality.

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Research Advances on Targeted Therapy for Acute Myeloid Leukemia--Review.

Hai-Tao HE ; Hui-Min LI

Journal of Experimental Hematology.2016;24(1):245-249. doi:10.7534/j.issn.1009-2137.2016.01.047

Although the traditional chemotherapy has achieved a certain effect for patients with acute myeloid leukemia (AML), but there are still limitations in terms of improving the rate of complete remission and overcome relapse after remission. The further study found that many cytogenetic molecular and epigenetic abnormalities occurred during the progression of AML, such as abnormal expression of cell surface molecules, mutation, gene aberrant methylation and so on. The drugs targeted at these changes can improve the prognosis for patients, and provide a new way for treating patients with AML. At present, the mostly targeted drugs include monoclonal antibodies CD33-Ab, tyrosine kinase inhibitor, inhibitors of DNA methyltransferases inhibitors and so on. In this review, the progress of targeted therapy in AML treatment is summarized.
Antibodies, Monoclonal ; therapeutic use ; DNA Modification Methylases ; antagonists & inhibitors ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Mutation ; Prognosis ; Protein Kinase Inhibitors ; therapeutic use ; Remission Induction

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Neutrophil CD64 Expression as A Biomarker in the Early Diagnosis of Sepsis in Malignant Hematologic Disease--Review.

Yu-Xi SHANG ; Li-Ru WANG

Journal of Experimental Hematology.2016;24(1):241-244. doi:10.7534/j.issn.1009-2137.2016.01.046

Malignant hematologic disease with sepsis has been characterized by high mortality and difficulty in diagnosis at early stage. A good biomarker may help to improve the accuracy of diagnosis and to reduce the mortality rate. In the early diagnosis of sepsis, neutrophil CD64 expression is a better candidate for biomarker rather than C-reactive proteins. Moreover, neutrophil CD64 expression is also helpful for assessing the severity of infection and prognosis of disease. Unfortunately, there are few studies of neutrophil CD64 expression on the early diagnosis of malignant hematologic diseases. This review focuses on the advantages, limitations, feasibilities and progresses of neutrophil CD64 expression in the early diagnosis of infection in malignant hematologic diseases in this paper.
Biomarkers ; metabolism ; Early Diagnosis ; Hematologic Diseases ; complications ; Humans ; Neutrophils ; metabolism ; Prognosis ; Receptors, IgG ; metabolism ; Sepsis ; complications ; diagnosis

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Research Progress on Long Non-Coding RNA in Hematological Malignancies--Review.

Liu-Yan XIN ; Si-Si ZHONG ; Ai-Fei LIU ; Yi-Jian CHEN

Journal of Experimental Hematology.2016;24(1):237-240. doi:10.7534/j.issn.1009-2137.2016.01.045

Long non-coding RNA (LncRNA) is defined as a class of transcripts more than 200 nucleotides in length and without the protein-coding function. It has been found for years, however, that little is known about the potential role of LncRNA in humans. But recent studies showed that LncRNA can regulate the coding-gene expression and participate in effects of human body. Accumulating evidence demonstrated that LncRNA are involved in cancer incidence, development and progression.With further exploration on the mechanisms of tumors, the relationship between the long non-coding RNA and hematological malignancies increasingly become a hot research. This review focuses on the mechanisms of LncRNA in hematological malignancies.
Hematologic Neoplasms ; genetics ; Humans ; RNA, Long Noncoding ; genetics

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Role of SOX7 in Hematopoietic System Development and Hematological Malignancies--Review.

