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The Effect of Dehydroepiandrosterone on Inhibition of Carcinogenesis and Induction of Apoptosis in Murine Hepatoma Model.

Kye Yong SONG ; Eun Sup PARK ; Jee young CHOI ; Sang Chul PARK

Korean Journal of Pathology.1995;29(1):24-32.

Tumor suppressive effect of dehydroepiandrosterone (DHEA) on the experimentally induced hepatocellular carcinoma was investigated, especially focusing on glutatione transferase and transglutaminase with aptosis in the carcinogenesis. The chemical hepatocarcinogenic procedure of Solt-Farber method was used on Sprague-Dawley rats. Experimental groups were divided into AA group treated by the standard Solt-Farber regimen of diethylnitrosamine (DEN) and 2-acetamidofluorene (AAF) and AD group treated with DHEA simultaneously with AAF and the AAD group treated by DHEA after treatment with AAF. Each group was divided by time sequence further into four subgroups, GI (8wk), G2 (16wk), G3 (28wk), and G4 (36wk). For neoplastic lesion, the immuno histochemical study with anti GSTP antibody was carried out, while the activity and expression of TGase was compared at the same time. The results were summarized as follows; GST-P positive foci detected in AD groups were significantly more suppressed by DHEA treatment than AA groups (P<0.05). AD groups. AD group showed higher activities of TGase than AA groups (P<0.05), which was confirmed by Western and Northern blot analysis. But the number of apoptotic bodies was not correlated with activity and expression of TGase in the nodule. These results suggest that the suppressive effect of DHEA on the murine hepatocellular carcinogenesis might be operating on the promotion process of carcinogenesis rather than regression process of transformed hyperplastic nodules.
Carcinoma, Hepatocellular

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Ultrastructural Study of Amiodarone-Associated Lung Injury.

Eun Yung KIM ; Sang Han LEE ; Yoon Kyung SOHN ; Tae Joong SOHN

Korean Journal of Pathology.1995;29(1):10-23.

Amiodarone, an antiarrhythmic drug, may exert pulmonary toxicity in some patients but the pathogenesis is not clear. This study was carried out to investigate the pathogenetic mechanism of pulmonary injury induced by amiodarone at dose of 100 mg/kg/day given to rats by intraperitoneal injection for 3 weeks. And the preventive effects of concomitantly injected steroid (10 mg/kg/day) on amiodarone induced pulmonary injury was also studied using bronchoalveolar lavage, light microscopy and transmission electron microscopy. The results obtained were summarized as follows: Mild lymphocytosis of bronchoalveolar lavage fluid was found in all experimental groups. Intracytoplasmic lamellar body formation was found in all types of pulmonary cells and type II pneumocytes revealed the earliest abnormal lamellar body formation. The capillary endothelial cells showed cellular swelling and detachment from underlying basement membrane at early phase of experiment and the edema of alveolar wall and interstitium were noted. Interstitial fibrosis and proliferation of type II pneumocytes were noted at late phase. The lungs of steroid injected groups revealed accumulation of lamellar bodies in all types of pulmonary cells but interstitial fibrosis was not occurred. These findings support the concept that amiodarone is responsible for a drug-induced phospholipidosis and directly toxic to pulmonary endothelial and epithelial cells. And steroid may regress the progression of amiodarone induced pulmonary injury.
Rats ; Animals

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The Role of Ito Cell in Hepatic Fibrosis after Common Bile Duct Ligation: inhibitory role of vitamin A in Ito cell.

Kyung Hee PARK ; Sang Han LEE ; Jong Min CHAE

Korean Journal of Pathology.1995;29(1):1-9.

The purpose of this study was to investigate the inhibitory role of vitamin A with respect to activation of Ito cells in fibrosis of the rat liver induced by common bile duct ligation(CBDL). The liver was examined by immunohistochemical staining for a-smooth muscle actin,the known marker of activated Ito cells, and light and electron microscopy after CBDL andCBDL with intraperitoneal injection of retinoic acid (Sigma, USA) 1 mg/Kg in 3 times per week. The results were sumrrlerized as follows: After CBDL, the bile ductules were markedly proliferated in the periportal areas extending toterminal hepatic veins. Interstitial fibrosis and inflammatory cell infiltration appeared, however,cholestasis was minimal. Retinoic acid treatment with CBDL decreased bile ductular proliferationand interstitial fibrosis compared to CBDL only. After CBDL, proliferated and activated Ito ceIs showing positive reaction in smooth muscle actin were present in the periductular andperisinusoidal areas, and areas of increased interstitial fibrosis. Activated ito cells weredecreased in number after CBDL with vitamin A treatment. Electron microscopically,intracytoplasmic fat droplets and the cytoplasmic processes of Ito cells were decreased afterCBDL. Myofibroblasts were frequently appeared in the interstitial fibrosis after CBDL. But,intracytoplasmic fat droplets of Ito cells were well preserved, and myofibroblasts were found lessfrequently after CBDL with vitamin A treatment. The results suggest that vitamin A plays an inbitory role in the activation and fibrogenesis ofIto cells after CBDL.
Rats ; Animals

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Multiple Proliferating Trichilemmal Tumors with Ordinary Trichilemmal Cysts: A case report.

