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CHEMOTHERAPEUTIC EFFICACY OF PRAZIQUANTEL IN RATS WITH PROTECTIVE IMMUNITY TO CLONORCHIS SINENSIS INFECTION

Fushi QUAN ; Hyejeong LEE ; Myungsook CHUNG ; Joonsang LEE ; Hanjong RIM ; Kyounghwan JOO

Chinese Journal of Parasitology and Parasitic Diseases.2000;18(2):98-102.

[Objective] To study the synergic effect of praziquantel (PZQ) and host acquired immunity on Clonorchis sinensis. [Methods] Acquired immunity to C. sinensis was induced by immunization with crude adult worm antigen (AW Ag) and excretory-secretory antigen (ES Ag) or infection with C. sinensis metacercariae. The effect was assessed by the worm reduction rate compared with the control groups after challenge infection with 50 metacercariae and treated orally with a subcurative dose of praziquantel (50 mg/kg). Significant test was performed by analysis of variance (ANOVA) and Nparl way Kruskal-Wallis test. All calculations were performed by PC-SAS system. [Results] 1. PZQ was more effective against C. sinensis larvae than against adult worms in the control (P＜0. 001), ES Ag (P＜0.01) or crude AW Ag immunization group (P＜0. 001). 2. As compared with the control, the worm reduction rate after challenge infection was significantly higher (P＜0. 001) in ES Ag immunized group (35.60%) and metacercaria infection group (97.5 % ) and less in crude AW Ag group (23.4 %). The PZQ efficacy was significantly enhanced in ES Ag immunized group. [Conclusion] The efficacy of PZQ against C. sinensis could be synergically enhanced in rats by inducing host acquired immunity.

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A NMRI SUCKLING MOUSE MODEL FOR THE EVALUATION OF INFECTIVITY OF CRYPTOSPORIDIUM PARVUM OOCYSTS

Li XUNDE ; Brasseur PHILIPPE

Chinese Journal of Parasitology and Parasitic Diseases.2000;18(2):94-96.

[Objective] To evaluate the infectivity of Cryptosporidium parvum oocysts in NMRI suckling mouse.[Methods] Four-day- old SPF NMRI suckling mice were inoculated with different amounts of oocysts by oral gavage.On clay 7 after inoculation, suckling mice were sacrificed, and a suspension was prepared by homogenizing the intestinal tract from pylorus to anus. A mouse was considered infected when oocysts were found in smears of the intestinal content suspension stained with carbo lfuchsin solution. The infectivity of oocysts was evaluated as measured by the percentage of infected mice in each group. [Results] Mice receiving 1 500 or 2 000 oocysts were all infected. The percentages of infected mice were 88, 74, 51 and 28 respectively after ingestion of 1 000, 500, 250 and 100 oocysts. The percentage of infected mice was 9.5 % after ingesting as few as 50 oocysts. [Conclusion] This model is convenient for evaluation of the infectivity of C. parvum oocysts.

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IN VITRO INHIBITORY EFFECT OF INTERFERON ON SIMULATED GRANULOMA FORMATION BY EGGS OF SCHISTOSOMA MANSONI

Wucheng ZHANG

Chinese Journal of Parasitology and Parasitic Diseases.1987;0(02):-.

The effect of gamma interferon on the simulated granuloma formation induced by the eggs of Schistosoma mansoni was observed using a model of SEA-polyacrylamide beads and mouse spleen cells in vitro. The results showed that interferon at the concentration of 0.1~10U/ml could not affect significantly the granulomatous response of the spleen cells of unimmunized mice (Table 1); but at the concentration of 10-100U/ml it could inhibit significantly the response of the spleen cells of immunized mice (Table 2). The mechanism of the inhibition is probably due to the suppression of T helpers in the present study.

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SCANNING ELECTRON MICROSCOPIC OBSERVATION ON THE DAUGHTER REDIA OF PARAGONIMUS WESTERMANI

Dehua PANG

Chinese Journal of Parasitology and Parasitic Diseases.1987;0(02):-.

