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Leukemia-associated immunophenotypes in 415 childhood and adult patients with B lineage acute lymphoblastic leukemia by multiparametric flow cytometry analysis.

Yan-Rong LIU ; Shan-Shan CHEN ; Yan CHANG ; Jia-Yu FU ; Le-Ping ZHANG ; Hui WANG ; Ling-Di LI ; Hong-Hu ZHU ; Gui-Lan LIU ; Dao-Pei LU ; Xiao-Jun HUANG

Journal of Experimental Hematology.2006;14(5):853-857.

To evaluate the significance of FCM in minimal residual disease (MRD) detection, the immunophenotyping and leukemia-associated immunophenotypes (LAIP) of leukemia cells from 273 adult and 142 childhood patients with B lineage acute lymphoblastic leukemia (B-ALL) were detected by four to six antibody combinations of 4-color CD45/SSC gating multiparametric flow cytometry (FCM). The results showed that the B-ALL patients could be classified into 4 subtypes based on different expression CD34 and CD10: subtype I (CD34(+)/CD10(-)), subtype II (CD34(+)/CD10(+)), subtype III (CD34(-)/CD10(+)), subtype IV (CD34(-)/CD10(-)). The LAIP was observed in 100% and 92% patients of subtype I and subtype II, respectively, whereas only 79.2% in subtype III. The incidence of LAIP in total B-ALL cases was 90% by using the antibodies detected in this investigation. There was no significantce different for incidence of LAIP between adult and pediatric patients. LAIP was observed in 77.6% of patients by labeling only CD34/CD10/CD19/CD45 4-color antibody combination. It is concluded that in 90% of childhood and adult B-ALL patients LAIP can be found, which suits MRD detection by multiparameter flow cytometry.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, CD34 ; analysis ; B-Lymphocytes ; immunology ; Burkitt Lymphoma ; classification ; immunology ; pathology ; Cell Lineage ; Female ; Flow Cytometry ; methods ; Humans ; Immunophenotyping ; Male ; Middle Aged ; Neoplasm, Residual ; diagnosis ; Neprilysin ; analysis ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; classification ; immunology ; pathology

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Dendritic cells and transplantation immune tolerance--review.

Hui ZENG ; Guang-Sheng HE ; De-Pei WU

Journal of Experimental Hematology.2006;14(4):849-852.

Dendritic cells (DC) play an important roles in the maintenance of central immune tolerance and peripheral immune tolerance. DC can be involved in formation of autoimmune tolerance by many mechanisms and demonstrate strong plasticity, so that DC become hot issue in the research of transplantation tolerance recently. In this article the DC typing and its role, the indirect pathway of DC-inducing immune tolerance, the F1t3L and apoptotic cell role, the modified DC by genetic engineering and the immune inhibitors were summarized.
Dendritic Cells ; immunology ; Humans ; Immune Tolerance ; Transplantation Tolerance ; immunology

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Role of NK cells in allogeneic hematopoietic stem cell transplantation--review.

Xiao-Hong LI ; Chun-Ji GAO

Journal of Experimental Hematology.2006;14(4):845-848.

After allogeneic hematopoietic stem cell transplantation (allo-HSCT), the donor cells present a profound immunization therapy efficiency. Among these effector cells, allo-reactivity natural killer (NK) cell activation are concerned with the graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect. As known, GVHD is primarily a T-cell-mediated event but not initiated by NK cells. NK cells may significantly enhance GVL immune response by using an integration of activating and inhibitory receptors. Allo-reactivity NK cell infusion after allo-HSCT already transits from experiments to clinic. In this review the background on NK cells, and their clinical roles in Allo-HSCT were summarized.
Graft vs Host Disease ; immunology ; Graft vs Leukemia Effect ; immunology ; Hematopoietic Stem Cell Transplantation ; Humans ; Killer Cells, Natural ; immunology ; Transplantation Immunology

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Progress on the research of microRNA and its functions in lymphoid malignancies--review.

