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Antibiotic Resistance of Bacteria Isolated from Bile Specimens

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2000;29(5):469-470，479.

To investigate the antibiotic resistance of bacteria isolated from bile, the bile from the surgical in-patients with bile duct infection from June 1995 to December 1998 was cultured and the distribution and antibiotic resistance of the isolates (not including anaeobes) were analyzed. The results showed that the cultured positive rate in the specimens was 69. 76 % (143/205). Among the isolates, most of them were E. coli and P. aeruginosa (31. 74 % and 18. 56 %, respectively), while Enterobacter, K. pneumonia and Enterococci were also commonly seen. All the isolates were resistant more or less to the commonly used antibiotics.

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Protection of Intrahepatic Injection of Liposome-mediated VEGF Plasmid against Ischemia-reperfusion Liver Injury

Xudong XU ; Yaqun WU ; Quan SUN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(5):590-593,611. doi:10.3870/j.issn.1672-0741.2009.05.006

Objective To explore the effect of intrahepatic injection of liposome-mediated VEGF plasmid on ischemia-reper-fusion liver injury and its mechanism. Methods Rabbits were randomly divided into normal group, ischemia-reperfusion group and recombinant VEGF therapy group( liposome-mediated transfer of VEGF plasmid into liver via portal vein 20 min before ischemia of liver). The model of liver ischemia-reperfusion injury was established. Liver function and the activity of SOD.XO in blood were determined at the 0,2nd,6th,12th,and 24th h after operation. RT-PCR technique was applied to detect the expression level of Fas mRNA in liver tissues of every group,and flow cytometry was used to measure cell apoptosis rate at the 6th h after operation. At the 24th h after operation,all rabbits were killed and liver tissues of ischemia were taken to make pathological sections for observing the morphology and microstructure under the light microscopy and electron microscopy. ResuJts The level of ALT in recombinant VEGF therapy group was markedly reduced as compared with ischemia-reperfusion group at the 6th,12th,and 24th h after operation( P<0. 05). The activity of SOD in recombinant VEGF therapy group was significantly higher than in ischemia-reperfusion group at the 6th, 12th,and 24th h after operation. The activity of XO in recombinant VEGF therapy group was significantly lower than that in ischemia-reperfusion group at the 6th,12th,and 24th h after operation(P< 0. 05 or P<0. 01). In addition,there was significant difference in the expression of Fas mRNA and cell apoptosis rate between recombinant VEGF therapy group and ischemia-reperfusion group(P<0. 01). The injury of hepatocytes in recombinant VEGF therapy group was significantly alleviated as compared with that in ischemia-reperfusion group under the light microscopy and e-lectron microscopy. Conclusion Liposome-mediated transfer of VEGF plasmid into liver before ischemia of liver can obviously protect hepatocytes by increasing anti-oxidative ability, decreasing the expression of Fas mRNA, and finally inhibiting hepato-cyte apoptosis.

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Impact of NF-kappaB Activation on TNF-α and IL-1β Expression in Myocardial Ischemia/Reperfusion Injury

Tucheng SUN ; Xionggang JIANG ; Kailun ZHANG ; Zongquan SUN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(6):752-755,759. doi:10.3870/j.issn.1672-0741.2009.06.008

Objective To study the impact of NF-kappaB activation on TNF-a and IL-1β expression in myocardial ischemia/reperfusion(I/R) injury.Methods Sixty-five Sprague-Dawley rats were randomly divided into three groups:sham,n=5;I/R:30 min of myocardial ischemia followed by 0,15,30,60,120.240 min of reperfusion,n=5 per subgroup;I/R+PDTC:PDTC(15 mg/kg)was given before ischemia,and the time points were the same as those in I/R group.TNF-a and IL-1β mRNA expression was detected by RT-PCR,activity of NF-KB was measured by electrophoretic mobility shift assay (EMSA),and MDA level in myocardium was assayed by TBA method.Results The expression of TNF-a and IL-1β was increased before reperfusion,reached their peak at the time point of reperfusion 30.60 min respectively.and remained high level at the 2nd h after reperfusion.NF-KB was activated 15 min after reperfusion,reached its peak at the first h after reperfusion.In I/R+PDTC group,NF-κB activation was blocked by PDTC.As compared with I/R group,the expression levels of TNF-a and IL-1β were decreased to varying degrees at each time point.and the content of MDA was also reduced in I/R+PDTC group.Conclusion NF-KB activation could play a pivotabrole in the expression of cytokine.Inhibition of NF-κB signal pathway might be a potential therapeutic strategy in reperfusion injury.

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Analysis of the Positive Results of Fungus Culture in 95 Cases of Prostatitis

Mufen WAN ; Yingling CHEN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):277-278,281.

Fungus infection of the patients with chronic prostatitis was studied and the applied value of modified Sabourand's liquid medium in fungus culture explored. The causative fungus in expressed prospapic secrepion (EPS) of chronic prostatitis patients were examined by improved Sabouraud's liquid medium and Sabouraud's glucose agar medium. The fungus species were identified. The results showed that in 1138 cases positive for fungus，the specimens of 95 cases (8.35 %) were cultured in the improved Sabouraud's liquid medium and those of 30 cases (2.64 %) in Sabouraud's glucose agar medium. In the 95 cases，63 strains were Candida albicans (63.3 %)，15 Candida tropicalis (15.8 %)，and 17 other Candida (17.9 %). It was concluded that: (1) The relationship between chronic prostatitis and fungus infection can not be ignored. The attention should be paid to the possibility of fungus infection for those with chronic postatitis who were treated with long-time antibiotics but the curative effects were not satisfactory;(2) In Candidal prostatitis，Candida albicans was a common causative fungus;(3) Application of the improved Sabouraud's liquid medium could increase the positive rate of Candida.

