Journal Detail Information

1

Cite

Share

Protection of Intrahepatic Injection of Liposome-mediated VEGF Plasmid against Ischemia-reperfusion Liver Injury

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(5):590-593,611. doi:10.3870/j.issn.1672-0741.2009.05.006

Objective To explore the effect of intrahepatic injection of liposome-mediated VEGF plasmid on ischemia-reper-fusion liver injury and its mechanism. Methods Rabbits were randomly divided into normal group, ischemia-reperfusion group and recombinant VEGF therapy group( liposome-mediated transfer of VEGF plasmid into liver via portal vein 20 min before ischemia of liver). The model of liver ischemia-reperfusion injury was established. Liver function and the activity of SOD.XO in blood were determined at the 0,2nd,6th,12th,and 24th h after operation. RT-PCR technique was applied to detect the expression level of Fas mRNA in liver tissues of every group,and flow cytometry was used to measure cell apoptosis rate at the 6th h after operation. At the 24th h after operation,all rabbits were killed and liver tissues of ischemia were taken to make pathological sections for observing the morphology and microstructure under the light microscopy and electron microscopy. ResuJts The level of ALT in recombinant VEGF therapy group was markedly reduced as compared with ischemia-reperfusion group at the 6th,12th,and 24th h after operation( P<0. 05). The activity of SOD in recombinant VEGF therapy group was significantly higher than in ischemia-reperfusion group at the 6th, 12th,and 24th h after operation. The activity of XO in recombinant VEGF therapy group was significantly lower than that in ischemia-reperfusion group at the 6th,12th,and 24th h after operation(P< 0. 05 or P<0. 01). In addition,there was significant difference in the expression of Fas mRNA and cell apoptosis rate between recombinant VEGF therapy group and ischemia-reperfusion group(P<0. 01). The injury of hepatocytes in recombinant VEGF therapy group was significantly alleviated as compared with that in ischemia-reperfusion group under the light microscopy and e-lectron microscopy. Conclusion Liposome-mediated transfer of VEGF plasmid into liver before ischemia of liver can obviously protect hepatocytes by increasing anti-oxidative ability, decreasing the expression of Fas mRNA, and finally inhibiting hepato-cyte apoptosis.

2

Cite

Share

Antibiotic Resistance of Bacteria Isolated from Bile Specimens

Xuhui ZHU ; Ru CHEN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2000;29(5):469-470，479.

To investigate the antibiotic resistance of bacteria isolated from bile, the bile from the surgical in-patients with bile duct infection from June 1995 to December 1998 was cultured and the distribution and antibiotic resistance of the isolates (not including anaeobes) were analyzed. The results showed that the cultured positive rate in the specimens was 69. 76 % (143/205). Among the isolates, most of them were E. coli and P. aeruginosa (31. 74 % and 18. 56 %, respectively), while Enterobacter, K. pneumonia and Enterococci were also commonly seen. All the isolates were resistant more or less to the commonly used antibiotics.

3

Cite

Share

Impact of NF-kappaB Activation on TNF-α and IL-1β Expression in Myocardial Ischemia/Reperfusion Injury

Tucheng SUN ; Xionggang JIANG ; Kailun ZHANG ; Zongquan SUN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(6):752-755,759. doi:10.3870/j.issn.1672-0741.2009.06.008

Objective To study the impact of NF-kappaB activation on TNF-a and IL-1β expression in myocardial ischemia/reperfusion(I/R) injury.Methods Sixty-five Sprague-Dawley rats were randomly divided into three groups:sham,n=5;I/R:30 min of myocardial ischemia followed by 0,15,30,60,120.240 min of reperfusion,n=5 per subgroup;I/R+PDTC:PDTC(15 mg/kg)was given before ischemia,and the time points were the same as those in I/R group.TNF-a and IL-1β mRNA expression was detected by RT-PCR,activity of NF-KB was measured by electrophoretic mobility shift assay (EMSA),and MDA level in myocardium was assayed by TBA method.Results The expression of TNF-a and IL-1β was increased before reperfusion,reached their peak at the time point of reperfusion 30.60 min respectively.and remained high level at the 2nd h after reperfusion.NF-KB was activated 15 min after reperfusion,reached its peak at the first h after reperfusion.In I/R+PDTC group,NF-κB activation was blocked by PDTC.As compared with I/R group,the expression levels of TNF-a and IL-1β were decreased to varying degrees at each time point.and the content of MDA was also reduced in I/R+PDTC group.Conclusion NF-KB activation could play a pivotabrole in the expression of cytokine.Inhibition of NF-κB signal pathway might be a potential therapeutic strategy in reperfusion injury.

