Neural stem cell replacement therapy: how long will it take to be applied clinically?.
Chinese Journal of Pediatrics.2006;44(7):484-486.
Chinese Journal of Pediatrics
1950 to Present ISSN: 0578-1310
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Molecular diagnosis of pediatric neurogenetic disorders: the present status and future in China.
Chinese Journal of Pediatrics.2006;44(7):481-483.
Chinese Journal of Pediatrics.2006;44(6):475-476.
Chinese Journal of Pediatrics.2006;44(6):473-474.
Adult ; Coombs Test ; Emergency Treatment ; methods ; Erythroblastosis, Fetal ; blood ; immunology ; therapy ; Erythrocyte Transfusion ; Female ; Humans ; Infant, Newborn ; Isoantibodies ; immunology ; Male ; Phototherapy ; Pregnancy ; Rh Isoimmunization ; immunology ; physiopathology ; Rh-Hr Blood-Group System ; immunology ; Rho(D) Immune Globulin ; Treatment Outcome
Robin sequence: report of two cases.
Chinese Journal of Pediatrics.2006;44(6):472-473.
Female ; Humans ; Infant ; Infant, Newborn ; Male ; Pierre Robin Syndrome ; diagnosis ; physiopathology ; therapy ; Prognosis
Diagnosis of hereditary tyrosinemia type I: clinical study of ten patients.
Chinese Journal of Pediatrics.2006;44(6):470-471.
Child, Preschool ; Female ; Humans ; Infant ; Male ; Methionine ; blood ; Tyrosine ; blood ; Tyrosine Transaminase ; deficiency ; Tyrosinemias ; blood ; diagnosis ; enzymology ; pathology ; therapy
Result of bronchoalveolar lavage fluid bacterial culture in 80 children with airway foreign body.
Chinese Journal of Pediatrics.2006;44(6):469-470.
Airway Obstruction ; complications ; diagnosis ; etiology ; surgery ; Bronchoalveolar Lavage Fluid ; microbiology ; Bronchoscopy ; Child, Preschool ; Colony Count, Microbial ; Female ; Foreign Bodies ; surgery ; Gram-Positive Bacteria ; isolation & purification ; Humans ; Infant ; Male ; Respiratory Tract Infections ; diagnosis ; etiology ; microbiology ; Staphylococcus aureus ; isolation & purification
Neuroprotective effects of melatonin against hypoxic-ischemic brain damage in neonatal rats.
Chinese Journal of Pediatrics.2006;44(6):467-468.
Animals ; Animals, Newborn ; Antioxidants ; administration & dosage ; pharmacology ; Apoptosis ; drug effects ; Brain ; drug effects ; pathology ; Disease Models, Animal ; Female ; Hypoxia-Ischemia, Brain ; drug therapy ; metabolism ; pathology ; Immunohistochemistry ; Male ; Melatonin ; administration & dosage ; pharmacology ; Neuroprotective Agents ; administration & dosage ; pharmacology ; Rats ; Rats, Sprague-Dawley
Chinese Journal of Pediatrics.2006;44(6):465-466.
Chinese Journal of Pediatrics.2006;44(6):459-464.
OBJECTIVETo investigate characteristics of pulmonary stem cells labeled with bromodeoxyuridine (Brdu) and telomerase reverse transcriptase (TERT) in lung tissue, as well as the effects of proliferation and differentiation of the stem cells on lung development and repair of pulmonary injury.
METHODSA model of hyperoxia in neonatal rats was made by exposing the rats to 95% O2 for 7 d. Before sacrificing the rats, Brdu was injected through peritoneum, and immune staining positive cells were analyzed after the rats were sacrificed. TERT positive cells were stained by an immunohistochemical method. At the same time, the double staining for surfactant protein C (SPC) and Brdu or SPC and TERT were performed. Lung histologic study was done on HE stained tissue slices.
RESULTS(1) The lung with hyperoxic injury had thinner walls of alveoli, simple alveolar structure, fewer and larger alveoli, expanded and shrunken alveoli, and there were many fell-off alveolar epithelial cells in the alveolar cavities as well. (2) The cells positively stained with Brdu located in septa, mucosa and submucosa of various bronchi, scattering in epithelium of bronchi, and the number of positive cells was low, having a large nucleus. The TERT-positive cells were apparent in the septa and alveolar walls of peripheral lung tissue, characterized by uneven distribution in the lung lobes, the number of positive cells was less than that of Brdu-positive cells [integral of expression (1.61 +/- 0.83) vs. (0.62 +/- 0.55), P < 0.05]. The number of Brdu- and TERT-positive cells had no significant difference in hyperoxic rats compared to that in controls [integral of expression (1.43 +/- 0.85) vs. (1.61 +/- 0.83); (0.62 +/- 0.55) vs. (0.83 +/- 0.84), P > 0.05]. (3) After double staining, a few positive cells were found in double-stained tissues with SPC and Brdu or TERT. (4) The cells positively stained with SPC antibody had different size. The percentage of positive cells was not significantly different between the hyperoxia group (80.3%) and control group (78.6%). The Brdu positive staining located in nucleus of cells that had larger size than the cells not stained, round nucleus with intense staining (seldom, pole-shaped) and the number of such cells was less than that of the SPC positive cells. The percentage of positive cells was not significantly different between the hyperoxia group (28.5%) and control group (21.4%). (5) The TERT staining located in nucleus of cell that had smaller size than the cells not stained, various nuclear shape, including round intensively stained, round slightly stained, pole-shaped and divided shape. The percentage of positive cells was not significantly different between the hyperoxia group (2.3%) and control group (1.5%).
CONCLUSIONS(1) Brdu and TERT, as markers of stem cells having different capability of differentiation, possess special characteristics, respectively. The cells with Brdu could be transit amplifying cell (TAC) which retains characteristics of stem cells originated from differentiated stem cells, while, the cells stained with TERT especially reflects the characteristics of stem cells. (2) The proliferation and differentiation of pulmonary stem cells during hypoxic lung injury are limited and may be related with arrest of alveolization.
Alveolar Epithelial Cells ; pathology ; Animals ; Animals, Newborn ; Biomarkers ; metabolism ; Bromodeoxyuridine ; metabolism ; Cell Differentiation ; Cell Proliferation ; Disease Models, Animal ; Female ; Hyperoxia ; complications ; Immunohistochemistry ; Lung ; cytology ; metabolism ; pathology ; Lung Injury ; etiology ; metabolism ; pathology ; physiopathology ; Male ; Peptides ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; metabolism ; pathology ; Telomerase ; metabolism
Country
China
Publisher
中华医学会
ElectronicLinks
https://www.cmaped.org.cn/Editor-in-chief
cjp@cma.org.cn
Abbreviation
Chinese Journal of Pediatrics
Vernacular Journal Title
中华儿科杂志
ISSN
0578-1310
EISSN
Year Approved
2009
Current Indexing Status
Currently Indexed
Start Year
1950
Description
历史沿革【现用刊名：中华儿科杂志;创刊时间：1950】，该刊被以下数据库收录【CA 化学文摘(美)(2009);CBST 科学技术文献速报(日)(2009);Pж(AJ) 文摘杂志(俄)(2009);中国科学引文数据库(CSCD—2008)】，核心期刊【中文核心期刊(2008);中文核心期刊(2004);中文核心期刊(2000);中文核心期刊(1996);中文核心期刊(1992)】，期刊荣誉【中科双效期刊】。
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