This paper is the first report on the ultrastructure of daughter redia of Paragonimus westermani in China. The specimens were obtained from naturally infected Semisulco-spira libertina collected from Daye, Hubei Province.The daughter redia of P. westermani is elongated or in the shape of slightly bent cylinder. The outer surface of tegument is folded in the form of annular ridges and troughs. The body surface is frequently contoured to show the outline of developing cercariae inside. At the anterior end, the daughter redia looks conical. The mouth is at the tip of the anterior end and surrounded by a thinner ring of tegument forming a distinct lip. A short distance behind the mouth there was a "birth pore" which is sur-rounded by microvillous projections, vesicles and pits.On the surface of the daughter redia, many microvillous projections are observed and numerous tiny vesicles are attached to the tegument, thus greatly increasing the absor-ption surface area of the tegument and serving in the transport of nutrients through the microvillous projections from the outer surface into the tegument.

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An Introduction to International Parasitic Germplasm Resources and Sharing

Huaimin ZHU

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

The paper selectively introduces the famous and richly historical parasite collections in the world.Over the years these institutions or museums have proven to be valuable resource for researchers and students around the world for locating information about specimen holdings,accessions,category of type specimens for each collection listed,services(including resource sharing),and background of the reservation institutes.The information presented here could provide useful references for researchers and teachers.

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Current Status,Utilization and Sharing of Mosquito Germplasm Resources in China

Fengyi QU

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

This paper reviewed the main mosquito-species resource of China recorded during 1828-2002 in different periods through Feng(1938),Meng(1955),Lu(1977),to Qu(2002).According to the new systematic series of Reinert(2001),the mosquito-record of China would be totally: 21 genera,52 subgenera,and 395 species/ subspecies up to 2006.The first report of mosquito studies in China including: new species described by foreigner or Chinese entomo-logists,check list,hand-book of "key to Chinese mosquitoes",and "Fauna of China" were cited.And with some discu-ssions on guarantee of the quality of resource materials,its utilization and resource-sharing.

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The Reservation and Use of Parasite Resources in Livestock and Poultry

Guosheng HE

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

Parasite resources are not only needed for scientific research,but also one of important national resources.This article reviewed the progress and importance of the collection and reservation of veterinary parasites,and put forward the suggestion and comments on how to construct a parasite resource platform and improve the function of the parasite resources in order to improve sharing the resources and better serve for the scientific research and control of parasitic diseases.

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Classification of Nematode in Ruminant from Qinghai Province

Jinzhong CAI

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

To classify and identified the nematode parasized in ruminant in Qinghai province,the mature nematode specimen parasized in Tibetan sheep,goat,yak,ox and camel was collected and classified systematically,by adopting classfication systems of Yamaguti(1961).All those specimens belong to 24 genera of 15 families of 6 orders in nematade of Nemathelminthes Class,of which 85 kinds of nematodes were sorted out,among them 13 lung nematodes,71 digestine nematode and 1 muscle nematode were classified.

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Collection of Parasite Samples and Preservation of Specimens

Zhengshan CHANG

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

The paper introduced standard approaches on collection of parasite samples and preservation of specimens.The collection of parasite samples included the procedures of collecting parasite samples from field or ento-surface of patient′s body infected with parasites,such as helminth,protozoan,and insect,so that the entire samples were able to be kept originally.The preservation of specimens included the procedures of fixing and preserving different stages and different hosted organs of parasites,so that whole specimens could be preserved in a long term.

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Processing Methods of Plant Nematode Slides

Yuxi DUAN ; Lijie CHEN

Chinese Journal of Parasitology and Parasitic Diseases.2006;0(S1):-.

The processing methods of plant nematode slides were introduced in this paper,including temporary mounts,permanent mounts and the stain method for nematode.Each method was described step by step in detail.These methods were essential to the study on classification,identification and characteristics of the plant nematode.

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