Wen-Ming WANG ; Jing WANG ; Hong-Mei JING

Journal of Experimental Hematology.2016;24(1):233-236. doi:10.7534/j.issn.1009-2137.2016.01.044

The sex-determining region Y-box 7 (Sox7) is a important member of SOX family containing high mobi- lity group (HMG), mapped to human chromosome 8p23.1. Wnt/β-catenin signaling pathway plays an important role in cell survival, differentiation, self-renewal, proliferation and apoptosis, and is closely related with carcinogenesis. SOX7 gene is likely to be a tumor suppressor gene in MDS and other hematological malignancies. As a negative regulator of the WNT/β-catenin signaling pathway, the function loss of this gene can lead to carcinogenesis. The methylation of SOX7 gene leads to the silence of this gene, resulting in tumorigenesis. The decision of hematopoietic stem cells to self-renew or differentiate is a stochastic process, but SOX7 can promote the differentiation into all blood cell types. This review focuses on the role of SOX7 in hematopoietic system development and hematological malignancies.
DNA Methylation ; Gene Silencing ; Hematologic Neoplasms ; genetics ; metabolism ; Hematopoietic System ; physiopathology ; Humans ; SOXF Transcription Factors ; genetics ; metabolism ; Wnt Signaling Pathway

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Establishment of Method for Detecting Red Blood Cell Osmotic Fragility by Flow Cytometry.

Hong-Yan ZHU ; Qiang MENG ; Hong-Mei OUYAN ; Ting DONG ; Qiong-Yue ZHANG ; You-Quan ZHOU ; Zhu-Xian PING

Journal of Experimental Hematology.2016;24(1):229-232. doi:10.7534/j.issn.1009-2137.2016.01.043

OBJECTIVETo establish a new method for detection of red blood cell osmotic fragility by using flow cytometry.

METHODSThe hypotension salt solution of different concentrations (0.70 ml normal saline+0.3 ml deionized water, 0.60 ml normal saline+0.40 ml deionized water and 0.55 ml normal saline+0.45 ml deionized water) were prepared with normal saline and deionized water, in which the red blood cells were suspended, and the residual red blood cells were detected by flow cytometer.

RESULTSThere was no significant difference in percentage of residual red blood cells between different time points detected by flow cytometer in 3 different hypotonic salt solutions. The percentage of residual red blood cells in B+C+D+E+F+G detected time region was different among 3 NaCl dilution groups. The percentage of residual red blood cells in normal control was lower than that in hemoglobinopathy group. The percentage of residual red blood cells in hereditary spherocytosis (HS) group was obviously lower than that in hemoglobinopathy and normal control groups. The comparison of 3 different dilution concentrations found that the second concentration (0.60 ml normal saline+0.40 ml deionized water) is more suitable to screen HS by FC500 flow cytometer.

CONCLUSIONThe detection of red cell osmotic fragility by using flow cytometry is a simple, rapid, objective and economic way that can be an effective screening method for diagnose the HS.

Erythrocytes ; cytology ; Flow Cytometry ; Humans ; Osmotic Fragility ; Spherocytosis, Hereditary ; physiopathology

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Genetic Indentification of An Individual with A102Bw33 Blood Group Gene Subtypes of ABO Variant.

Feng-Min WANG ; Xu ZHANG ; Jian-Ping LI

Journal of Experimental Hematology.2016;24(1):225-228. doi:10.7534/j.issn.1009-2137.2016.01.042

OBJECTIVETo investigate and indentify the molecular characteristics of a sample serologically identified as ABw subgroup.

METHODSThe individual was confirmed by standard serological techniques. The genotyping and sequencing were performed using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for exon 6 and exon 7 of ABO locus respectively.

RESULTBoth A and B antigen were detected on red blood cells of the proband and anti-B antibody was detected in his serum. PCR-SSP showed that the sample gene was A1B phemotype. DNA sequencing showed 297A/G, 467C/T, 526C/G, 657C/T, 703A/G, 803G/C and 930G/A heterozygote in exon 6 to exon 7. After cloning and sequencing, 2 alleles A102 and Bw33 were obtained. The sequence of Bw33 allele had one nucleotide change (A to C) at position 796 compared with that of B101 allele. The nucleotide in this B allele at site 796 is a "C" characteristic of the A form of the allele.

CONCLUSIONA>C at nt796 of α-1, 3 galactosyltransferase gene can result in Bw33 phenotype with the anti-B antibody in serum.

ABO Blood-Group System ; genetics ; Alleles ; Cloning, Molecular ; Exons ; Genotype ; Heterozygote ; Humans ; Phenotype ; Polymerase Chain Reaction ; Sequence Analysis, DNA

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