In Sook KIM ; Jin Hee SOHN ; Hye Seon AHN ; Jung Il SUH ; Hyo Sook PARK ; Soo Jo KIM ; Sook Ja SOHN

Korean Journal of Pathology.1987;21(3):207-213.

Proliferating trichilemmal tumor is one of rare benign tumors of the skin appendages, considering as hamartoma of the terminal hair follicle, isthmic segment of the outer root sheath. We report a case of numerous proliferating trichilemmal tumors admixed with ordinary trichilemmal cysts with femilial history in 64-year-old man. He has total 157 lesions, which are chiefly located in scalp (48), back (32), and also face, neck, trunk, extremities. Histologically, the tumor is composed of irregularly arranged and ansatomosed trabeculae, lobules, or sheets of proliferated trichilemmal squamous epithelium with peripheral palisading of the basaloid cells. Several layers of squamoid or ovoid cells have PAS-positive clear cytoplasm. And it also shows abrupt amorphous, trichilemmal keratinization. Some squamous eddies with mild cellular atypism are associated, but definite invasion or other evidence of the malignancy is not found. usual multiple trichilemmal cysts are admixed with tumor and occasionally exhibit connection between them.
Cysts ; Hamartoma

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Detection of Chromosome Aberration in Interphase Nuclei of Tumor Cells by Nonradioactive In Situ Hybridization Using Chromosome-specific Probes.

Sang Sook LEE

Korean Journal of Pathology.1993;27(6):573-580.

An improved protocol for in situ hybridization(ISH) to routinely processed, paraffin-embedded tissue sections from lung carcinoma is presented. For this study, DNA probes for alpha-satellite chromosome 7 and 17 were used. The protocol to detect numerical chromosome aberrations involved treatment of sections with 1 M sodium thiocyanate prior to pepsin digestion, resulting in reproducible ISH reactions. The effect of avidin-biotin detection system. Four layer avidin methods and triple biotin methods, using avidin-PO, goat antiavidin, biotinylated antigoat IgG, avidin-PO or anti-biotin, biotinylated antirabbit IgG, avidin-PO, markedly enhanced the intensity of positive signals. More than 80% of the tumor and stromal cells showed distinct chromosome hybridization signals in 6 micrometer-thick sections. Lung carcinoma cells showed multiple chromosome signals(2~5 spots), contrasted by one or two signals in the stromal cells in the same section. These results suggest that chromosome polysomy can be reliably detected in tissue sections using in situ hybridization. This capability will prove to be an important tool for determining the underlying genetic basis for tumor development, tissue phenotype heterogeneity and progression by allowing genetic determination to be made on paraffin-embedded tissue sections where tumor histologic architecture is preserved.

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A Pathological Study of Phenol Induced Hepatic and Gastrointestinal Lesions.

Dae Young KANG

Korean Journal of Pathology.1993;27(6):561-572.

In an attempt to elucidate the pathological effects of phenol, the present study was undertaken in male Sprague-Dawley rats. The control group of animals was fed a basal diet, and potable underground water. The experimental group of animals was fed a basal diet and potable underground water containing 30ppm, 60ppm, and 1% phenol with once a week administration of dimethylnitrosamine(DMN) 10 mg/kg I.P. Each group of animals was sacrificed on the 3rd, 6th, and 9th month. The liver and gastrointestinal tract were examined light microscopically, along with transmission electron microscopic studies of the liver and scanning electron microscopic studies of the gastric mucosa. The results were as follows: 1) In the acute phenol intoxicated group, the liver showed fatty changes in the hepatocytes with mitochondrial membrane destruction and myelin figure formation. 2) In the chronic phenol intoxicated group, fatty changes in the liver were observed. In addition, there was chronic inflammation in the gastrointestinal tract, with gastric mucosal erosion and central necrosis of the hepatic lobules, especially in the high phenol contaminated water treated group. 3) As a result of the examination under the light microscope, the DMN treated group showed hyperplastic nodules and liver cell dysplasia, the degree of which was proportional to the duration of the experiment, and was more severe in the DMN + phenol treated group. 4) As a result of the examination under the electron microscope, fatty changes in the liver, pleomorphism of the mitochondria and loss or shortness of bile canalicular microvilli in the DMN + phenol treated group were more severe than in the group treated only with DMN. In summary, the results obtained by the present study indicate chronic highly concentrated phenol intoxication induce liver cell necrosis and chronic inflammatory with a hepatotoxin such as DMN.
Male ; Humans ; Animals

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Application of Argyrophilic Nucleolar Organizer Regions(AgNORs) in the Diagnosi of Hepatocellular Carcinoma.

Cheol Hee YUN ; Sang Sook LEE ; Eun Sook CHANG

Korean Journal of Pathology.1993;27(6):553-560.