Wei XU ; Jian-Yong LI ; Feng-Xiang LU

Journal of Experimental Hematology.2006;14(4):840-844.

Plant and animal genomes contain an abundance of small genes that produce RNAs of about 22 nucleotides in length, which was dubbed as microRNA (miRNA). These newly found endogenous RNAs may participate in a wide range of genetic regulatory pathways and play an important role in the organism development. This paper reviewed the recent studies and progress on the characteristics, functions and mechanisms of the microRNAs, as well as the advances of research on lymphoid malignancies.
Animals ; Humans ; Leukemia, Lymphoid ; genetics ; Lymphoma ; genetics ; Lymphoproliferative Disorders ; genetics ; MicroRNAs ; biosynthesis ; genetics ; RNA, Small Interfering ; genetics

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Studies on ex vivo expansion of megakaryocytic progenitor and its application--review.

Si LIN ; Sha LIU ; Xin SUN ; Mo YANG

Journal of Experimental Hematology.2006;14(4):835-839.

Application of ex vivo expanded megakaryocytic progenitor cells (MKPC) is a strategy for the treatment of thrombocytopenia after hematopoietic stem cell transplantation. Some growth factors including thrombopoietin (TPO), megakaryocyte growth and development factor (MGDF), interleukin (IL)-1, IL-3, IL-6, IL-11, platelet-derived growth factor (PDGF), and serotonin (5-HT) have been demonstrated to play an important role on the regulation of megakaryocyte/platelet development, the efficient conditions for the expansion of the megakaryocyte (MK) progenitors from hematopoietic stem/progenitor cells were discussed in this review article. TPO alone produced a high proportion of MK progenitors but a low total cell count. The addition of IL-1 beta, IL-3, IL-6 and Flt-3L improved the expansion outcome. The combination of three to five cytokines produced more efficient expansions of hematopoietic stem and MK progenitors. PDGF also enhanced the ex vivo expansion of CD61+ CD41+ cells and CD34+ cells in combination with TPO, IL-1 beta, IL-3, IL-6 and Flt-3L. PDGF is a suitable growth factor to improve the ex vivo expansion of MKPC without promoting their in vitro maturation. More importantly, PDGF also enhanced the engraftment of human stem and progenitor cells in NOD/SCID mice. It has been reported that MKPC can be safely administered to autologous peripheral blood progenitor cell transplant recipients. In short, MKPC can be expanded ex vivo and safely applied to autologous transplant.
Cells, Cultured ; Hematopoietic Stem Cells ; cytology ; Humans ; Megakaryocytes ; cytology ; Peripheral Blood Stem Cell Transplantation ; adverse effects ; methods ; Platelet-Derived Growth Factor ; pharmacology ; Serotonin ; pharmacology ; Thrombocytopenia ; prevention & control ; Thrombopoietin ; pharmacology

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Bone marrow necrosis as an initial manifestation of Philadelphia chromosome and myeloid antigens positive B acute lymphoblastic leukemia--a case report.

Ru-Feng LIN ; Jian-Yong LI ; Hua LU ; Yu-Jie WU ; Hai-Rong QIU ; Bing XIAO ; Jian-Fu ZHANG ; Hui YANG

Journal of Experimental Hematology.2006;14(4):832-834.

