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Clinical Characters of Rhegmatogenous Retinal Detachment

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):292-293.

The clinical data of 109 patients with rhegmatogenous retinal detachment were retrospectively analyzed and the relationships among retinal detachment，refraction and the shapes of hole in different age groups discussed. The results showed that myopia was more commonly seen in the patients less than 40 years old and the occurrence of myopia was gradually decreased in the patients over 40 years old. Round hole was more commonly seen in the patients less than 40 years old and horseshoe-shaped tears were more often found in the age group of over 40 years old.

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The Clinical Use of Double-paper Synergy Test in the Detection of Bacteria Producing Extended-spectrum β-lactamase

Wei XIONG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):285-288.

The optimal condition of double-paper synergy test in the detection of bacteria producing extended-spectrum β-lactamase (ESBLS) was studied. In 244 isolates of Enteric bacilli，Klebsiella pneumonia，Enteric cloacae，there were 49 ESBLS-producing strains with the positive rate being 19.67 %. Five ESBLS-producing strains were detected in Pseudomonas maltophilla. One ESBLS-producing strains was detected in Pseudomonas aeruginose，Enteric aerogenes and Citrobacter，respectively. The optimal substrates of double-paper synergy test were cefotaxime，aztreonam and cefepime，and the interspace was 25 mm. This method was still a simple，economical and appropriate method in the detection of ESBLS-producing isolates.

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Study on the Cardiac Damage in the Patients with Polymyositis/Dermatomyositis

Shaoxian HU ; Peigen HE

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):235-236.

To investigate the significance of cardiac damage in the patients with polymyositis (PM)/dermatomyositis (DM)，45 cases of PM/DM in our hospital from 1992 to 1998 were retrospectively analyzed in the aspects of the clinical data，muscle zymogram，X-ray plain films，electrocardiograph，ultrasonic cardiogram. It was found that cardiac damage (51.1 %) occurred in 23 out of 45 cases of PM/DM. Pericardium，cardiac muscle，endocardium and coronary artery were involved. Clinical manifestations were various. Among the cases of cardiac damage，3 died of heart failure. It was indicated that cardiac damage is the most common complication and also one of the common death causes of PM/DM.

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Experimental Study of Lipid-Regulating Capsule and Simvastatin for the Dislipidemic SD Rats

Xiaomei GUO ; Shouhua SUN ; Zhengkun QIAN

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):232-234.

The role of lipid-regulating capsule in the regulation of experimental dislipidemia was studied in SD rats. The SD rats were divided into 6 groups: group A (normal control)，group B to F (experimental hyperlipidemia). The rats in the groups C，D and E received the capsule in a daily dose of 2.5 g，5 g and 10 g/kg respectively for 3 weeks，while the rats in the group F received Simvastatin in a daily dose of 7.5 mg/kg for 3 weeks. By comparison with the group B after 3 weeks，the levels of plasma total cholesterol (TC) and low density lipoprotein (LDL-C) were very significantly decreased in the groups C，D，E and F(P<0.01)，the value of plasma total triglyceride (TG) was very significantly decreased in the groups D，E and F (<0.01)，and the level of high density lipoprotein was significantly increased in the groups C，D and E (P<0.05)，but very significantly increased in the group F (P<0.01). It was suggested that the therapeutic efficiency of the lipid-regulating capsule in low，middle and high dosage was the same as that in high dose of simvastatin for the SD rats with high plasma TC and LDL-C，and middle and high doses of the capsule had the same effect of simvastatin on the plasma TG.

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Mutations of P16 Gene in 8 Human Malignant Lymphoma Cell Lines

Nianlan YANG ; Yan CHEN ; Chongyu LI

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):229-231.

In order to determine the relationship between P16 and the tumorigenesis of malignant lymphoma，silver-stained PCR-SSCP analysis and direct sequencing were used to evaluate the alterations of P16 gene in 8 human malignant lymphoma cell lines. A homolygous loss was found in SU-DHL-9 while mutations of P16 exon 2 in SU-DHL-1 and Daudi. Sequencing of the PCR-product showed three point mutations in SU-DHL-1，which were GAC to AAC in codon 72，GOT to ACT in codon 77，and a insertion of C between codon 82 and 83，respectively. This study suggested the mutations of P16 gene are highly frequent in B-cell lymphoma cell lines and may play an important role in its tumorigenesis.

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Effect of Erigeron Breviscapini on Chronic Hypoxic Pulmonary Hypertension and Serum Nitric Oxide and Endothelin-1 in Rats

Yipeng DING ; Yongjian XU ; Zhenxiang ZHANG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):224-226.