4

Cite

Share

Analysis of the Positive Results of Fungus Culture in 95 Cases of Prostatitis

Mufen WAN ; Yingling CHEN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):277-278,281.

Fungus infection of the patients with chronic prostatitis was studied and the applied value of modified Sabourand's liquid medium in fungus culture explored. The causative fungus in expressed prospapic secrepion (EPS) of chronic prostatitis patients were examined by improved Sabouraud's liquid medium and Sabouraud's glucose agar medium. The fungus species were identified. The results showed that in 1138 cases positive for fungus，the specimens of 95 cases (8.35 %) were cultured in the improved Sabouraud's liquid medium and those of 30 cases (2.64 %) in Sabouraud's glucose agar medium. In the 95 cases，63 strains were Candida albicans (63.3 %)，15 Candida tropicalis (15.8 %)，and 17 other Candida (17.9 %). It was concluded that: (1) The relationship between chronic prostatitis and fungus infection can not be ignored. The attention should be paid to the possibility of fungus infection for those with chronic postatitis who were treated with long-time antibiotics but the curative effects were not satisfactory;(2) In Candidal prostatitis，Candida albicans was a common causative fungus;(3) Application of the improved Sabouraud's liquid medium could increase the positive rate of Candida.

5

Cite

Share

The Expression and Significance of Interleukin-6 in Prostate Cancer

Shixin BAO ; Weimin YANG ; Zhangqun YE

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2009;38(5):696-699,702. doi:10.3870/j.issn.1672-0741.2009.05.031

Objective To study the role of interleukin-6(IL-6)in the pathogenesis of prostate cancer and its clinical significance. Methods Immunohistochemistry and RT-PCR were used to detect the expression of IL-6 protein and mRNA in frozen prostatic adenocarcinoma,adjacent benign prostatic tissue,and prostate cancer cell lines PC-3 and LNCaP. The serum levels of IL-6 in patients with prostate cancer and healthy controls,and the supernatants of prostate cancer cell cultures were measured by using ELISA. Results The IL-6 protein levels in prostate cancer tissue and PC-3 cells were significantly higher than those in adjacent benign prostatic tissue and LNCaP cells. The serum IL-6 levels in the patients with prostate cancer were markedly higher than those in the healthy controls. The IL-6 levels in supernatants in PC-3 cells were notably higher than those in the LNCaP cells. Conclusion The IL-6 gene may act as an important regulator in prostate cancer progression and may be one of the causes of prostate cancer conversion from an initially androgen-dependent state into an androgen-independent state.

6

Cite

Share

Detection of Mesentery Circumferential Margin Invasion of Middle and Low Rectal Cancer with Large Slices and CK20

Bin YU ; Yueming YU ; Wenhe ZHAO

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2010;39(1):133-135. doi:10.3870/j.issn.1672-0741.2010.01.034

Objective To study the rules of circumferential margin invasion(CMI)in middle and(or)lower rectal cancers by pathological large slices stained with HE and labeled with CK20,and provide a pathological basis for clinical therapy of rectal cancer.Methods Forty-one patients with middle or low rectal cancer were randomly selected in 8 months.The surgically resected specimens were detected after they were made into the pathological large slices stained with HE and labeled with CK20.Results The mesentery CMI positive rate in the pathological slices with HE staining and CK20 labeling was 21.95%,and 29.27% respectively.The mesentery CMI positive rate in moderately-and well-differentiated group was lower than in poorly-differentiated group(P＜0.05).Moreover,the mesentery CMI rate was higher in the specimens with lower edge less than 5 cm away from the dentate line than that in those with lower edge more than 5 cm away from the dentate line(P＜0.05).There was no significant correlation between CMI with the factors such as gender,age,pathological general types,tumor infiltration depth,lymph node metastasis,and surgical procedures(all P＞0.05).Conclusion There were risk factors for the CMI such as low location,poor differentiation and so on.The CMI patients should be treated with standardized adjunctive therapy after operation.