Many diseases cause bone marrow necrosis (BMN), especially lymphocytic leukemia. To explore the complexity of the pathogenesis and pathology of BMN and understand the multiplicity of clinical features, a case of Philadelphia chromosome positive (Ph+) B acute lymphoblastic leukemia (ALL) expressing myeloid antigens was reported. The results indicated that the clinical features of this case were complicated and multiplex, the diagnosis was confirmed by using bone marrow smear and biopsy, immunophenotype analysis, conventional cytogenetics and fluorescence in situ hybridization (FISH), the prognosis of patients improved by active treatment for primary disease. In conclusion, the Ph+ B ALL expressing myeloid antigen with BMN is very rare, its diagnosis should be confirmed by using multiple methods, and the active treatments should be performed.
Adult ; Antigens, Neoplasm ; blood ; Bone Marrow ; pathology ; Bone Marrow Diseases ; etiology ; Burkitt Lymphoma ; complications ; genetics ; immunology ; Female ; Humans ; Immunophenotyping ; Necrosis ; etiology ; Philadelphia Chromosome

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Clinical study of unrelated umbilical cord blood transplantation in 32 children patients.

Xi-Feng JIANG ; Bai-Jun SHENG

Journal of Experimental Hematology.2006;14(4):829-831.

To evaluate the clinical efficacy of unrelated umbilical cord blood transplantation (UCBT) on the treatment of children with hematologic malignancies and nonmalignancies, between August 2001 and June 2004, 32 patients were transplanted by using unrelated umbilical cord blood supplied by Shandong Umbilical Cord Blood Bank. Out of them, 13 patients suffered from ALL, 9 from AML, 3 from AA, 3 from HAL, 2 from CML and 2 from NHL. The median age was 8 years (range 2-15), the median weight was 31.5 kg (range 14-55). All patients received ablative conditioning regiment according to the disease and the disease status. Conditioning regiments Cy/TBI were used for 4 patients and Bu/Cy for 21 patients, other for 7 patients. All patients received cyclosporin A and/or methotrexate for GVHD prophylaxis. The mean number of infused nuclear cells were 5.57 (2.16-12.3) x 10(7)/kg, CD34+ cells 1.78 (0.85-5.59) x 10(5). All of UCB units were tested for HLA-A, -B, and DRB1 using low and high resolution techniques. There were HLA-matched in 10, 5/6 in 16 and 4/6 in 6. The results showed that 20 out of 32 patients achieved complete engraftment. Median time of neutrophil > or = 0.5 x 10(9)/L, and platelet > or = 20 x 10(9)/L were 17 (9-38) and 42 (18-102) days respectively. The incidence of aGVHD II-IV and aGVHD III-IV were 35% and 15% respectively. After a median follow-up of 18 months (1.5-28.5), overall survival rate at one year was 59.4%, overall survival rate at two years was 40.6%. It is suggested that UCBT is promising for children patients who is lack of matched bone marrow donors.
Adolescent ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Child ; Child, Preschool ; Cord Blood Stem Cell Transplantation ; methods ; Cyclosporine ; administration & dosage ; Graft vs Host Disease ; prevention & control ; Hematologic Neoplasms ; mortality ; therapy ; Humans ; Methotrexate ; administration & dosage ; Survival Rate ; Transplantation Conditioning

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Influence of raising oxygen content on function of platelet concentrate during preservation.

Tong ZHAN ; Jian-Yu XIAO ; Jing TAO ; Xi-Feng MIAO ; Yan-Cun LIU ; Rong-Cai TANG

Journal of Experimental Hematology.2006;14(4):826-828.

To explore the influence of raising oxygen (dissolved oxygen) content on function of platelet concentrate, the platelet concentrate was prepared by a CS-3000 plus blood cell separator. Experiments were divided into 2 groups: test group and control group. After raising oxygen content in platelet plasma under sterile operation, the platelet samples of two groups were preserved in oscillator with horizontal oscillation at 22 +/- 2 degrees C. The platelet count, platelet aggregation rate, lactic acid content and CD62p expression level of platelet were detected on 0, 1, 2, 3, 4, 5 days of platelet preservation. The results showed that the platelet count and platelet aggregation rate decreased with prolongation of preserved time, while the lactic acid content and CD62p expression level of platelet increased gradually. Compared with control group, there were significant differences in aggregation rate of platelet preserved for 2-3 days, and in CD62p expression level of platelet preserved for 1-3 days, while significant difference was found in lactic acid content of platelet preserved for 1-3 days. It is concluded that raising content of oxygen in platelet plasma can provide more oxygen to compensate oxygen supply deficiency for platelet metabolism and improve the efficiency of platelet oxygenic metabolism and the quality of platelet during preservation.
Blood Platelets ; drug effects ; physiology ; Blood Preservation ; methods ; Humans ; Lactic Acid ; metabolism ; Oxygen ; pharmacology ; Platelet Aggregation ; drug effects ; Platelet Count ; Platelet Function Tests