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The Expression and Significance of Interleukin-6 in Prostate Cancer

Shixin BAO ; Weimin YANG ; Zhangqun YE

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(5):696-699,702. doi:10.3870/j.issn.1672-0741.2009.05.031

Objective To study the role of interleukin-6(IL-6)in the pathogenesis of prostate cancer and its clinical significance. Methods Immunohistochemistry and RT-PCR were used to detect the expression of IL-6 protein and mRNA in frozen prostatic adenocarcinoma,adjacent benign prostatic tissue,and prostate cancer cell lines PC-3 and LNCaP. The serum levels of IL-6 in patients with prostate cancer and healthy controls,and the supernatants of prostate cancer cell cultures were measured by using ELISA. Results The IL-6 protein levels in prostate cancer tissue and PC-3 cells were significantly higher than those in adjacent benign prostatic tissue and LNCaP cells. The serum IL-6 levels in the patients with prostate cancer were markedly higher than those in the healthy controls. The IL-6 levels in supernatants in PC-3 cells were notably higher than those in the LNCaP cells. Conclusion The IL-6 gene may act as an important regulator in prostate cancer progression and may be one of the causes of prostate cancer conversion from an initially androgen-dependent state into an androgen-independent state.

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Determination of Tetramethylpyrazine in Ligusticum Chuanxiong Hort by RP-HPLC Coupled with Countercurrent Extraction

Xinguo SUN ; Tao WANG ; Jingshen ZHU

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):209-210.

An analytical method for determination of tetramethylpyrazine (TMP) in ligusticum chuanxiong hort by RP-HPLC coupled with countercurrent extraction used for the preconcentration of trace TMP was established. The column was packed with Zorbax ODS (250 mm×4 mm，5 μm). The mobile phase consisted of methanol water (1 % acetic acid)(35∶65)，the flow rate was 0.8 ml/min and detection was performed at 300 nm. The calibration curves showed good linearity over the range of 0.41 μg/ml to 4.1 μg/ml，γ＝0.9993，the recoveries were 92.3 %. The method was simple，fast，sensitive and accurate and could be applied to the analysis of ligusticum chuanxiong hort. The results showed that the content of TMP in ligusticum chuanxionghort were different with different sources.

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Clinical Implication of Microvessel Density and Vascular Endothelial Growth Factor Expression in Breast Cancer

Hongge WU ; Lüguang LIU

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(2):148-150.

The clinical implication of microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression in breast cancer and the relationship between them were studied. Specimens of 53 cases of breast cancer were examined for MVD in tumor and VEGF expression by using immunohistochemical staining. The results showed that the mean MVD in node-positive breast cancers and in the patients with postoperative distant metastasis was higher than that in node-negative breast cancers and those without metastasis, respectively (both P<0.01). The expression rate of VEGF in breast cancer was 67.92 % and was closely relative to MVD. It was suggested that MVD was closely correlated with metastasis of the tumors and might be a useful prognostic indicator for breast cancer. VEGF might be an important factor responsible for the induction of angiogenesis in breast cancer.

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Studies on the Confirmation of the Structure of Triplidine Hydrochloride

Suyue LI ; Jingshen ZHU

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(2):142-144.

The chemical structure of Triplidine Hydroloride was confirmed by using EA, IR, UV-Vis, 1NMR, 13NMR, MS after it was synthesized. It was concluded that the synthetic Triplidine Hydrochloride was the same as the standard.

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Clinical Characters of Rhegmatogenous Retinal Detachment

Jinghua SUN ; Hong YANG ; Wei XUE

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):292-293.

The clinical data of 109 patients with rhegmatogenous retinal detachment were retrospectively analyzed and the relationships among retinal detachment，refraction and the shapes of hole in different age groups discussed. The results showed that myopia was more commonly seen in the patients less than 40 years old and the occurrence of myopia was gradually decreased in the patients over 40 years old. Round hole was more commonly seen in the patients less than 40 years old and horseshoe-shaped tears were more often found in the age group of over 40 years old.

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The Clinical Use of Double-paper Synergy Test in the Detection of Bacteria Producing Extended-spectrum β-lactamase

Wei XIONG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):285-288.

The optimal condition of double-paper synergy test in the detection of bacteria producing extended-spectrum β-lactamase (ESBLS) was studied. In 244 isolates of Enteric bacilli，Klebsiella pneumonia，Enteric cloacae，there were 49 ESBLS-producing strains with the positive rate being 19.67 %. Five ESBLS-producing strains were detected in Pseudomonas maltophilla. One ESBLS-producing strains was detected in Pseudomonas aeruginose，Enteric aerogenes and Citrobacter，respectively. The optimal substrates of double-paper synergy test were cefotaxime，aztreonam and cefepime，and the interspace was 25 mm. This method was still a simple，economical and appropriate method in the detection of ESBLS-producing isolates.

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