To study the effect of Erigeron breviscapini on chronic hypoxic pulmonary hypertension and mechanism，the hypoxic rats were injected intraperitoneally with Erigeron breviscapini. The mPAP，the content of serum nitric oxide (NO) and endothelin-1 (ET-1) were measured 5，14 and 28 days after intraperitoneal injection of Erigeron breviscapini in hypoxia respectively，and were compared with those in the single hypoxic group. The results showed that the contents of serum mPAP and ET-1 were higher，and serum NO was lower in single hypoxic group than in the normal rats (both P<0.01). The contents of serum mPAP and ET-1 were lower and NO was higher in the Erigeron breviscapini-treated group than in the single hypoxic group (P<0.01). It was suggested that Erigeron breviscapini could reduce the hypoxic pulmonary hypertension by increasing NO and decreasing ET-1 of serum. Erigeron breviscapini could be used to treat hypoxic pulmonary hypertension.

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Experimental Study on the Effects of Immediate Administration of Inderal on Postinfarction Left Ventricular Remodeling of the Rabbit

Peng ZHENG ; Zhaoming HU

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(3):221-223.

The effects of immediate administration of inderal following acute myocardial infarction on left ventricular remodeling were studied in acute myocardial infarction model of the rabbit. The results showed that there was no significant difference in the infarct size and expansion index between the infarct-inderal group and the infarct-placebo group. As compared with sham surgery-inderal group，infarct-inderal group had a higher ventricular weight，left ventricular volume index and left ventricular wall stress ［(2.65±0.17) g/kg vs (2.32±0.11) g/kg，P<0.05;(2.22±0.23) ml/kg vs (1.70±0.12) ml/kg，P<0.001;(0.84±0.07) vs (0.75±0.052)，P<0.05，respectively］，but there was no significant difference between the infarct-inderal group and infarct-placebo group. Heart rate in the infarct-inderal group was significantly decreased as compared with that of infarct-placebo group ［(153±6.36) min－1 vs (225±4.08) min－1，P<0.001］，but there was no significant difference in left ventricular end-diastolic pressure，+dp/dt max and cardiac index between the two groups. It was suggested that immediate administration of inderal postinfarction had no preventive effect on left ventricular remodeling.

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Experimental Study of Red Blood Cells after Cold Storage at -80℃

Zhongqing YU ; Lihua HU ; Shenzong RAO

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(2):175-177.

The changes in shape, structure and function of red blood cells (RBC) after cold storage were observed by using slow cold storage technique. Before and after cold storage, RBC mean corpuscular volume (MCV), RBC fragility (RF), whole blood viscosity (WBV), RBC deformity (RBCD), LFU, SatO2, hematocrit (HCT), RBC recovery rate (RBCRR) and RBC immune function were measured. The result showed that after resuscitation, RBC had vital resistance to hypotonic solution. There was no significant difference in MCV, RBCD and LFU (P>0.05). HCT and WBV were decreased and RBCRR reached 84.1 %(all P<0.01). SatO2 was increased from 30.5 % to 98.2 %. RBC immune function showed no significant difference (P>0.05). It was suggested that quality of RBC didn't change after cold storage and RBC could be cryopreserved for a long time.

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Detection of EGF in Tears from Patients with Herpes Simplex Virus Keratitis

Zhulin HU ; Changsong ZHAO ; Xiaonong ZHANG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(2):173-174.

To study the secretion of epidermic growth factor (EGF) in tears from patients with herpes simplex virus keratitis (HSVK), radio-immunoprecipitation was used to detect the value of EGF in human reflex tears from 40 cases as control group (group A), 49 cases of acute HSVK (group B) and 31 cases of convalescence HSVK (group C). Results showed that the value of EGF in reflex tears from the patients in the group B was obviously increased as compared with that in the group A and group C (P<0.001). The value of EGF in tears in the group C was higher than in group A (P<0.05). It was concluded that the value of EGF in reflex tears from HSVK patients was higher than that from normal people.

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An Experimental Study on the Effect of Hydroxypropyl Methylcellulose on Anterior Chamber

Yizhen HU ; Zijun MENG

Acta Medicinae Universitatis Scientiae et Technologiae Huazhong.2001;30(2):168-170.

The effect of hydroxyprolpyl methylcelulose (HPMC) injected into anterior chamber on filtering angle tissues was studied. Twenty-four rabbits were randomly divided into two groups: the experimental group included 40 eyes receiving HPMC which was injected into anterior chamber and the control group included 8 eyes (one served as normal control and the remaining were injected with balanced salt solution). According to the intaocular pressure (IOP) was normal or elevated ,the experimental group was further divided into group A and B. The tissul specimen of filtering angle were collected to perform pathological examination at 5 days, 3 weeks ,and 10 weeks after injection.During the early period, in groupA,the trabecular meshwork dilated slightly, the vacuoles increased in endothelial cells of inner wall of Schlemm's canal. In group B, the space of trabeculum broadened, there were accumulation of collagen and intercellular fibrosis, the vacuoles significantly increased in endothelial cells of inner wall of Schlemm's canal. But the change mentioned above all recovered to normal appearance on the later stage of long term following observationHPMC injected into anterior chamber may cause temporary elevation of IOP and pathological change of filtering angle, but the change was reversible. HPMC is not toxic or destructive to the tissues of anterior chamb angle.

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