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Polymorphism of D2S1338 and D19S433 loci in Southern Chinese Han nationality population and its application.

Xuan ZHANG ; Zhi-Hui DENG ; Zhen LI ; Guo-Guang WU

Journal of Experimental Hematology.2006;14(4):822-825.

This study was aimed to investigate the polymorphism of D2S1338 and D19S433 loci in Southern Chinese Han nationality population, and to evaluate its forensic application. The DNA was extracted by chromatography on che-lex-100. Fifteen STR loci (D2S1338, D19S433 etc.) were amplified by Identifiler kit and analyzed by 3100 genetic analyzer. The softwares of analysis were GeneScan 3.0 and Genetype 3.7. The results indicated that the allele frequencies of H, DP, PIC, PE of D2S1338 and D19S433 loci were obtained. The allele frequency of samples were in Hardy-Weinberg equilibrium by chi2 test. No mutation was found on D2S1338 and D19S433 loci in 370 cases of meiosis. It is concluded that the D2S1338 and D19S433 loci are high polymorphic and their mutation rates in Southern Chinese Han nationality population are low. Their good distribution is suitable for forensic individual identification and paternity testing.
Alleles ; Asian Continental Ancestry Group ; genetics ; China ; ethnology ; DNA Fingerprinting ; methods ; Forensic Medicine ; methods ; Gene Frequency ; Genetic Variation ; Humans ; Polymorphism, Genetic ; Tandem Repeat Sequences

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Animal trials for mPEG-modified red blood cells.

Yan QIU ; Ying-Xia TAN ; Li-Li LI ; Su-Bo LI ; Qiu-Shuang LÜ ; Yi ZHA ; Yang-Pei ZHANG

Journal of Experimental Hematology.2006;14(4):816-821.

This study was aimed to investigate the survival rate and difference of transfused modified and unmodified RBC at 24 hours. The modified and unmodified RBC from mice, monkey, pig and human were labeled by using FITC, then these blood RBCs were transfused to homogeneous and heterogeneous animals. The result showed that 24 hour survival rate of unmodified mice RBC transfused to mice was 74%, while survival rate of 2.0 mmol/L mPEG-SPA modified mice RBC transfused to mice was 45%, difference between them was significant. The 24 hour survived rate of unmodified human RBC transfused to mice was 8%, while 24 hours survival rate of 2.0 mmol/L mPEG-SPA modified human RBC transfused to mice was 5% without statistical difference. The 24 hour survived rate of homogeneous transfusion of modified monkey RBC was 90%, while survival rate of modified human and pig RBC was zero on 24 hours after transfusion to monkey. It is concluded that RBC labeling methods and mice species are unrelated to 24 hours survival rate, but mPEG variety and concentration are related to mouse RBC life-span. It is incredible to use mouse RBC homogeneous transfusion result instead of human RBC to evaluate longevity and safety of modified human RBC. But modified human RBC transfused to mice can be a model to evaluate longevity of modified human RBC. It is very difficult to get the result about modified RBC life span by RBC transfusion among great heterogeneous mammal animals. So evaluation in large mammal animal models needs to be further studied.
Animals ; Cell Survival ; drug effects ; Erythrocyte Transfusion ; methods ; Humans ; Macaca mulatta ; Male ; Mice ; Polyethylene Glycols ; pharmacology ; Swine

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