Necleolar organizer regions(NORs) ARE LOOPS OF DNA which transcribe to ribosomal RNA by RNA polymerase I. Since NOR-associated proteins are argyrophilic, silver staining method has been used for demonstration of AgNORs. The numbers and/or configurations of NORs may reflect the activities of cells in hyperplastic and neoplastic conditions. To evaluated the applicability of AgNORs in the diagnosis of hepatocellular carcinoma, the author had performed silver staining on the routinely processed, formalin-fixed, paraffin-embedded sections of 14 cases of normal liver(control), 23 cirrhotic liver, and 21 hepatocellular carcinoma. The results are summarized as follows: 1) The mean number of AgNORs per nucleus(mAgNOR) of normal liver, cirrhotic liver and hepatocellular carcinoma was 1.45+/-0.07, 2.53+/-0.38 and 5.52+/-1.63, respectively. The difference of mAgNOR between normal and cirrhotic liver, and between cirrhotic liver and hepatocellular carcinoma was statistically significant, respectively(p<0.01). 2) The percentage of nuclei showing five or more AgNORs per nucleus(pAgNOR) was 0.07% in normal liver, 7.59% in cirrhotic liver, and 60.49% in hepatocellular carcinoma. 3) AgNORs in hepatocellular carcinoma were large, pleomorphic and irregularly clumped, in addition to increase of mAgNOR and high pAgNOR. In conclusion, the increase of mAgNOR, high pAgNOR and large, irregular AgNORs are regarded as an additional helpful finding for the histopathological diagnosis of hepatocellular carcinoma.
Carcinoma, Hepatocellular

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Cytologic Distinctive Features of Brenner Tumor.

Jung Sik JANG ; An Na SEO ; Seon Jae LEE ; Ji Young PARK

Korean Journal of Pathology.2011;45(2):223-226. doi:10.4132/KoreanJPathol.2011.45.2.223

Herein, we present two cases of Brenner tumor, a rarely occurring neoplasm in the ovaries, obtained via intraoperative fine needle aspiration. The borderline Brenner tumor exhibited marked squamous metaplasia, characterized by individually distributed atypical squamous cells. A benign Brenner tumor associated with mucinous cystadenoma evidenced typical mucinous metaplastic features and transitional foci. These distinctive features may prove helpful in differential diagnosis of varied ovarian tumors, and particularly for intraoperative consultation.
Biopsy, Fine-Needle ; Brenner Tumor ; Cystadenoma, Mucinous ; Diagnosis, Differential ; Female ; Metaplasia ; Mucins ; Ovary

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Fine Needle Aspiration Cytologic Findings of Angiosarcoma: Report of Two Cases.

Jin Xian JI ; Young Chae CHU ; Lucia KIM ; Suk Jin CHOI ; In Suh PARK ; Jee Young HAN ; Joon Mee KIM ; Kyu Ho KIM ; Ju Young SONG

Korean Journal of Pathology.2011;45(2):217-222. doi:10.4132/KoreanJPathol.2011.45.2.217

Angiosarcoma is a rare malignant vascular neoplasm which can arise in any part of the body. Specific recognition of this neoplasm in cytological specimens is difficult in the absence of an ancillary method. Herein, we present the cytologic findings of two cases of angiosarcomas diagnosed on fine needle aspiration cytology. One case is a recurred angiosarcoma in the left chest wall and the other case is a lymphedema-associated angiosarcoma in the left lower leg. The cytologic findings of both cases are similar. Cytologic features that identified this neoplasm as an angiosarcoma included arborizing microtissue fragments, irregular anastomosing vascular spaces lined by atypical cells, microacini, intracytoplasmic lumen, and intracellular red blood cells, marked cell discohesiveness, spindle to ovoid, irregular, hyperchromatic nuclei, and elongated cytoplasmic processes with indistinct borders. This report emphasizes that when aspiration smears show vasoformative features in a bloody background, angiosarcoma should be included in the differential diagnosis.
Biopsy, Fine-Needle ; Cytoplasm ; Diagnosis, Differential ; Erythrocytes ; Hemangiosarcoma ; Leg ; Thoracic Wall ; Vascular Neoplasms

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Functional Adrenocortical Oncocytoma: A Case Report of Rare Neoplasm of Uncertain Malignant Potential.

Jamshid ABDUL-GHAFAR ; Keum Seok BAE ; Kwang Hwa PARK

Korean Journal of Pathology.2011;45(2):212-216. doi:10.4132/KoreanJPathol.2011.45.2.212

Adrenocortical oncocytoma is a rare adrenal neoplasm with only 25 cases having been reported in the English medical literature, of which only seven were functional tumors. Since these adrenal tumors are usually nonfunctional, they are mostly incidentally detected, and most of them are benign. Herein, we report on a rare case of a functional adrenocortical oncocytoma of an uncertain malignant potential and this tumor was located in the left adrenal gland in a 59-year-old woman who presented with hypertension. The tumor size was large with foci of necrosis in the cut surface and it exclusively had oncocytic histologic features.
Adenoma ; Adenoma, Oxyphilic ; Adrenal Gland Neoplasms ; Adrenal Glands ; Female ; Humans ; Hypertension ; Middle Aged ; Necrosis

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