7

Cite

Share

Completely Thoracoscopic Resection of Benign Neurogenic Tumors on Apical Chest

Qinzi XU ; Min ZHU ; Xiangning FU ; Yingxiong SHANG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2010;39(1):130-132. doi:10.3870/j.issn.1672-0741.2010.01.033

Objective To discuss the feasibility,safety,technical points and clinical effects of completely thoracoscopic resection of benign neurogenic tumors on apical chest.Methods From January 2004 to June 2009,11 patients underwent surgical resection of benign neurogenic tumours on apical chest.A complete thoracoscopy was used in 5 cases,and the remaining 6 cases received traditional open thoracotomy.By analysis on the clinical symptoms,tumor types,complications,operative time,blood loss and drainage time after operation,the advantages and disadvantages of complete thoracoscopy were compared to traditional open thoracotomy for resection of benign neurogenic tumors on apical chest.Results There was one patient in each group that suffered from light transient Horner's syndrome,who recovered spontaneously.The group of complete thoracoscopy was superior to the group of traditional open thoracotomy in operative time,blood loss during the operation,drainage time and postoperative hospital stay.Conclusion For benign neurogenic tumors on apical chest,a resection with complete thoracoscopy is as safe and effective as the traditional open thoracotomy,and the former is characterized by less operative trauma and quicker recovery.

8

Cite

Share

Combined Use of Traditional Chinese and Western Medicine for Chronic Pelvic Inflammatory Diseases: A Meta-analysis

Minghui FAN ; Fengli ZHANG ; Ye REN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2010;39(1):127-129. doi:10.3870/j.issn.1672-0741.2010.01.032

Objective To evaluate the clinical effectiveness of combined use of traditional Chinese and Western medicine for chronic pelvic inflammatory diseases.Methods By searching in the major databases of CNKI,ISI Web of Knowledge,VIP Information and Pubmed,we collected data of randomized controlled trials pertaining to combined use of traditional Chinese and Western medicine in the treatment of chronic pelvic inflammatory diseases.Results Twenty-five randomized controlled trials involving totally 3081 patients were collected according to the inclusion criteria,and meta-analysis of the data indicated that combined use of traditional Chinese and Western medicine can be of great value in the management of chronic pelvic inflammatory diseases in comparison with exclusive use of Western medicine [combined odds ratio(OR)was 3.20 with 95% confidence interval(CI)(2.74,3.73),Z=14.85,P＜0.01].Conclusion The clinical evidence derived from the analysis suggests that the combination of traditional Chinese and Western medicine for chronic pelvic inflammatory diseases can be effective with good security.

9

Cite

Share

Enhancement of Drug Sensitivity to 5-FU in Human Colorectal Cancer Cell Caco-2 by EGF Stimulation

Lin YE ; Guobin WANG ; Kaixiong TAO

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2010;39(1):120-123. doi:10.3870/j.issn.1672-0741.2010.01.030

Objective By observing the effectiveness of epidermal growth factor(EGF)combined with 5-flauouracil(5-FU)in the treatment of human colorectal cancer,to explore the anti-tumor effects of EGF.Methods MTT assay was used to evaluate the effects of EGF and 5-FU alone or combined use on the growth of tumor cells.The effects of EGF on PCNA expression were detected by Western blot,and flow cytometry was done to measure the changes in cell cycle.Results EGF could induce proliferation of tumor cells.With the increase in concentration of EGF,the number of cells in G0 phase was significantly reduced,and the expression of PCNA obviously increased.There was significant difference between 100 or 1 000 ng/mL EGF groups and control group(P＜0.05).The combined use of EGF and 5-FU could more significantly inhibited the growth of tumor cells than 5-FU alone(P＜0.05).Conclusion EGF can enhance the sensitivity of colorectal cancer cell Caco-2 to 5-FU,which might be related to the induction of proliferation of quiescent colorectal cancer cells.

10

Cite

Share

Chitosan Promoting the Growth of in vitro Cultured Retinal Nerve Cells of SD Baby Rats

Hong YANG ; Yan ZHAO

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2010;39(1):116-119. doi:10.3870/j.issn.1672-0741.2010.01.029

Objective To study the effect of chitosan on the growth of the in vitro cultured retinal nerve cells. Methods The retinal nerve cells of SD baby rats were cultured in vitro. The effects of chitosan at 0.065%,1.25%,2.5%,5%,10%,15% on the growth of the retinal nerve cells were measured by MTT method. The blank control group was created. Results 1.25%,2.5%,5%,and 10% chitosan could obviously promote the growth of retinal nerve cells,and 10% chitosan was the optimal concentration for the growth of nerve cells. Conclusion Chitosan can effectively promote the growth of retinal nerve cells,and it is necessary to undertake the further study of Chitosan.

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share